Nitratireductor sp. StC3
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4621 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P8NED5|A0A2P8NED5_9RHIZ ZIP family metal transporter OS=Nitratireductor sp. StC3 OX=2126741 GN=C7T96_09430 PE=4 SV=1
DDD2 pKa = 4.33 GNGFFAAISLEEE14 pKa = 3.93 DD15 pKa = 4.05 DD16 pKa = 3.66 LSGEEE21 pKa = 4.76 YYY23 pKa = 9.42 PDDD26 pKa = 3.22 VAKKK30 pKa = 10.5 GISQGWGAVWAKKK43 pKa = 10.05 AYYY46 pKa = 9.97 DD47 pKa = 3.59 EE48 pKa = 5.56 FDDD51 pKa = 4.38 STGTLNALTGTVNSGGFGAQLGAQINIPNMPGSSLRR87 pKa = 11.84 LMASYYY93 pKa = 11.5 DDD95 pKa = 3.87 SHHH98 pKa = 6.6 FNVGAPTINVAAGITGLPPFIPGVSLYYY126 pKa = 11.04 GGLFGGSEEE135 pKa = 3.98 SILASYYY142 pKa = 9.57 HHH144 pKa = 6.0 FSSTLGASVAFQWFNDDD161 pKa = 3.82 YYY163 pKa = 11.43 AGTDDD168 pKa = 3.22 KKK170 pKa = 10.29 GVDDD174 pKa = 2.64 WGAEEE179 pKa = 4.26 SLVWFPVSDDD189 pKa = 3.78 EEE191 pKa = 4.92 RR192 pKa = 11.84 TEEE195 pKa = 3.48 HHH197 pKa = 6.52 YY198 pKa = 11.19 DD199 pKa = 3.45 DD200 pKa = 4.58 GSIGGFNPDDD210 pKa = 3.37 TVSGYYY216 pKa = 11.0 RR217 pKa = 11.84 FTRR220 pKa = 11.84 YYY222 pKa = 7.57
Molecular weight: 23.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 4.037
IPC_protein 3.999
Toseland 3.795
ProMoST 4.202
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.935
Rodwell 3.834
Grimsley 3.706
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.355
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.126
Patrickios 1.036
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.0
Protein with the highest isoelectric point:
>tr|A0A2P8NAL2|A0A2P8NAL2_9RHIZ Methylhydantoinase OS=Nitratireductor sp. StC3 OX=2126741 GN=C7T96_18910 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 9.99 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 NRR37 pKa = 11.84 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LSAA44 pKa = 3.83
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4621
0
4621
1462021
29
2938
316.4
34.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.365 ± 0.053
0.847 ± 0.011
5.89 ± 0.027
5.776 ± 0.032
3.853 ± 0.026
8.787 ± 0.031
2.022 ± 0.016
5.066 ± 0.026
3.002 ± 0.03
9.969 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.487 ± 0.017
2.374 ± 0.018
5.093 ± 0.028
2.775 ± 0.019
7.524 ± 0.039
4.95 ± 0.02
5.121 ± 0.022
7.59 ± 0.03
1.303 ± 0.013
2.205 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here