Branchiibius sp. NY16-3462-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermacoccaceae; Branchiibius; unclassified Branchiibius

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3548 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A151BXN6|A0A151BXN6_9MICO ABC transporter OS=Branchiibius sp. NY16-3462-2 OX=1807500 GN=AZH51_08450 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.52PGIYY6 pKa = 10.32LLQDD10 pKa = 3.21GSEE13 pKa = 4.46EE14 pKa = 4.56YY15 pKa = 9.44VTSLTFEE22 pKa = 4.1QEE24 pKa = 3.71PDD26 pKa = 3.36LGEE29 pKa = 4.49GDD31 pKa = 3.71SPARR35 pKa = 11.84ISQYY39 pKa = 10.54PLEE42 pKa = 4.86DD43 pKa = 3.46LLDD46 pKa = 4.02RR47 pKa = 11.84FNVWVSDD54 pKa = 3.94FYY56 pKa = 11.25PDD58 pKa = 4.39LNSSSARR65 pKa = 11.84TCYY68 pKa = 10.34QEE70 pKa = 4.86FAAADD75 pKa = 3.78LADD78 pKa = 3.21IQAFRR83 pKa = 11.84EE84 pKa = 4.06IIGRR88 pKa = 11.84HH89 pKa = 4.1VYY91 pKa = 10.06LRR93 pKa = 11.84NDD95 pKa = 3.33GAAVHH100 pKa = 6.19LVIDD104 pKa = 4.21

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A151BYR1|A0A151BYR1_9MICO Dipeptidase OS=Branchiibius sp. NY16-3462-2 OX=1807500 GN=AZH51_14640 PE=4 SV=1
MM1 pKa = 7.47TLLSQEE7 pKa = 4.15IRR9 pKa = 11.84PEE11 pKa = 4.2SIEE14 pKa = 4.02KK15 pKa = 10.04VRR17 pKa = 11.84STRR20 pKa = 11.84KK21 pKa = 8.95RR22 pKa = 11.84VTRR25 pKa = 11.84RR26 pKa = 11.84TSIVLTVVILIWFGLFAARR45 pKa = 11.84VLLGDD50 pKa = 3.76FTITIPDD57 pKa = 4.08FFRR60 pKa = 11.84IAFGGAQMPPATFILMEE77 pKa = 4.67SKK79 pKa = 10.8LPAAVAGTLAGLAFGASGSAFQTMLRR105 pKa = 11.84NPLASPDD112 pKa = 3.76VLGVTLGSSAAAVFALVVLGWSGLSMSLAALLGGMFVAAAINLLAGRR159 pKa = 11.84DD160 pKa = 3.46SAATYY165 pKa = 11.09RR166 pKa = 11.84MILIGIGISAGLSSFIQWMLQRR188 pKa = 11.84TSIYY192 pKa = 9.69RR193 pKa = 11.84AQDD196 pKa = 2.87AMVWLTGSLSVIPWDD211 pKa = 3.46GVLRR215 pKa = 11.84LAITVAVLLPLLVASLNGLRR235 pKa = 11.84LLEE238 pKa = 5.1LGDD241 pKa = 3.96DD242 pKa = 3.72TALALGLNAARR253 pKa = 11.84LRR255 pKa = 11.84LTVSVLVVILCAMATSVTGPIAFIGLLSGPIARR288 pKa = 11.84RR289 pKa = 11.84LCGGRR294 pKa = 11.84PNILVAAFVGAATVVGADD312 pKa = 3.51YY313 pKa = 10.72IGTYY317 pKa = 10.44LIPNTNLPVGVITGIAGAPVLIFLLLKK344 pKa = 10.59SRR346 pKa = 11.84ATIKK350 pKa = 10.91DD351 pKa = 3.35GG352 pKa = 3.92

Molecular weight: 36.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3548

0

3548

1140667

29

2494

321.5

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.878 ± 0.047

0.707 ± 0.012

6.347 ± 0.039

5.054 ± 0.041

2.956 ± 0.027

8.837 ± 0.036

2.125 ± 0.021

4.268 ± 0.026

2.313 ± 0.032

10.079 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.89 ± 0.017

2.079 ± 0.023

5.519 ± 0.028

3.394 ± 0.021

6.875 ± 0.046

5.723 ± 0.033

6.346 ± 0.045

8.896 ± 0.038

1.624 ± 0.021

2.089 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski