Salmonella phage SeSz-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BGZ0|A0A411BGZ0_9CAUD Uncharacterized protein OS=Salmonella phage SeSz-2 OX=2419753 GN=SeSz2_79 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 9.76PQEE5 pKa = 3.91ATLAIKK11 pKa = 10.42DD12 pKa = 3.94ILSQCNSDD20 pKa = 3.57DD21 pKa = 3.86DD22 pKa = 4.46FRR24 pKa = 11.84VAMYY28 pKa = 9.64CIIEE32 pKa = 4.3FMLISDD38 pKa = 5.05LSIKK42 pKa = 10.46DD43 pKa = 3.48IISCIEE49 pKa = 3.9NAGGDD54 pKa = 3.87YY55 pKa = 10.99VEE57 pKa = 5.22SDD59 pKa = 3.22RR60 pKa = 11.84CIDD63 pKa = 4.78DD64 pKa = 4.32MIAEE68 pKa = 4.56LKK70 pKa = 10.84DD71 pKa = 3.33NYY73 pKa = 10.98LL74 pKa = 3.67

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BHP1|A0A411BHP1_9CAUD Uncharacterized protein OS=Salmonella phage SeSz-2 OX=2419753 GN=SeSz2_59 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84KK3 pKa = 9.85FSTLVDD9 pKa = 5.35LIFADD14 pKa = 3.46ISEE17 pKa = 4.59DD18 pKa = 3.24NARR21 pKa = 11.84SKK23 pKa = 10.83KK24 pKa = 10.25SRR26 pKa = 11.84NQRR29 pKa = 11.84QDD31 pKa = 2.32ITFRR35 pKa = 11.84TLLKK39 pKa = 10.45QSMRR43 pKa = 11.84SKK45 pKa = 10.83KK46 pKa = 6.74EE47 pKa = 3.09WRR49 pKa = 11.84YY50 pKa = 10.03RR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.05RR54 pKa = 11.84VLIRR58 pKa = 11.84LCKK61 pKa = 10.57LNMNKK66 pKa = 9.42ILKK69 pKa = 10.54DD70 pKa = 3.22MTKK73 pKa = 10.79

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

12773

47

829

163.8

18.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.753 ± 0.588

1.441 ± 0.184

6.381 ± 0.301

6.929 ± 0.29

3.343 ± 0.197

6.952 ± 0.233

1.581 ± 0.165

6.897 ± 0.192

6.6 ± 0.305

6.937 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.335 ± 0.182

4.721 ± 0.238

3.382 ± 0.226

3.789 ± 0.247

5.332 ± 0.238

6.428 ± 0.287

5.527 ± 0.445

6.506 ± 0.271

1.566 ± 0.128

3.601 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski