Amino acid dipepetide frequency for Erythrobacter sp. YT30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.798AlaAla: 14.798 ± 0.162
1.032AlaCys: 1.032 ± 0.038
7.046AlaAsp: 7.046 ± 0.09
8.046AlaGlu: 8.046 ± 0.107
4.458AlaPhe: 4.458 ± 0.081
9.693AlaGly: 9.693 ± 0.111
2.095AlaHis: 2.095 ± 0.052
6.801AlaIle: 6.801 ± 0.098
4.825AlaLys: 4.825 ± 0.087
12.287AlaLeu: 12.287 ± 0.133
3.725AlaMet: 3.725 ± 0.065
3.57AlaAsn: 3.57 ± 0.063
5.318AlaPro: 5.318 ± 0.095
4.355AlaGln: 4.355 ± 0.077
7.81AlaArg: 7.81 ± 0.112
6.874AlaSer: 6.874 ± 0.098
5.536AlaThr: 5.536 ± 0.083
7.387AlaVal: 7.387 ± 0.094
1.282AlaTrp: 1.282 ± 0.041
2.324AlaTyr: 2.324 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.003CysAla: 1.003 ± 0.033
0.075CysCys: 0.075 ± 0.009
0.633CysAsp: 0.633 ± 0.029
0.576CysGlu: 0.576 ± 0.026
0.303CysPhe: 0.303 ± 0.019
0.888CysGly: 0.888 ± 0.034
0.18CysHis: 0.18 ± 0.012
0.402CysIle: 0.402 ± 0.021
0.211CysLys: 0.211 ± 0.014
0.693CysLeu: 0.693 ± 0.028
0.137CysMet: 0.137 ± 0.012
0.258CysAsn: 0.258 ± 0.018
0.414CysPro: 0.414 ± 0.022
0.197CysGln: 0.197 ± 0.014
0.47CysArg: 0.47 ± 0.02
0.5CysSer: 0.5 ± 0.025
0.382CysThr: 0.382 ± 0.023
0.591CysVal: 0.591 ± 0.027
0.106CysTrp: 0.106 ± 0.012
0.159CysTyr: 0.159 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.432AspAla: 7.432 ± 0.077
0.557AspCys: 0.557 ± 0.027
3.872AspAsp: 3.872 ± 0.081
4.503AspGlu: 4.503 ± 0.086
2.538AspPhe: 2.538 ± 0.047
5.652AspGly: 5.652 ± 0.085
1.263AspHis: 1.263 ± 0.037
3.301AspIle: 3.301 ± 0.053
2.083AspLys: 2.083 ± 0.044
5.984AspLeu: 5.984 ± 0.083
1.512AspMet: 1.512 ± 0.043
1.841AspAsn: 1.841 ± 0.044
3.872AspPro: 3.872 ± 0.078
2.047AspGln: 2.047 ± 0.049
4.09AspArg: 4.09 ± 0.074
2.356AspSer: 2.356 ± 0.051
3.062AspThr: 3.062 ± 0.049
4.202AspVal: 4.202 ± 0.072
1.154AspTrp: 1.154 ± 0.034
1.645AspTyr: 1.645 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
8.496GluAla: 8.496 ± 0.109
0.472GluCys: 0.472 ± 0.022
3.966GluAsp: 3.966 ± 0.076
4.892GluGlu: 4.892 ± 0.089
2.271GluPhe: 2.271 ± 0.05
5.277GluGly: 5.277 ± 0.075
1.259GluHis: 1.259 ± 0.035
3.755GluIle: 3.755 ± 0.069
2.81GluLys: 2.81 ± 0.074
6.202GluLeu: 6.202 ± 0.083
1.854GluMet: 1.854 ± 0.044
2.063GluAsn: 2.063 ± 0.047
3.033GluPro: 3.033 ± 0.055
2.529GluGln: 2.529 ± 0.055
5.051GluArg: 5.051 ± 0.083
2.808GluSer: 2.808 ± 0.055
3.987GluThr: 3.987 ± 0.066
4.267GluVal: 4.267 ± 0.066
1.011GluTrp: 1.011 ± 0.042
1.302GluTyr: 1.302 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.969PheAla: 4.969 ± 0.082
0.37PheCys: 0.37 ± 0.016
3.009PheAsp: 3.009 ± 0.056
2.791PheGlu: 2.791 ± 0.055
1.513PhePhe: 1.513 ± 0.049
3.899PheGly: 3.899 ± 0.066
0.636PheHis: 0.636 ± 0.029
1.746PheIle: 1.746 ± 0.044
1.114PheLys: 1.114 ± 0.034
3.125PheLeu: 3.125 ± 0.066
0.841PheMet: 0.841 ± 0.034
1.209PheAsn: 1.209 ± 0.039
1.613PhePro: 1.613 ± 0.04
1.003PheGln: 1.003 ± 0.029
2.034PheArg: 2.034 ± 0.044
2.375PheSer: 2.375 ± 0.051
2.205PheThr: 2.205 ± 0.061
2.797PheVal: 2.797 ± 0.055
0.566PheTrp: 0.566 ± 0.031
0.981PheTyr: 0.981 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
9.029GlyAla: 9.029 ± 0.112
0.79GlyCys: 0.79 ± 0.031
5.236GlyAsp: 5.236 ± 0.087
6.042GlyGlu: 6.042 ± 0.086
3.812GlyPhe: 3.812 ± 0.063
7.418GlyGly: 7.418 ± 0.119
1.624GlyHis: 1.624 ± 0.046
4.552GlyIle: 4.552 ± 0.076
3.503GlyLys: 3.503 ± 0.068
8.083GlyLeu: 8.083 ± 0.105
2.291GlyMet: 2.291 ± 0.052
2.538GlyAsn: 2.538 ± 0.071
3.256GlyPro: 3.256 ± 0.057
2.763GlyGln: 2.763 ± 0.051
5.18GlyArg: 5.18 ± 0.077
5.221GlySer: 5.221 ± 0.073
4.683GlyThr: 4.683 ± 0.072
5.771GlyVal: 5.771 ± 0.079
1.377GlyTrp: 1.377 ± 0.032
2.147GlyTyr: 2.147 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
2.071HisAla: 2.071 ± 0.049
0.237HisCys: 0.237 ± 0.017
1.152HisAsp: 1.152 ± 0.039
1.064HisGlu: 1.064 ± 0.041
0.866HisPhe: 0.866 ± 0.031
1.723HisGly: 1.723 ± 0.045
0.502HisHis: 0.502 ± 0.024
0.926HisIle: 0.926 ± 0.033
0.54HisLys: 0.54 ± 0.022
1.687HisLeu: 1.687 ± 0.043
0.422HisMet: 0.422 ± 0.024
0.471HisAsn: 0.471 ± 0.02
1.156HisPro: 1.156 ± 0.039
0.554HisGln: 0.554 ± 0.024
1.17HisArg: 1.17 ± 0.036
1.085HisSer: 1.085 ± 0.031
0.804HisThr: 0.804 ± 0.029
1.232HisVal: 1.232 ± 0.038
0.306HisTrp: 0.306 ± 0.019
0.557HisTyr: 0.557 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
8.038IleAla: 8.038 ± 0.097
0.492IleCys: 0.492 ± 0.024
3.94IleAsp: 3.94 ± 0.061
4.34IleGlu: 4.34 ± 0.077
1.7IlePhe: 1.7 ± 0.042
5.157IleGly: 5.157 ± 0.081
0.847IleHis: 0.847 ± 0.03
2.629IleIle: 2.629 ± 0.055
1.677IleLys: 1.677 ± 0.049
4.117IleLeu: 4.117 ± 0.076
1.023IleMet: 1.023 ± 0.033
1.606IleAsn: 1.606 ± 0.046
2.526IlePro: 2.526 ± 0.054
1.288IleGln: 1.288 ± 0.033
2.938IleArg: 2.938 ± 0.05
3.072IleSer: 3.072 ± 0.055
3.112IleThr: 3.112 ± 0.065
3.96IleVal: 3.96 ± 0.072
0.72IleTrp: 0.72 ± 0.031
1.132IleTyr: 1.132 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
4.52LysAla: 4.52 ± 0.072
0.213LysCys: 0.213 ± 0.015
1.859LysAsp: 1.859 ± 0.05
1.958LysGlu: 1.958 ± 0.052
1.015LysPhe: 1.015 ± 0.03
2.901LysGly: 2.901 ± 0.063
0.727LysHis: 0.727 ± 0.031
1.65LysIle: 1.65 ± 0.049
1.528LysLys: 1.528 ± 0.061
3.591LysLeu: 3.591 ± 0.067
0.917LysMet: 0.917 ± 0.032
0.882LysAsn: 0.882 ± 0.028
2.1LysPro: 2.1 ± 0.047
1.161LysGln: 1.161 ± 0.037
2.772LysArg: 2.772 ± 0.065
2.076LysSer: 2.076 ± 0.051
1.971LysThr: 1.971 ± 0.059
2.439LysVal: 2.439 ± 0.061
0.499LysTrp: 0.499 ± 0.022
0.697LysTyr: 0.697 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
12.346LeuAla: 12.346 ± 0.128
0.757LeuCys: 0.757 ± 0.026
6.034LeuAsp: 6.034 ± 0.084
6.026LeuGlu: 6.026 ± 0.089
3.568LeuPhe: 3.568 ± 0.076
8.0LeuGly: 8.0 ± 0.09
1.593LeuHis: 1.593 ± 0.041
5.122LeuIle: 5.122 ± 0.087
3.23LeuLys: 3.23 ± 0.063
8.414LeuLeu: 8.414 ± 0.138
2.142LeuMet: 2.142 ± 0.054
2.62LeuAsn: 2.62 ± 0.056
4.973LeuPro: 4.973 ± 0.071
2.523LeuGln: 2.523 ± 0.057
5.816LeuArg: 5.816 ± 0.099
6.273LeuSer: 6.273 ± 0.09
5.425LeuThr: 5.425 ± 0.069
6.666LeuVal: 6.666 ± 0.101
1.088LeuTrp: 1.088 ± 0.039
1.945LeuTyr: 1.945 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
3.076MetAla: 3.076 ± 0.06
0.173MetCys: 0.173 ± 0.014
1.238MetAsp: 1.238 ± 0.038
1.342MetGlu: 1.342 ± 0.037
0.762MetPhe: 0.762 ± 0.03
2.023MetGly: 2.023 ± 0.055
0.47MetHis: 0.47 ± 0.022
1.482MetIle: 1.482 ± 0.04
1.04MetLys: 1.04 ± 0.037
2.596MetLeu: 2.596 ± 0.057
0.699MetMet: 0.699 ± 0.033
0.776MetAsn: 0.776 ± 0.029
1.431MetPro: 1.431 ± 0.041
0.832MetGln: 0.832 ± 0.027
1.8MetArg: 1.8 ± 0.045
1.629MetSer: 1.629 ± 0.045
1.566MetThr: 1.566 ± 0.035
1.617MetVal: 1.617 ± 0.042
0.238MetTrp: 0.238 ± 0.017
0.311MetTyr: 0.311 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.581AsnAla: 3.581 ± 0.068
0.273AsnCys: 0.273 ± 0.019
1.783AsnAsp: 1.783 ± 0.044
1.672AsnGlu: 1.672 ± 0.041
1.186AsnPhe: 1.186 ± 0.043
2.565AsnGly: 2.565 ± 0.058
0.512AsnHis: 0.512 ± 0.025
1.553AsnIle: 1.553 ± 0.045
0.815AsnLys: 0.815 ± 0.031
2.695AsnLeu: 2.695 ± 0.048
0.65AsnMet: 0.65 ± 0.028
0.811AsnAsn: 0.811 ± 0.039
2.021AsnPro: 2.021 ± 0.046
0.945AsnGln: 0.945 ± 0.033
1.988AsnArg: 1.988 ± 0.043
1.635AsnSer: 1.635 ± 0.045
1.505AsnThr: 1.505 ± 0.046
2.052AsnVal: 2.052 ± 0.053
0.499AsnTrp: 0.499 ± 0.023
0.721AsnTyr: 0.721 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
5.397ProAla: 5.397 ± 0.091
0.285ProCys: 0.285 ± 0.018
3.885ProAsp: 3.885 ± 0.066
4.092ProGlu: 4.092 ± 0.081
1.964ProPhe: 1.964 ± 0.053
3.997ProGly: 3.997 ± 0.067
0.93ProHis: 0.93 ± 0.029
2.659ProIle: 2.659 ± 0.049
1.76ProLys: 1.76 ± 0.042
4.437ProLeu: 4.437 ± 0.082
1.135ProMet: 1.135 ± 0.037
1.476ProAsn: 1.476 ± 0.045
2.304ProPro: 2.304 ± 0.074
1.713ProGln: 1.713 ± 0.044
2.569ProArg: 2.569 ± 0.057
3.007ProSer: 3.007 ± 0.058
2.447ProThr: 2.447 ± 0.051
3.729ProVal: 3.729 ± 0.059
0.594ProTrp: 0.594 ± 0.027
1.009ProTyr: 1.009 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.629GlnAla: 3.629 ± 0.07
0.257GlnCys: 0.257 ± 0.016
1.609GlnAsp: 1.609 ± 0.045
1.64GlnGlu: 1.64 ± 0.038
1.333GlnPhe: 1.333 ± 0.037
2.363GlnGly: 2.363 ± 0.048
0.627GlnHis: 0.627 ± 0.027
2.043GlnIle: 2.043 ± 0.046
1.109GlnLys: 1.109 ± 0.033
3.238GlnLeu: 3.238 ± 0.061
0.967GlnMet: 0.967 ± 0.033
0.968GlnAsn: 0.968 ± 0.036
1.586GlnPro: 1.586 ± 0.034
1.158GlnGln: 1.158 ± 0.039
2.227GlnArg: 2.227 ± 0.053
2.255GlnSer: 2.255 ± 0.051
1.786GlnThr: 1.786 ± 0.043
2.263GlnVal: 2.263 ± 0.049
0.476GlnTrp: 0.476 ± 0.025
0.721GlnTyr: 0.721 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
6.987ArgAla: 6.987 ± 0.099
0.441ArgCys: 0.441 ± 0.022
4.108ArgAsp: 4.108 ± 0.077
4.619ArgGlu: 4.619 ± 0.083
2.897ArgPhe: 2.897 ± 0.06
4.576ArgGly: 4.576 ± 0.08
1.242ArgHis: 1.242 ± 0.038
3.927ArgIle: 3.927 ± 0.069
2.358ArgLys: 2.358 ± 0.053
6.535ArgLeu: 6.535 ± 0.107
1.703ArgMet: 1.703 ± 0.042
1.92ArgAsn: 1.92 ± 0.044
2.752ArgPro: 2.752 ± 0.051
2.143ArgGln: 2.143 ± 0.055
4.219ArgArg: 4.219 ± 0.075
3.629ArgSer: 3.629 ± 0.06
3.067ArgThr: 3.067 ± 0.061
4.326ArgVal: 4.326 ± 0.083
0.97ArgTrp: 0.97 ± 0.031
1.664ArgTyr: 1.664 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.58SerAla: 6.58 ± 0.082
0.404SerCys: 0.404 ± 0.02
3.867SerAsp: 3.867 ± 0.062
3.906SerGlu: 3.906 ± 0.066
2.314SerPhe: 2.314 ± 0.047
5.688SerGly: 5.688 ± 0.078
1.047SerHis: 1.047 ± 0.032
3.012SerIle: 3.012 ± 0.059
1.902SerLys: 1.902 ± 0.045
5.452SerLeu: 5.452 ± 0.085
1.351SerMet: 1.351 ± 0.041
1.803SerAsn: 1.803 ± 0.046
2.775SerPro: 2.775 ± 0.064
1.953SerGln: 1.953 ± 0.046
3.519SerArg: 3.519 ± 0.066
3.526SerSer: 3.526 ± 0.061
2.745SerThr: 2.745 ± 0.057
3.901SerVal: 3.901 ± 0.066
0.765SerTrp: 0.765 ± 0.032
1.377SerTyr: 1.377 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
5.933ThrAla: 5.933 ± 0.075
0.421ThrCys: 0.421 ± 0.022
3.1ThrAsp: 3.1 ± 0.06
2.892ThrGlu: 2.892 ± 0.056
2.088ThrPhe: 2.088 ± 0.058
5.228ThrGly: 5.228 ± 0.072
0.97ThrHis: 0.97 ± 0.032
3.087ThrIle: 3.087 ± 0.063
1.698ThrLys: 1.698 ± 0.047
5.334ThrLeu: 5.334 ± 0.078
1.212ThrMet: 1.212 ± 0.036
1.529ThrAsn: 1.529 ± 0.048
3.074ThrPro: 3.074 ± 0.064
1.747ThrGln: 1.747 ± 0.043
3.306ThrArg: 3.306 ± 0.064
3.03ThrSer: 3.03 ± 0.06
2.729ThrThr: 2.729 ± 0.06
3.809ThrVal: 3.809 ± 0.08
0.618ThrTrp: 0.618 ± 0.026
1.294ThrTyr: 1.294 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
7.755ValAla: 7.755 ± 0.109
0.575ValCys: 0.575 ± 0.022
4.22ValAsp: 4.22 ± 0.074
4.773ValGlu: 4.773 ± 0.069
2.67ValPhe: 2.67 ± 0.065
5.216ValGly: 5.216 ± 0.083
1.19ValHis: 1.19 ± 0.038
3.971ValIle: 3.971 ± 0.071
2.133ValLys: 2.133 ± 0.059
6.386ValLeu: 6.386 ± 0.091
1.724ValMet: 1.724 ± 0.044
2.069ValAsn: 2.069 ± 0.046
3.559ValPro: 3.559 ± 0.061
2.023ValGln: 2.023 ± 0.048
4.379ValArg: 4.379 ± 0.068
4.247ValSer: 4.247 ± 0.086
4.253ValThr: 4.253 ± 0.081
4.786ValVal: 4.786 ± 0.083
0.836ValTrp: 0.836 ± 0.033
1.356ValTyr: 1.356 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.227TrpAla: 1.227 ± 0.032
0.112TrpCys: 0.112 ± 0.01
0.785TrpAsp: 0.785 ± 0.029
0.765TrpGlu: 0.765 ± 0.034
0.572TrpPhe: 0.572 ± 0.025
0.967TrpGly: 0.967 ± 0.03
0.333TrpHis: 0.333 ± 0.019
0.724TrpIle: 0.724 ± 0.032
0.48TrpLys: 0.48 ± 0.024
1.612TrpLeu: 1.612 ± 0.05
0.37TrpMet: 0.37 ± 0.019
0.412TrpAsn: 0.412 ± 0.019
0.639TrpPro: 0.639 ± 0.028
0.582TrpGln: 0.582 ± 0.023
1.137TrpArg: 1.137 ± 0.036
0.907TrpSer: 0.907 ± 0.03
0.703TrpThr: 0.703 ± 0.026
0.827TrpVal: 0.827 ± 0.035
0.25TrpTrp: 0.25 ± 0.015
0.286TrpTyr: 0.286 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.397TyrAla: 2.397 ± 0.05
0.245TyrCys: 0.245 ± 0.017
1.622TyrAsp: 1.622 ± 0.046
1.366TyrGlu: 1.366 ± 0.04
0.964TyrPhe: 0.964 ± 0.032
2.085TyrGly: 2.085 ± 0.057
0.475TyrHis: 0.475 ± 0.023
0.973TyrIle: 0.973 ± 0.034
0.661TyrLys: 0.661 ± 0.027
2.092TyrLeu: 2.092 ± 0.058
0.426TyrMet: 0.426 ± 0.022
0.64TyrAsn: 0.64 ± 0.025
0.986TyrPro: 0.986 ± 0.034
0.741TyrGln: 0.741 ± 0.029
1.607TyrArg: 1.607 ± 0.043
1.383TyrSer: 1.383 ± 0.042
1.13TyrThr: 1.13 ± 0.037
1.499TyrVal: 1.499 ± 0.041
0.335TyrTrp: 0.335 ± 0.022
0.594TyrTyr: 0.594 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2997 proteins (959551 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski