Gloeophyllum trabeum (strain ATCC 11539 / FP-39264 / Madison 617) (Brown rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Gloeophyllales; Gloeophyllaceae; Gloeophyllum; Gloeophyllum trabeum

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S7Q1H5|S7Q1H5_GLOTA Uncharacterized protein OS=Gloeophyllum trabeum (strain ATCC 11539 / FP-39264 / Madison 617) OX=670483 GN=GLOTRDRAFT_131151 PE=4 SV=1
MM1 pKa = 7.22GAYY4 pKa = 9.64YY5 pKa = 10.51DD6 pKa = 4.14EE7 pKa = 5.9IEE9 pKa = 4.69IEE11 pKa = 4.12DD12 pKa = 4.11MAWDD16 pKa = 3.75EE17 pKa = 4.39AKK19 pKa = 10.54GVYY22 pKa = 9.48HH23 pKa = 5.99YY24 pKa = 8.22PCPCGDD30 pKa = 3.21RR31 pKa = 11.84FEE33 pKa = 5.22ISRR36 pKa = 11.84QQLRR40 pKa = 11.84DD41 pKa = 3.44YY42 pKa = 11.03EE43 pKa = 5.37DD44 pKa = 3.51IATCPSCSLIIRR56 pKa = 11.84VIYY59 pKa = 10.38DD60 pKa = 3.49PLDD63 pKa = 3.86FQDD66 pKa = 6.21DD67 pKa = 3.8DD68 pKa = 6.63DD69 pKa = 4.97EE70 pKa = 5.1DD71 pKa = 3.72QGSGEE76 pKa = 4.38GEE78 pKa = 4.03VKK80 pKa = 10.38EE81 pKa = 4.33SEE83 pKa = 4.34EE84 pKa = 4.32SGSEE88 pKa = 3.96SGDD91 pKa = 3.47EE92 pKa = 4.01QFEE95 pKa = 4.27DD96 pKa = 3.82ALEE99 pKa = 4.09KK100 pKa = 11.27LEE102 pKa = 4.18ISEE105 pKa = 4.46GPQVIAAAAA114 pKa = 3.55

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S7QDD6|S7QDD6_GLOTA ATP synthase subunit 5 mitochondrial OS=Gloeophyllum trabeum (strain ATCC 11539 / FP-39264 / Madison 617) OX=670483 GN=GLOTRDRAFT_137737 PE=3 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84IFRR6 pKa = 11.84QVALALARR14 pKa = 11.84PPTLSQASSTVSSLLQSQRR33 pKa = 11.84LSTPRR38 pKa = 11.84PTVLAPSIAFQSHH51 pKa = 5.94SLLAPTFTGLPSALLRR67 pKa = 11.84LNQVRR72 pKa = 11.84WGSRR76 pKa = 11.84GTEE79 pKa = 3.93YY80 pKa = 10.77QPSQRR85 pKa = 11.84VRR87 pKa = 11.84KK88 pKa = 9.28RR89 pKa = 11.84RR90 pKa = 11.84HH91 pKa = 4.87GFLARR96 pKa = 11.84KK97 pKa = 9.53KK98 pKa = 9.03SQHH101 pKa = 5.36GPKK104 pKa = 9.63ILARR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84AKK112 pKa = 9.85GRR114 pKa = 11.84KK115 pKa = 8.43FLSHH119 pKa = 6.98

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11735

0

11735

4956750

49

3936

422.4

46.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.96 ± 0.022

1.245 ± 0.009

5.615 ± 0.016

6.187 ± 0.022

3.554 ± 0.013

6.784 ± 0.019

2.468 ± 0.011

4.553 ± 0.016

4.513 ± 0.02

9.171 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.009

3.164 ± 0.012

6.602 ± 0.03

3.673 ± 0.014

6.506 ± 0.019

8.343 ± 0.032

5.766 ± 0.015

6.54 ± 0.016

1.499 ± 0.009

2.769 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski