Staphylococcus virus CNPH82
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A1BTX4|A1BTX4_9CAUD Uncharacterized protein OS=Staphylococcus virus CNPH82 OX=398839 GN=cn23 PE=4 SV=1
MM1 pKa = 7.52 NGRR4 pKa = 11.84 SDD6 pKa = 3.59 EE7 pKa = 4.24 MKK9 pKa = 10.64 VVYY12 pKa = 9.59 LWKK15 pKa = 10.26 NGQAIIVHH23 pKa = 6.01 KK24 pKa = 10.76 NEE26 pKa = 3.61 EE27 pKa = 4.13 DD28 pKa = 3.72 EE29 pKa = 4.52 YY30 pKa = 11.45 VYY32 pKa = 10.65 PDD34 pKa = 3.75 EE35 pKa = 5.4 KK36 pKa = 9.76 WTEE39 pKa = 3.87 NQPPQGIILPCYY51 pKa = 9.8 YY52 pKa = 10.33 DD53 pKa = 3.45 GKK55 pKa = 10.21 QWVGQTQDD63 pKa = 3.78 EE64 pKa = 4.6 LEE66 pKa = 4.26 KK67 pKa = 10.23 MLPEE71 pKa = 4.0 VEE73 pKa = 4.68 IPVDD77 pKa = 4.01 DD78 pKa = 4.81 KK79 pKa = 11.74 DD80 pKa = 3.4 IAIAKK85 pKa = 7.34 LTSLVVDD92 pKa = 4.38 LQEE95 pKa = 4.31 EE96 pKa = 4.77 VMSLKK101 pKa = 10.75 QNIALITEE109 pKa = 4.14 EE110 pKa = 4.46 QANQKK115 pKa = 10.36 LGEE118 pKa = 4.04 AA119 pKa = 4.5
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.384
IPC2_protein 4.304
IPC_protein 4.215
Toseland 4.05
ProMoST 4.317
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.024
Rodwell 4.05
Grimsley 3.961
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.062
EMBOSS 4.05
Sillero 4.317
Patrickios 3.694
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.243
Protein with the highest isoelectric point:
>tr|A1BTW8|A1BTW8_9CAUD Putative phage tape measure protein OS=Staphylococcus virus CNPH82 OX=398839 GN=cn17 PE=4 SV=1
MM1 pKa = 7.54 SLTPNEE7 pKa = 3.89 WKK9 pKa = 10.31 DD10 pKa = 3.28 WVVGRR15 pKa = 11.84 RR16 pKa = 11.84 LALLDD21 pKa = 3.68 EE22 pKa = 4.38 QEE24 pKa = 4.25 NLLFGAQANGLVQAGKK40 pKa = 9.58 SLKK43 pKa = 10.18 RR44 pKa = 11.84 LQKK47 pKa = 8.59 QLEE50 pKa = 3.97 RR51 pKa = 11.84 ARR53 pKa = 11.84 YY54 pKa = 6.86 EE55 pKa = 3.64 VRR57 pKa = 11.84 GQSEE61 pKa = 4.38 EE62 pKa = 4.06 YY63 pKa = 10.73 EE64 pKa = 4.03 RR65 pKa = 11.84 MKK67 pKa = 10.59 EE68 pKa = 4.04 RR69 pKa = 11.84 KK70 pKa = 8.73 LAHH73 pKa = 6.28 NKK75 pKa = 10.13 RR76 pKa = 11.84 IRR78 pKa = 11.84 NVQKK82 pKa = 9.93 QGTRR86 pKa = 11.84 RR87 pKa = 11.84 FMNSLRR93 pKa = 11.84 NTSQKK98 pKa = 11.01 GGG100 pKa = 3.49
Molecular weight: 11.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.736
IPC_protein 10.555
Toseland 10.979
ProMoST 10.716
Dawson 11.038
Bjellqvist 10.745
Wikipedia 11.257
Rodwell 11.272
Grimsley 11.067
Solomon 11.213
Lehninger 11.169
Nozaki 10.95
DTASelect 10.745
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.965
Patrickios 11.008
IPC_peptide 11.213
IPC2_peptide 9.458
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
13554
34
1034
208.5
23.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.283 ± 0.463
0.664 ± 0.121
6.397 ± 0.332
7.326 ± 0.548
3.792 ± 0.197
6.168 ± 0.378
1.749 ± 0.159
7.164 ± 0.26
9.333 ± 0.421
7.607 ± 0.257
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.166
6.965 ± 0.347
2.804 ± 0.195
4.087 ± 0.253
4.523 ± 0.229
5.814 ± 0.3
5.851 ± 0.273
6.197 ± 0.224
1.343 ± 0.141
4.464 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here