Caldithrix abyssi DSM 13497
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3674 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H1XP64|H1XP64_9BACT Amino acid adenylation domain protein OS=Caldithrix abyssi DSM 13497 OX=880073 GN=Cabys_1398 PE=4 SV=1
MM1 pKa = 7.14 KK2 pKa = 9.69 TRR4 pKa = 11.84 IVILILGLATAILATTYY21 pKa = 8.31 HH22 pKa = 6.33 TLPFNGQNAFAPDD35 pKa = 3.55 EE36 pKa = 4.37 DD37 pKa = 5.7 FITSTDD43 pKa = 3.3 TVLAYY48 pKa = 7.5 CTWDD52 pKa = 3.35 DD53 pKa = 3.41 QYY55 pKa = 11.93 LYY57 pKa = 10.74 LGLSSPFLATPNDD70 pKa = 4.05 TARR73 pKa = 11.84 GRR75 pKa = 11.84 YY76 pKa = 9.58 DD77 pKa = 3.49 SFWYY81 pKa = 9.87 IDD83 pKa = 3.96 TDD85 pKa = 3.59 PHH87 pKa = 6.64 PDD89 pKa = 3.38 NPKK92 pKa = 10.53 SGLGTDD98 pKa = 3.22 QTGSYY103 pKa = 9.68 FIQINTSQPWWFDD116 pKa = 3.42 EE117 pKa = 4.41 QSWEE121 pKa = 4.11 LPFFADD127 pKa = 3.23 YY128 pKa = 10.84 RR129 pKa = 11.84 IIPRR133 pKa = 11.84 YY134 pKa = 7.97 YY135 pKa = 10.72 KK136 pKa = 10.08 KK137 pKa = 10.75 DD138 pKa = 3.14 SVYY141 pKa = 11.3 ASFYY145 pKa = 11.06 YY146 pKa = 10.4 YY147 pKa = 10.82 DD148 pKa = 3.83 SDD150 pKa = 3.68 SARR153 pKa = 11.84 WFFRR157 pKa = 11.84 GDD159 pKa = 2.96 IDD161 pKa = 4.45 TSLANLNYY169 pKa = 9.37 IDD171 pKa = 4.74 GYY173 pKa = 10.74 YY174 pKa = 10.5 EE175 pKa = 4.07 LRR177 pKa = 11.84 LPLDD181 pKa = 3.62 SLNFPTDD188 pKa = 3.32 INILGYY194 pKa = 10.39 SVDD197 pKa = 4.21 GQWEE201 pKa = 4.15 SDD203 pKa = 3.64 IYY205 pKa = 10.84 WDD207 pKa = 4.72 DD208 pKa = 3.64 EE209 pKa = 4.67 YY210 pKa = 11.73 GFTRR214 pKa = 11.84 DD215 pKa = 3.09 VGGTFASWPWSSLVGGDD232 pKa = 3.98 GDD234 pKa = 4.06 YY235 pKa = 9.27 HH236 pKa = 5.9 TKK238 pKa = 10.8 GKK240 pKa = 9.79 FNHH243 pKa = 6.72 WFHH246 pKa = 6.76 FHH248 pKa = 6.54 LQPGISPDD256 pKa = 3.64 QEE258 pKa = 3.82 NDD260 pKa = 3.45 PPVASPITGQTINEE274 pKa = 4.32 GEE276 pKa = 4.33 SFAVIDD282 pKa = 4.05 LNSYY286 pKa = 10.37 VFDD289 pKa = 5.45 DD290 pKa = 4.5 LTPDD294 pKa = 3.45 TLLIWTTEE302 pKa = 4.2 TIDD305 pKa = 5.48 LIVTIQDD312 pKa = 3.35 SNQAQVSTPNPNWNGTEE329 pKa = 4.08 TITFIVTDD337 pKa = 4.04 EE338 pKa = 4.51 GGKK341 pKa = 10.03 SDD343 pKa = 3.69 TTTGTFTMLGDD354 pKa = 3.61 NQIPVAVNDD363 pKa = 3.6 SSNTIEE369 pKa = 4.19 EE370 pKa = 4.04 QSVSINLTANDD381 pKa = 3.81 SDD383 pKa = 4.31 ADD385 pKa = 3.92 NDD387 pKa = 4.01 SLHH390 pKa = 7.2 IDD392 pKa = 4.85 RR393 pKa = 11.84 ILTTNNGTVVIDD405 pKa = 3.62 NDD407 pKa = 3.85 SMVTYY412 pKa = 9.28 TPNVDD417 pKa = 4.01 FHH419 pKa = 6.39 GTDD422 pKa = 2.86 SFEE425 pKa = 4.39 YY426 pKa = 10.85 VVTDD430 pKa = 3.68 GNGGRR435 pKa = 11.84 DD436 pKa = 3.23 TATVFVTVDD445 pKa = 3.25 NRR447 pKa = 11.84 NDD449 pKa = 3.32 PPEE452 pKa = 4.39 IVNLPDD458 pKa = 4.06 VINMTTNDD466 pKa = 3.38 STKK469 pKa = 10.71 LYY471 pKa = 9.72 MMDD474 pKa = 3.13 YY475 pKa = 10.21 AYY477 pKa = 10.61 DD478 pKa = 3.54 VDD480 pKa = 4.52 TPDD483 pKa = 5.8 SLLTWSFSVNDD494 pKa = 3.87 PAISYY499 pKa = 10.45 AYY501 pKa = 10.25 DD502 pKa = 3.24 QTTDD506 pKa = 2.78 TLTIYY511 pKa = 10.41 SHH513 pKa = 7.2 EE514 pKa = 4.48 INGDD518 pKa = 3.65 FYY520 pKa = 11.69 LFTTLTDD527 pKa = 3.75 DD528 pKa = 5.31 SGASDD533 pKa = 3.77 QDD535 pKa = 3.95 TITIRR540 pKa = 11.84 VSGTSALDD548 pKa = 3.6 LRR550 pKa = 11.84 STAIPDD556 pKa = 3.42 QFTVLQNFPNPFNPSTHH573 pKa = 6.47 LAFGLTKK580 pKa = 10.65 SSDD583 pKa = 3.48 VQIEE587 pKa = 3.94 IFNILGQRR595 pKa = 11.84 VFSKK599 pKa = 10.59 RR600 pKa = 11.84 LSGLSAGFHH609 pKa = 4.98 TVKK612 pKa = 10.24 VDD614 pKa = 3.41 AARR617 pKa = 11.84 WPAGIYY623 pKa = 10.06 LYY625 pKa = 10.41 RR626 pKa = 11.84 ISAEE630 pKa = 3.55 NTMVIKK636 pKa = 10.85 KK637 pKa = 8.83 MVLVRR642 pKa = 4.15
Molecular weight: 72.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.872
IPC_protein 3.935
Toseland 3.694
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.088
Wikipedia 3.897
Rodwell 3.757
Grimsley 3.605
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.355
Thurlkill 3.757
EMBOSS 3.91
Sillero 4.062
Patrickios 1.558
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|H1XPH5|H1XPH5_9BACT Uncharacterized protein OS=Caldithrix abyssi DSM 13497 OX=880073 GN=Cabys_2709 PE=4 SV=1
MM1 pKa = 7.32 PCGRR5 pKa = 11.84 KK6 pKa = 9.29 RR7 pKa = 11.84 KK8 pKa = 7.42 RR9 pKa = 11.84 AKK11 pKa = 9.56 MATHH15 pKa = 6.33 KK16 pKa = 10.31 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.16 NRR25 pKa = 11.84 HH26 pKa = 4.6 KK27 pKa = 10.96 KK28 pKa = 10.03 KK29 pKa = 10.26 IRR31 pKa = 3.43
Molecular weight: 3.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.789
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.398
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.135
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.011
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3674
0
3674
1397650
31
4710
380.4
43.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.103 ± 0.04
0.686 ± 0.013
5.294 ± 0.034
6.622 ± 0.044
5.251 ± 0.031
6.486 ± 0.038
2.016 ± 0.02
7.353 ± 0.035
6.686 ± 0.043
10.331 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.213 ± 0.016
4.655 ± 0.032
4.136 ± 0.025
4.229 ± 0.029
5.096 ± 0.033
5.522 ± 0.031
4.81 ± 0.032
6.439 ± 0.031
1.249 ± 0.017
3.823 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here