Actinomyces ruminicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9RVC3|A0A1G9RVC3_9ACTO Oligo-1 6-glucosidase OS=Actinomyces ruminicola OX=332524 GN=SAMN04487766_101164 PE=3 SV=1
MM1 pKa = 7.69SIPSPALLRR10 pKa = 11.84RR11 pKa = 11.84SGAVVAGSALLLGSVALGPAAVADD35 pKa = 3.71IDD37 pKa = 4.86DD38 pKa = 4.52GVNSSVHH45 pKa = 5.98IDD47 pKa = 3.6LVLDD51 pKa = 3.7EE52 pKa = 6.11DD53 pKa = 4.04KK54 pKa = 10.7TADD57 pKa = 3.62MIMSIGIDD65 pKa = 4.27GITPDD70 pKa = 4.66LYY72 pKa = 10.42CTEE75 pKa = 4.52DD76 pKa = 3.3VLEE79 pKa = 4.26ISEE82 pKa = 4.4LKK84 pKa = 10.78YY85 pKa = 10.7IEE87 pKa = 5.71DD88 pKa = 4.36ADD90 pKa = 4.36DD91 pKa = 5.71DD92 pKa = 4.14IAVTLAAEE100 pKa = 4.65GNSCVLTMSDD110 pKa = 4.03LPVVGSTDD118 pKa = 3.01EE119 pKa = 4.56DD120 pKa = 4.03FTVKK124 pKa = 10.4HH125 pKa = 5.8QDD127 pKa = 2.34GTYY130 pKa = 8.64IVKK133 pKa = 10.19IAGFTDD139 pKa = 4.16FAEE142 pKa = 4.57YY143 pKa = 10.43DD144 pKa = 3.78SSTMTITFPGEE155 pKa = 3.79VIEE158 pKa = 4.48ADD160 pKa = 3.49EE161 pKa = 4.19NAEE164 pKa = 4.06VSGKK168 pKa = 8.83EE169 pKa = 3.85ASWEE173 pKa = 4.07NIAEE177 pKa = 4.33LDD179 pKa = 3.83SLQATGWDD187 pKa = 3.96SADD190 pKa = 4.07HH191 pKa = 6.19PVSTPSASAEE201 pKa = 4.11PSSSPAATEE210 pKa = 3.6PAAAQAADD218 pKa = 4.2EE219 pKa = 4.54DD220 pKa = 4.76SKK222 pKa = 10.24TDD224 pKa = 3.41EE225 pKa = 4.33SSGSGLLPWIILTLAVLVAIVGGIIAAVVARR256 pKa = 11.84SRR258 pKa = 11.84RR259 pKa = 11.84KK260 pKa = 9.32AAAPALGYY268 pKa = 7.06PTGYY272 pKa = 10.01QPGYY276 pKa = 9.12QAQPYY281 pKa = 7.72PQQPGQPQPHH291 pKa = 5.43QQPGPQAYY299 pKa = 8.08GAPQQPYY306 pKa = 8.04QQPGYY311 pKa = 8.25PQQPGQPQPGQGQQPNSPNGQYY333 pKa = 11.18

Molecular weight: 34.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0DT50|A0A1H0DT50_9ACTO Predicted transcriptional regulator ArsR family OS=Actinomyces ruminicola OX=332524 GN=SAMN04487766_106134 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84HH12 pKa = 5.28RR13 pKa = 11.84AKK15 pKa = 10.38VHH17 pKa = 5.26GFRR20 pKa = 11.84KK21 pKa = 9.9RR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2725

0

2725

958828

40

3188

351.9

37.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.083 ± 0.071

0.768 ± 0.012

6.167 ± 0.042

5.541 ± 0.046

2.601 ± 0.03

8.981 ± 0.041

1.981 ± 0.023

3.901 ± 0.036

1.74 ± 0.03

10.1 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.958 ± 0.021

1.983 ± 0.025

5.659 ± 0.038

2.881 ± 0.027

7.509 ± 0.062

5.518 ± 0.037

6.309 ± 0.052

8.723 ± 0.042

1.503 ± 0.02

2.094 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski