Salmonella sp. NCTC 7297

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella; unclassified Salmonella

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509D1S3|A0A509D1S3_9ENTR T3SS secreted effector EspN-like protein OS=Salmonella sp. NCTC 7297 OX=2583578 GN=NCTC7297_02951 PE=4 SV=1
MM1 pKa = 7.67LEE3 pKa = 4.0QMEE6 pKa = 4.47LTDD9 pKa = 4.16YY10 pKa = 11.88ANMALSIPSANTNIWNLEE28 pKa = 3.6QDD30 pKa = 3.86TVGTRR35 pKa = 11.84LTNARR40 pKa = 11.84HH41 pKa = 5.4GLADD45 pKa = 4.02NGGAWVSYY53 pKa = 10.43FGGNFNGDD61 pKa = 3.55NGTINYY67 pKa = 8.82DD68 pKa = 3.13QDD70 pKa = 3.61VNGIMVGVDD79 pKa = 2.99TKK81 pKa = 11.4VDD83 pKa = 3.43GNNAKK88 pKa = 9.88WIVGAAAGFAKK99 pKa = 10.49GDD101 pKa = 3.51LSDD104 pKa = 3.46RR105 pKa = 11.84TGQVDD110 pKa = 3.54QDD112 pKa = 3.84SQSAYY117 pKa = 9.05IYY119 pKa = 10.73SSAHH123 pKa = 4.91FANNIFVDD131 pKa = 4.18GNLSYY136 pKa = 10.95FHH138 pKa = 7.14FNNDD142 pKa = 2.59LSANMSDD149 pKa = 3.19GTYY152 pKa = 10.73VDD154 pKa = 5.14GNTSSDD160 pKa = 2.69AWGFGLKK167 pKa = 10.32LGYY170 pKa = 9.47DD171 pKa = 3.67WKK173 pKa = 11.2LGDD176 pKa = 4.4AGYY179 pKa = 7.83LTPYY183 pKa = 10.28GSVSGLFQSGDD194 pKa = 3.79DD195 pKa = 3.89YY196 pKa = 11.83QLSNDD201 pKa = 3.96MKK203 pKa = 11.23VDD205 pKa = 3.62GQSYY209 pKa = 10.64DD210 pKa = 3.02SMRR213 pKa = 11.84YY214 pKa = 8.94EE215 pKa = 4.34LGVDD219 pKa = 3.21AGYY222 pKa = 8.52TFTYY226 pKa = 10.71SDD228 pKa = 4.19DD229 pKa = 3.86QALTPYY235 pKa = 10.29FKK237 pKa = 10.64LAYY240 pKa = 10.52VYY242 pKa = 11.0DD243 pKa = 4.56DD244 pKa = 4.43SNNDD248 pKa = 3.06ADD250 pKa = 4.41VNGDD254 pKa = 3.84SIDD257 pKa = 3.7NGVEE261 pKa = 3.97GSAVRR266 pKa = 11.84VGLGTQFSFTKK277 pKa = 10.49NFSAYY282 pKa = 9.3TDD284 pKa = 3.45ANYY287 pKa = 10.81LGGGDD292 pKa = 4.6VDD294 pKa = 4.32QDD296 pKa = 2.67WSANVGVKK304 pKa = 7.43YY305 pKa = 8.84TWW307 pKa = 3.09

Molecular weight: 33.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509BFQ1|A0A509BFQ1_9ENTR Endolysin OS=Salmonella sp. NCTC 7297 OX=2583578 GN=NCTC7297_00326 PE=4 SV=1
MM1 pKa = 7.4NRR3 pKa = 11.84QSWLLNLSLLKK14 pKa = 8.97THH16 pKa = 6.9PAFRR20 pKa = 11.84AVFLARR26 pKa = 11.84FISIVSLGLLGVAVPVQIQMMTHH49 pKa = 5.51STWQVGLSVTLTGGAMFIGLMVGGVLADD77 pKa = 3.64RR78 pKa = 11.84YY79 pKa = 9.17EE80 pKa = 4.1RR81 pKa = 11.84KK82 pKa = 9.81KK83 pKa = 11.2VILLARR89 pKa = 11.84GTCGVGFIGLCVNALLPEE107 pKa = 4.42PSLLAIYY114 pKa = 10.65LLGLWDD120 pKa = 4.55GFFASLGRR128 pKa = 11.84DD129 pKa = 2.68RR130 pKa = 11.84FYY132 pKa = 10.59WRR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84QRR138 pKa = 11.84WSEE141 pKa = 3.76EE142 pKa = 4.14KK143 pKa = 10.07ICCRR147 pKa = 11.84PARR150 pKa = 11.84LPCC153 pKa = 4.6

Molecular weight: 17.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3497

0

3497

599048

29

1259

171.3

19.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.59 ± 0.051

1.298 ± 0.02

5.085 ± 0.037

5.282 ± 0.045

3.889 ± 0.036

7.312 ± 0.043

2.336 ± 0.028

5.932 ± 0.047

4.255 ± 0.038

10.415 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.027

3.84 ± 0.038

4.533 ± 0.037

4.288 ± 0.039

6.219 ± 0.046

5.968 ± 0.04

5.532 ± 0.042

6.814 ± 0.044

1.573 ± 0.023

2.862 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski