Rhodococcus opacus PD630

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; Rhodococcus opacus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G9MCY7|A0A3G9MCY7_RHOOP Cytochrome bc1 complex cytochrome b subunit OS=Rhodococcus opacus PD630 OX=543736 GN=OPAG_05880 PE=4 SV=1
MM1 pKa = 7.15TLVPRR6 pKa = 11.84AAVTLIAAGPLAVFLAAPAAADD28 pKa = 3.94PPDD31 pKa = 3.72VTLTAAVAGSTVTTTITNDD50 pKa = 2.79TGADD54 pKa = 3.47IVCGLGGLHH63 pKa = 6.88AADD66 pKa = 5.66DD67 pKa = 4.08IVNPEE72 pKa = 3.96SVAVFHH78 pKa = 6.71EE79 pKa = 4.88SNVVIGPGPRR89 pKa = 11.84DD90 pKa = 3.46FVFDD94 pKa = 4.16AVPDD98 pKa = 3.96GDD100 pKa = 5.22YY101 pKa = 10.66LIHH104 pKa = 6.96WICSEE109 pKa = 3.85NAGPGDD115 pKa = 4.46EE116 pKa = 4.5VWGSAPLPEE125 pKa = 3.82TAYY128 pKa = 10.52AKK130 pKa = 10.06PATADD135 pKa = 3.79PLPVTVKK142 pKa = 10.18TEE144 pKa = 3.87TCFGSVCLPPGLGLL158 pKa = 4.12

Molecular weight: 15.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G9M5G9|A0A3G9M5G9_RHOOP ATP synthase subunit C (Fragment) OS=Rhodococcus opacus PD630 OX=543736 GN=OPAG_08530 PE=3 SV=1
PP1 pKa = 7.33PPARR5 pKa = 11.84PDD7 pKa = 3.13EE8 pKa = 4.55RR9 pKa = 11.84GRR11 pKa = 11.84RR12 pKa = 11.84TPRR15 pKa = 11.84PAGRR19 pKa = 11.84RR20 pKa = 11.84LPPHH24 pKa = 6.18APGRR28 pKa = 11.84RR29 pKa = 11.84PRR31 pKa = 11.84RR32 pKa = 11.84PDD34 pKa = 2.48GRR36 pKa = 11.84RR37 pKa = 11.84PRR39 pKa = 11.84RR40 pKa = 11.84TPGQGRR46 pKa = 11.84RR47 pKa = 11.84HH48 pKa = 5.42RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84LRR54 pKa = 11.84PQRR57 pKa = 11.84HH58 pKa = 4.7RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84LRR63 pKa = 11.84HH64 pKa = 5.34AGRR67 pKa = 11.84RR68 pKa = 11.84HH69 pKa = 5.89RR70 pKa = 11.84PRR72 pKa = 11.84PVHH75 pKa = 6.48RR76 pKa = 11.84PPPRR80 pKa = 11.84DD81 pKa = 3.22RR82 pKa = 11.84RR83 pKa = 11.84PVPRR87 pKa = 11.84PGQDD91 pKa = 2.44HH92 pKa = 7.1HH93 pKa = 6.91LQQPRR98 pKa = 11.84TRR100 pKa = 11.84TT101 pKa = 3.36

Molecular weight: 12.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3247

0

3247

964148

23

8928

296.9

31.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.583 ± 0.054

0.835 ± 0.015

6.215 ± 0.041

5.461 ± 0.039

3.086 ± 0.023

8.789 ± 0.044

2.334 ± 0.022

4.24 ± 0.034

2.104 ± 0.036

9.918 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.02

2.124 ± 0.024

5.638 ± 0.032

2.873 ± 0.024

7.495 ± 0.05

5.773 ± 0.029

6.392 ± 0.036

8.665 ± 0.046

1.451 ± 0.017

2.058 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski