Acetobacter orientalis
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2561 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5ZEC6|A0A2Z5ZEC6_9PROT Flagellar protein flbB OS=Acetobacter orientalis OX=146474 GN=Abor_017_092 PE=4 SV=1
MM1 pKa = 7.41 SVFNAISTAVSGINAQSTAFSNLSNNIANSQTVGYY36 pKa = 9.35 KK37 pKa = 10.06 ADD39 pKa = 3.41 STAFQDD45 pKa = 4.33 FVSGQLINSSRR56 pKa = 11.84 NEE58 pKa = 3.95 GNSDD62 pKa = 3.09 SVAAVTIQHH71 pKa = 5.95 VDD73 pKa = 3.08 KK74 pKa = 11.22 QGTATVSNDD83 pKa = 3.2 SLAMSIDD90 pKa = 3.2 GSGLFNVTKK99 pKa = 10.54 SPSDD103 pKa = 3.56 TSSSASLNQTRR114 pKa = 11.84 YY115 pKa = 5.18 YY116 pKa = 9.04 TRR118 pKa = 11.84 NGEE121 pKa = 4.08 FYY123 pKa = 10.34 EE124 pKa = 4.51 DD125 pKa = 3.41 KK126 pKa = 10.97 NGYY129 pKa = 8.78 LVNTSGYY136 pKa = 8.53 YY137 pKa = 9.71 LSGYY141 pKa = 9.82 KK142 pKa = 8.73 VTDD145 pKa = 3.3 QSTGTLSNQLTSVNVANVQYY165 pKa = 11.07 SPISNPTISVKK176 pKa = 10.67 GYY178 pKa = 9.78 VGSSVDD184 pKa = 3.48 KK185 pKa = 8.76 TTSTATNSQTTTIYY199 pKa = 10.98 DD200 pKa = 3.98 SNNDD204 pKa = 3.17 AHH206 pKa = 6.47 NVKK209 pKa = 10.35 LDD211 pKa = 3.22 WSYY214 pKa = 12.09 VSDD217 pKa = 3.51 STTTNNLQTWKK228 pKa = 10.38 LDD230 pKa = 3.18 VSTPDD235 pKa = 3.41 TNSSVSGTYY244 pKa = 9.53 YY245 pKa = 10.72 VQFDD249 pKa = 4.03 STTGAIQTVSKK260 pKa = 11.19 SEE262 pKa = 4.48 DD263 pKa = 3.42 GTNSLISTTNSSIPLTFNYY282 pKa = 9.84 TDD284 pKa = 4.29 LQSQSASMPFQLNLGLQGSTSGATLSSSTGPVITTPTTDD323 pKa = 3.24 SKK325 pKa = 11.6 SSSQVGSYY333 pKa = 9.72 TGVEE337 pKa = 3.99 IQSDD341 pKa = 4.16 GTIMAKK347 pKa = 10.74 FSLGDD352 pKa = 3.43 TQAIGQVALTNFASVNNLQEE372 pKa = 4.19 VSGQAYY378 pKa = 9.84 LATPSAGTPQTGLVGEE394 pKa = 4.68 NGTGSLTVGYY404 pKa = 8.3 TEE406 pKa = 5.81 SSTTDD411 pKa = 3.28 LTSDD415 pKa = 3.83 LSALIIAQQAYY426 pKa = 6.15 TANTKK431 pKa = 9.89 IVTTASEE438 pKa = 4.17 LLQTTIAMKK447 pKa = 10.31 QQ448 pKa = 3.01
Molecular weight: 46.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.888
IPC2_protein 4.151
IPC_protein 4.151
Toseland 3.935
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 3.973
Grimsley 3.846
Solomon 4.139
Lehninger 4.101
Nozaki 4.253
DTASelect 4.533
Thurlkill 3.986
EMBOSS 4.101
Sillero 4.279
Patrickios 1.278
IPC_peptide 4.139
IPC2_peptide 4.253
IPC2.peptide.svr19 4.138
Protein with the highest isoelectric point:
>tr|A0A252BCH2|A0A252BCH2_9PROT Magnesium transporter OS=Acetobacter orientalis OX=146474 GN=Abor_065_093 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 IIANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.47 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2561
0
2561
843554
42
3141
329.4
35.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.834 ± 0.068
1.028 ± 0.016
4.872 ± 0.035
5.03 ± 0.051
3.593 ± 0.032
8.164 ± 0.1
2.487 ± 0.025
4.807 ± 0.036
3.558 ± 0.036
10.479 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.421 ± 0.025
3.1 ± 0.04
5.52 ± 0.047
4.177 ± 0.033
5.98 ± 0.051
5.731 ± 0.051
6.298 ± 0.037
7.159 ± 0.039
1.357 ± 0.02
2.406 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here