Pseudohongiella acticola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Pseudohongiella

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E8CIN9|A0A1E8CIN9_9GAMM Uncharacterized protein OS=Pseudohongiella acticola OX=1524254 GN=PHACT_03510 PE=4 SV=1
MM1 pKa = 6.47TTAINPPAVLGEE13 pKa = 4.7DD14 pKa = 3.36EE15 pKa = 4.48DD16 pKa = 5.4ANYY19 pKa = 10.56QLFLEE24 pKa = 4.81QVQHH28 pKa = 6.63TGQVWGLHH36 pKa = 6.4GEE38 pKa = 4.53DD39 pKa = 4.13GWALCPSVEE48 pKa = 4.2FEE50 pKa = 4.13EE51 pKa = 5.11TEE53 pKa = 4.33VFPFWSDD60 pKa = 2.76EE61 pKa = 4.08RR62 pKa = 11.84YY63 pKa = 9.76AAALCTDD70 pKa = 3.45EE71 pKa = 3.98WSTYY75 pKa = 8.9KK76 pKa = 10.56PKK78 pKa = 10.61AISLQDD84 pKa = 4.0FATEE88 pKa = 4.12WLPGIHH94 pKa = 7.13EE95 pKa = 5.05DD96 pKa = 3.98NAMVGPNWDD105 pKa = 3.56TEE107 pKa = 4.23MSGLEE112 pKa = 4.38LEE114 pKa = 4.66PADD117 pKa = 3.64MAAALSADD125 pKa = 3.33

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E8CLI0|A0A1E8CLI0_9GAMM Uncharacterized protein OS=Pseudohongiella acticola OX=1524254 GN=PHACT_08180 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLLKK11 pKa = 10.3RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84LVIKK32 pKa = 10.33RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.75LSAA44 pKa = 4.0

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3105

0

3105

1076679

44

4238

346.8

38.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.302 ± 0.051

0.918 ± 0.013

6.124 ± 0.033

5.7 ± 0.04

3.764 ± 0.025

7.591 ± 0.049

2.122 ± 0.021

5.424 ± 0.035

2.963 ± 0.036

10.63 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.022

3.57 ± 0.031

4.492 ± 0.027

4.551 ± 0.034

6.145 ± 0.038

6.422 ± 0.03

5.439 ± 0.037

7.215 ± 0.036

1.332 ± 0.015

2.677 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski