Nitratireductor sp. StC3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Nitratireductor; unclassified Nitratireductor

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8NED5|A0A2P8NED5_9RHIZ ZIP family metal transporter OS=Nitratireductor sp. StC3 OX=2126741 GN=C7T96_09430 PE=4 SV=1
DDD2 pKa = 4.33GNGFFAAISLEEE14 pKa = 3.93DD15 pKa = 4.05DD16 pKa = 3.66LSGEEE21 pKa = 4.76YYY23 pKa = 9.42PDDD26 pKa = 3.22VAKKK30 pKa = 10.5GISQGWGAVWAKKK43 pKa = 10.05AYYY46 pKa = 9.97DD47 pKa = 3.59EE48 pKa = 5.56FDDD51 pKa = 4.38STGTLNALTGTVNSGGFGAQLGAQINIPNMPGSSLRR87 pKa = 11.84LMASYYY93 pKa = 11.5DDD95 pKa = 3.87SHHH98 pKa = 6.6FNVGAPTINVAAGITGLPPFIPGVSLYYY126 pKa = 11.04GGLFGGSEEE135 pKa = 3.98SILASYYY142 pKa = 9.57HHH144 pKa = 6.0FSSTLGASVAFQWFNDDD161 pKa = 3.82YYY163 pKa = 11.43AGTDDD168 pKa = 3.22KKK170 pKa = 10.29GVDDD174 pKa = 2.64WGAEEE179 pKa = 4.26SLVWFPVSDDD189 pKa = 3.78EEE191 pKa = 4.92RR192 pKa = 11.84TEEE195 pKa = 3.48HHH197 pKa = 6.52YY198 pKa = 11.19DD199 pKa = 3.45DD200 pKa = 4.58GSIGGFNPDDD210 pKa = 3.37TVSGYYY216 pKa = 11.0RR217 pKa = 11.84FTRR220 pKa = 11.84YYY222 pKa = 7.57

Molecular weight: 23.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8NAL2|A0A2P8NAL2_9RHIZ Methylhydantoinase OS=Nitratireductor sp. StC3 OX=2126741 GN=C7T96_18910 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.99GGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84SRR41 pKa = 11.84LSAA44 pKa = 3.83

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4621

0

4621

1462021

29

2938

316.4

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.365 ± 0.053

0.847 ± 0.011

5.89 ± 0.027

5.776 ± 0.032

3.853 ± 0.026

8.787 ± 0.031

2.022 ± 0.016

5.066 ± 0.026

3.002 ± 0.03

9.969 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.017

2.374 ± 0.018

5.093 ± 0.028

2.775 ± 0.019

7.524 ± 0.039

4.95 ± 0.02

5.121 ± 0.022

7.59 ± 0.03

1.303 ± 0.013

2.205 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski