Hoeflea sp. IMCC20628

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Hoeflea; unclassified Hoeflea

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7PMN7|A0A0F7PMN7_9RHIZ Universal stress protein UspA-like protein OS=Hoeflea sp. IMCC20628 OX=1620421 GN=IMCC20628_01705 PE=3 SV=1
MM1 pKa = 7.52SLLTDD6 pKa = 4.35SIDD9 pKa = 5.07AITFDD14 pKa = 4.87LGPDD18 pKa = 3.33EE19 pKa = 4.76TPIEE23 pKa = 4.57SINTSGASPYY33 pKa = 11.04VMNFQYY39 pKa = 11.09ASSQPADD46 pKa = 3.14LWNTYY51 pKa = 7.84TGWTSFSAAEE61 pKa = 3.9KK62 pKa = 8.75ATYY65 pKa = 9.82RR66 pKa = 11.84GLLDD70 pKa = 3.69YY71 pKa = 11.39VEE73 pKa = 4.32TLINVTFQEE82 pKa = 4.64VSGSSDD88 pKa = 3.17PDD90 pKa = 3.48MNVGKK95 pKa = 10.14VQIDD99 pKa = 3.5DD100 pKa = 3.83AAGKK104 pKa = 10.73GGFQYY109 pKa = 11.06YY110 pKa = 7.97YY111 pKa = 7.65TTSGQTATLTDD122 pKa = 3.68YY123 pKa = 11.47DD124 pKa = 3.95AFTVFEE130 pKa = 4.37NDD132 pKa = 2.64ISLEE136 pKa = 4.12SGSADD141 pKa = 4.68LILHH145 pKa = 6.82EE146 pKa = 5.39IGHH149 pKa = 6.96ALTLKK154 pKa = 10.5HH155 pKa = 6.46PFSGSPVLPTAYY167 pKa = 10.34DD168 pKa = 3.58NNKK171 pKa = 7.4YY172 pKa = 9.3TIMSYY177 pKa = 10.2TSNPDD182 pKa = 2.99TGLDD186 pKa = 3.55NEE188 pKa = 5.38GYY190 pKa = 10.47LAFDD194 pKa = 3.96IAALQQRR201 pKa = 11.84WGANMNTATGNDD213 pKa = 4.3VYY215 pKa = 10.34TGPRR219 pKa = 11.84IPTVDD224 pKa = 4.25VIWDD228 pKa = 3.41AGGTDD233 pKa = 3.37KK234 pKa = 11.33LSAFGYY240 pKa = 8.39STDD243 pKa = 3.46VLIDD247 pKa = 3.85LNEE250 pKa = 4.21GAFSRR255 pKa = 11.84FGTNDD260 pKa = 3.26DD261 pKa = 3.3VAIAYY266 pKa = 8.45GVVIEE271 pKa = 4.63NATGGNSHH279 pKa = 7.21DD280 pKa = 5.08LIIGNAQANTLAGGNGNDD298 pKa = 3.68NLSGGGGNDD307 pKa = 3.36YY308 pKa = 10.95FIGGNGIDD316 pKa = 4.05TYY318 pKa = 11.54DD319 pKa = 3.94GGSGSDD325 pKa = 3.13TARR328 pKa = 11.84FDD330 pKa = 4.75GNVGWRR336 pKa = 11.84VDD338 pKa = 4.08LGTGTAKK345 pKa = 10.54VGSQTEE351 pKa = 4.18MLTSIEE357 pKa = 4.16NVVGSNGNDD366 pKa = 3.66TITGTAYY373 pKa = 11.18SNDD376 pKa = 3.81LYY378 pKa = 11.3GGNGDD383 pKa = 4.68DD384 pKa = 3.98MLIGGGGIDD393 pKa = 4.52DD394 pKa = 4.55YY395 pKa = 11.75YY396 pKa = 11.29GGSGSDD402 pKa = 3.43TVDD405 pKa = 3.49LSGSNSKK412 pKa = 9.3WRR414 pKa = 11.84VDD416 pKa = 3.77LGNDD420 pKa = 3.37TAEE423 pKa = 4.23MTTATGNVYY432 pKa = 10.64NVDD435 pKa = 4.17FEE437 pKa = 5.11NIEE440 pKa = 4.35SVIGPRR446 pKa = 11.84NSLTLLGTSGNEE458 pKa = 3.74EE459 pKa = 3.6ARR461 pKa = 11.84GNSFADD467 pKa = 2.94IFYY470 pKa = 9.94TRR472 pKa = 11.84GGVDD476 pKa = 3.79EE477 pKa = 4.33FHH479 pKa = 7.0GSYY482 pKa = 11.14GSDD485 pKa = 2.68TVSFSGYY492 pKa = 10.99GEE494 pKa = 4.33GLTIRR499 pKa = 11.84GSAGYY504 pKa = 10.17GRR506 pKa = 11.84LGDD509 pKa = 3.88GTQVTTFTGIEE520 pKa = 4.2NIFATSEE527 pKa = 4.04DD528 pKa = 4.03DD529 pKa = 6.04DD530 pKa = 3.94ITGTAGNNTIVGRR543 pKa = 11.84SGADD547 pKa = 3.5RR548 pKa = 11.84INGGSGNDD556 pKa = 3.61IIQGGLGRR564 pKa = 11.84DD565 pKa = 3.61LLTGGRR571 pKa = 11.84GNDD574 pKa = 3.93TIDD577 pKa = 4.24GGSDD581 pKa = 3.23PNDD584 pKa = 3.14YY585 pKa = 11.53AFFSGNQSQYY595 pKa = 11.33NISTSAGGVTTVEE608 pKa = 4.27WIGPGNGDD616 pKa = 3.78GTDD619 pKa = 3.04TLTNIEE625 pKa = 4.67FLGFDD630 pKa = 3.38DD631 pKa = 4.33GFYY634 pKa = 11.03YY635 pKa = 10.73VV636 pKa = 4.22

Molecular weight: 66.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7PRS7|A0A0F7PRS7_9RHIZ Transcriptional regulator TetR family OS=Hoeflea sp. IMCC20628 OX=1620421 GN=IMCC20628_03186 PE=4 SV=1
MM1 pKa = 7.47TSGNRR6 pKa = 11.84LKK8 pKa = 10.85KK9 pKa = 10.18LRR11 pKa = 11.84KK12 pKa = 8.91QAAQRR17 pKa = 11.84QGGYY21 pKa = 10.08CFYY24 pKa = 10.61CRR26 pKa = 11.84YY27 pKa = 9.69PMWDD31 pKa = 3.16SRR33 pKa = 11.84PEE35 pKa = 3.99EE36 pKa = 4.09FIGRR40 pKa = 11.84YY41 pKa = 9.27GISSGLAQRR50 pKa = 11.84FQCTAEE56 pKa = 4.16HH57 pKa = 5.52VQARR61 pKa = 11.84CDD63 pKa = 3.5GGKK66 pKa = 10.04DD67 pKa = 3.28VAPNIVAACRR77 pKa = 11.84FCNSTRR83 pKa = 11.84HH84 pKa = 5.42KK85 pKa = 10.52AKK87 pKa = 10.27HH88 pKa = 5.58AADD91 pKa = 3.43AQTYY95 pKa = 8.65VSFVRR100 pKa = 11.84SRR102 pKa = 11.84LAKK105 pKa = 10.28GKK107 pKa = 7.66WHH109 pKa = 6.91PPQLRR114 pKa = 11.84ASFSEE119 pKa = 4.13RR120 pKa = 11.84KK121 pKa = 9.07NASDD125 pKa = 3.12RR126 pKa = 11.84SSRR129 pKa = 11.84ARR131 pKa = 3.29

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4739

0

4739

1486412

26

2994

313.7

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.712 ± 0.041

0.876 ± 0.011

5.948 ± 0.03

5.672 ± 0.036

3.934 ± 0.023

8.462 ± 0.039

2.048 ± 0.02

5.757 ± 0.03

3.625 ± 0.028

9.795 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.016

2.86 ± 0.02

4.851 ± 0.024

3.048 ± 0.018

6.277 ± 0.035

5.933 ± 0.025

5.511 ± 0.024

7.305 ± 0.03

1.315 ± 0.015

2.27 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski