Amino acid dipepetide frequency for Rhodococcus opacus PD630

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.565AlaAla: 18.565 ± 0.216	1.003AlaCys: 1.003 ± 0.032	7.886AlaAsp: 7.886 ± 0.095	7.675AlaGlu: 7.675 ± 0.111	3.569AlaPhe: 3.569 ± 0.064	11.269AlaGly: 11.269 ± 0.125	2.781AlaHis: 2.781 ± 0.049	4.856AlaIle: 4.856 ± 0.085	2.688AlaLys: 2.688 ± 0.07	13.342AlaLeu: 13.342 ± 0.147	2.62AlaMet: 2.62 ± 0.053	2.272AlaAsn: 2.272 ± 0.055	6.12AlaPro: 6.12 ± 0.096	3.706AlaGln: 3.706 ± 0.056	8.574AlaArg: 8.574 ± 0.118	6.254AlaSer: 6.254 ± 0.087	7.387AlaThr: 7.387 ± 0.093	11.425AlaVal: 11.425 ± 0.122	1.558AlaTrp: 1.558 ± 0.043	2.283AlaTyr: 2.283 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.053CysAla: 1.053 ± 0.036	0.109CysCys: 0.109 ± 0.011	0.461CysAsp: 0.461 ± 0.022	0.447CysGlu: 0.447 ± 0.022	0.216CysPhe: 0.216 ± 0.015	0.986CysGly: 0.986 ± 0.041	0.204CysHis: 0.204 ± 0.015	0.297CysIle: 0.297 ± 0.019	0.12CysLys: 0.12 ± 0.011	0.685CysLeu: 0.685 ± 0.024	0.13CysMet: 0.13 ± 0.012	0.172CysAsn: 0.172 ± 0.014	0.53CysPro: 0.53 ± 0.025	0.19CysGln: 0.19 ± 0.015	0.675CysArg: 0.675 ± 0.031	0.585CysSer: 0.585 ± 0.024	0.54CysThr: 0.54 ± 0.023	0.659CysVal: 0.659 ± 0.024	0.124CysTrp: 0.124 ± 0.013	0.17CysTyr: 0.17 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.737AspAla: 7.737 ± 0.096	0.438AspCys: 0.438 ± 0.022	3.897AspAsp: 3.897 ± 0.08	3.971AspGlu: 3.971 ± 0.075	1.738AspPhe: 1.738 ± 0.04	5.567AspGly: 5.567 ± 0.083	1.519AspHis: 1.519 ± 0.039	2.418AspIle: 2.418 ± 0.06	1.2AspLys: 1.2 ± 0.046	6.543AspLeu: 6.543 ± 0.106	0.891AspMet: 0.891 ± 0.03	1.152AspAsn: 1.152 ± 0.036	4.285AspPro: 4.285 ± 0.073	1.662AspGln: 1.662 ± 0.046	5.084AspArg: 5.084 ± 0.091	3.076AspSer: 3.076 ± 0.066	3.496AspThr: 3.496 ± 0.075	5.248AspVal: 5.248 ± 0.098	0.925AspTrp: 0.925 ± 0.036	1.302AspTyr: 1.302 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	5.861GluAla: 5.861 ± 0.088	0.424GluCys: 0.424 ± 0.02	2.737GluAsp: 2.737 ± 0.055	2.873GluGlu: 2.873 ± 0.07	1.829GluPhe: 1.829 ± 0.05	3.55GluGly: 3.55 ± 0.072	1.628GluHis: 1.628 ± 0.041	2.717GluIle: 2.717 ± 0.056	1.509GluLys: 1.509 ± 0.044	6.473GluLeu: 6.473 ± 0.122	1.042GluMet: 1.042 ± 0.033	1.388GluAsn: 1.388 ± 0.041	2.967GluPro: 2.967 ± 0.064	2.377GluGln: 2.377 ± 0.057	4.632GluArg: 4.632 ± 0.078	3.244GluSer: 3.244 ± 0.057	2.962GluThr: 2.962 ± 0.06	4.349GluVal: 4.349 ± 0.069	0.826GluTrp: 0.826 ± 0.032	1.22GluTyr: 1.22 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.116PheAla: 4.116 ± 0.075	0.325PheCys: 0.325 ± 0.019	2.612PheAsp: 2.612 ± 0.066	1.64PheGlu: 1.64 ± 0.042	1.062PhePhe: 1.062 ± 0.044	3.463PheGly: 3.463 ± 0.072	0.674PheHis: 0.674 ± 0.026	1.048PheIle: 1.048 ± 0.038	0.504PheLys: 0.504 ± 0.023	2.677PheLeu: 2.677 ± 0.057	0.482PheMet: 0.482 ± 0.021	0.588PheAsn: 0.588 ± 0.028	1.455PhePro: 1.455 ± 0.04	0.715PheGln: 0.715 ± 0.029	1.766PheArg: 1.766 ± 0.049	1.726PheSer: 1.726 ± 0.044	2.166PheThr: 2.166 ± 0.062	2.714PheVal: 2.714 ± 0.06	0.443PheTrp: 0.443 ± 0.025	0.69PheTyr: 0.69 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.942GlyAla: 9.942 ± 0.125	0.814GlyCys: 0.814 ± 0.028	4.957GlyAsp: 4.957 ± 0.079	4.461GlyGlu: 4.461 ± 0.073	3.169GlyPhe: 3.169 ± 0.056	7.696GlyGly: 7.696 ± 0.115	2.068GlyHis: 2.068 ± 0.05	4.4GlyIle: 4.4 ± 0.071	2.168GlyLys: 2.168 ± 0.055	8.398GlyLeu: 8.398 ± 0.115	2.007GlyMet: 2.007 ± 0.048	1.888GlyAsn: 1.888 ± 0.044	4.347GlyPro: 4.347 ± 0.071	2.516GlyGln: 2.516 ± 0.062	6.597GlyArg: 6.597 ± 0.083	5.38GlySer: 5.38 ± 0.078	5.717GlyThr: 5.717 ± 0.089	7.503GlyVal: 7.503 ± 0.09	1.534GlyTrp: 1.534 ± 0.039	2.328GlyTyr: 2.328 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.57HisAla: 2.57 ± 0.055	0.245HisCys: 0.245 ± 0.017	1.397HisAsp: 1.397 ± 0.037	1.2HisGlu: 1.2 ± 0.041	0.676HisPhe: 0.676 ± 0.027	2.153HisGly: 2.153 ± 0.055	0.836HisHis: 0.836 ± 0.038	0.845HisIle: 0.845 ± 0.03	0.353HisLys: 0.353 ± 0.021	2.313HisLeu: 2.313 ± 0.047	0.444HisMet: 0.444 ± 0.021	0.492HisAsn: 0.492 ± 0.021	1.746HisPro: 1.746 ± 0.05	0.675HisGln: 0.675 ± 0.026	2.185HisArg: 2.185 ± 0.057	1.251HisSer: 1.251 ± 0.034	1.322HisThr: 1.322 ± 0.04	1.734HisVal: 1.734 ± 0.042	0.361HisTrp: 0.361 ± 0.019	0.547HisTyr: 0.547 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.295IleAla: 6.295 ± 0.081	0.446IleCys: 0.446 ± 0.022	3.295IleAsp: 3.295 ± 0.063	2.589IleGlu: 2.589 ± 0.058	1.092IlePhe: 1.092 ± 0.037	4.583IleGly: 4.583 ± 0.078	0.779IleHis: 0.779 ± 0.031	1.358IleIle: 1.358 ± 0.043	0.758IleLys: 0.758 ± 0.035	3.538IleLeu: 3.538 ± 0.065	0.641IleMet: 0.641 ± 0.029	0.917IleAsn: 0.917 ± 0.032	2.411IlePro: 2.411 ± 0.045	0.875IleGln: 0.875 ± 0.033	2.745IleArg: 2.745 ± 0.056	2.378IleSer: 2.378 ± 0.054	2.737IleThr: 2.737 ± 0.06	3.815IleVal: 3.815 ± 0.073	0.385IleTrp: 0.385 ± 0.021	0.763IleTyr: 0.763 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.611LysAla: 2.611 ± 0.07	0.12LysCys: 0.12 ± 0.01	1.094LysAsp: 1.094 ± 0.04	1.032LysGlu: 1.032 ± 0.041	0.621LysPhe: 0.621 ± 0.026	1.527LysGly: 1.527 ± 0.041	0.442LysHis: 0.442 ± 0.021	1.115LysIle: 1.115 ± 0.037	0.797LysLys: 0.797 ± 0.046	2.072LysLeu: 2.072 ± 0.046	0.466LysMet: 0.466 ± 0.025	0.577LysAsn: 0.577 ± 0.028	1.206LysPro: 1.206 ± 0.04	0.703LysGln: 0.703 ± 0.029	1.502LysArg: 1.502 ± 0.047	1.276LysSer: 1.276 ± 0.045	1.357LysThr: 1.357 ± 0.042	1.751LysVal: 1.751 ± 0.046	0.291LysTrp: 0.291 ± 0.016	0.481LysTyr: 0.481 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.459LeuAla: 13.459 ± 0.137	0.833LeuCys: 0.833 ± 0.03	6.912LeuAsp: 6.912 ± 0.123	4.834LeuGlu: 4.834 ± 0.078	2.873LeuPhe: 2.873 ± 0.059	8.908LeuGly: 8.908 ± 0.108	2.187LeuHis: 2.187 ± 0.056	4.062LeuIle: 4.062 ± 0.08	1.685LeuLys: 1.685 ± 0.048	9.741LeuLeu: 9.741 ± 0.133	1.779LeuMet: 1.779 ± 0.051	1.872LeuAsn: 1.872 ± 0.044	5.674LeuPro: 5.674 ± 0.08	2.478LeuGln: 2.478 ± 0.052	7.534LeuArg: 7.534 ± 0.098	5.851LeuSer: 5.851 ± 0.093	6.765LeuThr: 6.765 ± 0.088	8.781LeuVal: 8.781 ± 0.118	1.262LeuTrp: 1.262 ± 0.038	1.691LeuTyr: 1.691 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.263MetAla: 2.263 ± 0.052	0.189MetCys: 0.189 ± 0.014	0.885MetAsp: 0.885 ± 0.035	0.699MetGlu: 0.699 ± 0.026	0.622MetPhe: 0.622 ± 0.026	1.475MetGly: 1.475 ± 0.047	0.428MetHis: 0.428 ± 0.022	0.971MetIle: 0.971 ± 0.034	0.52MetLys: 0.52 ± 0.024	1.977MetLeu: 1.977 ± 0.05	0.403MetMet: 0.403 ± 0.021	0.565MetAsn: 0.565 ± 0.025	1.096MetPro: 1.096 ± 0.032	0.559MetGln: 0.559 ± 0.024	1.433MetArg: 1.433 ± 0.036	1.608MetSer: 1.608 ± 0.046	1.802MetThr: 1.802 ± 0.045	1.568MetVal: 1.568 ± 0.043	0.236MetTrp: 0.236 ± 0.017	0.353MetTyr: 0.353 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.508AsnAla: 2.508 ± 0.054	0.172AsnCys: 0.172 ± 0.015	1.069AsnAsp: 1.069 ± 0.031	1.003AsnGlu: 1.003 ± 0.034	0.635AsnPhe: 0.635 ± 0.024	1.874AsnGly: 1.874 ± 0.049	0.447AsnHis: 0.447 ± 0.021	0.914AsnIle: 0.914 ± 0.032	0.48AsnLys: 0.48 ± 0.023	2.032AsnLeu: 2.032 ± 0.049	0.418AsnMet: 0.418 ± 0.022	0.536AsnAsn: 0.536 ± 0.026	1.682AsnPro: 1.682 ± 0.036	0.638AsnGln: 0.638 ± 0.029	1.581AsnArg: 1.581 ± 0.038	1.232AsnSer: 1.232 ± 0.035	1.433AsnThr: 1.433 ± 0.038	1.709AsnVal: 1.709 ± 0.048	0.34AsnTrp: 0.34 ± 0.02	0.534AsnTyr: 0.534 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.102ProAla: 7.102 ± 0.12	0.301ProCys: 0.301 ± 0.019	4.31ProAsp: 4.31 ± 0.071	3.824ProGlu: 3.824 ± 0.066	1.574ProPhe: 1.574 ± 0.043	5.434ProGly: 5.434 ± 0.088	1.344ProHis: 1.344 ± 0.04	2.213ProIle: 2.213 ± 0.044	1.142ProLys: 1.142 ± 0.035	4.735ProLeu: 4.735 ± 0.083	1.149ProMet: 1.149 ± 0.029	1.178ProAsn: 1.178 ± 0.038	2.993ProPro: 2.993 ± 0.084	1.625ProGln: 1.625 ± 0.046	3.689ProArg: 3.689 ± 0.069	3.228ProSer: 3.228 ± 0.054	3.691ProThr: 3.691 ± 0.071	4.999ProVal: 4.999 ± 0.087	0.791ProTrp: 0.791 ± 0.03	1.051ProTyr: 1.051 ± 0.037	0.001ProXaa: 0.001 ± 0.001
Gln	3.341GlnAla: 3.341 ± 0.065	0.193GlnCys: 0.193 ± 0.014	1.267GlnAsp: 1.267 ± 0.036	1.211GlnGlu: 1.211 ± 0.044	0.961GlnPhe: 0.961 ± 0.031	1.984GlnGly: 1.984 ± 0.053	0.747GlnHis: 0.747 ± 0.031	1.481GlnIle: 1.481 ± 0.042	0.663GlnLys: 0.663 ± 0.033	3.343GlnLeu: 3.343 ± 0.057	0.593GlnMet: 0.593 ± 0.026	0.606GlnAsn: 0.606 ± 0.024	1.615GlnPro: 1.615 ± 0.054	1.315GlnGln: 1.315 ± 0.047	2.521GlnArg: 2.521 ± 0.052	1.62GlnSer: 1.62 ± 0.043	1.507GlnThr: 1.507 ± 0.04	2.544GlnVal: 2.544 ± 0.059	0.595GlnTrp: 0.595 ± 0.026	0.62GlnTyr: 0.62 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	8.148ArgAla: 8.148 ± 0.101	0.66ArgCys: 0.66 ± 0.027	4.259ArgAsp: 4.259 ± 0.068	4.167ArgGlu: 4.167 ± 0.066	2.443ArgPhe: 2.443 ± 0.058	5.433ArgGly: 5.433 ± 0.072	1.964ArgHis: 1.964 ± 0.058	3.664ArgIle: 3.664 ± 0.063	1.749ArgLys: 1.749 ± 0.047	7.299ArgLeu: 7.299 ± 0.095	1.749ArgMet: 1.749 ± 0.042	1.774ArgAsn: 1.774 ± 0.046	4.182ArgPro: 4.182 ± 0.081	2.272ArgGln: 2.272 ± 0.05	7.136ArgArg: 7.136 ± 0.139	4.555ArgSer: 4.555 ± 0.078	4.974ArgThr: 4.974 ± 0.086	5.534ArgVal: 5.534 ± 0.074	1.257ArgTrp: 1.257 ± 0.037	1.73ArgTyr: 1.73 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	7.193SerAla: 7.193 ± 0.097	0.492SerCys: 0.492 ± 0.022	3.477SerAsp: 3.477 ± 0.059	3.034SerGlu: 3.034 ± 0.06	1.819SerPhe: 1.819 ± 0.046	5.801SerGly: 5.801 ± 0.079	1.143SerHis: 1.143 ± 0.034	2.294SerIle: 2.294 ± 0.05	1.198SerLys: 1.198 ± 0.039	5.148SerLeu: 5.148 ± 0.075	1.399SerMet: 1.399 ± 0.032	1.262SerAsn: 1.262 ± 0.035	3.338SerPro: 3.338 ± 0.063	1.484SerGln: 1.484 ± 0.037	4.319SerArg: 4.319 ± 0.074	3.575SerSer: 3.575 ± 0.061	3.808SerThr: 3.808 ± 0.065	4.841SerVal: 4.841 ± 0.07	0.891SerTrp: 0.891 ± 0.03	1.218SerTyr: 1.218 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	8.108ThrAla: 8.108 ± 0.098	0.466ThrCys: 0.466 ± 0.024	3.914ThrAsp: 3.914 ± 0.085	3.516ThrGlu: 3.516 ± 0.069	1.964ThrPhe: 1.964 ± 0.054	6.141ThrGly: 6.141 ± 0.081	1.382ThrHis: 1.382 ± 0.045	2.61ThrIle: 2.61 ± 0.053	1.232ThrLys: 1.232 ± 0.042	5.924ThrLeu: 5.924 ± 0.081	1.17ThrMet: 1.17 ± 0.032	1.243ThrAsn: 1.243 ± 0.036	4.094ThrPro: 4.094 ± 0.076	1.574ThrGln: 1.574 ± 0.045	4.066ThrArg: 4.066 ± 0.059	3.765ThrSer: 3.765 ± 0.067	4.37ThrThr: 4.37 ± 0.089	6.224ThrVal: 6.224 ± 0.085	0.878ThrTrp: 0.878 ± 0.033	1.281ThrTyr: 1.281 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	11.145ValAla: 11.145 ± 0.13	0.742ValCys: 0.742 ± 0.024	5.772ValAsp: 5.772 ± 0.088	4.729ValGlu: 4.729 ± 0.082	2.694ValPhe: 2.694 ± 0.062	6.961ValGly: 6.961 ± 0.102	1.98ValHis: 1.98 ± 0.052	3.766ValIle: 3.766 ± 0.064	1.561ValLys: 1.561 ± 0.049	9.089ValLeu: 9.089 ± 0.124	1.603ValMet: 1.603 ± 0.043	1.881ValAsn: 1.881 ± 0.049	4.761ValPro: 4.761 ± 0.088	2.128ValGln: 2.128 ± 0.047	5.98ValArg: 5.98 ± 0.093	4.868ValSer: 4.868 ± 0.069	5.765ValThr: 5.765 ± 0.096	8.558ValVal: 8.558 ± 0.121	1.12ValTrp: 1.12 ± 0.034	1.551ValTyr: 1.551 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.526TrpAla: 1.526 ± 0.04	0.169TrpCys: 0.169 ± 0.013	0.726TrpAsp: 0.726 ± 0.032	0.635TrpGlu: 0.635 ± 0.03	0.556TrpPhe: 0.556 ± 0.024	1.08TrpGly: 1.08 ± 0.033	0.351TrpHis: 0.351 ± 0.02	0.704TrpIle: 0.704 ± 0.023	0.354TrpLys: 0.354 ± 0.019	1.534TrpLeu: 1.534 ± 0.044	0.333TrpMet: 0.333 ± 0.02	0.43TrpAsn: 0.43 ± 0.022	0.691TrpPro: 0.691 ± 0.03	0.543TrpGln: 0.543 ± 0.023	1.171TrpArg: 1.171 ± 0.037	0.987TrpSer: 0.987 ± 0.03	0.949TrpThr: 0.949 ± 0.034	1.129TrpVal: 1.129 ± 0.038	0.32TrpTrp: 0.32 ± 0.019	0.318TrpTyr: 0.318 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.294TyrAla: 2.294 ± 0.049	0.214TyrCys: 0.214 ± 0.012	1.224TyrAsp: 1.224 ± 0.035	1.069TyrGlu: 1.069 ± 0.037	0.751TyrPhe: 0.751 ± 0.032	2.003TyrGly: 2.003 ± 0.042	0.44TyrHis: 0.44 ± 0.022	0.669TyrIle: 0.669 ± 0.031	0.36TyrLys: 0.36 ± 0.019	2.319TyrLeu: 2.319 ± 0.051	0.332TyrMet: 0.332 ± 0.019	0.445TyrAsn: 0.445 ± 0.02	1.181TyrPro: 1.181 ± 0.039	0.692TyrGln: 0.692 ± 0.025	1.757TyrArg: 1.757 ± 0.043	1.273TyrSer: 1.273 ± 0.038	1.174TyrThr: 1.174 ± 0.036	1.566TyrVal: 1.566 ± 0.043	0.367TyrTrp: 0.367 ± 0.022	0.446TyrTyr: 0.446 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3247 proteins (964148 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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