Amino acid dipepetide frequency for Arthrobacter phage Andrew

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.461AlaAla: 23.461 ± 1.669
1.144AlaCys: 1.144 ± 0.394
7.766AlaAsp: 7.766 ± 0.894
8.747AlaGlu: 8.747 ± 0.982
2.861AlaPhe: 2.861 ± 0.569
15.123AlaGly: 15.123 ± 1.552
1.88AlaHis: 1.88 ± 0.529
5.395AlaIle: 5.395 ± 0.562
3.433AlaLys: 3.433 ± 0.577
10.464AlaLeu: 10.464 ± 0.862
2.861AlaMet: 2.861 ± 0.446
2.779AlaAsn: 2.779 ± 0.424
6.54AlaPro: 6.54 ± 0.858
2.452AlaGln: 2.452 ± 0.418
9.156AlaArg: 9.156 ± 0.881
5.722AlaSer: 5.722 ± 0.55
5.64AlaThr: 5.64 ± 0.681
10.3AlaVal: 10.3 ± 1.008
3.515AlaTrp: 3.515 ± 0.596
2.779AlaTyr: 2.779 ± 0.488
0.0AlaXaa: 0.0 ± 0.0
Cys
0.736CysAla: 0.736 ± 0.268
0.0CysCys: 0.0 ± 0.0
0.163CysAsp: 0.163 ± 0.131
0.409CysGlu: 0.409 ± 0.163
0.082CysPhe: 0.082 ± 0.094
1.144CysGly: 1.144 ± 0.353
0.163CysHis: 0.163 ± 0.109
0.49CysIle: 0.49 ± 0.197
0.245CysLys: 0.245 ± 0.132
0.409CysLeu: 0.409 ± 0.198
0.082CysMet: 0.082 ± 0.075
0.163CysAsn: 0.163 ± 0.111
0.082CysPro: 0.082 ± 0.089
0.327CysGln: 0.327 ± 0.172
0.817CysArg: 0.817 ± 0.283
0.736CysSer: 0.736 ± 0.248
0.327CysThr: 0.327 ± 0.154
0.654CysVal: 0.654 ± 0.26
0.245CysTrp: 0.245 ± 0.146
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.64AspAla: 5.64 ± 0.639
0.245AspCys: 0.245 ± 0.133
3.025AspAsp: 3.025 ± 0.607
3.679AspGlu: 3.679 ± 0.585
1.88AspPhe: 1.88 ± 0.351
6.785AspGly: 6.785 ± 0.825
1.063AspHis: 1.063 ± 0.331
0.981AspIle: 0.981 ± 0.253
2.534AspLys: 2.534 ± 0.558
6.294AspLeu: 6.294 ± 0.793
1.144AspMet: 1.144 ± 0.403
1.144AspAsn: 1.144 ± 0.288
3.597AspPro: 3.597 ± 0.444
1.635AspGln: 1.635 ± 0.275
2.698AspArg: 2.698 ± 0.388
3.27AspSer: 3.27 ± 0.576
2.125AspThr: 2.125 ± 0.444
5.232AspVal: 5.232 ± 0.696
1.471AspTrp: 1.471 ± 0.341
1.39AspTyr: 1.39 ± 0.337
0.0AspXaa: 0.0 ± 0.0
Glu
7.848GluAla: 7.848 ± 0.905
0.736GluCys: 0.736 ± 0.206
2.779GluAsp: 2.779 ± 0.488
4.251GluGlu: 4.251 ± 0.672
1.88GluPhe: 1.88 ± 0.391
4.578GluGly: 4.578 ± 0.566
0.981GluHis: 0.981 ± 0.326
3.025GluIle: 3.025 ± 0.586
1.717GluLys: 1.717 ± 0.437
6.54GluLeu: 6.54 ± 0.762
1.471GluMet: 1.471 ± 0.412
1.226GluAsn: 1.226 ± 0.324
3.025GluPro: 3.025 ± 0.648
2.371GluGln: 2.371 ± 0.465
5.395GluArg: 5.395 ± 0.759
2.452GluSer: 2.452 ± 0.433
2.779GluThr: 2.779 ± 0.446
5.804GluVal: 5.804 ± 0.583
1.717GluTrp: 1.717 ± 0.387
1.471GluTyr: 1.471 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
2.861PheAla: 2.861 ± 0.414
0.163PheCys: 0.163 ± 0.113
2.125PheAsp: 2.125 ± 0.387
2.371PheGlu: 2.371 ± 0.466
0.817PhePhe: 0.817 ± 0.244
2.125PheGly: 2.125 ± 0.391
0.409PheHis: 0.409 ± 0.184
1.063PheIle: 1.063 ± 0.27
1.635PheLys: 1.635 ± 0.341
2.289PheLeu: 2.289 ± 0.421
0.736PheMet: 0.736 ± 0.2
0.981PheAsn: 0.981 ± 0.315
0.981PhePro: 0.981 ± 0.282
0.327PheGln: 0.327 ± 0.148
1.88PheArg: 1.88 ± 0.474
1.471PheSer: 1.471 ± 0.363
1.962PheThr: 1.962 ± 0.452
1.962PheVal: 1.962 ± 0.415
0.327PheTrp: 0.327 ± 0.152
0.736PheTyr: 0.736 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
12.262GlyAla: 12.262 ± 1.207
0.736GlyCys: 0.736 ± 0.221
5.068GlyAsp: 5.068 ± 0.737
4.987GlyGlu: 4.987 ± 0.693
2.779GlyPhe: 2.779 ± 0.542
10.3GlyGly: 10.3 ± 1.119
1.226GlyHis: 1.226 ± 0.312
3.679GlyIle: 3.679 ± 0.809
2.616GlyLys: 2.616 ± 0.447
9.319GlyLeu: 9.319 ± 1.054
2.861GlyMet: 2.861 ± 0.444
1.553GlyAsn: 1.553 ± 0.356
5.886GlyPro: 5.886 ± 0.811
3.924GlyGln: 3.924 ± 0.587
8.338GlyArg: 8.338 ± 0.842
5.477GlySer: 5.477 ± 0.787
5.477GlyThr: 5.477 ± 0.669
7.194GlyVal: 7.194 ± 0.913
2.452GlyTrp: 2.452 ± 0.403
2.207GlyTyr: 2.207 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
2.125HisAla: 2.125 ± 0.357
0.082HisCys: 0.082 ± 0.089
0.736HisAsp: 0.736 ± 0.26
0.736HisGlu: 0.736 ± 0.255
0.49HisPhe: 0.49 ± 0.169
1.226HisGly: 1.226 ± 0.324
0.327HisHis: 0.327 ± 0.155
0.49HisIle: 0.49 ± 0.249
0.572HisLys: 0.572 ± 0.184
0.981HisLeu: 0.981 ± 0.336
0.49HisMet: 0.49 ± 0.19
0.49HisAsn: 0.49 ± 0.189
0.899HisPro: 0.899 ± 0.263
0.409HisGln: 0.409 ± 0.168
0.736HisArg: 0.736 ± 0.24
0.327HisSer: 0.327 ± 0.175
0.409HisThr: 0.409 ± 0.187
1.226HisVal: 1.226 ± 0.328
0.163HisTrp: 0.163 ± 0.105
0.327HisTyr: 0.327 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
5.232IleAla: 5.232 ± 0.672
0.163IleCys: 0.163 ± 0.124
2.044IleAsp: 2.044 ± 0.387
2.371IleGlu: 2.371 ± 0.425
0.736IlePhe: 0.736 ± 0.245
3.76IleGly: 3.76 ± 0.525
0.163IleHis: 0.163 ± 0.122
0.981IleIle: 0.981 ± 0.27
1.471IleLys: 1.471 ± 0.423
3.352IleLeu: 3.352 ± 0.512
1.063IleMet: 1.063 ± 0.241
0.899IleAsn: 0.899 ± 0.285
1.39IlePro: 1.39 ± 0.392
1.39IleGln: 1.39 ± 0.315
3.106IleArg: 3.106 ± 0.41
1.798IleSer: 1.798 ± 0.328
3.025IleThr: 3.025 ± 0.44
2.044IleVal: 2.044 ± 0.413
0.49IleTrp: 0.49 ± 0.243
0.327IleTyr: 0.327 ± 0.15
0.0IleXaa: 0.0 ± 0.0
Lys
4.823LysAla: 4.823 ± 0.646
0.0LysCys: 0.0 ± 0.0
1.635LysAsp: 1.635 ± 0.395
2.125LysGlu: 2.125 ± 0.372
1.226LysPhe: 1.226 ± 0.294
3.025LysGly: 3.025 ± 0.44
0.736LysHis: 0.736 ± 0.221
0.899LysIle: 0.899 ± 0.251
1.553LysLys: 1.553 ± 0.321
3.188LysLeu: 3.188 ± 0.513
1.308LysMet: 1.308 ± 0.316
1.063LysAsn: 1.063 ± 0.246
2.207LysPro: 2.207 ± 0.599
1.635LysGln: 1.635 ± 0.392
2.044LysArg: 2.044 ± 0.431
2.125LysSer: 2.125 ± 0.385
2.861LysThr: 2.861 ± 0.497
2.534LysVal: 2.534 ± 0.411
0.49LysTrp: 0.49 ± 0.232
0.409LysTyr: 0.409 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
11.117LeuAla: 11.117 ± 0.93
0.899LeuCys: 0.899 ± 0.265
5.313LeuAsp: 5.313 ± 0.908
7.684LeuGlu: 7.684 ± 0.907
2.289LeuPhe: 2.289 ± 0.39
8.093LeuGly: 8.093 ± 1.01
0.654LeuHis: 0.654 ± 0.251
2.861LeuIle: 2.861 ± 0.394
3.433LeuLys: 3.433 ± 0.457
7.929LeuLeu: 7.929 ± 1.136
2.044LeuMet: 2.044 ± 0.464
2.289LeuAsn: 2.289 ± 0.375
5.886LeuPro: 5.886 ± 0.653
3.515LeuGln: 3.515 ± 0.412
8.256LeuArg: 8.256 ± 0.789
4.169LeuSer: 4.169 ± 0.56
4.987LeuThr: 4.987 ± 0.796
8.093LeuVal: 8.093 ± 0.756
1.063LeuTrp: 1.063 ± 0.27
1.226LeuTyr: 1.226 ± 0.302
0.0LeuXaa: 0.0 ± 0.0
Met
2.125MetAla: 2.125 ± 0.36
0.409MetCys: 0.409 ± 0.197
1.962MetAsp: 1.962 ± 0.431
0.736MetGlu: 0.736 ± 0.251
0.327MetPhe: 0.327 ± 0.157
2.861MetGly: 2.861 ± 0.601
0.163MetHis: 0.163 ± 0.112
0.736MetIle: 0.736 ± 0.255
1.144MetLys: 1.144 ± 0.282
1.39MetLeu: 1.39 ± 0.372
0.163MetMet: 0.163 ± 0.134
0.327MetAsn: 0.327 ± 0.173
1.144MetPro: 1.144 ± 0.273
0.327MetGln: 0.327 ± 0.149
1.553MetArg: 1.553 ± 0.321
2.125MetSer: 2.125 ± 0.442
2.534MetThr: 2.534 ± 0.42
1.635MetVal: 1.635 ± 0.361
0.327MetTrp: 0.327 ± 0.156
0.327MetTyr: 0.327 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
1.798AsnAla: 1.798 ± 0.396
0.0AsnCys: 0.0 ± 0.0
1.39AsnAsp: 1.39 ± 0.336
1.144AsnGlu: 1.144 ± 0.294
0.572AsnPhe: 0.572 ± 0.177
3.025AsnGly: 3.025 ± 0.472
0.245AsnHis: 0.245 ± 0.135
1.063AsnIle: 1.063 ± 0.3
1.553AsnLys: 1.553 ± 0.34
2.779AsnLeu: 2.779 ± 0.463
0.736AsnMet: 0.736 ± 0.273
0.654AsnAsn: 0.654 ± 0.192
1.226AsnPro: 1.226 ± 0.353
0.736AsnGln: 0.736 ± 0.199
1.226AsnArg: 1.226 ± 0.34
1.063AsnSer: 1.063 ± 0.267
1.144AsnThr: 1.144 ± 0.289
1.88AsnVal: 1.88 ± 0.445
0.572AsnTrp: 0.572 ± 0.172
0.49AsnTyr: 0.49 ± 0.191
0.0AsnXaa: 0.0 ± 0.0
Pro
9.483ProAla: 9.483 ± 0.932
0.245ProCys: 0.245 ± 0.143
3.842ProAsp: 3.842 ± 0.576
4.333ProGlu: 4.333 ± 0.686
1.308ProPhe: 1.308 ± 0.29
5.395ProGly: 5.395 ± 0.695
0.736ProHis: 0.736 ± 0.264
1.88ProIle: 1.88 ± 0.451
1.553ProLys: 1.553 ± 0.421
4.414ProLeu: 4.414 ± 0.617
1.144ProMet: 1.144 ± 0.284
0.981ProAsn: 0.981 ± 0.308
3.188ProPro: 3.188 ± 0.618
0.736ProGln: 0.736 ± 0.192
3.106ProArg: 3.106 ± 0.479
2.698ProSer: 2.698 ± 0.523
3.515ProThr: 3.515 ± 0.816
5.804ProVal: 5.804 ± 0.614
1.635ProTrp: 1.635 ± 0.292
0.981ProTyr: 0.981 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
3.515GlnAla: 3.515 ± 0.487
0.082GlnCys: 0.082 ± 0.078
1.717GlnAsp: 1.717 ± 0.335
1.962GlnGlu: 1.962 ± 0.417
0.817GlnPhe: 0.817 ± 0.268
1.471GlnGly: 1.471 ± 0.283
0.899GlnHis: 0.899 ± 0.261
1.226GlnIle: 1.226 ± 0.299
1.144GlnLys: 1.144 ± 0.271
4.333GlnLeu: 4.333 ± 0.706
0.409GlnMet: 0.409 ± 0.178
0.981GlnAsn: 0.981 ± 0.242
2.207GlnPro: 2.207 ± 0.507
0.899GlnGln: 0.899 ± 0.294
1.635GlnArg: 1.635 ± 0.342
0.899GlnSer: 0.899 ± 0.312
1.635GlnThr: 1.635 ± 0.513
2.534GlnVal: 2.534 ± 0.613
0.981GlnTrp: 0.981 ± 0.28
0.327GlnTyr: 0.327 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
9.564ArgAla: 9.564 ± 1.027
0.736ArgCys: 0.736 ± 0.208
3.924ArgAsp: 3.924 ± 0.657
3.679ArgGlu: 3.679 ± 0.668
2.534ArgPhe: 2.534 ± 0.365
6.376ArgGly: 6.376 ± 0.601
0.327ArgHis: 0.327 ± 0.203
2.289ArgIle: 2.289 ± 0.399
3.188ArgLys: 3.188 ± 0.465
6.376ArgLeu: 6.376 ± 0.73
1.308ArgMet: 1.308 ± 0.337
1.39ArgAsn: 1.39 ± 0.351
4.006ArgPro: 4.006 ± 0.592
2.534ArgGln: 2.534 ± 0.464
5.722ArgArg: 5.722 ± 0.781
5.15ArgSer: 5.15 ± 0.624
4.823ArgThr: 4.823 ± 0.638
5.64ArgVal: 5.64 ± 0.61
0.899ArgTrp: 0.899 ± 0.314
1.635ArgTyr: 1.635 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
7.602SerAla: 7.602 ± 0.583
0.327SerCys: 0.327 ± 0.163
1.798SerAsp: 1.798 ± 0.515
2.534SerGlu: 2.534 ± 0.482
1.553SerPhe: 1.553 ± 0.317
7.602SerGly: 7.602 ± 0.915
0.981SerHis: 0.981 ± 0.276
2.289SerIle: 2.289 ± 0.407
1.962SerLys: 1.962 ± 0.388
5.15SerLeu: 5.15 ± 0.607
1.471SerMet: 1.471 ± 0.373
1.471SerAsn: 1.471 ± 0.34
2.371SerPro: 2.371 ± 0.367
1.308SerGln: 1.308 ± 0.373
3.679SerArg: 3.679 ± 0.641
4.006SerSer: 4.006 ± 0.682
2.452SerThr: 2.452 ± 0.466
4.251SerVal: 4.251 ± 0.703
1.226SerTrp: 1.226 ± 0.31
0.654SerTyr: 0.654 ± 0.214
0.0SerXaa: 0.0 ± 0.0
Thr
8.256ThrAla: 8.256 ± 0.756
0.245ThrCys: 0.245 ± 0.146
3.27ThrAsp: 3.27 ± 0.485
2.125ThrGlu: 2.125 ± 0.416
1.88ThrPhe: 1.88 ± 0.395
6.213ThrGly: 6.213 ± 0.747
0.245ThrHis: 0.245 ± 0.146
2.779ThrIle: 2.779 ± 0.441
1.88ThrLys: 1.88 ± 0.47
4.251ThrLeu: 4.251 ± 0.516
0.817ThrMet: 0.817 ± 0.24
1.88ThrAsn: 1.88 ± 0.448
3.433ThrPro: 3.433 ± 0.509
1.144ThrGln: 1.144 ± 0.294
2.861ThrArg: 2.861 ± 0.522
2.943ThrSer: 2.943 ± 0.533
4.66ThrThr: 4.66 ± 0.699
6.294ThrVal: 6.294 ± 0.932
0.981ThrTrp: 0.981 ± 0.296
0.981ThrTyr: 0.981 ± 0.354
0.0ThrXaa: 0.0 ± 0.0
Val
8.992ValAla: 8.992 ± 0.956
0.49ValCys: 0.49 ± 0.206
4.66ValAsp: 4.66 ± 0.536
6.294ValGlu: 6.294 ± 0.75
2.044ValPhe: 2.044 ± 0.398
5.967ValGly: 5.967 ± 1.037
1.717ValHis: 1.717 ± 0.386
2.534ValIle: 2.534 ± 0.437
3.433ValLys: 3.433 ± 0.481
7.439ValLeu: 7.439 ± 0.937
1.308ValMet: 1.308 ± 0.327
1.798ValAsn: 1.798 ± 0.374
6.458ValPro: 6.458 ± 0.805
2.452ValGln: 2.452 ± 0.443
6.376ValArg: 6.376 ± 0.917
4.905ValSer: 4.905 ± 0.618
4.823ValThr: 4.823 ± 0.595
7.848ValVal: 7.848 ± 0.868
2.289ValTrp: 2.289 ± 0.378
1.962ValTyr: 1.962 ± 0.367
0.0ValXaa: 0.0 ± 0.0
Trp
2.289TrpAla: 2.289 ± 0.421
0.163TrpCys: 0.163 ± 0.138
1.144TrpAsp: 1.144 ± 0.293
0.654TrpGlu: 0.654 ± 0.289
0.654TrpPhe: 0.654 ± 0.237
1.308TrpGly: 1.308 ± 0.345
0.327TrpHis: 0.327 ± 0.144
0.736TrpIle: 0.736 ± 0.352
0.409TrpLys: 0.409 ± 0.146
2.125TrpLeu: 2.125 ± 0.411
0.49TrpMet: 0.49 ± 0.201
0.899TrpAsn: 0.899 ± 0.292
1.717TrpPro: 1.717 ± 0.313
1.308TrpGln: 1.308 ± 0.273
1.717TrpArg: 1.717 ± 0.419
1.553TrpSer: 1.553 ± 0.374
1.553TrpThr: 1.553 ± 0.423
1.39TrpVal: 1.39 ± 0.331
0.572TrpTrp: 0.572 ± 0.209
0.572TrpTyr: 0.572 ± 0.281
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.371TyrAla: 2.371 ± 0.57
0.409TyrCys: 0.409 ± 0.177
1.471TyrAsp: 1.471 ± 0.282
1.226TyrGlu: 1.226 ± 0.356
0.49TyrPhe: 0.49 ± 0.192
1.962TyrGly: 1.962 ± 0.464
0.0TyrHis: 0.0 ± 0.0
0.409TyrIle: 0.409 ± 0.19
0.409TyrLys: 0.409 ± 0.175
2.779TyrLeu: 2.779 ± 0.539
0.327TyrMet: 0.327 ± 0.213
0.327TyrAsn: 0.327 ± 0.155
0.736TyrPro: 0.736 ± 0.277
0.245TyrGln: 0.245 ± 0.138
1.798TyrArg: 1.798 ± 0.358
1.798TyrSer: 1.798 ± 0.402
0.409TyrThr: 0.409 ± 0.179
1.471TyrVal: 1.471 ± 0.3
0.163TyrTrp: 0.163 ± 0.109
0.163TyrTyr: 0.163 ± 0.118
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (12234 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski