Carnation yellow fleck virus
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5IV73|V5IV73_9CLOS HSP70 OS=Carnation yellow fleck virus OX=940280 PE=3 SV=1
MM1 pKa = 7.19 NAVEE5 pKa = 4.5 FAQPKK10 pKa = 9.73 EE11 pKa = 3.93 FFKK14 pKa = 10.96 ILLLKK19 pKa = 10.88 DD20 pKa = 3.31 FVFYY24 pKa = 10.44 IVSIDD29 pKa = 3.52 CHH31 pKa = 7.44 AEE33 pKa = 3.91 STAEE37 pKa = 3.95 LAVVYY42 pKa = 10.2 LQDD45 pKa = 4.5 FDD47 pKa = 4.34 LTFNRR52 pKa = 11.84 KK53 pKa = 8.5 GQVSSEE59 pKa = 3.95 PFDD62 pKa = 3.84 FEE64 pKa = 5.68 NIVSKK69 pKa = 10.91 LNPKK73 pKa = 8.47 TLTYY77 pKa = 10.14 TRR79 pKa = 11.84 VRR81 pKa = 11.84 SGDD84 pKa = 3.61 TVGSQPVHH92 pKa = 6.79 ALDD95 pKa = 4.0 SDD97 pKa = 3.86 SRR99 pKa = 11.84 LLNRR103 pKa = 11.84 EE104 pKa = 3.81 VNFIFHH110 pKa = 6.35 QNVKK114 pKa = 8.96 LTFGLYY120 pKa = 10.54 GEE122 pKa = 4.45 EE123 pKa = 4.39 QYY125 pKa = 11.08 CVSSDD130 pKa = 3.39 YY131 pKa = 11.39 LQFGSKK137 pKa = 10.45 FIGAHH142 pKa = 6.3 CGTLSYY148 pKa = 11.07 CLLRR152 pKa = 11.84 EE153 pKa = 4.1 LDD155 pKa = 3.81 SDD157 pKa = 3.96 NKK159 pKa = 10.44 NLVVGTFCAA168 pKa = 3.94
Molecular weight: 19.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.95
IPC2_protein 5.105
IPC_protein 5.003
Toseland 4.965
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.143
Wikipedia 4.914
Rodwell 4.927
Grimsley 4.889
Solomon 5.016
Lehninger 4.978
Nozaki 5.156
DTASelect 5.321
Thurlkill 4.991
EMBOSS 4.965
Sillero 5.219
Patrickios 3.541
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.227
Protein with the highest isoelectric point:
>tr|V5IV68|V5IV68_9CLOS p17 OS=Carnation yellow fleck virus OX=940280 PE=4 SV=1
MM1 pKa = 7.79 DD2 pKa = 4.88 CVLRR6 pKa = 11.84 SYY8 pKa = 11.44 LLLAFGFLICLFLFCLVVFVWFVYY32 pKa = 10.53 RR33 pKa = 11.84 NVLSVTPQSPTNTQRR48 pKa = 11.84 TAVVV52 pKa = 3.13
Molecular weight: 6.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.919
IPC2_protein 7.614
IPC_protein 7.819
Toseland 7.146
ProMoST 8.083
Dawson 8.258
Bjellqvist 8.653
Wikipedia 8.185
Rodwell 8.229
Grimsley 7.0
Solomon 8.521
Lehninger 8.551
Nozaki 8.96
DTASelect 8.361
Thurlkill 8.39
EMBOSS 8.507
Sillero 8.77
Patrickios 0.604
IPC_peptide 8.507
IPC2_peptide 8.785
IPC2.peptide.svr19 8.976
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
5119
52
2664
568.8
64.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.193 ± 0.381
2.325 ± 0.226
4.981 ± 0.289
6.017 ± 0.433
6.505 ± 0.51
4.923 ± 0.421
1.66 ± 0.234
4.2 ± 0.266
5.919 ± 0.381
11.037 ± 0.325
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.231 ± 0.292
4.708 ± 0.518
3.79 ± 0.351
2.032 ± 0.232
6.388 ± 0.672
10.334 ± 0.723
5.821 ± 0.351
7.775 ± 0.6
0.508 ± 0.11
3.653 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here