Leuconostoc lactis
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1614 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q0YI61|A0A0Q0YI61_LEULA SPFH/Band 7/PHB domain protein OS=Leuconostoc lactis OX=1246 GN=FGL80_07535 PE=4 SV=1
MM1 pKa = 7.14 KK2 pKa = 10.07 AAVISFPGSNCDD14 pKa = 3.79 FDD16 pKa = 4.26 MLYY19 pKa = 10.95 ALQDD23 pKa = 3.82 FGVDD27 pKa = 3.44 AQIISAKK34 pKa = 10.8 ANDD37 pKa = 3.99 LTGFDD42 pKa = 5.48 AIFLPGGFSYY52 pKa = 11.39 GDD54 pKa = 3.68 YY55 pKa = 10.83 LRR57 pKa = 11.84 TGAIARR63 pKa = 11.84 FSPIMSAVTQAANDD77 pKa = 3.68 GKK79 pKa = 10.95 LIVGICNGFQILTEE93 pKa = 4.52 AGLLPGQLLVNATPGFICDD112 pKa = 4.02 EE113 pKa = 4.46 VPLSIANAQTAFTNAYY129 pKa = 9.73 DD130 pKa = 3.69 PAEE133 pKa = 4.33 TIMIPIAHH141 pKa = 6.83 GEE143 pKa = 4.09 GNYY146 pKa = 10.25 YY147 pKa = 10.69 ADD149 pKa = 4.09 EE150 pKa = 4.32 ATLALLEE157 pKa = 4.52 EE158 pKa = 4.45 NQQVVFRR165 pKa = 11.84 YY166 pKa = 9.93 VEE168 pKa = 4.1 NPNGSANDD176 pKa = 3.23 IAGIMNEE183 pKa = 3.91 QGNVFGMMPHH193 pKa = 7.33 PEE195 pKa = 4.04 RR196 pKa = 11.84 AVDD199 pKa = 3.78 AVTGNIDD206 pKa = 3.19 GQNFFKK212 pKa = 10.88 SILSGILARR221 pKa = 11.84 AA222 pKa = 3.48
Molecular weight: 23.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 4.05
IPC_protein 4.012
Toseland 3.808
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.923
Rodwell 3.846
Grimsley 3.719
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.935
Sillero 4.139
Patrickios 1.939
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.012
Protein with the highest isoelectric point:
>tr|A0A0Q1DMP6|A0A0Q1DMP6_LEULA DUF3042 family protein OS=Leuconostoc lactis OX=1246 GN=FGL80_04830 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.23 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1614
0
1614
499015
34
1499
309.2
34.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.804 ± 0.072
0.148 ± 0.009
5.929 ± 0.056
5.036 ± 0.066
4.259 ± 0.047
6.77 ± 0.06
2.119 ± 0.027
6.752 ± 0.056
5.185 ± 0.053
9.941 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.025
4.256 ± 0.043
3.831 ± 0.035
5.251 ± 0.067
4.181 ± 0.046
5.131 ± 0.046
6.697 ± 0.055
7.642 ± 0.044
1.08 ± 0.024
3.296 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here