Qipengyuania marisflavi
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2508 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S3P2T8|A0A5S3P2T8_9SPHN S9 family peptidase OS=Qipengyuania marisflavi OX=2486356 GN=FEV51_10385 PE=4 SV=1
MM1 pKa = 7.78 SYY3 pKa = 11.05 LFDD6 pKa = 3.33 QRR8 pKa = 11.84 SFQFNRR14 pKa = 11.84 FDD16 pKa = 5.23 ADD18 pKa = 2.95 MGLNFGRR25 pKa = 11.84 AGSRR29 pKa = 11.84 AAMWSDD35 pKa = 3.33 VGVSHH40 pKa = 7.69 HH41 pKa = 7.47 DD42 pKa = 3.33 GHH44 pKa = 7.65 DD45 pKa = 3.6 HH46 pKa = 7.28 FKK48 pKa = 8.34 MTASLFADD56 pKa = 3.86 STSSQAPVDD65 pKa = 4.08 LAADD69 pKa = 3.76 VPGDD73 pKa = 3.42 SGTGVTVTIGDD84 pKa = 3.7 MVYY87 pKa = 11.46 DD88 pKa = 3.94 MLEE91 pKa = 3.93 VAGDD95 pKa = 3.94 HH96 pKa = 6.56 DD97 pKa = 4.08 WFRR100 pKa = 11.84 IDD102 pKa = 4.1 LAAGQTLSIVLTGYY116 pKa = 11.23 GDD118 pKa = 4.35 DD119 pKa = 4.47 SLIDD123 pKa = 3.85 PYY125 pKa = 11.4 LYY127 pKa = 10.95 LRR129 pKa = 11.84 DD130 pKa = 3.55 ASGNLVAEE138 pKa = 4.7 NDD140 pKa = 3.93 DD141 pKa = 3.71 VTPGSDD147 pKa = 2.78 RR148 pKa = 11.84 NSRR151 pKa = 11.84 IDD153 pKa = 4.26 FEE155 pKa = 4.36 ATTAGTYY162 pKa = 10.51 YY163 pKa = 10.05 IDD165 pKa = 3.2 VGAWDD170 pKa = 3.74 EE171 pKa = 4.99 GYY173 pKa = 11.1 AGDD176 pKa = 3.93 YY177 pKa = 9.83 QLQIFDD183 pKa = 4.72 SEE185 pKa = 4.42 ATTDD189 pKa = 4.32 AITANASTTASMLVGAPFHH208 pKa = 6.53 GVIEE212 pKa = 4.54 TPSDD216 pKa = 3.78 HH217 pKa = 7.32 DD218 pKa = 3.5 WVAIEE223 pKa = 4.55 LVAGTEE229 pKa = 4.19 YY230 pKa = 10.84 QVDD233 pKa = 4.07 LSGAGPNALNNPYY246 pKa = 10.55 LIVRR250 pKa = 11.84 DD251 pKa = 3.44 AAGNIVAEE259 pKa = 4.16 NDD261 pKa = 3.28 NTNGLDD267 pKa = 3.53 SAVTFTATTSGTYY280 pKa = 10.62 YY281 pKa = 10.0 IDD283 pKa = 3.53 ASAAFSGGTGEE294 pKa = 4.16 YY295 pKa = 9.7 LIEE298 pKa = 4.24 VTPPLPIFTFDD309 pKa = 3.91 QIANQLTNTFWGGEE323 pKa = 3.61 QHH325 pKa = 6.72 AFVVGADD332 pKa = 3.4 NSLTVNLTGINADD345 pKa = 3.57 GQGLARR351 pKa = 11.84 AALQSWTDD359 pKa = 3.13 VTGIQFVEE367 pKa = 4.3 TNGAAEE373 pKa = 4.21 ITFQDD378 pKa = 3.86 HH379 pKa = 6.22 EE380 pKa = 4.81 SGAFASSTYY389 pKa = 9.71 NGSGAIVSSTVNVSTQWIANYY410 pKa = 7.94 GTALDD415 pKa = 4.62 DD416 pKa = 3.68 YY417 pKa = 11.06 SYY419 pKa = 10.16 QTFIHH424 pKa = 6.78 EE425 pKa = 4.35 IGHH428 pKa = 6.36 ALGLGHH434 pKa = 7.13 GGNYY438 pKa = 9.74 NGSAEE443 pKa = 4.26 YY444 pKa = 10.01 PDD446 pKa = 4.38 DD447 pKa = 3.5 ATYY450 pKa = 11.25 QNDD453 pKa = 3.06 SWATTVMSYY462 pKa = 10.2 FSQTEE467 pKa = 3.36 NDD469 pKa = 3.5 YY470 pKa = 11.12 FADD473 pKa = 3.42 QGFTRR478 pKa = 11.84 ASVTTPMNADD488 pKa = 3.24 VVAMRR493 pKa = 11.84 VLYY496 pKa = 10.47 GLSSDD501 pKa = 3.39 TRR503 pKa = 11.84 LGNTTYY509 pKa = 11.15 GFNSNAGSTAFDD521 pKa = 2.97 ATVFNDD527 pKa = 3.49 TAYY530 pKa = 9.9 TIVDD534 pKa = 3.41 SGGIDD539 pKa = 3.24 TLDD542 pKa = 3.17 YY543 pKa = 11.52 SGFSQDD549 pKa = 3.26 QLIDD553 pKa = 3.62 LRR555 pKa = 11.84 PEE557 pKa = 3.62 YY558 pKa = 10.83 FMNIGPAIGNVMIGRR573 pKa = 11.84 GTFIEE578 pKa = 4.14 NAIGGSGADD587 pKa = 4.07 TINGNQFNNALTGWAGADD605 pKa = 3.58 VISGFGGRR613 pKa = 11.84 DD614 pKa = 3.18 TLQGGNGNDD623 pKa = 3.64 TLNGGQHH630 pKa = 7.12 LDD632 pKa = 3.37 TVYY635 pKa = 11.2 GGGGADD641 pKa = 4.27 AINGGGANDD650 pKa = 4.08 LLYY653 pKa = 11.31 GEE655 pKa = 5.41 GGNDD659 pKa = 3.7 TILGANGNDD668 pKa = 4.08 YY669 pKa = 11.07 IDD671 pKa = 4.41 GGAGNDD677 pKa = 4.01 TINGGVGSDD686 pKa = 4.02 EE687 pKa = 4.19 IFGGDD692 pKa = 3.67 GDD694 pKa = 4.1 DD695 pKa = 4.48 TISGGHH701 pKa = 6.13 GLDD704 pKa = 4.07 VIHH707 pKa = 7.09 GGLGEE712 pKa = 4.01 DD713 pKa = 4.42 TINGGSFADD722 pKa = 4.42 TIFGDD727 pKa = 3.87 EE728 pKa = 4.78 GNDD731 pKa = 3.57 TLLGGPGSDD740 pKa = 3.83 TIYY743 pKa = 11.11 GGADD747 pKa = 3.19 NDD749 pKa = 4.74 SISGGNANDD758 pKa = 3.76 TLYY761 pKa = 11.44 GDD763 pKa = 4.87 GGNDD767 pKa = 3.71 TINGNNGADD776 pKa = 4.0 TIDD779 pKa = 3.73 GGAGIDD785 pKa = 3.79 RR786 pKa = 11.84 ISGGLAADD794 pKa = 3.71 ILTGGGGNDD803 pKa = 3.29 LFVFDD808 pKa = 5.1 SLLGGPNIDD817 pKa = 4.19 QITDD821 pKa = 3.75 FTSGSDD827 pKa = 3.64 TINLDD832 pKa = 3.34 RR833 pKa = 11.84 AIFTGIGSDD842 pKa = 3.53 GALAGSAFRR851 pKa = 11.84 VGTAAQDD858 pKa = 3.27 ADD860 pKa = 3.69 DD861 pKa = 5.11 RR862 pKa = 11.84 IIYY865 pKa = 7.89 TRR867 pKa = 11.84 STGEE871 pKa = 3.45 IFYY874 pKa = 10.88 DD875 pKa = 3.57 ADD877 pKa = 3.83 GVGGADD883 pKa = 2.64 AVLFAKK889 pKa = 10.29 VDD891 pKa = 3.33 AGMVLSASDD900 pKa = 3.81 FAAFSASAAAGSGAKK915 pKa = 10.46 GGDD918 pKa = 3.2 AMFAPLEE925 pKa = 4.25 SLHH928 pKa = 6.54 GVDD931 pKa = 4.53 IFAIVV936 pKa = 2.83
Molecular weight: 96.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.541
IPC_protein 3.605
Toseland 3.363
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.77
Wikipedia 3.592
Rodwell 3.427
Grimsley 3.261
Solomon 3.617
Lehninger 3.579
Nozaki 3.732
DTASelect 4.05
Thurlkill 3.427
EMBOSS 3.592
Sillero 3.732
Patrickios 0.617
IPC_peptide 3.617
IPC2_peptide 3.706
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A5S3P3F6|A0A5S3P3F6_9SPHN DUF969 domain-containing protein OS=Qipengyuania marisflavi OX=2486356 GN=FEV51_09430 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 7.9 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.78 KK41 pKa = 10.52 LCAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2508
0
2508
813454
39
5844
324.3
35.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.014 ± 0.082
0.825 ± 0.016
6.261 ± 0.043
5.957 ± 0.056
3.617 ± 0.029
8.878 ± 0.083
1.993 ± 0.027
5.009 ± 0.032
3.207 ± 0.048
9.833 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.587 ± 0.028
2.563 ± 0.034
5.052 ± 0.042
3.304 ± 0.027
6.946 ± 0.057
5.265 ± 0.044
5.212 ± 0.05
6.898 ± 0.037
1.421 ± 0.025
2.159 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here