Rubellimicrobium mesophilum DSM 19309

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Rubellimicrobium; Rubellimicrobium mesophilum

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A017HIU9|A0A017HIU9_9RHOB GTA NlpC/P60 family peptidase OS=Rubellimicrobium mesophilum DSM 19309 OX=442562 GN=Rumeso_03964 PE=3 SV=1
MM1 pKa = 7.3TRR3 pKa = 11.84LTTATALALIWASGALAQTEE23 pKa = 4.3LTMWYY28 pKa = 9.48HH29 pKa = 5.91GAGNQVEE36 pKa = 4.48SEE38 pKa = 4.66IINQIISDD46 pKa = 4.13FNASQSDD53 pKa = 3.24WHH55 pKa = 5.47VTIEE59 pKa = 4.34SFPQAAYY66 pKa = 10.8NDD68 pKa = 4.13AVVAAALAGDD78 pKa = 4.59LPDD81 pKa = 4.6ILDD84 pKa = 3.42VDD86 pKa = 4.67GPIMPNWAWAGYY98 pKa = 7.77LQPLPIDD105 pKa = 3.61PAKK108 pKa = 10.48IEE110 pKa = 4.25SFLPGPKK117 pKa = 9.13GTWNDD122 pKa = 3.15QLYY125 pKa = 10.91SVGLWDD131 pKa = 4.01AAVALVTRR139 pKa = 11.84QSTLDD144 pKa = 3.53EE145 pKa = 4.93LGLRR149 pKa = 11.84TPTLEE154 pKa = 4.19EE155 pKa = 4.0PWTLEE160 pKa = 3.76EE161 pKa = 5.23FMGAMEE167 pKa = 4.41AAKK170 pKa = 10.64ASGKK174 pKa = 10.05YY175 pKa = 10.04DD176 pKa = 3.5YY177 pKa = 11.18AIDD180 pKa = 5.27LGMSDD185 pKa = 3.01QGEE188 pKa = 4.35WYY190 pKa = 9.03PYY192 pKa = 11.22AFLPFLWSFGGDD204 pKa = 3.37LVDD207 pKa = 4.5VQADD211 pKa = 3.6PQTAEE216 pKa = 4.01GVLNGDD222 pKa = 3.69AGLKK226 pKa = 9.89FGEE229 pKa = 4.02WWQGMFANGYY239 pKa = 10.37APGPSQDD246 pKa = 4.13PADD249 pKa = 4.04HH250 pKa = 7.12ASGFQEE256 pKa = 3.99GKK258 pKa = 10.64YY259 pKa = 10.04AFSWNGNWLALPFLEE274 pKa = 6.02AYY276 pKa = 10.29DD277 pKa = 4.14DD278 pKa = 4.38VVFLPAPDD286 pKa = 4.4FGNGNYY292 pKa = 9.6IGGASWQFAVSGTTEE307 pKa = 3.99HH308 pKa = 7.35PEE310 pKa = 3.73GGAAFIEE317 pKa = 4.86FALQDD322 pKa = 3.24KK323 pKa = 10.5YY324 pKa = 11.16LAQFSDD330 pKa = 5.05GIGLIPPTTTAAAMTQNYY348 pKa = 9.13KK349 pKa = 10.57EE350 pKa = 4.35SGPMAVFYY358 pKa = 10.81DD359 pKa = 4.38LSADD363 pKa = 3.67QARR366 pKa = 11.84LRR368 pKa = 11.84PVTPGYY374 pKa = 9.65PVASKK379 pKa = 11.12VFLKK383 pKa = 11.21AMMDD387 pKa = 3.45IANGADD393 pKa = 3.34VADD396 pKa = 4.32TLDD399 pKa = 3.68SAVDD403 pKa = 4.27EE404 pKa = 4.58IDD406 pKa = 5.5ADD408 pKa = 3.53IQANNGYY415 pKa = 9.69AQAQQ419 pKa = 3.46

Molecular weight: 45.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A017HQN9|A0A017HQN9_9RHOB Recombinase OS=Rubellimicrobium mesophilum DSM 19309 OX=442562 GN=Rumeso_02468 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84AVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.41VLCAA44 pKa = 3.88

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4946

0

4946

1389346

37

2011

280.9

30.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.996 ± 0.051

0.818 ± 0.011

5.774 ± 0.033

6.122 ± 0.035

3.29 ± 0.02

9.43 ± 0.047

2.095 ± 0.018

4.135 ± 0.026

2.2 ± 0.025

10.56 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.018

2.084 ± 0.018

5.83 ± 0.033

2.99 ± 0.023

8.19 ± 0.044

4.881 ± 0.027

5.194 ± 0.028

7.527 ± 0.026

1.563 ± 0.016

1.916 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski