Pepper golden mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QQA4|Q8QQA4_9GEMI Nuclear shuttle protein OS=Pepper golden mosaic virus OX=223301 GN=BR1 PE=3 SV=1
MM1 pKa = 7.34EE2 pKa = 6.2AGLASAPSAFNYY14 pKa = 10.39LEE16 pKa = 4.01SHH18 pKa = 6.52RR19 pKa = 11.84DD20 pKa = 3.56EE21 pKa = 4.67YY22 pKa = 11.24QLSHH26 pKa = 7.5DD27 pKa = 3.72LTEE30 pKa = 4.56IVLQFPSTASQLTARR45 pKa = 11.84LSRR48 pKa = 11.84SCMKK52 pKa = 9.84IDD54 pKa = 3.26HH55 pKa = 6.45CVIEE59 pKa = 4.31YY60 pKa = 9.79RR61 pKa = 11.84QQVPINASGSVIVEE75 pKa = 3.61IHH77 pKa = 6.0DD78 pKa = 3.94QRR80 pKa = 11.84MTDD83 pKa = 3.53NEE85 pKa = 4.36SLQASWTFPIRR96 pKa = 11.84CNIDD100 pKa = 2.83LHH102 pKa = 6.2YY103 pKa = 10.34FSSSFFSLKK112 pKa = 10.82DD113 pKa = 3.76PIPWRR118 pKa = 11.84LYY120 pKa = 10.45YY121 pKa = 10.11RR122 pKa = 11.84VSDD125 pKa = 3.96TNVHH129 pKa = 5.21QRR131 pKa = 11.84THH133 pKa = 5.37FAKK136 pKa = 10.85FKK138 pKa = 10.96GKK140 pKa = 10.52LKK142 pKa = 10.82LSTAKK147 pKa = 10.37HH148 pKa = 5.69SVDD151 pKa = 2.95IPFRR155 pKa = 11.84APTVKK160 pKa = 10.11ILSKK164 pKa = 10.54QFSHH168 pKa = 6.93NDD170 pKa = 2.75VDD172 pKa = 5.21FSHH175 pKa = 7.27VDD177 pKa = 3.14YY178 pKa = 10.91GQWEE182 pKa = 4.4RR183 pKa = 11.84KK184 pKa = 6.96VLRR187 pKa = 11.84SNSMSRR193 pKa = 11.84VGLTGPIEE201 pKa = 4.01LRR203 pKa = 11.84PGEE206 pKa = 4.29SWASRR211 pKa = 11.84STVGTSSPSRR221 pKa = 11.84DD222 pKa = 3.4SKK224 pKa = 8.38MHH226 pKa = 7.21PYY228 pKa = 10.29RR229 pKa = 11.84EE230 pKa = 4.07LHH232 pKa = 5.59QLGPSVLDD240 pKa = 4.4PGDD243 pKa = 3.79SASQVGLQRR252 pKa = 11.84AHH254 pKa = 6.7SNITMSMAQLNEE266 pKa = 4.01LVRR269 pKa = 11.84TTVHH273 pKa = 5.94EE274 pKa = 4.82CISNNCNPAQPKK286 pKa = 10.02SLQQ289 pKa = 3.27

Molecular weight: 32.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q88884|Q88884_9GEMI Transcriptional activator protein OS=Pepper golden mosaic virus OX=223301 PE=3 SV=1
MM1 pKa = 8.25DD2 pKa = 4.26SRR4 pKa = 11.84TGEE7 pKa = 4.47SITVHH12 pKa = 4.76QAEE15 pKa = 4.03NSVFIWEE22 pKa = 4.39VPNPLFFRR30 pKa = 11.84IQHH33 pKa = 5.53VEE35 pKa = 3.99DD36 pKa = 3.8PLFTRR41 pKa = 11.84TRR43 pKa = 11.84IYY45 pKa = 10.43HH46 pKa = 4.83IVVRR50 pKa = 11.84FNHH53 pKa = 5.51NLRR56 pKa = 11.84RR57 pKa = 11.84ALALHH62 pKa = 6.42KK63 pKa = 10.65AFLHH67 pKa = 5.06FQVWTTSMTASGTTYY82 pKa = 10.72LSRR85 pKa = 11.84FKK87 pKa = 10.77FRR89 pKa = 11.84VMLYY93 pKa = 10.0LHH95 pKa = 6.33TLGAIGINNVIRR107 pKa = 11.84AVRR110 pKa = 11.84FATDD114 pKa = 3.02KK115 pKa = 11.32SYY117 pKa = 11.89VNYY120 pKa = 10.19VLEE123 pKa = 4.0NHH125 pKa = 6.63EE126 pKa = 4.81IKK128 pKa = 10.75CKK130 pKa = 10.61LYY132 pKa = 10.81

Molecular weight: 15.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1405

129

348

234.2

26.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.836 ± 0.67

2.064 ± 0.211

5.053 ± 0.371

3.986 ± 0.349

4.555 ± 0.5

5.053 ± 0.412

4.128 ± 0.371

5.694 ± 0.553

6.05 ± 0.739

6.477 ± 0.626

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.407

5.623 ± 0.406

5.267 ± 0.823

3.843 ± 0.462

6.975 ± 0.894

8.968 ± 1.296

4.982 ± 0.543

7.189 ± 1.036

1.495 ± 0.225

4.27 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski