Halococcus saccharolyticus DSM 5350

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halococcaceae; Halococcus; Halococcus saccharolyticus

Average proteome isoelectric point is 4.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0MK97|M0MK97_9EURY Uncharacterized protein OS=Halococcus saccharolyticus DSM 5350 OX=1227455 GN=C449_06081 PE=4 SV=1
MM1 pKa = 7.67AGLAGCTGGGGGGGNGSGGNEE22 pKa = 4.11SGGGANGSSGNGSGSNGSSANGSSSGGSSGNLTVLHH58 pKa = 6.8GWTGGDD64 pKa = 3.55GATALDD70 pKa = 3.74NLVEE74 pKa = 4.47GFEE77 pKa = 4.21EE78 pKa = 4.42ANPDD82 pKa = 3.05VSANFQAIGGGGNTNLDD99 pKa = 3.57TTISNRR105 pKa = 11.84AQNQNLPASWADD117 pKa = 3.34WPGLNLVPFTEE128 pKa = 4.8AGLLADD134 pKa = 4.12VGEE137 pKa = 4.49QVWTDD142 pKa = 3.7DD143 pKa = 3.73LRR145 pKa = 11.84SNYY148 pKa = 8.87SQEE151 pKa = 3.78AKK153 pKa = 9.86TYY155 pKa = 9.15SQVGEE160 pKa = 4.14DD161 pKa = 3.9AGSIGDD167 pKa = 3.87GPYY170 pKa = 9.66VAVPIGSHH178 pKa = 5.89RR179 pKa = 11.84MNDD182 pKa = 2.92LFYY185 pKa = 10.1NVSVVEE191 pKa = 4.13EE192 pKa = 4.39AGVDD196 pKa = 3.52PTSFSQPSDD205 pKa = 3.3LLAAMKK211 pKa = 9.52TVEE214 pKa = 4.42EE215 pKa = 4.17EE216 pKa = 3.72TDD218 pKa = 3.52AVGMAQGLQAPFTTLQLWEE237 pKa = 4.42VVLQGQAGYY246 pKa = 10.32QPFMDD251 pKa = 4.75YY252 pKa = 11.0INGEE256 pKa = 3.88GDD258 pKa = 3.38EE259 pKa = 4.21QAVRR263 pKa = 11.84SAFEE267 pKa = 3.73MVNQYY272 pKa = 10.42FNHH275 pKa = 6.35INQNASSVGFTQANQLIMNGEE296 pKa = 4.31AAFMHH301 pKa = 5.85QGNWVAGAYY310 pKa = 9.77RR311 pKa = 11.84NQDD314 pKa = 2.94GFEE317 pKa = 4.42YY318 pKa = 10.62EE319 pKa = 4.31SDD321 pKa = 3.35WNNVSFPGTDD331 pKa = 3.11DD332 pKa = 3.18MYY334 pKa = 11.66GLHH337 pKa = 7.04LDD339 pKa = 4.25SFPMAASAADD349 pKa = 3.73SQAAQAWLSYY359 pKa = 10.8VGTPDD364 pKa = 2.95AQVRR368 pKa = 11.84FNQYY372 pKa = 9.69KK373 pKa = 10.16GSIPPRR379 pKa = 11.84TDD381 pKa = 2.59VPTDD385 pKa = 3.15EE386 pKa = 4.98FGPYY390 pKa = 8.51LTQTIEE396 pKa = 4.25DD397 pKa = 4.17YY398 pKa = 11.66NNVSNKK404 pKa = 9.65PPTIAHH410 pKa = 6.75GLAVLPDD417 pKa = 3.5VHH419 pKa = 8.25SEE421 pKa = 3.49IDD423 pKa = 3.76GVITDD428 pKa = 4.31SFLGSEE434 pKa = 4.75DD435 pKa = 4.71LDD437 pKa = 4.06AATQGMLDD445 pKa = 3.81AVSGSNN451 pKa = 3.57

Molecular weight: 46.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M0MLV4|M0MLV4_9EURY HTH_11 domain-containing protein OS=Halococcus saccharolyticus DSM 5350 OX=1227455 GN=C449_03336 PE=4 SV=1
MM1 pKa = 7.53SLPTRR6 pKa = 11.84TNSSLVNTPVRR17 pKa = 11.84FGTTIRR23 pKa = 11.84TEE25 pKa = 4.25DD26 pKa = 3.51EE27 pKa = 3.99VGDD30 pKa = 3.78ARR32 pKa = 11.84SVEE35 pKa = 4.18EE36 pKa = 3.56QVGFVGRR43 pKa = 11.84DD44 pKa = 3.19RR45 pKa = 11.84SATSLRR51 pKa = 11.84DD52 pKa = 3.49ATSEE56 pKa = 3.96YY57 pKa = 11.25AKK59 pKa = 10.68NNKK62 pKa = 9.03AALRR66 pKa = 11.84RR67 pKa = 11.84GFRR70 pKa = 11.84KK71 pKa = 10.38DD72 pKa = 3.04GFVATTARR80 pKa = 11.84SGRR83 pKa = 11.84AVWRR87 pKa = 11.84RR88 pKa = 11.84NEE90 pKa = 4.32SVTDD94 pKa = 3.61TNAA97 pKa = 2.89

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3441

0

3441

981310

34

1515

285.2

30.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.244 ± 0.059

0.752 ± 0.012

8.22 ± 0.051

8.205 ± 0.062

3.359 ± 0.027

8.862 ± 0.048

2.068 ± 0.022

4.272 ± 0.031

1.691 ± 0.023

8.79 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.779 ± 0.016

2.472 ± 0.026

4.626 ± 0.026

2.395 ± 0.023

6.559 ± 0.037

5.467 ± 0.035

6.707 ± 0.035

8.764 ± 0.046

1.15 ± 0.015

2.618 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski