Poinsettia latent virus (isolate Euphorbia pulcherrima/Germany/Siepen/2005) (PnLV) (Poinsettia cryptic virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polemovirus; Poinsettia latent virus

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5NDN0|P1_PNLV Genome-linked protein precursor OS=Poinsettia latent virus (isolate Euphorbia pulcherrima/Germany/Siepen/2005) OX=686943 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.61ALLGIKK7 pKa = 10.59LMTLVFAAWLSCCHH21 pKa = 6.58SSSALPSSGLSGPCLNHH38 pKa = 6.35SCLLRR43 pKa = 11.84NSLNGASQWGTILHH57 pKa = 6.05SPAVGSNCPPCPMMSIMGCSPPKK80 pKa = 9.61PLQSNSYY87 pKa = 10.36GVLCSTIASKK97 pKa = 10.91AKK99 pKa = 9.96QDD101 pKa = 5.61LKK103 pKa = 11.19LCWKK107 pKa = 9.5EE108 pKa = 3.66VQTRR112 pKa = 11.84SEE114 pKa = 4.34MYY116 pKa = 10.04SKK118 pKa = 10.61RR119 pKa = 11.84ISAALIDD126 pKa = 4.31SLHH129 pKa = 5.83QAVGMLLMIIIWIWSSIFLVVYY151 pKa = 10.12HH152 pKa = 6.09VLAYY156 pKa = 6.93MTTYY160 pKa = 10.05HH161 pKa = 7.06LSSAVCVGFLIFCTICAFRR180 pKa = 11.84LISWICGDD188 pKa = 4.31LLAFNVSGLTPIWVNFSEE206 pKa = 4.71SSCPAGLSLRR216 pKa = 11.84RR217 pKa = 11.84YY218 pKa = 9.1KK219 pKa = 10.7NEE221 pKa = 3.79KK222 pKa = 8.48TVEE225 pKa = 4.27GYY227 pKa = 10.53KK228 pKa = 10.52PFIIPQKK235 pKa = 9.65SPKK238 pKa = 9.83KK239 pKa = 10.38SVIEE243 pKa = 3.9LSFSNGSHH251 pKa = 7.02LGYY254 pKa = 9.18ATCVRR259 pKa = 11.84LWDD262 pKa = 4.04GSICLMTAKK271 pKa = 10.24HH272 pKa = 6.04CLVKK276 pKa = 10.43EE277 pKa = 4.0ALLKK281 pKa = 10.94GRR283 pKa = 11.84VAGHH287 pKa = 5.98SLPVKK292 pKa = 10.7NFDD295 pKa = 5.15LFLTCDD301 pKa = 3.84EE302 pKa = 5.01IDD304 pKa = 3.5FSLLRR309 pKa = 11.84GPKK312 pKa = 8.79QWEE315 pKa = 4.11AYY317 pKa = 10.15LGVKK321 pKa = 9.95GADD324 pKa = 3.58LITSNRR330 pKa = 11.84IGRR333 pKa = 11.84SPVTFYY339 pKa = 11.54NLSKK343 pKa = 10.83DD344 pKa = 4.16GEE346 pKa = 4.29WLANSAQITGRR357 pKa = 11.84HH358 pKa = 5.33GKK360 pKa = 9.48LCSVLSNTSPGDD372 pKa = 3.1SGTPYY377 pKa = 10.45YY378 pKa = 10.45SGKK381 pKa = 10.14NVVGIHH387 pKa = 6.49KK388 pKa = 7.92GTSEE392 pKa = 4.2LEE394 pKa = 4.23NYY396 pKa = 10.8NLMIPIPNIPGLTSPDD412 pKa = 3.41FKK414 pKa = 11.47FEE416 pKa = 4.12TTNVRR421 pKa = 11.84GNLYY425 pKa = 9.87NDD427 pKa = 3.0EE428 pKa = 4.22GFRR431 pKa = 11.84LSVGEE436 pKa = 4.21DD437 pKa = 3.15DD438 pKa = 5.91KK439 pKa = 12.05AEE441 pKa = 3.99HH442 pKa = 5.25WTDD445 pKa = 3.22RR446 pKa = 11.84LMKK449 pKa = 10.54SITFKK454 pKa = 9.45TKK456 pKa = 9.05RR457 pKa = 11.84WADD460 pKa = 3.16WAEE463 pKa = 4.13EE464 pKa = 3.97EE465 pKa = 5.29SEE467 pKa = 4.52SDD469 pKa = 3.59DD470 pKa = 3.46EE471 pKa = 4.6RR472 pKa = 11.84GKK474 pKa = 10.56VVPPAKK480 pKa = 9.57PSNYY484 pKa = 10.11GEE486 pKa = 4.57GCPPEE491 pKa = 4.34HH492 pKa = 6.2NQYY495 pKa = 11.09LSDD498 pKa = 4.4VGDD501 pKa = 4.51LLTKK505 pKa = 10.72VIGPEE510 pKa = 3.93QNEE513 pKa = 4.24KK514 pKa = 10.88CVDD517 pKa = 3.11ILMGIMGVDD526 pKa = 3.43KK527 pKa = 11.6NEE529 pKa = 4.08VAPHH533 pKa = 6.16KK534 pKa = 10.23EE535 pKa = 3.66EE536 pKa = 3.91KK537 pKa = 10.41AEE539 pKa = 3.98KK540 pKa = 9.71GKK542 pKa = 10.59RR543 pKa = 11.84SSGFGHH549 pKa = 6.44GKK551 pKa = 8.45NRR553 pKa = 11.84KK554 pKa = 7.14GTNHH558 pKa = 6.39PMRR561 pKa = 11.84RR562 pKa = 11.84GYY564 pKa = 10.6NFRR567 pKa = 11.84NCKK570 pKa = 9.96KK571 pKa = 10.75GGGQDD576 pKa = 3.01EE577 pKa = 4.91SEE579 pKa = 4.29SHH581 pKa = 6.53RR582 pKa = 11.84EE583 pKa = 3.46ISGRR587 pKa = 11.84DD588 pKa = 3.38PGRR591 pKa = 11.84EE592 pKa = 4.14SNDD595 pKa = 3.04KK596 pKa = 10.79SPQGEE601 pKa = 3.89AEE603 pKa = 3.75EE604 pKa = 4.24FEE606 pKa = 5.37RR607 pKa = 11.84YY608 pKa = 9.07FSSFYY613 pKa = 10.49SWKK616 pKa = 9.96LHH618 pKa = 6.1NSGEE622 pKa = 4.38ANSGFRR628 pKa = 11.84PCGKK632 pKa = 9.13IPKK635 pKa = 9.66FYY637 pKa = 10.38RR638 pKa = 11.84PRR640 pKa = 11.84KK641 pKa = 8.78RR642 pKa = 11.84RR643 pKa = 11.84VSEE646 pKa = 3.54WGQNLARR653 pKa = 11.84KK654 pKa = 9.15HH655 pKa = 6.01SSLGEE660 pKa = 3.73ITQGFGWPEE669 pKa = 3.48AGAEE673 pKa = 4.04AEE675 pKa = 4.15LRR677 pKa = 11.84SLRR680 pKa = 11.84LQAQRR685 pKa = 11.84WLEE688 pKa = 3.84RR689 pKa = 11.84SKK691 pKa = 11.51SSVIPSAIEE700 pKa = 3.64RR701 pKa = 11.84EE702 pKa = 4.27IVISRR707 pKa = 11.84LVEE710 pKa = 3.94SYY712 pKa = 10.48KK713 pKa = 9.93ICRR716 pKa = 11.84SEE718 pKa = 5.19APLCSSGSDD727 pKa = 3.62LSWKK731 pKa = 10.44GFLEE735 pKa = 4.19DD736 pKa = 3.46FRR738 pKa = 11.84EE739 pKa = 4.44AVSSLEE745 pKa = 3.78LDD747 pKa = 3.39AGIGVPYY754 pKa = 9.94IGYY757 pKa = 9.7GYY759 pKa = 6.91PTHH762 pKa = 7.0RR763 pKa = 11.84GWVEE767 pKa = 3.72NPRR770 pKa = 11.84LLPVLSRR777 pKa = 11.84LVYY780 pKa = 10.69ARR782 pKa = 11.84LQRR785 pKa = 11.84LATLSVDD792 pKa = 3.11GKK794 pKa = 9.08TPEE797 pKa = 3.92EE798 pKa = 4.07LVRR801 pKa = 11.84DD802 pKa = 3.98GLVDD806 pKa = 3.64PVRR809 pKa = 11.84VFVKK813 pKa = 10.77GEE815 pKa = 3.59PHH817 pKa = 6.19KK818 pKa = 10.54QSKK821 pKa = 10.21LDD823 pKa = 3.38EE824 pKa = 4.27GRR826 pKa = 11.84YY827 pKa = 8.96RR828 pKa = 11.84LIMSVSLIDD837 pKa = 3.51QLVARR842 pKa = 11.84VLFQKK847 pKa = 10.13QNKK850 pKa = 9.68LEE852 pKa = 4.19LLLWRR857 pKa = 11.84SIPSKK862 pKa = 10.52PGFGLSTVEE871 pKa = 3.97QVEE874 pKa = 4.17EE875 pKa = 4.9FIDD878 pKa = 3.42HH879 pKa = 7.41LARR882 pKa = 11.84VVDD885 pKa = 4.2VKK887 pKa = 11.37SDD889 pKa = 3.68DD890 pKa = 4.52LLEE893 pKa = 4.12NWRR896 pKa = 11.84EE897 pKa = 3.86LMVPTDD903 pKa = 3.69CSGFDD908 pKa = 3.34WSVSDD913 pKa = 4.32WMLKK917 pKa = 10.91DD918 pKa = 3.36EE919 pKa = 4.34MEE921 pKa = 4.09VRR923 pKa = 11.84NRR925 pKa = 11.84LTINCNDD932 pKa = 3.23LTRR935 pKa = 11.84RR936 pKa = 11.84LRR938 pKa = 11.84NSWLYY943 pKa = 10.75CLSNSDD949 pKa = 4.24LALSDD954 pKa = 3.77GSLLAQEE961 pKa = 4.54VPGVQKK967 pKa = 10.68SGSYY971 pKa = 8.27NTSSTNSRR979 pKa = 11.84IRR981 pKa = 11.84VMAAYY986 pKa = 9.45FAGASWAVAIGDD998 pKa = 4.3DD999 pKa = 3.91ALEE1002 pKa = 4.98SIDD1005 pKa = 3.67TTLAVYY1011 pKa = 10.38KK1012 pKa = 10.72SLGFKK1017 pKa = 10.76VEE1019 pKa = 3.91VSEE1022 pKa = 5.07DD1023 pKa = 3.72LEE1025 pKa = 4.48FCSHH1029 pKa = 6.22IFKK1032 pKa = 9.77TRR1034 pKa = 11.84SLAIPVNTSKK1044 pKa = 10.24MLYY1047 pKa = 9.31RR1048 pKa = 11.84LIYY1051 pKa = 10.06GYY1053 pKa = 10.57EE1054 pKa = 4.09PEE1056 pKa = 5.02CGNLDD1061 pKa = 3.42VLRR1064 pKa = 11.84NYY1066 pKa = 10.64LCALASVLHH1075 pKa = 6.07EE1076 pKa = 4.51LRR1078 pKa = 11.84HH1079 pKa = 6.23DD1080 pKa = 3.55QDD1082 pKa = 4.09LVQNLSKK1089 pKa = 10.44WLIPDD1094 pKa = 4.33GSQKK1098 pKa = 10.24ISS1100 pKa = 3.08

Molecular weight: 122.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q5NDM9|RDRP_PNLV Protein P2-P3 OS=Poinsettia latent virus (isolate Euphorbia pulcherrima/Germany/Siepen/2005) OX=686943 GN=ORF2/ORF3 PE=3 SV=2
MM1 pKa = 7.52AKK3 pKa = 10.28KK4 pKa = 10.22GVNKK8 pKa = 9.38QARR11 pKa = 11.84TNSKK15 pKa = 10.23LDD17 pKa = 3.36QLVTSLEE24 pKa = 4.07RR25 pKa = 11.84LTVGSMQRR33 pKa = 11.84KK34 pKa = 8.92SNRR37 pKa = 11.84QRR39 pKa = 11.84PKK41 pKa = 9.87SRR43 pKa = 11.84KK44 pKa = 6.78TSGRR48 pKa = 11.84VVTAPVSSAALIRR61 pKa = 11.84YY62 pKa = 7.55KK63 pKa = 10.82APSLNGNSMDD73 pKa = 4.08SVRR76 pKa = 11.84LASKK80 pKa = 10.47SLVIEE85 pKa = 3.96AVKK88 pKa = 10.84VGIEE92 pKa = 4.08YY93 pKa = 10.25QVSVVPLVPCIQHH106 pKa = 5.29TWLSGVACYY115 pKa = 8.8WSKK118 pKa = 11.04YY119 pKa = 6.48RR120 pKa = 11.84WRR122 pKa = 11.84SCRR125 pKa = 11.84LVYY128 pKa = 10.09IPYY131 pKa = 9.67CSSSFTGYY139 pKa = 10.88VSMGLSYY146 pKa = 10.82DD147 pKa = 3.66YY148 pKa = 11.71ADD150 pKa = 5.83AIPSDD155 pKa = 4.08EE156 pKa = 4.53TEE158 pKa = 3.76MSALKK163 pKa = 10.69GYY165 pKa = 6.35TTTSAWAGSEE175 pKa = 4.22GIKK178 pKa = 9.62MLSYY182 pKa = 8.7PTGAVPSGAVVLSLDD197 pKa = 4.2CASMSKK203 pKa = 9.83PWYY206 pKa = 8.78PYY208 pKa = 9.39ISAVAATNLGEE219 pKa = 4.28KK220 pKa = 10.23QPEE223 pKa = 4.43LLNQYY228 pKa = 8.72TPARR232 pKa = 11.84AFIGTGGGMQGVNGLTAGRR251 pKa = 11.84VFMVYY256 pKa = 9.97DD257 pKa = 3.63IEE259 pKa = 5.48LIEE262 pKa = 4.27PVSPAMQEE270 pKa = 4.16NLSSTGRR277 pKa = 11.84SKK279 pKa = 10.44IDD281 pKa = 3.29QSISPLTGADD291 pKa = 3.46FEE293 pKa = 4.62IVRR296 pKa = 11.84KK297 pKa = 10.07VSAISQIQQ305 pKa = 2.93

Molecular weight: 33.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2331

266

1100

582.8

64.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.049 ± 0.572

2.66 ± 0.348

4.462 ± 0.335

5.577 ± 0.776

3.175 ± 0.538

7.293 ± 0.429

1.931 ± 0.32

5.448 ± 0.284

6.006 ± 0.707

10.167 ± 0.609

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.233

4.161 ± 0.234

5.32 ± 0.316

2.574 ± 0.426

5.32 ± 0.558

10.854 ± 0.39

4.419 ± 0.505

6.821 ± 0.477

2.188 ± 0.124

3.346 ± 0.438
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski