Arthrobacter phage Andrew
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2KDA3|A0A3G2KDA3_9CAUD Uncharacterized protein OS=Arthrobacter phage Andrew OX=2419946 GN=61 PE=4 SV=1
MM1 pKa = 7.65 SEE3 pKa = 3.83 WTVKK7 pKa = 10.77 GDD9 pKa = 3.69 GFAEE13 pKa = 5.01 GPTIEE18 pKa = 4.59 VEE20 pKa = 4.16 NGSTPILVSAVWTNEE35 pKa = 3.52 DD36 pKa = 4.01 GVCVSVDD43 pKa = 3.39 SGDD46 pKa = 3.68 EE47 pKa = 3.85 PLPARR52 pKa = 11.84 VAWRR56 pKa = 11.84 VCAAIMDD63 pKa = 4.99 LAAMPSPEE71 pKa = 4.46 LSSSS75 pKa = 3.26
Molecular weight: 7.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.257
IPC2_protein 3.846
IPC_protein 3.694
Toseland 3.528
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.452
Solomon 3.643
Lehninger 3.605
Nozaki 3.821
DTASelect 3.948
Thurlkill 3.592
EMBOSS 3.63
Sillero 3.821
Patrickios 3.465
IPC_peptide 3.643
IPC2_peptide 3.795
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A3G2KCV5|A0A3G2KCV5_9CAUD Uncharacterized protein OS=Arthrobacter phage Andrew OX=2419946 GN=22 PE=4 SV=1
MM1 pKa = 7.59 TGPSPAVRR9 pKa = 11.84 RR10 pKa = 11.84 QVIARR15 pKa = 11.84 DD16 pKa = 3.49 LSRR19 pKa = 11.84 CQWCGRR25 pKa = 11.84 HH26 pKa = 5.33 VRR28 pKa = 11.84 TDD30 pKa = 3.4 SGWYY34 pKa = 9.12 SLQHH38 pKa = 5.14 RR39 pKa = 11.84 RR40 pKa = 11.84 ARR42 pKa = 11.84 GMGGSKK48 pKa = 10.5 DD49 pKa = 3.35 KK50 pKa = 10.58 ATNQPANLILVHH62 pKa = 5.72 GTGTTEE68 pKa = 3.59 CHH70 pKa = 5.76 GWIEE74 pKa = 4.29 SQPAQAIGRR83 pKa = 11.84 GFRR86 pKa = 11.84 IAAAADD92 pKa = 3.88 PAKK95 pKa = 10.43 VPYY98 pKa = 9.4 MDD100 pKa = 2.94 WTGRR104 pKa = 11.84 EE105 pKa = 3.81 WLLTNDD111 pKa = 3.74 GRR113 pKa = 11.84 KK114 pKa = 9.4 LPPP117 pKa = 4.14
Molecular weight: 12.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.399
IPC_protein 10.043
Toseland 10.423
ProMoST 10.116
Dawson 10.54
Bjellqvist 10.277
Wikipedia 10.745
Rodwell 10.716
Grimsley 10.599
Solomon 10.657
Lehninger 10.628
Nozaki 10.467
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.482
Patrickios 10.496
IPC_peptide 10.657
IPC2_peptide 9.531
IPC2.peptide.svr19 8.461
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
12234
33
787
172.3
18.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.209 ± 0.443
0.727 ± 0.102
5.583 ± 0.167
6.204 ± 0.383
2.722 ± 0.179
9.833 ± 0.495
1.324 ± 0.124
3.58 ± 0.199
3.588 ± 0.246
9.179 ± 0.302
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.231 ± 0.145
2.33 ± 0.156
5.697 ± 0.3
2.967 ± 0.222
7.209 ± 0.423
5.468 ± 0.254
5.714 ± 0.298
8.378 ± 0.345
2.158 ± 0.131
1.896 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here