Polaribacter sp. ZY113

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2J8X0|A0A2U2J8X0_9FLAO Uncharacterized protein OS=Polaribacter sp. ZY113 OX=2100726 GN=DIS07_09955 PE=4 SV=1
MM1 pKa = 7.38QDD3 pKa = 2.87INIKK7 pKa = 8.58ITDD10 pKa = 3.97RR11 pKa = 11.84NGVTHH16 pKa = 7.19DD17 pKa = 3.68VVAPTDD23 pKa = 3.34MAMNLMEE30 pKa = 4.23VVRR33 pKa = 11.84SYY35 pKa = 11.48EE36 pKa = 3.81LAEE39 pKa = 4.4EE40 pKa = 4.51GTIGICGGMAMCASCQCYY58 pKa = 10.11VKK60 pKa = 10.41SDD62 pKa = 3.69HH63 pKa = 6.7EE64 pKa = 4.45LPEE67 pKa = 4.04MTDD70 pKa = 4.64DD71 pKa = 3.87EE72 pKa = 4.82DD73 pKa = 6.08AMLAEE78 pKa = 4.68AFNVEE83 pKa = 4.34DD84 pKa = 4.43NSRR87 pKa = 11.84LGCQIQMTPEE97 pKa = 3.81MEE99 pKa = 4.16GLEE102 pKa = 4.16VEE104 pKa = 4.95LAPEE108 pKa = 4.07SS109 pKa = 3.78

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2JDA1|A0A2U2JDA1_9FLAO Phosphoesterase OS=Polaribacter sp. ZY113 OX=2100726 GN=DIS07_07120 PE=4 SV=1
MM1 pKa = 6.94MVSTVEE7 pKa = 4.06ANNGNNLPNRR17 pKa = 11.84IGFNYY22 pKa = 10.15SYY24 pKa = 10.71QNSVNFVEE32 pKa = 4.64RR33 pKa = 11.84GIEE36 pKa = 4.07FFVFTNGEE44 pKa = 3.78FDD46 pKa = 4.63FDD48 pKa = 4.03TNYY51 pKa = 10.69NSVYY55 pKa = 10.1FDD57 pKa = 3.47YY58 pKa = 10.97NGRR61 pKa = 11.84RR62 pKa = 11.84TRR64 pKa = 11.84RR65 pKa = 11.84NNGVSIDD72 pKa = 3.4RR73 pKa = 11.84DD74 pKa = 3.19FRR76 pKa = 11.84GRR78 pKa = 11.84VRR80 pKa = 11.84RR81 pKa = 11.84IGGTFINYY89 pKa = 9.03DD90 pKa = 2.98FRR92 pKa = 11.84GNVTRR97 pKa = 11.84IGNVFMRR104 pKa = 11.84YY105 pKa = 8.99HH106 pKa = 7.33RR107 pKa = 11.84GRR109 pKa = 11.84LTKK112 pKa = 10.44VGNLRR117 pKa = 11.84VRR119 pKa = 11.84YY120 pKa = 9.46DD121 pKa = 2.8RR122 pKa = 11.84WGYY125 pKa = 7.8PAFYY129 pKa = 8.79GTVRR133 pKa = 11.84NNIYY137 pKa = 7.98THH139 pKa = 6.05NGVRR143 pKa = 11.84FNLNIGDD150 pKa = 3.57ICDD153 pKa = 3.46YY154 pKa = 10.2NDD156 pKa = 3.25RR157 pKa = 11.84YY158 pKa = 10.73FFGRR162 pKa = 11.84EE163 pKa = 3.47FRR165 pKa = 11.84SNYY168 pKa = 8.46SQIRR172 pKa = 11.84EE173 pKa = 3.96DD174 pKa = 3.3RR175 pKa = 11.84NYY177 pKa = 11.03YY178 pKa = 9.15YY179 pKa = 11.34YY180 pKa = 11.05KK181 pKa = 10.9ANTNAKK187 pKa = 8.95IGKK190 pKa = 9.19RR191 pKa = 11.84SQILRR196 pKa = 11.84RR197 pKa = 11.84KK198 pKa = 9.51KK199 pKa = 9.77PGSKK203 pKa = 9.65RR204 pKa = 11.84IEE206 pKa = 3.88NSSIKK211 pKa = 10.16RR212 pKa = 11.84KK213 pKa = 10.0RR214 pKa = 11.84NNSYY218 pKa = 10.5RR219 pKa = 11.84KK220 pKa = 8.58PASAVNSKK228 pKa = 10.67RR229 pKa = 11.84KK230 pKa = 8.98IKK232 pKa = 10.11KK233 pKa = 9.62DD234 pKa = 3.06RR235 pKa = 11.84RR236 pKa = 11.84LSTNRR241 pKa = 11.84NSSATYY247 pKa = 10.23RR248 pKa = 11.84KK249 pKa = 9.71LDD251 pKa = 3.4NSKK254 pKa = 10.96RR255 pKa = 11.84EE256 pKa = 3.85VNKK259 pKa = 9.58TIKK262 pKa = 10.21RR263 pKa = 11.84SSDD266 pKa = 2.96RR267 pKa = 11.84KK268 pKa = 9.51EE269 pKa = 4.11KK270 pKa = 10.24IVKK273 pKa = 9.55KK274 pKa = 10.43RR275 pKa = 11.84RR276 pKa = 11.84NN277 pKa = 3.27

Molecular weight: 33.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2936

0

2936

1035397

38

2926

352.7

39.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.779 ± 0.045

0.698 ± 0.013

5.432 ± 0.046

6.276 ± 0.045

5.571 ± 0.046

6.241 ± 0.052

1.683 ± 0.02

8.442 ± 0.044

9.082 ± 0.074

8.943 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.024

6.82 ± 0.05

3.153 ± 0.025

3.17 ± 0.022

3.241 ± 0.029

6.628 ± 0.038

5.729 ± 0.049

6.038 ± 0.032

1.072 ± 0.017

4.003 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski