Amino acid dipepetide frequency for Acinetobacter phage AM106

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.183AlaAla: 9.183 ± 1.292
1.0AlaCys: 1.0 ± 0.312
4.998AlaAsp: 4.998 ± 0.617
6.372AlaGlu: 6.372 ± 0.777
2.874AlaPhe: 2.874 ± 0.393
5.435AlaGly: 5.435 ± 0.772
1.437AlaHis: 1.437 ± 0.324
6.309AlaIle: 6.309 ± 0.857
5.997AlaLys: 5.997 ± 0.542
7.746AlaLeu: 7.746 ± 0.833
2.374AlaMet: 2.374 ± 0.65
5.935AlaAsn: 5.935 ± 1.017
2.561AlaPro: 2.561 ± 0.407
4.435AlaGln: 4.435 ± 0.752
3.686AlaArg: 3.686 ± 0.559
5.935AlaSer: 5.935 ± 1.184
5.935AlaThr: 5.935 ± 1.101
5.56AlaVal: 5.56 ± 0.569
1.374AlaTrp: 1.374 ± 0.366
2.874AlaTyr: 2.874 ± 0.41
0.0AlaXaa: 0.0 ± 0.0
Cys
0.437CysAla: 0.437 ± 0.15
0.125CysCys: 0.125 ± 0.112
0.437CysAsp: 0.437 ± 0.152
0.937CysGlu: 0.937 ± 0.339
0.437CysPhe: 0.437 ± 0.194
1.0CysGly: 1.0 ± 0.304
0.25CysHis: 0.25 ± 0.144
0.562CysIle: 0.562 ± 0.206
0.5CysLys: 0.5 ± 0.215
0.562CysLeu: 0.562 ± 0.228
0.25CysMet: 0.25 ± 0.118
0.312CysAsn: 0.312 ± 0.148
0.062CysPro: 0.062 ± 0.069
0.25CysGln: 0.25 ± 0.141
0.625CysArg: 0.625 ± 0.233
0.187CysSer: 0.187 ± 0.091
0.5CysThr: 0.5 ± 0.149
0.375CysVal: 0.375 ± 0.18
0.062CysTrp: 0.062 ± 0.061
0.375CysTyr: 0.375 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
6.059AspAla: 6.059 ± 0.814
0.312AspCys: 0.312 ± 0.162
2.374AspAsp: 2.374 ± 0.537
3.373AspGlu: 3.373 ± 0.437
3.123AspPhe: 3.123 ± 0.609
5.372AspGly: 5.372 ± 0.579
0.625AspHis: 0.625 ± 0.297
2.686AspIle: 2.686 ± 0.427
3.811AspLys: 3.811 ± 0.573
4.748AspLeu: 4.748 ± 0.522
1.249AspMet: 1.249 ± 0.31
1.874AspAsn: 1.874 ± 0.34
1.812AspPro: 1.812 ± 0.376
3.123AspGln: 3.123 ± 0.553
1.187AspArg: 1.187 ± 0.244
3.998AspSer: 3.998 ± 0.588
2.499AspThr: 2.499 ± 0.409
3.873AspVal: 3.873 ± 0.452
1.0AspTrp: 1.0 ± 0.342
1.749AspTyr: 1.749 ± 0.401
0.0AspXaa: 0.0 ± 0.0
Glu
6.247GluAla: 6.247 ± 0.967
0.687GluCys: 0.687 ± 0.269
3.186GluAsp: 3.186 ± 0.526
4.373GluGlu: 4.373 ± 1.063
3.061GluPhe: 3.061 ± 0.559
4.123GluGly: 4.123 ± 0.576
1.374GluHis: 1.374 ± 0.411
5.122GluIle: 5.122 ± 0.551
5.122GluLys: 5.122 ± 1.174
6.809GluLeu: 6.809 ± 1.091
1.249GluMet: 1.249 ± 0.33
3.498GluAsn: 3.498 ± 0.631
2.249GluPro: 2.249 ± 0.508
3.561GluGln: 3.561 ± 0.842
2.811GluArg: 2.811 ± 0.403
3.061GluSer: 3.061 ± 0.59
2.124GluThr: 2.124 ± 0.401
4.56GluVal: 4.56 ± 0.68
1.0GluTrp: 1.0 ± 0.242
2.749GluTyr: 2.749 ± 0.515
0.0GluXaa: 0.0 ± 0.0
Phe
2.499PheAla: 2.499 ± 0.334
0.25PheCys: 0.25 ± 0.175
2.186PheAsp: 2.186 ± 0.471
3.436PheGlu: 3.436 ± 0.613
1.437PhePhe: 1.437 ± 0.329
2.499PheGly: 2.499 ± 0.488
0.687PheHis: 0.687 ± 0.228
2.124PheIle: 2.124 ± 0.398
2.936PheLys: 2.936 ± 0.505
1.749PheLeu: 1.749 ± 0.455
0.687PheMet: 0.687 ± 0.19
2.311PheAsn: 2.311 ± 0.362
1.124PhePro: 1.124 ± 0.346
1.312PheGln: 1.312 ± 0.353
1.437PheArg: 1.437 ± 0.256
1.624PheSer: 1.624 ± 0.332
2.999PheThr: 2.999 ± 0.441
2.311PheVal: 2.311 ± 0.369
0.562PheTrp: 0.562 ± 0.166
1.562PheTyr: 1.562 ± 0.282
0.0PheXaa: 0.0 ± 0.0
Gly
4.873GlyAla: 4.873 ± 0.569
0.5GlyCys: 0.5 ± 0.154
3.311GlyAsp: 3.311 ± 0.554
4.373GlyGlu: 4.373 ± 0.644
2.811GlyPhe: 2.811 ± 0.567
6.184GlyGly: 6.184 ± 0.84
1.249GlyHis: 1.249 ± 0.255
3.123GlyIle: 3.123 ± 0.404
5.06GlyLys: 5.06 ± 0.58
5.06GlyLeu: 5.06 ± 0.603
1.812GlyMet: 1.812 ± 0.302
4.06GlyAsn: 4.06 ± 0.91
1.812GlyPro: 1.812 ± 0.38
2.874GlyGln: 2.874 ± 0.342
3.373GlyArg: 3.373 ± 0.486
3.311GlySer: 3.311 ± 0.47
4.373GlyThr: 4.373 ± 1.133
4.435GlyVal: 4.435 ± 0.56
1.374GlyTrp: 1.374 ± 0.385
2.186GlyTyr: 2.186 ± 0.336
0.0GlyXaa: 0.0 ± 0.0
His
1.562HisAla: 1.562 ± 0.419
0.187HisCys: 0.187 ± 0.122
0.687HisAsp: 0.687 ± 0.342
0.875HisGlu: 0.875 ± 0.324
0.75HisPhe: 0.75 ± 0.208
0.687HisGly: 0.687 ± 0.208
0.5HisHis: 0.5 ± 0.204
1.062HisIle: 1.062 ± 0.269
0.937HisLys: 0.937 ± 0.293
1.687HisLeu: 1.687 ± 0.451
0.562HisMet: 0.562 ± 0.257
0.312HisAsn: 0.312 ± 0.124
0.937HisPro: 0.937 ± 0.432
0.687HisGln: 0.687 ± 0.233
0.5HisArg: 0.5 ± 0.193
0.875HisSer: 0.875 ± 0.224
0.437HisThr: 0.437 ± 0.132
0.875HisVal: 0.875 ± 0.28
0.375HisTrp: 0.375 ± 0.134
0.687HisTyr: 0.687 ± 0.235
0.0HisXaa: 0.0 ± 0.0
Ile
5.31IleAla: 5.31 ± 0.562
0.687IleCys: 0.687 ± 0.174
4.748IleAsp: 4.748 ± 0.606
5.06IleGlu: 5.06 ± 0.517
0.875IlePhe: 0.875 ± 0.229
3.873IleGly: 3.873 ± 0.48
1.0IleHis: 1.0 ± 0.313
2.624IleIle: 2.624 ± 0.509
3.623IleLys: 3.623 ± 0.529
3.311IleLeu: 3.311 ± 0.493
0.875IleMet: 0.875 ± 0.327
3.998IleAsn: 3.998 ± 0.556
2.811IlePro: 2.811 ± 0.41
3.373IleGln: 3.373 ± 0.49
2.249IleArg: 2.249 ± 0.345
4.685IleSer: 4.685 ± 0.714
6.122IleThr: 6.122 ± 0.979
3.561IleVal: 3.561 ± 0.502
0.5IleTrp: 0.5 ± 0.174
1.812IleTyr: 1.812 ± 0.421
0.0IleXaa: 0.0 ± 0.0
Lys
7.246LysAla: 7.246 ± 1.035
0.187LysCys: 0.187 ± 0.085
3.686LysAsp: 3.686 ± 0.651
4.623LysGlu: 4.623 ± 1.16
2.811LysPhe: 2.811 ± 0.552
3.748LysGly: 3.748 ± 0.578
1.437LysHis: 1.437 ± 0.367
3.811LysIle: 3.811 ± 0.453
5.497LysLys: 5.497 ± 0.989
5.56LysLeu: 5.56 ± 0.622
1.437LysMet: 1.437 ± 0.271
2.686LysAsn: 2.686 ± 0.582
2.999LysPro: 2.999 ± 0.542
3.998LysGln: 3.998 ± 0.778
3.623LysArg: 3.623 ± 0.629
4.373LysSer: 4.373 ± 0.505
4.685LysThr: 4.685 ± 0.613
4.435LysVal: 4.435 ± 0.411
1.0LysTrp: 1.0 ± 0.274
3.061LysTyr: 3.061 ± 0.576
0.0LysXaa: 0.0 ± 0.0
Leu
7.871LeuAla: 7.871 ± 0.767
0.687LeuCys: 0.687 ± 0.24
5.747LeuAsp: 5.747 ± 0.597
6.247LeuGlu: 6.247 ± 1.028
1.999LeuPhe: 1.999 ± 0.364
4.998LeuGly: 4.998 ± 0.586
0.875LeuHis: 0.875 ± 0.257
4.748LeuIle: 4.748 ± 0.733
6.559LeuLys: 6.559 ± 1.008
5.56LeuLeu: 5.56 ± 0.652
1.062LeuMet: 1.062 ± 0.256
6.122LeuAsn: 6.122 ± 0.507
2.499LeuPro: 2.499 ± 0.394
4.31LeuGln: 4.31 ± 0.472
3.436LeuArg: 3.436 ± 0.592
5.247LeuSer: 5.247 ± 0.632
5.622LeuThr: 5.622 ± 0.679
3.873LeuVal: 3.873 ± 0.539
0.562LeuTrp: 0.562 ± 0.232
2.061LeuTyr: 2.061 ± 0.352
0.0LeuXaa: 0.0 ± 0.0
Met
2.186MetAla: 2.186 ± 0.462
0.125MetCys: 0.125 ± 0.101
1.312MetAsp: 1.312 ± 0.395
0.875MetGlu: 0.875 ± 0.356
0.937MetPhe: 0.937 ± 0.226
1.437MetGly: 1.437 ± 0.313
0.312MetHis: 0.312 ± 0.126
1.562MetIle: 1.562 ± 0.276
1.687MetLys: 1.687 ± 0.391
2.374MetLeu: 2.374 ± 0.585
0.812MetMet: 0.812 ± 0.293
1.249MetAsn: 1.249 ± 0.298
1.062MetPro: 1.062 ± 0.287
0.687MetGln: 0.687 ± 0.195
1.0MetArg: 1.0 ± 0.293
1.562MetSer: 1.562 ± 0.356
1.812MetThr: 1.812 ± 0.457
0.875MetVal: 0.875 ± 0.173
0.062MetTrp: 0.062 ± 0.061
0.625MetTyr: 0.625 ± 0.215
0.0MetXaa: 0.0 ± 0.0
Asn
5.81AsnAla: 5.81 ± 1.434
0.25AsnCys: 0.25 ± 0.139
2.686AsnAsp: 2.686 ± 0.468
2.749AsnGlu: 2.749 ± 0.57
1.624AsnPhe: 1.624 ± 0.392
4.498AsnGly: 4.498 ± 0.776
0.625AsnHis: 0.625 ± 0.269
3.186AsnIle: 3.186 ± 0.71
3.561AsnLys: 3.561 ± 0.429
4.873AsnLeu: 4.873 ± 0.655
1.312AsnMet: 1.312 ± 0.395
3.436AsnAsn: 3.436 ± 0.89
2.624AsnPro: 2.624 ± 0.507
2.499AsnGln: 2.499 ± 0.376
2.186AsnArg: 2.186 ± 0.378
4.998AsnSer: 4.998 ± 1.064
4.06AsnThr: 4.06 ± 0.928
3.873AsnVal: 3.873 ± 0.498
0.812AsnTrp: 0.812 ± 0.235
1.562AsnTyr: 1.562 ± 0.387
0.0AsnXaa: 0.0 ± 0.0
Pro
2.686ProAla: 2.686 ± 0.373
0.312ProCys: 0.312 ± 0.182
2.374ProAsp: 2.374 ± 0.433
2.436ProGlu: 2.436 ± 0.575
1.249ProPhe: 1.249 ± 0.298
1.187ProGly: 1.187 ± 0.261
0.75ProHis: 0.75 ± 0.219
2.061ProIle: 2.061 ± 0.307
2.874ProLys: 2.874 ± 0.483
2.811ProLeu: 2.811 ± 0.542
0.875ProMet: 0.875 ± 0.237
2.124ProAsn: 2.124 ± 0.432
1.562ProPro: 1.562 ± 0.361
1.062ProGln: 1.062 ± 0.25
1.187ProArg: 1.187 ± 0.262
3.061ProSer: 3.061 ± 0.31
2.061ProThr: 2.061 ± 0.396
2.499ProVal: 2.499 ± 0.364
0.437ProTrp: 0.437 ± 0.149
0.875ProTyr: 0.875 ± 0.214
0.0ProXaa: 0.0 ± 0.0
Gln
4.998GlnAla: 4.998 ± 0.858
0.375GlnCys: 0.375 ± 0.141
1.999GlnAsp: 1.999 ± 0.307
3.748GlnGlu: 3.748 ± 0.619
1.749GlnPhe: 1.749 ± 0.287
2.686GlnGly: 2.686 ± 0.599
0.437GlnHis: 0.437 ± 0.149
2.811GlnIle: 2.811 ± 0.529
3.623GlnLys: 3.623 ± 0.723
3.811GlnLeu: 3.811 ± 0.646
1.249GlnMet: 1.249 ± 0.351
2.499GlnAsn: 2.499 ± 0.373
0.687GlnPro: 0.687 ± 0.19
2.499GlnGln: 2.499 ± 0.416
2.186GlnArg: 2.186 ± 0.443
2.999GlnSer: 2.999 ± 0.65
3.248GlnThr: 3.248 ± 0.37
2.936GlnVal: 2.936 ± 0.451
0.5GlnTrp: 0.5 ± 0.169
2.186GlnTyr: 2.186 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
3.061ArgAla: 3.061 ± 0.541
0.437ArgCys: 0.437 ± 0.207
2.124ArgAsp: 2.124 ± 0.402
2.811ArgGlu: 2.811 ± 0.618
1.499ArgPhe: 1.499 ± 0.343
2.186ArgGly: 2.186 ± 0.423
0.687ArgHis: 0.687 ± 0.254
3.248ArgIle: 3.248 ± 0.382
2.436ArgLys: 2.436 ± 0.465
4.498ArgLeu: 4.498 ± 0.44
1.312ArgMet: 1.312 ± 0.338
2.124ArgAsn: 2.124 ± 0.389
1.249ArgPro: 1.249 ± 0.32
2.561ArgGln: 2.561 ± 0.535
1.749ArgArg: 1.749 ± 0.335
2.374ArgSer: 2.374 ± 0.499
2.749ArgThr: 2.749 ± 0.477
2.936ArgVal: 2.936 ± 0.738
0.375ArgTrp: 0.375 ± 0.152
1.749ArgTyr: 1.749 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
7.184SerAla: 7.184 ± 1.849
0.562SerCys: 0.562 ± 0.226
3.373SerAsp: 3.373 ± 0.47
4.623SerGlu: 4.623 ± 0.764
2.311SerPhe: 2.311 ± 0.496
5.247SerGly: 5.247 ± 0.829
0.937SerHis: 0.937 ± 0.272
4.685SerIle: 4.685 ± 1.212
4.873SerLys: 4.873 ± 0.573
5.81SerLeu: 5.81 ± 0.779
1.312SerMet: 1.312 ± 0.321
4.56SerAsn: 4.56 ± 0.954
1.874SerPro: 1.874 ± 0.36
2.936SerGln: 2.936 ± 0.637
2.436SerArg: 2.436 ± 0.279
4.935SerSer: 4.935 ± 1.121
3.811SerThr: 3.811 ± 1.082
4.435SerVal: 4.435 ± 0.491
0.812SerTrp: 0.812 ± 0.158
1.812SerTyr: 1.812 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
6.809ThrAla: 6.809 ± 1.562
0.25ThrCys: 0.25 ± 0.095
2.811ThrAsp: 2.811 ± 0.407
3.248ThrGlu: 3.248 ± 0.45
2.561ThrPhe: 2.561 ± 0.459
4.185ThrGly: 4.185 ± 0.547
0.812ThrHis: 0.812 ± 0.35
4.185ThrIle: 4.185 ± 0.569
4.685ThrLys: 4.685 ± 0.712
4.685ThrLeu: 4.685 ± 0.457
1.0ThrMet: 1.0 ± 0.253
4.185ThrAsn: 4.185 ± 1.644
2.686ThrPro: 2.686 ± 0.338
2.249ThrGln: 2.249 ± 0.297
2.811ThrArg: 2.811 ± 0.436
5.935ThrSer: 5.935 ± 1.942
4.873ThrThr: 4.873 ± 1.103
4.873ThrVal: 4.873 ± 0.574
0.625ThrTrp: 0.625 ± 0.138
2.436ThrTyr: 2.436 ± 0.399
0.0ThrXaa: 0.0 ± 0.0
Val
4.623ValAla: 4.623 ± 0.513
0.687ValCys: 0.687 ± 0.32
3.873ValAsp: 3.873 ± 0.516
3.748ValGlu: 3.748 ± 0.506
1.749ValPhe: 1.749 ± 0.371
4.06ValGly: 4.06 ± 0.548
0.375ValHis: 0.375 ± 0.154
4.435ValIle: 4.435 ± 0.52
4.623ValLys: 4.623 ± 0.698
4.06ValLeu: 4.06 ± 0.536
1.437ValMet: 1.437 ± 0.457
4.06ValAsn: 4.06 ± 0.49
2.436ValPro: 2.436 ± 0.404
2.624ValGln: 2.624 ± 0.551
2.686ValArg: 2.686 ± 0.47
5.185ValSer: 5.185 ± 0.867
5.435ValThr: 5.435 ± 0.868
4.435ValVal: 4.435 ± 0.748
0.812ValTrp: 0.812 ± 0.182
2.186ValTyr: 2.186 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
1.0TrpAla: 1.0 ± 0.307
0.375TrpCys: 0.375 ± 0.138
0.625TrpAsp: 0.625 ± 0.142
0.625TrpGlu: 0.625 ± 0.176
0.562TrpPhe: 0.562 ± 0.156
0.687TrpGly: 0.687 ± 0.249
0.25TrpHis: 0.25 ± 0.109
0.812TrpIle: 0.812 ± 0.257
0.625TrpLys: 0.625 ± 0.213
1.312TrpLeu: 1.312 ± 0.304
0.625TrpMet: 0.625 ± 0.18
0.437TrpAsn: 0.437 ± 0.18
0.125TrpPro: 0.125 ± 0.095
0.75TrpGln: 0.75 ± 0.199
0.625TrpArg: 0.625 ± 0.203
1.312TrpSer: 1.312 ± 0.257
0.937TrpThr: 0.937 ± 0.283
0.625TrpVal: 0.625 ± 0.182
0.25TrpTrp: 0.25 ± 0.123
0.5TrpTyr: 0.5 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.436TyrAla: 2.436 ± 0.421
0.25TyrCys: 0.25 ± 0.129
2.311TyrAsp: 2.311 ± 0.391
2.436TyrGlu: 2.436 ± 0.466
1.312TyrPhe: 1.312 ± 0.286
2.061TyrGly: 2.061 ± 0.477
0.562TyrHis: 0.562 ± 0.22
1.937TyrIle: 1.937 ± 0.309
1.874TyrLys: 1.874 ± 0.394
3.061TyrLeu: 3.061 ± 0.482
1.0TyrMet: 1.0 ± 0.263
1.437TyrAsn: 1.437 ± 0.276
1.312TyrPro: 1.312 ± 0.261
1.437TyrGln: 1.437 ± 0.369
2.374TyrArg: 2.374 ± 0.427
2.874TyrSer: 2.874 ± 0.455
1.624TyrThr: 1.624 ± 0.299
2.186TyrVal: 2.186 ± 0.284
0.562TyrTrp: 0.562 ± 0.21
1.062TyrTyr: 1.062 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (16009 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski