Anas platyrhynchos platyrhynchos (Northern mallard)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27053 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U3I454|U3I454_ANAPP Ubiquitin specific peptidase 1 OS=Anas platyrhynchos platyrhynchos OX=8840 GN=USP1 PE=4 SV=2
MM1 pKa = 7.46 FDD3 pKa = 3.56 CVASEE8 pKa = 4.1 FDD10 pKa = 3.41 IDD12 pKa = 3.73 PARR15 pKa = 11.84 TIMVGDD21 pKa = 4.1 RR22 pKa = 11.84 LDD24 pKa = 3.49 TDD26 pKa = 3.12 ILMGNTCGLTTLLTLTGVTTLEE48 pKa = 3.95 EE49 pKa = 4.41 VKK51 pKa = 10.61 GHH53 pKa = 6.09 EE54 pKa = 4.72 EE55 pKa = 3.8 SDD57 pKa = 3.78 CPARR61 pKa = 11.84 QSLVPDD67 pKa = 3.84 YY68 pKa = 10.55 YY69 pKa = 11.09 VDD71 pKa = 4.38 SIADD75 pKa = 3.81 ILPALQDD82 pKa = 3.29
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.465
ProMoST 3.808
Dawson 3.694
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.63
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.808
Patrickios 1.875
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A493THG4|A0A493THG4_ANAPP Glycine N-acyltransferase-like protein OS=Anas platyrhynchos platyrhynchos OX=8840 GN=GLYATL3 PE=3 SV=1
MM1 pKa = 7.55 GFFGQDD7 pKa = 3.14 FSVPWGRR14 pKa = 11.84 SVPWGHH20 pKa = 6.99 PKK22 pKa = 9.28 TRR24 pKa = 11.84 GHH26 pKa = 6.84 PKK28 pKa = 10.45 AGGHH32 pKa = 5.65 PKK34 pKa = 10.28 SRR36 pKa = 11.84 GHH38 pKa = 6.57 PKK40 pKa = 9.29 TRR42 pKa = 11.84 GHH44 pKa = 6.74 PKK46 pKa = 8.79 TRR48 pKa = 11.84 RR49 pKa = 11.84 HH50 pKa = 5.89 PKK52 pKa = 8.87 TRR54 pKa = 11.84 GHH56 pKa = 6.81 PKK58 pKa = 10.5 AGGHH62 pKa = 5.7 PKK64 pKa = 10.52 ARR66 pKa = 11.84 GHH68 pKa = 6.48 PKK70 pKa = 10.48 AGGHH74 pKa = 5.62 PKK76 pKa = 10.03 PRR78 pKa = 11.84 GHH80 pKa = 7.09 PKK82 pKa = 9.31 TRR84 pKa = 11.84 GHH86 pKa = 6.62 PNARR90 pKa = 11.84 GHH92 pKa = 6.47 PKK94 pKa = 10.56 AGGHH98 pKa = 6.13 PKK100 pKa = 9.3 TRR102 pKa = 11.84 GHH104 pKa = 6.8 PKK106 pKa = 8.63 TRR108 pKa = 11.84 GYY110 pKa = 9.25 PKK112 pKa = 10.24 PRR114 pKa = 11.84 GHH116 pKa = 6.75 PKK118 pKa = 10.26 SRR120 pKa = 11.84 GHH122 pKa = 6.52 PKK124 pKa = 10.45 SGGHH128 pKa = 6.31 PKK130 pKa = 9.3 TRR132 pKa = 11.84 GHH134 pKa = 6.94 PNTRR138 pKa = 11.84 GHH140 pKa = 6.66 PKK142 pKa = 9.99 PRR144 pKa = 11.84 GHH146 pKa = 6.81 PKK148 pKa = 10.49 ARR150 pKa = 11.84 GHH152 pKa = 6.48 PKK154 pKa = 10.48 AGGHH158 pKa = 5.62 PKK160 pKa = 10.07 PRR162 pKa = 11.84 GHH164 pKa = 6.81 PKK166 pKa = 10.46 ARR168 pKa = 11.84 GHH170 pKa = 6.44 PKK172 pKa = 9.27 TRR174 pKa = 11.84 GHH176 pKa = 6.81 PKK178 pKa = 9.23 TRR180 pKa = 11.84 GHH182 pKa = 6.73 PKK184 pKa = 10.38 SGGHH188 pKa = 5.86 PKK190 pKa = 10.51 ARR192 pKa = 11.84 GHH194 pKa = 6.39 PKK196 pKa = 10.41 SGGHH200 pKa = 6.31 PKK202 pKa = 9.31 TRR204 pKa = 11.84 GHH206 pKa = 6.73 PKK208 pKa = 10.38 SGGHH212 pKa = 5.86 PKK214 pKa = 10.5 ARR216 pKa = 11.84 GHH218 pKa = 6.44 PKK220 pKa = 9.27 TRR222 pKa = 11.84 GHH224 pKa = 6.73 PKK226 pKa = 10.34 SGGHH230 pKa = 5.85 PKK232 pKa = 10.31 SRR234 pKa = 11.84 GHH236 pKa = 6.44 PKK238 pKa = 10.06 SGRR241 pKa = 11.84 HH242 pKa = 5.01 PKK244 pKa = 10.15 SRR246 pKa = 11.84 GHH248 pKa = 6.23 PKK250 pKa = 10.0 PRR252 pKa = 11.84 GHH254 pKa = 7.07 PKK256 pKa = 8.84 TRR258 pKa = 11.84 RR259 pKa = 11.84 HH260 pKa = 6.22 PKK262 pKa = 9.49 TGGHH266 pKa = 6.4 PKK268 pKa = 10.2 NRR270 pKa = 11.84 GHH272 pKa = 7.06 PKK274 pKa = 9.24 TRR276 pKa = 11.84 GHH278 pKa = 6.61 PKK280 pKa = 9.91 PRR282 pKa = 11.84 GHH284 pKa = 7.09 PKK286 pKa = 9.29 TRR288 pKa = 11.84 GHH290 pKa = 6.74 PKK292 pKa = 8.79 TRR294 pKa = 11.84 RR295 pKa = 11.84 HH296 pKa = 5.62 PKK298 pKa = 9.35 PRR300 pKa = 11.84 GHH302 pKa = 6.39 RR303 pKa = 11.84 KK304 pKa = 9.36 SRR306 pKa = 11.84 GHH308 pKa = 6.56 PKK310 pKa = 9.3 TRR312 pKa = 11.84 GHH314 pKa = 6.81 PKK316 pKa = 9.26 TRR318 pKa = 11.84 GHH320 pKa = 6.84 PKK322 pKa = 10.47 AGGHH326 pKa = 6.54 PKK328 pKa = 9.98 TGGHH332 pKa = 6.73 PKK334 pKa = 8.96 TRR336 pKa = 11.84 APPVPRR342 pKa = 11.84 HH343 pKa = 5.43 RR344 pKa = 11.84 SIPRR348 pKa = 11.84 ALPVPRR354 pKa = 11.84 PCPGAPWGPPGAAAAAGPGPPLTTPGISRR383 pKa = 11.84 SFAPCSPGSRR393 pKa = 11.84 RR394 pKa = 11.84 RR395 pKa = 11.84 RR396 pKa = 11.84 RR397 pKa = 11.84 RR398 pKa = 11.84 NN399 pKa = 2.95
Molecular weight: 42.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.33
IPC_protein 12.852
Toseland 13.027
ProMoST 13.51
Dawson 13.027
Bjellqvist 13.013
Wikipedia 13.495
Rodwell 12.808
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.027
DTASelect 13.013
Thurlkill 13.027
EMBOSS 13.525
Sillero 13.027
Patrickios 12.515
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.175
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16599
10454
27053
16497361
36
6232
609.8
67.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.978 ± 0.013
2.265 ± 0.011
4.866 ± 0.009
7.149 ± 0.019
3.577 ± 0.01
6.428 ± 0.021
2.522 ± 0.006
4.509 ± 0.011
6.021 ± 0.017
9.53 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.006
3.905 ± 0.009
5.955 ± 0.02
4.741 ± 0.012
5.579 ± 0.013
8.471 ± 0.02
5.319 ± 0.011
6.074 ± 0.011
1.193 ± 0.005
2.702 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here