Acinetobacter phage AM106
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A499Q7R7|A0A499Q7R7_9CAUD Uncharacterized protein OS=Acinetobacter phage AM106 OX=2163986 GN=AM106_45 PE=4 SV=1
MM1 pKa = 7.37 VNTEE5 pKa = 5.06 YY6 pKa = 11.1 VPEE9 pKa = 4.28 WYY11 pKa = 7.87 ITPFQHH17 pKa = 5.72 VQYY20 pKa = 10.04 TLARR24 pKa = 11.84 NQLHH28 pKa = 6.45 MDD30 pKa = 4.46 LLFEE34 pKa = 5.81 DD35 pKa = 4.49 MDD37 pKa = 4.21 EE38 pKa = 4.61 ADD40 pKa = 3.51 QFLDD44 pKa = 3.35 MGADD48 pKa = 3.5 AQVSTFSDD56 pKa = 3.47 GAYY59 pKa = 10.26 AIVQIGDD66 pKa = 3.52 TADD69 pKa = 3.25 KK70 pKa = 11.21 DD71 pKa = 3.87 KK72 pKa = 11.17 IQVYY76 pKa = 10.53 GLLLHH81 pKa = 6.42 EE82 pKa = 5.33 AVHH85 pKa = 5.92 VWQIVKK91 pKa = 10.35 KK92 pKa = 10.43 RR93 pKa = 11.84 MGEE96 pKa = 4.06 SEE98 pKa = 4.03 PSVEE102 pKa = 4.16 FEE104 pKa = 4.56 AYY106 pKa = 10.09 SIQAIAQDD114 pKa = 3.97 LFEE117 pKa = 4.76 MYY119 pKa = 9.14 EE120 pKa = 3.87 ASEE123 pKa = 4.31 VSNGMEE129 pKa = 4.71 GEE131 pKa = 4.2 KK132 pKa = 10.63 ADD134 pKa = 3.7
Molecular weight: 15.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.998
IPC2_protein 4.075
IPC_protein 4.012
Toseland 3.834
ProMoST 4.101
Dawson 3.986
Bjellqvist 4.164
Wikipedia 3.884
Rodwell 3.846
Grimsley 3.745
Solomon 3.973
Lehninger 3.923
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.126
Patrickios 2.791
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.041
Protein with the highest isoelectric point:
>tr|A0A499Q7P4|A0A499Q7P4_9CAUD Uncharacterized protein OS=Acinetobacter phage AM106 OX=2163986 GN=AM106_04 PE=4 SV=1
MM1 pKa = 7.08 THH3 pKa = 6.78 FKK5 pKa = 10.28 KK6 pKa = 10.2 HH7 pKa = 5.75 PDD9 pKa = 3.37 GYY11 pKa = 11.13 KK12 pKa = 10.38 SFLGRR17 pKa = 11.84 DD18 pKa = 3.51 DD19 pKa = 3.9 KK20 pKa = 11.62 GLYY23 pKa = 8.82 SVRR26 pKa = 11.84 IGWQVYY32 pKa = 9.57 ASNANGSVLYY42 pKa = 10.14 KK43 pKa = 10.87 VKK45 pKa = 10.91 DD46 pKa = 3.8 GVKK49 pKa = 9.28 TPLNVFRR56 pKa = 11.84 FRR58 pKa = 11.84 TSYY61 pKa = 9.84 PKK63 pKa = 10.28 VWNEE67 pKa = 3.36 LTQEE71 pKa = 3.45 IDD73 pKa = 3.47 FQRR76 pKa = 11.84 RR77 pKa = 11.84 KK78 pKa = 9.79 QLAIKK83 pKa = 10.26 LRR85 pKa = 11.84 EE86 pKa = 4.24 TNIPTYY92 pKa = 10.38 DD93 pKa = 3.01 RR94 pKa = 11.84 KK95 pKa = 10.61 AYY97 pKa = 7.93 KK98 pKa = 9.58 QKK100 pKa = 10.84 RR101 pKa = 11.84 GFTGSRR107 pKa = 3.24
Molecular weight: 12.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.246
IPC2_protein 9.794
IPC_protein 10.087
Toseland 10.467
ProMoST 10.175
Dawson 10.599
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 11.111
Grimsley 10.657
Solomon 10.643
Lehninger 10.613
Nozaki 10.423
DTASelect 10.248
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.511
Patrickios 10.818
IPC_peptide 10.643
IPC2_peptide 8.843
IPC2.peptide.svr19 8.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
16009
38
3489
228.7
25.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.207 ± 0.548
0.825 ± 0.162
5.484 ± 0.216
6.696 ± 0.632
3.573 ± 0.306
6.615 ± 0.301
1.518 ± 0.248
6.109 ± 0.217
7.09 ± 0.628
8.008 ± 0.319
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.257
5.722 ± 0.476
3.448 ± 0.246
4.722 ± 0.271
4.216 ± 0.257
6.852 ± 0.689
6.59 ± 0.748
6.353 ± 0.18
1.306 ± 0.128
3.411 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here