Lake Sarah-associated circular virus-5

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA52|A0A126GA52_9VIRU Coat protein OS=Lake Sarah-associated circular virus-5 OX=1685779 PE=4 SV=1
MM1 pKa = 7.29STRR4 pKa = 11.84QRR6 pKa = 11.84HH7 pKa = 4.87RR8 pKa = 11.84SVCFTLNNYY17 pKa = 7.39TAQEE21 pKa = 4.05KK22 pKa = 7.89QHH24 pKa = 6.47IKK26 pKa = 10.74DD27 pKa = 3.47GADD30 pKa = 2.94AGSFKK35 pKa = 11.11YY36 pKa = 10.28IVFQEE41 pKa = 3.73EE42 pKa = 4.02RR43 pKa = 11.84GAVGTPHH50 pKa = 6.32LQGYY54 pKa = 7.26AQRR57 pKa = 11.84AQPTEE62 pKa = 3.77FKK64 pKa = 8.69TWKK67 pKa = 9.98RR68 pKa = 11.84FLGDD72 pKa = 3.26RR73 pKa = 11.84CHH75 pKa = 8.13LEE77 pKa = 4.13GSKK80 pKa = 9.29GTPARR85 pKa = 11.84NRR87 pKa = 11.84EE88 pKa = 4.26YY89 pKa = 9.96CTKK92 pKa = 10.6DD93 pKa = 2.87ADD95 pKa = 4.96RR96 pKa = 11.84IPGTLYY102 pKa = 10.39FEE104 pKa = 4.68KK105 pKa = 11.11GEE107 pKa = 4.18VPVQGEE113 pKa = 4.46RR114 pKa = 11.84NDD116 pKa = 3.34LTAIFEE122 pKa = 4.14AAKK125 pKa = 10.51DD126 pKa = 3.66STVSVRR132 pKa = 11.84DD133 pKa = 3.6LVDD136 pKa = 3.21TNGAAFIRR144 pKa = 11.84YY145 pKa = 8.26YY146 pKa = 10.81KK147 pKa = 10.63GVLAVRR153 pKa = 11.84SIFSDD158 pKa = 2.72KK159 pKa = 10.41RR160 pKa = 11.84QRR162 pKa = 11.84KK163 pKa = 6.48TKK165 pKa = 10.1VFWFYY170 pKa = 11.63GPTGLGKK177 pKa = 9.74SHH179 pKa = 6.65EE180 pKa = 4.77CNRR183 pKa = 11.84LAPNAFWKK191 pKa = 10.49QNSPWWCGYY200 pKa = 9.78DD201 pKa = 4.03ANEE204 pKa = 4.86HH205 pKa = 6.69DD206 pKa = 6.14DD207 pKa = 4.64IVIDD211 pKa = 4.51DD212 pKa = 4.04YY213 pKa = 11.73RR214 pKa = 11.84ADD216 pKa = 3.52FCKK219 pKa = 10.22FAQLLRR225 pKa = 11.84LFDD228 pKa = 3.85FTKK231 pKa = 10.08LTVEE235 pKa = 4.35TKK237 pKa = 10.39GGNVNFRR244 pKa = 11.84AKK246 pKa = 10.25RR247 pKa = 11.84IFISTPRR254 pKa = 11.84SPLEE258 pKa = 3.61TWIGRR263 pKa = 11.84SEE265 pKa = 4.2EE266 pKa = 5.22DD267 pKa = 2.77IQQLLRR273 pKa = 11.84RR274 pKa = 11.84IEE276 pKa = 4.04VLVEE280 pKa = 4.15FTPSPFTDD288 pKa = 3.19NGRR291 pKa = 11.84VLPNKK296 pKa = 9.23VFHH299 pKa = 6.81KK300 pKa = 10.6GSPDD304 pKa = 3.61DD305 pKa = 5.28LVVDD309 pKa = 4.54DD310 pKa = 6.13LGDD313 pKa = 4.02GDD315 pKa = 3.98QQPRR319 pKa = 11.84SPVEE323 pKa = 4.03TPPEE327 pKa = 3.93EE328 pKa = 4.24AVEE331 pKa = 4.25DD332 pKa = 3.97ASDD335 pKa = 3.13RR336 pKa = 11.84RR337 pKa = 11.84TRR339 pKa = 11.84PRR341 pKa = 11.84VEE343 pKa = 4.01EE344 pKa = 3.79ADD346 pKa = 3.67EE347 pKa = 4.2PRR349 pKa = 11.84EE350 pKa = 3.78QFAQDD355 pKa = 3.02EE356 pKa = 4.72YY357 pKa = 11.83GFLNEE362 pKa = 4.76FDD364 pKa = 4.71EE365 pKa = 6.17LNDD368 pKa = 4.85DD369 pKa = 5.03DD370 pKa = 4.8FTFF373 pKa = 4.39

Molecular weight: 42.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA52|A0A126GA52_9VIRU Coat protein OS=Lake Sarah-associated circular virus-5 OX=1685779 PE=4 SV=1
MM1 pKa = 7.49MSMSMYY7 pKa = 10.25SGSGGGKK14 pKa = 9.03SSYY17 pKa = 10.37SINYY21 pKa = 7.48SSKK24 pKa = 10.19RR25 pKa = 11.84GRR27 pKa = 11.84SPTPYY32 pKa = 10.51SSFKK36 pKa = 9.9SARR39 pKa = 11.84VQPVNQTTASAINMEE54 pKa = 4.42HH55 pKa = 7.2LNRR58 pKa = 11.84TEE60 pKa = 3.81IYY62 pKa = 10.29LGRR65 pKa = 11.84TVATASGSKK74 pKa = 10.39AYY76 pKa = 10.26GGSKK80 pKa = 9.93ANKK83 pKa = 9.29GKK85 pKa = 10.53GSKK88 pKa = 9.48ISYY91 pKa = 8.67DD92 pKa = 3.2AYY94 pKa = 10.53MNTLFPPYY102 pKa = 8.79SQTVIGYY109 pKa = 9.5GNNYY113 pKa = 8.9YY114 pKa = 11.03NKK116 pKa = 9.24TKK118 pKa = 10.66NATNSASAGDD128 pKa = 4.15DD129 pKa = 3.05KK130 pKa = 11.4SIYY133 pKa = 9.23IAQGRR138 pKa = 11.84QAWRR142 pKa = 11.84EE143 pKa = 3.86YY144 pKa = 10.66VAMPFQRR151 pKa = 11.84TAGTNGWGDD160 pKa = 4.2LFTTSVTDD168 pKa = 4.3LLVKK172 pKa = 10.6AQDD175 pKa = 3.67VLTTAVAQSVYY186 pKa = 8.15TTTTLVPSSTTTVAEE201 pKa = 4.07RR202 pKa = 11.84QKK204 pKa = 10.74LQNLSFHH211 pKa = 6.52YY212 pKa = 10.06SGGYY216 pKa = 7.73QRR218 pKa = 11.84HH219 pKa = 5.7KK220 pKa = 10.63FVNTGNSKK228 pKa = 8.44ITIEE232 pKa = 4.04FFEE235 pKa = 4.48AKK237 pKa = 9.34PRR239 pKa = 11.84NPMPAYY245 pKa = 9.93GSSATYY251 pKa = 8.78TVNSIGASIVQDD263 pKa = 3.3MQTNVPRR270 pKa = 11.84DD271 pKa = 3.39NSFYY275 pKa = 10.4PSYY278 pKa = 10.57DD279 pKa = 3.06TTDD282 pKa = 3.55FDD284 pKa = 5.4NINDD288 pKa = 3.49QLVRR292 pKa = 11.84LNAQCATTNYY302 pKa = 9.97KK303 pKa = 8.86WLCNKK308 pKa = 8.91PVKK311 pKa = 10.4HH312 pKa = 5.8VVDD315 pKa = 4.03PGGEE319 pKa = 4.3FIYY322 pKa = 10.64TMGIDD327 pKa = 2.96AFEE330 pKa = 4.39FKK332 pKa = 10.58EE333 pKa = 4.63SEE335 pKa = 4.29FNTLPGLTSTDD346 pKa = 2.71ISGVQWFYY354 pKa = 11.31PKK356 pKa = 9.06FTKK359 pKa = 10.48FLVCRR364 pKa = 11.84FTTEE368 pKa = 3.95IGHH371 pKa = 5.58STDD374 pKa = 3.2GVSGFDD380 pKa = 3.12QTYY383 pKa = 8.16TGVGYY388 pKa = 10.66VEE390 pKa = 4.61GMMAHH395 pKa = 6.72TCTEE399 pKa = 3.83HH400 pKa = 6.09HH401 pKa = 6.81NCRR404 pKa = 11.84FMPIIYY410 pKa = 8.37THH412 pKa = 6.81SNVTNNRR419 pKa = 11.84LDD421 pKa = 3.69KK422 pKa = 10.18DD423 pKa = 3.54TAAIKK428 pKa = 10.7VVFNDD433 pKa = 3.3ATGAPTNVMDD443 pKa = 5.81DD444 pKa = 3.47VV445 pKa = 3.93

Molecular weight: 49.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

818

373

445

409.0

45.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.724 ± 0.181

1.345 ± 0.165

6.724 ± 1.33

5.134 ± 1.653

5.746 ± 0.767

7.335 ± 0.396

1.956 ± 0.05

3.912 ± 0.267

5.501 ± 0.081

4.89 ± 0.799

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.98

5.623 ± 1.003

4.645 ± 0.449

4.156 ± 0.251

5.868 ± 1.698

7.213 ± 1.662

9.046 ± 1.636

6.479 ± 0.196

1.222 ± 0.242

4.645 ± 1.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski