Rhizobium sp. PDO1-076

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H4F4A6|H4F4A6_9RHIZ Methylglyoxal synthase OS=Rhizobium sp. PDO1-076 OX=1125979 GN=mgsA PE=3 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAVVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.5FYY49 pKa = 10.61IPGSEE54 pKa = 4.09TCLKK58 pKa = 9.99ISGYY62 pKa = 9.93VRR64 pKa = 11.84FQTEE68 pKa = 3.85FSDD71 pKa = 5.08NGDD74 pKa = 3.64DD75 pKa = 4.54SDD77 pKa = 3.45WNMRR81 pKa = 11.84TRR83 pKa = 11.84AKK85 pKa = 10.96LNFEE89 pKa = 4.53AKK91 pKa = 10.0NDD93 pKa = 4.01SEE95 pKa = 4.78LGTIGSYY102 pKa = 9.9VAVRR106 pKa = 11.84TWADD110 pKa = 3.32NNGSDD115 pKa = 4.29TDD117 pKa = 3.65NKK119 pKa = 11.13LEE121 pKa = 4.05IDD123 pKa = 3.66EE124 pKa = 5.25AFITVGGFKK133 pKa = 10.22VGYY136 pKa = 8.78MYY138 pKa = 10.74NYY140 pKa = 9.05WDD142 pKa = 3.96NDD144 pKa = 3.44LSGEE148 pKa = 4.05TDD150 pKa = 3.98DD151 pKa = 5.36IGSNRR156 pKa = 11.84LNAIGYY162 pKa = 9.54QYY164 pKa = 10.48TADD167 pKa = 3.65AFSAGVFLDD176 pKa = 4.06EE177 pKa = 5.34LSGGYY182 pKa = 9.77SDD184 pKa = 6.14DD185 pKa = 4.34SFDD188 pKa = 3.6VAYY191 pKa = 10.59DD192 pKa = 3.56GSDD195 pKa = 3.25NLGVEE200 pKa = 4.33GQIAATFGVVTAEE213 pKa = 4.21LLGAYY218 pKa = 9.77DD219 pKa = 4.4FAAEE223 pKa = 4.05NGAIRR228 pKa = 11.84AKK230 pKa = 10.64LLADD234 pKa = 3.83VGPGTFGLAAIYY246 pKa = 8.62STGANAYY253 pKa = 9.47YY254 pKa = 10.2DD255 pKa = 3.66VSEE258 pKa = 4.08WTVAAEE264 pKa = 4.16YY265 pKa = 10.44AVKK268 pKa = 10.73LSDD271 pKa = 5.18KK272 pKa = 9.88FTLTPGVQYY281 pKa = 9.73WDD283 pKa = 3.57SYY285 pKa = 11.17GLVSADD291 pKa = 5.43DD292 pKa = 4.13FDD294 pKa = 5.21SSNDD298 pKa = 2.87AWKK301 pKa = 10.74AGLTLDD307 pKa = 3.69YY308 pKa = 10.66KK309 pKa = 10.49IAQGLTAKK317 pKa = 10.44VAVNYY322 pKa = 10.05FDD324 pKa = 5.43EE325 pKa = 5.33DD326 pKa = 3.88AADD329 pKa = 4.07DD330 pKa = 4.46GVWDD334 pKa = 3.96GFVRR338 pKa = 11.84LQRR341 pKa = 11.84SFF343 pKa = 3.42

Molecular weight: 36.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H4F1F9|H4F1F9_9RHIZ Type IV secretion system protein virB4 OS=Rhizobium sp. PDO1-076 OX=1125979 GN=PDO_4199 PE=3 SV=1
MM1 pKa = 7.83TDD3 pKa = 3.13TPTNNANGPASAMPVVVMTAGGLNPTLVIQALAARR38 pKa = 11.84NADD41 pKa = 2.62IHH43 pKa = 7.15VILEE47 pKa = 4.15QPEE50 pKa = 4.35SKK52 pKa = 10.86LDD54 pKa = 3.22ITRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84ARR61 pKa = 11.84RR62 pKa = 11.84LGWVAAIGQLATMIAARR79 pKa = 11.84LLRR82 pKa = 11.84KK83 pKa = 9.19LANKK87 pKa = 10.22RR88 pKa = 11.84IRR90 pKa = 11.84QILDD94 pKa = 2.96AHH96 pKa = 6.32NLTDD100 pKa = 3.63TMPGNVAVHH109 pKa = 5.56QVTSINDD116 pKa = 3.24QEE118 pKa = 4.25TRR120 pKa = 11.84ALIAQIRR127 pKa = 11.84PAAILLVSTRR137 pKa = 11.84LMARR141 pKa = 11.84KK142 pKa = 8.89QLAAMPCPVLNLHH155 pKa = 7.03AGINPAYY162 pKa = 8.78RR163 pKa = 11.84GQMGGYY169 pKa = 8.31WSRR172 pKa = 11.84RR173 pKa = 11.84LNDD176 pKa = 3.14ACNFGATVHH185 pKa = 6.35LVDD188 pKa = 5.34AGTDD192 pKa = 3.37TGGTLYY198 pKa = 10.15QVRR201 pKa = 11.84TTPAAGDD208 pKa = 4.34FISTYY213 pKa = 10.6PLVLTVAALDD223 pKa = 3.53ITCRR227 pKa = 11.84AVADD231 pKa = 4.0ALASNLQPIAPKK243 pKa = 10.55GPSALHH249 pKa = 6.2FPPTLWSWIFHH260 pKa = 6.58GITRR264 pKa = 11.84RR265 pKa = 11.84IWW267 pKa = 3.08

Molecular weight: 28.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5347

0

5347

1610893

30

3141

301.3

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.808 ± 0.044

0.801 ± 0.01

5.842 ± 0.029

5.7 ± 0.029

3.866 ± 0.024

8.258 ± 0.034

2.047 ± 0.016

5.757 ± 0.024

3.646 ± 0.03

10.065 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.017

2.846 ± 0.025

4.767 ± 0.026

3.203 ± 0.023

6.554 ± 0.038

5.902 ± 0.033

5.494 ± 0.035

7.327 ± 0.027

1.237 ± 0.014

2.27 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski