Mycobacterium phage Henu3 PeY-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W3V7|A0A481W3V7_9CAUD Portal protein OS=Mycobacterium phage Henu3 PeY-2017 OX=2571241 GN=Henu3_gp1 PE=4 SV=1
MM1 pKa = 7.82GEE3 pKa = 4.37PEE5 pKa = 5.42DD6 pKa = 3.94PTEE9 pKa = 4.09LAGVADD15 pKa = 5.09ADD17 pKa = 4.13TMSAYY22 pKa = 9.87AWSVEE27 pKa = 3.79DD28 pKa = 3.46PTINSEE34 pKa = 4.8LIDD37 pKa = 4.21AGSDD41 pKa = 3.22RR42 pKa = 11.84PFWITTAAVGLSLSLVAVVGVLGYY66 pKa = 9.98RR67 pKa = 11.84VWVDD71 pKa = 3.29VPEE74 pKa = 4.65RR75 pKa = 11.84PTVVVAATPTTTTEE89 pKa = 3.95VEE91 pKa = 4.2AASPLPLPPPVTVTTVVVQTPAPAPAPVPVPEE123 pKa = 4.97VIPSVLPTPLPPLSNTDD140 pKa = 3.3MVFLSRR146 pKa = 11.84MRR148 pKa = 11.84AQGWYY153 pKa = 10.37VSDD156 pKa = 4.2PQLMAYY162 pKa = 10.16RR163 pKa = 11.84AFEE166 pKa = 4.09TCAMLRR172 pKa = 11.84NGEE175 pKa = 4.45PVWQVQGKK183 pKa = 9.02LLGLEE188 pKa = 4.23GVPNEE193 pKa = 4.14VEE195 pKa = 3.55ASKK198 pKa = 10.7FLNTAMSTYY207 pKa = 10.04PNCPP211 pKa = 3.13

Molecular weight: 22.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W3X6|A0A481W3X6_9CAUD Uncharacterized protein OS=Mycobacterium phage Henu3 PeY-2017 OX=2571241 GN=Henu3_gp43 PE=4 SV=1
MM1 pKa = 7.31LVLRR5 pKa = 11.84RR6 pKa = 11.84GSPRR10 pKa = 11.84GTASIKK16 pKa = 10.61PPTPSRR22 pKa = 11.84NAPSPTPTSIGRR34 pKa = 11.84SANSKK39 pKa = 7.62QTSTPTSPTSRR50 pKa = 11.84QRR52 pKa = 11.84SVSALRR58 pKa = 11.84NCSNTSRR65 pKa = 11.84KK66 pKa = 9.69NDD68 pKa = 3.58GNRR71 pKa = 11.84KK72 pKa = 8.17RR73 pKa = 11.84CSGRR77 pKa = 11.84HH78 pKa = 4.13STSDD82 pKa = 2.86QADD85 pKa = 3.66RR86 pKa = 11.84HH87 pKa = 5.66RR88 pKa = 11.84RR89 pKa = 11.84PCGNTHH95 pKa = 5.8KK96 pKa = 9.03TQTHH100 pKa = 6.45EE101 pKa = 4.07PQQTPPHH108 pKa = 6.98PLQAHH113 pKa = 6.32HH114 pKa = 7.02RR115 pKa = 11.84LAPPSHH121 pKa = 6.66PTPHH125 pKa = 6.09TTTPTNPPTHH135 pKa = 6.26QNPHH139 pKa = 5.64LRR141 pKa = 11.84TPRR144 pKa = 11.84RR145 pKa = 11.84MGQRR149 pKa = 11.84HH150 pKa = 5.82RR151 pKa = 11.84STHH154 pKa = 5.4RR155 pKa = 11.84RR156 pKa = 11.84HH157 pKa = 4.89AHH159 pKa = 5.43KK160 pKa = 10.32LARR163 pKa = 11.84LPRR166 pKa = 11.84RR167 pKa = 11.84TTQRR171 pKa = 11.84NPTTPRR177 pKa = 11.84KK178 pKa = 8.54RR179 pKa = 11.84TKK181 pKa = 8.77TNHH184 pKa = 5.71RR185 pKa = 11.84RR186 pKa = 11.84LEE188 pKa = 4.01IPRR191 pKa = 11.84TTLRR195 pKa = 11.84TTHH198 pKa = 6.22PTRR201 pKa = 11.84HH202 pKa = 6.01PRR204 pKa = 11.84RR205 pKa = 11.84PQRR208 pKa = 11.84TTT210 pKa = 2.68

Molecular weight: 23.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

20957

69

1224

220.6

24.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.791 ± 0.464

1.546 ± 0.182

5.602 ± 0.181

4.853 ± 0.271

3.011 ± 0.21

8.551 ± 0.5

2.987 ± 0.273

3.937 ± 0.227

2.729 ± 0.18

7.616 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.124

3.483 ± 0.156

6.886 ± 0.237

3.588 ± 0.187

8.37 ± 0.549

6.847 ± 0.342

6.814 ± 0.268

7.191 ± 0.298

1.999 ± 0.162

2.095 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski