Amino acid dipepetide frequency for Nitratireductor sp. StC3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.449AlaAla: 19.449 ± 0.185	1.189AlaCys: 1.189 ± 0.032	7.614AlaAsp: 7.614 ± 0.074	8.336AlaGlu: 8.336 ± 0.093	4.942AlaPhe: 4.942 ± 0.058	12.557AlaGly: 12.557 ± 0.104	2.371AlaHis: 2.371 ± 0.038	6.814AlaIle: 6.814 ± 0.077	3.495AlaLys: 3.495 ± 0.06	14.507AlaLeu: 14.507 ± 0.12	3.726AlaMet: 3.726 ± 0.05	2.719AlaAsn: 2.719 ± 0.041	5.834AlaPro: 5.834 ± 0.087	3.492AlaGln: 3.492 ± 0.057	10.413AlaArg: 10.413 ± 0.101	6.0AlaSer: 6.0 ± 0.065	6.107AlaThr: 6.107 ± 0.063	9.653AlaVal: 9.653 ± 0.095	1.642AlaTrp: 1.642 ± 0.039	2.792AlaTyr: 2.792 ± 0.046	0.001AlaXaa: 0.001 ± 0.001
Cys	1.066CysAla: 1.066 ± 0.029	0.097CysCys: 0.097 ± 0.008	0.543CysAsp: 0.543 ± 0.02	0.455CysGlu: 0.455 ± 0.018	0.344CysPhe: 0.344 ± 0.016	0.96CysGly: 0.96 ± 0.032	0.261CysHis: 0.261 ± 0.014	0.319CysIle: 0.319 ± 0.015	0.167CysLys: 0.167 ± 0.011	0.811CysLeu: 0.811 ± 0.024	0.148CysMet: 0.148 ± 0.011	0.172CysAsn: 0.172 ± 0.011	0.446CysPro: 0.446 ± 0.018	0.22CysGln: 0.22 ± 0.014	0.69CysArg: 0.69 ± 0.026	0.394CysSer: 0.394 ± 0.015	0.407CysThr: 0.407 ± 0.017	0.648CysVal: 0.648 ± 0.023	0.113CysTrp: 0.113 ± 0.008	0.207CysTyr: 0.207 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.328AspAla: 7.328 ± 0.085	0.495AspCys: 0.495 ± 0.023	3.459AspAsp: 3.459 ± 0.061	3.67AspGlu: 3.67 ± 0.062	2.284AspPhe: 2.284 ± 0.042	5.798AspGly: 5.798 ± 0.069	1.268AspHis: 1.268 ± 0.03	3.292AspIle: 3.292 ± 0.049	1.752AspLys: 1.752 ± 0.041	5.718AspLeu: 5.718 ± 0.066	1.511AspMet: 1.511 ± 0.03	1.297AspAsn: 1.297 ± 0.034	3.569AspPro: 3.569 ± 0.053	1.568AspGln: 1.568 ± 0.036	4.738AspArg: 4.738 ± 0.065	1.888AspSer: 1.888 ± 0.036	2.78AspThr: 2.78 ± 0.043	3.908AspVal: 3.908 ± 0.055	0.985AspTrp: 0.985 ± 0.023	1.59AspTyr: 1.59 ± 0.039	0.001AspXaa: 0.001 ± 0.001
Glu	8.479GluAla: 8.479 ± 0.082	0.35GluCys: 0.35 ± 0.015	2.834GluAsp: 2.834 ± 0.051	3.185GluGlu: 3.185 ± 0.05	1.776GluPhe: 1.776 ± 0.035	4.041GluGly: 4.041 ± 0.06	1.137GluHis: 1.137 ± 0.033	3.823GluIle: 3.823 ± 0.047	2.298GluLys: 2.298 ± 0.046	5.129GluLeu: 5.129 ± 0.058	1.47GluMet: 1.47 ± 0.033	1.682GluAsn: 1.682 ± 0.039	2.963GluPro: 2.963 ± 0.053	1.907GluGln: 1.907 ± 0.035	5.492GluArg: 5.492 ± 0.075	2.117GluSer: 2.117 ± 0.039	3.992GluThr: 3.992 ± 0.058	3.557GluVal: 3.557 ± 0.043	0.635GluTrp: 0.635 ± 0.022	0.894GluTyr: 0.894 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	5.129PheAla: 5.129 ± 0.055	0.438PheCys: 0.438 ± 0.017	2.812PheAsp: 2.812 ± 0.043	2.278PheGlu: 2.278 ± 0.034	1.631PhePhe: 1.631 ± 0.039	3.884PheGly: 3.884 ± 0.052	0.73PheHis: 0.73 ± 0.023	1.573PheIle: 1.573 ± 0.034	0.893PheLys: 0.893 ± 0.028	3.518PheLeu: 3.518 ± 0.054	0.81PheMet: 0.81 ± 0.024	0.973PheAsn: 0.973 ± 0.027	1.584PhePro: 1.584 ± 0.035	0.938PheGln: 0.938 ± 0.025	2.41PheArg: 2.41 ± 0.043	2.352PheSer: 2.352 ± 0.043	1.819PheThr: 1.819 ± 0.037	3.243PheVal: 3.243 ± 0.048	0.594PheTrp: 0.594 ± 0.02	0.925PheTyr: 0.925 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.414GlyAla: 10.414 ± 0.106	0.846GlyCys: 0.846 ± 0.025	4.626GlyAsp: 4.626 ± 0.063	5.218GlyGlu: 5.218 ± 0.065	3.869GlyPhe: 3.869 ± 0.051	7.55GlyGly: 7.55 ± 0.084	1.979GlyHis: 1.979 ± 0.032	4.579GlyIle: 4.579 ± 0.07	2.98GlyLys: 2.98 ± 0.047	9.377GlyLeu: 9.377 ± 0.083	2.466GlyMet: 2.466 ± 0.044	1.999GlyAsn: 1.999 ± 0.039	3.669GlyPro: 3.669 ± 0.052	2.564GlyGln: 2.564 ± 0.047	6.925GlyArg: 6.925 ± 0.079	4.319GlySer: 4.319 ± 0.055	4.583GlyThr: 4.583 ± 0.068	6.205GlyVal: 6.205 ± 0.066	1.352GlyTrp: 1.352 ± 0.029	2.354GlyTyr: 2.354 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.486HisAla: 2.486 ± 0.046	0.259HisCys: 0.259 ± 0.015	1.304HisAsp: 1.304 ± 0.034	1.065HisGlu: 1.065 ± 0.025	0.865HisPhe: 0.865 ± 0.026	2.033HisGly: 2.033 ± 0.043	0.52HisHis: 0.52 ± 0.021	0.897HisIle: 0.897 ± 0.022	0.458HisLys: 0.458 ± 0.02	1.992HisLeu: 1.992 ± 0.04	0.475HisMet: 0.475 ± 0.019	0.425HisAsn: 0.425 ± 0.018	1.268HisPro: 1.268 ± 0.03	0.535HisGln: 0.535 ± 0.018	1.461HisArg: 1.461 ± 0.033	0.876HisSer: 0.876 ± 0.027	0.855HisThr: 0.855 ± 0.023	1.604HisVal: 1.604 ± 0.032	0.287HisTrp: 0.287 ± 0.016	0.551HisTyr: 0.551 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.917IleAla: 7.917 ± 0.081	0.48IleCys: 0.48 ± 0.02	3.585IleAsp: 3.585 ± 0.054	3.554IleGlu: 3.554 ± 0.049	1.754IlePhe: 1.754 ± 0.036	5.017IleGly: 5.017 ± 0.059	0.874IleHis: 0.874 ± 0.024	1.995IleIle: 1.995 ± 0.039	1.228IleLys: 1.228 ± 0.032	4.326IleLeu: 4.326 ± 0.065	0.977IleMet: 0.977 ± 0.026	1.226IleAsn: 1.226 ± 0.032	2.13IlePro: 2.13 ± 0.041	1.001IleGln: 1.001 ± 0.028	3.2IleArg: 3.2 ± 0.051	2.518IleSer: 2.518 ± 0.05	2.36IleThr: 2.36 ± 0.041	4.869IleVal: 4.869 ± 0.063	0.549IleTrp: 0.549 ± 0.02	1.096IleTyr: 1.096 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.02LysAla: 4.02 ± 0.059	0.154LysCys: 0.154 ± 0.012	1.559LysAsp: 1.559 ± 0.033	1.407LysGlu: 1.407 ± 0.03	0.756LysPhe: 0.756 ± 0.028	2.389LysGly: 2.389 ± 0.044	0.559LysHis: 0.559 ± 0.019	1.577LysIle: 1.577 ± 0.033	1.337LysLys: 1.337 ± 0.037	2.834LysLeu: 2.834 ± 0.046	0.698LysMet: 0.698 ± 0.026	0.817LysAsn: 0.817 ± 0.027	2.001LysPro: 2.001 ± 0.04	0.935LysGln: 0.935 ± 0.028	2.297LysArg: 2.297 ± 0.04	1.68LysSer: 1.68 ± 0.033	1.966LysThr: 1.966 ± 0.04	2.127LysVal: 2.127 ± 0.036	0.35LysTrp: 0.35 ± 0.016	0.557LysTyr: 0.557 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	14.736LeuAla: 14.736 ± 0.119	0.888LeuCys: 0.888 ± 0.029	6.318LeuAsp: 6.318 ± 0.077	5.356LeuGlu: 5.356 ± 0.061	3.893LeuPhe: 3.893 ± 0.059	8.204LeuGly: 8.204 ± 0.083	1.804LeuHis: 1.804 ± 0.039	4.777LeuIle: 4.777 ± 0.07	3.308LeuLys: 3.308 ± 0.051	9.044LeuLeu: 9.044 ± 0.105	2.262LeuMet: 2.262 ± 0.04	2.28LeuAsn: 2.28 ± 0.038	5.219LeuPro: 5.219 ± 0.059	2.339LeuGln: 2.339 ± 0.046	6.654LeuArg: 6.654 ± 0.067	6.184LeuSer: 6.184 ± 0.066	5.115LeuThr: 5.115 ± 0.066	8.085LeuVal: 8.085 ± 0.084	1.129LeuTrp: 1.129 ± 0.028	2.095LeuTyr: 2.095 ± 0.036	0.001LeuXaa: 0.001 ± 0.001
Met	3.274MetAla: 3.274 ± 0.044	0.162MetCys: 0.162 ± 0.011	1.086MetAsp: 1.086 ± 0.027	1.129MetGlu: 1.129 ± 0.03	0.695MetPhe: 0.695 ± 0.021	1.771MetGly: 1.771 ± 0.035	0.486MetHis: 0.486 ± 0.019	1.436MetIle: 1.436 ± 0.034	0.95MetLys: 0.95 ± 0.026	2.49MetLeu: 2.49 ± 0.047	0.687MetMet: 0.687 ± 0.024	0.785MetAsn: 0.785 ± 0.023	1.466MetPro: 1.466 ± 0.033	0.759MetGln: 0.759 ± 0.026	2.01MetArg: 2.01 ± 0.036	1.606MetSer: 1.606 ± 0.034	1.769MetThr: 1.769 ± 0.034	1.813MetVal: 1.813 ± 0.037	0.217MetTrp: 0.217 ± 0.012	0.274MetTyr: 0.274 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.027AsnAla: 3.027 ± 0.046	0.216AsnCys: 0.216 ± 0.012	1.319AsnAsp: 1.319 ± 0.032	1.193AsnGlu: 1.193 ± 0.027	0.885AsnPhe: 0.885 ± 0.026	2.29AsnGly: 2.29 ± 0.041	0.462AsnHis: 0.462 ± 0.017	1.202AsnIle: 1.202 ± 0.033	0.575AsnLys: 0.575 ± 0.022	2.3AsnLeu: 2.3 ± 0.04	0.566AsnMet: 0.566 ± 0.02	0.571AsnAsn: 0.571 ± 0.021	1.709AsnPro: 1.709 ± 0.041	0.685AsnGln: 0.685 ± 0.023	1.866AsnArg: 1.866 ± 0.041	1.023AsnSer: 1.023 ± 0.027	1.133AsnThr: 1.133 ± 0.028	1.73AsnVal: 1.73 ± 0.038	0.368AsnTrp: 0.368 ± 0.017	0.622AsnTyr: 0.622 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.845ProAla: 6.845 ± 0.086	0.333ProCys: 0.333 ± 0.015	3.912ProAsp: 3.912 ± 0.056	3.444ProGlu: 3.444 ± 0.056	2.005ProPhe: 2.005 ± 0.039	4.627ProGly: 4.627 ± 0.06	1.116ProHis: 1.116 ± 0.027	2.165ProIle: 2.165 ± 0.039	1.486ProLys: 1.486 ± 0.033	4.634ProLeu: 4.634 ± 0.059	1.093ProMet: 1.093 ± 0.029	1.159ProAsn: 1.159 ± 0.027	2.473ProPro: 2.473 ± 0.053	1.667ProGln: 1.667 ± 0.03	3.103ProArg: 3.103 ± 0.05	2.453ProSer: 2.453 ± 0.041	2.149ProThr: 2.149 ± 0.04	4.448ProVal: 4.448 ± 0.065	0.65ProTrp: 0.65 ± 0.023	1.164ProTyr: 1.164 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.919GlnAla: 3.919 ± 0.058	0.204GlnCys: 0.204 ± 0.014	1.469GlnAsp: 1.469 ± 0.034	1.388GlnGlu: 1.388 ± 0.029	0.956GlnPhe: 0.956 ± 0.024	2.15GlnGly: 2.15 ± 0.037	0.531GlnHis: 0.531 ± 0.021	1.607GlnIle: 1.607 ± 0.031	1.051GlnLys: 1.051 ± 0.026	2.395GlnLeu: 2.395 ± 0.046	0.804GlnMet: 0.804 ± 0.023	0.787GlnAsn: 0.787 ± 0.024	1.462GlnPro: 1.462 ± 0.035	0.968GlnGln: 0.968 ± 0.03	2.22GlnArg: 2.22 ± 0.042	1.501GlnSer: 1.501 ± 0.034	1.574GlnThr: 1.574 ± 0.035	1.949GlnVal: 1.949 ± 0.039	0.343GlnTrp: 0.343 ± 0.015	0.477GlnTyr: 0.477 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.444ArgAla: 9.444 ± 0.101	0.529ArgCys: 0.529 ± 0.021	4.592ArgAsp: 4.592 ± 0.06	4.271ArgGlu: 4.271 ± 0.066	3.211ArgPhe: 3.211 ± 0.047	5.124ArgGly: 5.124 ± 0.059	1.896ArgHis: 1.896 ± 0.041	4.076ArgIle: 4.076 ± 0.053	2.276ArgLys: 2.276 ± 0.046	8.517ArgLeu: 8.517 ± 0.097	1.949ArgMet: 1.949 ± 0.037	1.808ArgAsn: 1.808 ± 0.036	3.933ArgPro: 3.933 ± 0.062	2.563ArgGln: 2.563 ± 0.046	6.462ArgArg: 6.462 ± 0.098	3.512ArgSer: 3.512 ± 0.051	3.442ArgThr: 3.442 ± 0.048	4.923ArgVal: 4.923 ± 0.062	0.967ArgTrp: 0.967 ± 0.027	1.748ArgTyr: 1.748 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	5.927SerAla: 5.927 ± 0.064	0.397SerCys: 0.397 ± 0.019	2.693SerAsp: 2.693 ± 0.045	2.604SerGlu: 2.604 ± 0.043	2.188SerPhe: 2.188 ± 0.039	5.432SerGly: 5.432 ± 0.071	1.008SerHis: 1.008 ± 0.029	2.46SerIle: 2.46 ± 0.046	1.34SerLys: 1.34 ± 0.034	4.99SerLeu: 4.99 ± 0.06	1.237SerMet: 1.237 ± 0.027	1.137SerAsn: 1.137 ± 0.026	2.527SerPro: 2.527 ± 0.039	1.443SerGln: 1.443 ± 0.039	3.507SerArg: 3.507 ± 0.052	2.455SerSer: 2.455 ± 0.046	2.449SerThr: 2.449 ± 0.046	3.799SerVal: 3.799 ± 0.054	0.726SerTrp: 0.726 ± 0.02	1.179SerTyr: 1.179 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.347ThrAla: 6.347 ± 0.075	0.394ThrCys: 0.394 ± 0.019	2.663ThrAsp: 2.663 ± 0.039	2.479ThrGlu: 2.479 ± 0.043	1.891ThrPhe: 1.891 ± 0.036	5.285ThrGly: 5.285 ± 0.068	1.02ThrHis: 1.02 ± 0.028	2.847ThrIle: 2.847 ± 0.046	1.28ThrLys: 1.28 ± 0.03	5.464ThrLeu: 5.464 ± 0.059	1.307ThrMet: 1.307 ± 0.032	1.096ThrAsn: 1.096 ± 0.03	3.001ThrPro: 3.001 ± 0.046	1.237ThrGln: 1.237 ± 0.027	3.46ThrArg: 3.46 ± 0.05	2.353ThrSer: 2.353 ± 0.042	2.676ThrThr: 2.676 ± 0.045	4.678ThrVal: 4.678 ± 0.064	0.607ThrTrp: 0.607 ± 0.022	1.127ThrTyr: 1.127 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.079ValAla: 10.079 ± 0.096	0.653ValCys: 0.653 ± 0.024	4.5ValAsp: 4.5 ± 0.057	4.977ValGlu: 4.977 ± 0.064	3.094ValPhe: 3.094 ± 0.048	5.735ValGly: 5.735 ± 0.076	1.408ValHis: 1.408 ± 0.026	3.769ValIle: 3.769 ± 0.052	2.14ValLys: 2.14 ± 0.038	7.703ValLeu: 7.703 ± 0.087	1.901ValMet: 1.901 ± 0.037	1.854ValAsn: 1.854 ± 0.034	3.854ValPro: 3.854 ± 0.046	1.71ValGln: 1.71 ± 0.034	5.212ValArg: 5.212 ± 0.064	4.355ValSer: 4.355 ± 0.063	4.261ValThr: 4.261 ± 0.052	6.227ValVal: 6.227 ± 0.079	0.949ValTrp: 0.949 ± 0.027	1.523ValTyr: 1.523 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.23TrpAla: 1.23 ± 0.035	0.137TrpCys: 0.137 ± 0.009	0.575TrpAsp: 0.575 ± 0.02	0.541TrpGlu: 0.541 ± 0.019	0.546TrpPhe: 0.546 ± 0.018	0.874TrpGly: 0.874 ± 0.024	0.313TrpHis: 0.313 ± 0.015	0.622TrpIle: 0.622 ± 0.02	0.447TrpLys: 0.447 ± 0.02	1.649TrpLeu: 1.649 ± 0.037	0.363TrpMet: 0.363 ± 0.015	0.43TrpAsn: 0.43 ± 0.019	0.719TrpPro: 0.719 ± 0.021	0.539TrpGln: 0.539 ± 0.018	1.192TrpArg: 1.192 ± 0.03	0.839TrpSer: 0.839 ± 0.025	0.752TrpThr: 0.752 ± 0.023	0.759TrpVal: 0.759 ± 0.026	0.217TrpTrp: 0.217 ± 0.013	0.291TrpTyr: 0.291 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.534TyrAla: 2.534 ± 0.049	0.248TyrCys: 0.248 ± 0.012	1.435TyrAsp: 1.435 ± 0.029	1.211TyrGlu: 1.211 ± 0.027	0.948TyrPhe: 0.948 ± 0.026	2.153TyrGly: 2.153 ± 0.04	0.473TyrHis: 0.473 ± 0.018	0.828TyrIle: 0.828 ± 0.027	0.559TyrLys: 0.559 ± 0.023	2.295TyrLeu: 2.295 ± 0.039	0.414TyrMet: 0.414 ± 0.018	0.524TyrAsn: 0.524 ± 0.02	1.098TyrPro: 1.098 ± 0.028	0.685TyrGln: 0.685 ± 0.022	1.932TyrArg: 1.932 ± 0.042	1.072TyrSer: 1.072 ± 0.031	1.024TyrThr: 1.024 ± 0.029	1.679TyrVal: 1.679 ± 0.038	0.354TyrTrp: 0.354 ± 0.017	0.581TyrTyr: 0.581 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4621 proteins (1462021 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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