Tomato bushy stunt virus (strain Cherry) (TBSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus; Tomato bushy stunt virus

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P11691|MVP_TBSVC Movement protein OS=Tomato bushy stunt virus (strain Cherry) OX=12147 PE=1 SV=1
MM1 pKa = 7.28EE2 pKa = 5.41RR3 pKa = 11.84AIQGNDD9 pKa = 2.68ARR11 pKa = 11.84EE12 pKa = 3.99QANSEE17 pKa = 4.02RR18 pKa = 11.84WDD20 pKa = 3.81GGSGGTTSPFKK31 pKa = 11.1LPDD34 pKa = 3.7EE35 pKa = 4.69SPSWTEE41 pKa = 3.35WRR43 pKa = 11.84LHH45 pKa = 5.57NDD47 pKa = 3.33EE48 pKa = 4.85TNSNQDD54 pKa = 2.84NPLGFKK60 pKa = 10.19EE61 pKa = 3.92SWGFGKK67 pKa = 10.5VVFKK71 pKa = 10.8RR72 pKa = 11.84YY73 pKa = 9.61LRR75 pKa = 11.84YY76 pKa = 10.37DD77 pKa = 3.21RR78 pKa = 11.84TEE80 pKa = 3.87ASLHH84 pKa = 5.75RR85 pKa = 11.84VLGSWTGDD93 pKa = 3.38SVNYY97 pKa = 9.27AASRR101 pKa = 11.84FFGFDD106 pKa = 3.33QIGCTYY112 pKa = 10.29SIRR115 pKa = 11.84FRR117 pKa = 11.84GVSITVSGGSRR128 pKa = 11.84TLQHH132 pKa = 6.31LCEE135 pKa = 3.73MAIRR139 pKa = 11.84SKK141 pKa = 11.13QEE143 pKa = 3.85LLQLAPIEE151 pKa = 4.34VEE153 pKa = 4.45SNVSRR158 pKa = 11.84GCPEE162 pKa = 3.6GTEE165 pKa = 4.22TFEE168 pKa = 5.11KK169 pKa = 10.56EE170 pKa = 4.08SEE172 pKa = 4.07

Molecular weight: 19.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15962|RDRP_TBSVC RNA-directed RNA polymerase OS=Tomato bushy stunt virus (strain Cherry) OX=12147 GN=ORF1 PE=3 SV=2
MM1 pKa = 7.26DD2 pKa = 3.77TEE4 pKa = 4.47YY5 pKa = 11.2EE6 pKa = 4.24QVNKK10 pKa = 9.64PWNEE14 pKa = 3.86LYY16 pKa = 10.75KK17 pKa = 10.36EE18 pKa = 4.31TTLGNKK24 pKa = 8.22LTVNVGMEE32 pKa = 4.07DD33 pKa = 3.42QEE35 pKa = 4.95VPLLPSNFLTKK46 pKa = 10.35VRR48 pKa = 11.84VGLSGGYY55 pKa = 7.7ITMRR59 pKa = 11.84RR60 pKa = 11.84IRR62 pKa = 11.84IKK64 pKa = 10.3IIPLVSRR71 pKa = 11.84KK72 pKa = 10.22AGVSGKK78 pKa = 10.6LYY80 pKa = 10.76LRR82 pKa = 11.84DD83 pKa = 3.52ISDD86 pKa = 3.28TTGRR90 pKa = 11.84KK91 pKa = 8.28LHH93 pKa = 6.02CTEE96 pKa = 4.24SLDD99 pKa = 3.5LGRR102 pKa = 11.84EE103 pKa = 3.65IRR105 pKa = 11.84LTMQHH110 pKa = 6.81LDD112 pKa = 3.56FSVSTRR118 pKa = 11.84SDD120 pKa = 3.36VPIVFGFEE128 pKa = 3.92EE129 pKa = 4.62LVSPFLEE136 pKa = 4.29GRR138 pKa = 11.84EE139 pKa = 4.0LFSISVRR146 pKa = 11.84WQFGLSKK153 pKa = 10.58NCYY156 pKa = 9.96SLPQSKK162 pKa = 9.04WKK164 pKa = 10.7VMYY167 pKa = 10.56QEE169 pKa = 5.23DD170 pKa = 4.07ALKK173 pKa = 10.67VLRR176 pKa = 11.84PSKK179 pKa = 10.54KK180 pKa = 9.65KK181 pKa = 10.59ASKK184 pKa = 9.5TDD186 pKa = 3.22SSVV189 pKa = 2.62

Molecular weight: 21.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1567

172

818

391.8

43.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.892 ± 1.117

1.914 ± 0.337

4.595 ± 0.152

5.68 ± 1.102

3.957 ± 0.212

8.041 ± 0.685

1.851 ± 0.3

4.467 ± 0.152

5.424 ± 0.814

9.19 ± 0.545

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.215

4.084 ± 0.66

4.34 ± 0.377

3.063 ± 0.207

7.403 ± 0.855

6.892 ± 1.243

6.701 ± 1.581

8.743 ± 0.685

1.34 ± 0.298

3.127 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski