Capybara microvirus Cap1_SP_82
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVN2|A0A4V1FVN2_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_82 OX=2584793 PE=4 SV=1
MM1 pKa = 7.63 SIFDD5 pKa = 3.3 IHH7 pKa = 6.77 KK8 pKa = 10.3 LKK10 pKa = 10.98 NSPTWSEE17 pKa = 3.75 FDD19 pKa = 2.81 ISQNKK24 pKa = 8.95 KK25 pKa = 8.66 FSSKK29 pKa = 9.82 IGQILPVAYY38 pKa = 8.56 RR39 pKa = 11.84 TVLPGDD45 pKa = 3.84 KK46 pKa = 10.56 VNLPISHH53 pKa = 7.0 FTQTLPLNKK62 pKa = 9.91 NSFARR67 pKa = 11.84 IRR69 pKa = 11.84 EE70 pKa = 4.12 HH71 pKa = 7.77 VDD73 pKa = 2.86 VFFVPFRR80 pKa = 11.84 LLYY83 pKa = 10.87 SKK85 pKa = 11.43 ANDD88 pKa = 2.97 ILVNNTDD95 pKa = 3.47 SQVALANSATNNIIPTEE112 pKa = 4.11 LPFTTISILNSAYY125 pKa = 9.78 KK126 pKa = 10.29 FYY128 pKa = 10.58 KK129 pKa = 10.5 AEE131 pKa = 4.09 EE132 pKa = 4.24 ASPSYY137 pKa = 9.68 TFKK140 pKa = 11.01 GHH142 pKa = 7.46 DD143 pKa = 2.93 IAGFSRR149 pKa = 11.84 AEE151 pKa = 3.76 NMKK154 pKa = 9.93 YY155 pKa = 10.2 LCEE158 pKa = 3.84 LLGYY162 pKa = 10.95 GDD164 pKa = 3.57 ITRR167 pKa = 11.84 GYY169 pKa = 9.73 ADD171 pKa = 3.82 SEE173 pKa = 4.54 GSVDD177 pKa = 3.97 NKK179 pKa = 10.96 AHH181 pKa = 6.78 IPLSMMPICAYY192 pKa = 10.1 QKK194 pKa = 10.01 IYY196 pKa = 11.32 ADD198 pKa = 3.89 FFRR201 pKa = 11.84 YY202 pKa = 7.74 QQWEE206 pKa = 4.21 DD207 pKa = 3.62 NNPYY211 pKa = 9.85 TYY213 pKa = 11.33 NFDD216 pKa = 3.57 YY217 pKa = 10.87 KK218 pKa = 10.38 IASNGMQVSLQEE230 pKa = 3.51 ITLPGFGANIYY241 pKa = 10.96 GNGIFDD247 pKa = 5.2 LRR249 pKa = 11.84 YY250 pKa = 9.33 KK251 pKa = 10.67 NYY253 pKa = 10.71 NKK255 pKa = 10.45 DD256 pKa = 3.51 YY257 pKa = 10.95 YY258 pKa = 10.96 FGLLPSQVNDD268 pKa = 2.81 IYY270 pKa = 11.26 LPKK273 pKa = 10.24 FDD275 pKa = 4.31 MTLDD279 pKa = 3.63 TEE281 pKa = 4.32 KK282 pKa = 10.45 ATEE285 pKa = 3.98 EE286 pKa = 4.09 GHH288 pKa = 5.23 FQRR291 pKa = 11.84 FNDD294 pKa = 3.09 ITTTLSVLQLRR305 pKa = 11.84 ASYY308 pKa = 10.98 ALQKK312 pKa = 10.01 WSEE315 pKa = 3.96 IQLFSDD321 pKa = 4.15 KK322 pKa = 10.89 NYY324 pKa = 9.84 KK325 pKa = 10.44 DD326 pKa = 3.66 KK327 pKa = 10.93 IKK329 pKa = 10.44 HH330 pKa = 5.75 HH331 pKa = 6.78 FNVDD335 pKa = 3.34 VPDD338 pKa = 3.68 YY339 pKa = 9.47 TANQAQYY346 pKa = 10.45 LGGYY350 pKa = 8.77 SSVMQINDD358 pKa = 3.17 IVNTNLAQANAEE370 pKa = 3.98 RR371 pKa = 11.84 KK372 pKa = 10.18 AIGNSANSGHH382 pKa = 6.89 INFDD386 pKa = 3.9 CKK388 pKa = 10.32 EE389 pKa = 4.1 HH390 pKa = 6.63 GIIMAVYY397 pKa = 8.88 YY398 pKa = 10.12 ALPLMDD404 pKa = 4.71 YY405 pKa = 8.07 GTLYY409 pKa = 10.86 GYY411 pKa = 10.57 DD412 pKa = 3.6 PEE414 pKa = 4.63 VTHH417 pKa = 5.73 VTADD421 pKa = 3.84 DD422 pKa = 4.03 FPKK425 pKa = 10.79 PEE427 pKa = 4.29 FDD429 pKa = 4.18 NLGLSPVYY437 pKa = 10.2 LHH439 pKa = 6.6 NLIDD443 pKa = 4.46 VPDD446 pKa = 4.36 NLDD449 pKa = 3.38 KK450 pKa = 11.08 PIGYY454 pKa = 9.88 APRR457 pKa = 11.84 YY458 pKa = 8.49 WDD460 pKa = 4.31 FKK462 pKa = 10.29 SCVDD466 pKa = 4.12 KK467 pKa = 11.7 YY468 pKa = 11.16 CGAFNLEE475 pKa = 3.98 QFRR478 pKa = 11.84 GFTIEE483 pKa = 4.51 HH484 pKa = 6.92 PWNLLYY490 pKa = 10.68 DD491 pKa = 4.01 IALSEE496 pKa = 4.38 HH497 pKa = 7.09 PEE499 pKa = 4.32 DD500 pKa = 5.39 LPDD503 pKa = 4.15 IYY505 pKa = 10.98 WMALFKK511 pKa = 10.02 CTPNDD516 pKa = 3.2 AKK518 pKa = 10.91 FIFSALPDD526 pKa = 3.75 STRR529 pKa = 11.84 LTDD532 pKa = 3.52 TFLTSLDD539 pKa = 3.25 FDD541 pKa = 4.57 FKK543 pKa = 11.47 VNRR546 pKa = 11.84 CLSYY550 pKa = 11.35 NGMPYY555 pKa = 10.91
Molecular weight: 63.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.95
IPC2_protein 5.092
IPC_protein 5.092
Toseland 5.13
ProMoST 5.308
Dawson 5.169
Bjellqvist 5.27
Wikipedia 5.105
Rodwell 5.08
Grimsley 5.092
Solomon 5.169
Lehninger 5.13
Nozaki 5.321
DTASelect 5.537
Thurlkill 5.181
EMBOSS 5.207
Sillero 5.385
Patrickios 3.02
IPC_peptide 5.181
IPC2_peptide 5.385
IPC2.peptide.svr19 5.263
Protein with the highest isoelectric point:
>tr|A0A4P8W404|A0A4P8W404_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_82 OX=2584793 PE=3 SV=1
MM1 pKa = 7.78 CIILNPKK8 pKa = 8.0 TQLSEE13 pKa = 4.22 HH14 pKa = 6.42 FSLTEE19 pKa = 3.87 MVSKK23 pKa = 10.85 NDD25 pKa = 3.52 PCHH28 pKa = 7.11 DD29 pKa = 3.85 VLLKK33 pKa = 10.44 KK34 pKa = 10.78 LPVHH38 pKa = 6.67 VYY40 pKa = 9.73 EE41 pKa = 4.49 NLKK44 pKa = 10.1 KK45 pKa = 10.06 VCSILEE51 pKa = 4.18 EE52 pKa = 3.9 FRR54 pKa = 11.84 KK55 pKa = 10.2 ILDD58 pKa = 3.68 CPISVNSALRR68 pKa = 11.84 STSHH72 pKa = 6.65 NIAVGGVTNSYY83 pKa = 9.25 HH84 pKa = 5.65 CHH86 pKa = 5.41 GLAVDD91 pKa = 4.47 FRR93 pKa = 11.84 TNKK96 pKa = 8.68 TALSNFQLLSSAYY109 pKa = 9.34 NLKK112 pKa = 10.21 PYY114 pKa = 10.58 KK115 pKa = 10.29 SGNYY119 pKa = 9.28 LVAKK123 pKa = 9.91 NDD125 pKa = 3.39 KK126 pKa = 10.35 FKK128 pKa = 11.37 LLLYY132 pKa = 7.68 PTFIHH137 pKa = 6.1 MQLQKK142 pKa = 11.18
Molecular weight: 16.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.647
IPC2_protein 8.463
IPC_protein 8.39
Toseland 8.931
ProMoST 9.004
Dawson 9.268
Bjellqvist 9.224
Wikipedia 9.385
Rodwell 9.472
Grimsley 9.18
Solomon 9.385
Lehninger 9.37
Nozaki 9.428
DTASelect 9.077
Thurlkill 9.209
EMBOSS 9.443
Sillero 9.399
Patrickios 4.444
IPC_peptide 9.385
IPC2_peptide 8.039
IPC2.peptide.svr19 7.817
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1734
98
555
346.8
40.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.306 ± 0.595
1.557 ± 0.447
5.536 ± 0.855
5.421 ± 0.811
5.709 ± 1.133
3.922 ± 0.457
2.307 ± 0.636
6.171 ± 0.342
6.92 ± 0.508
10.092 ± 0.763
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.73 ± 0.228
8.939 ± 1.005
4.325 ± 0.732
5.306 ± 1.858
3.633 ± 0.482
6.69 ± 0.467
5.421 ± 0.154
4.268 ± 0.433
0.577 ± 0.213
6.171 ± 1.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here