Wuhan arthropod virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KM75|A0A1L3KM75_9VIRU Calici_coat domain-containing protein OS=Wuhan arthropod virus 3 OX=1923692 PE=4 SV=1
MM1 pKa = 6.94STQTASAVSMGDD13 pKa = 3.23EE14 pKa = 4.32VFSSTEE20 pKa = 3.83DD21 pKa = 3.24APLPVSAIQAEE32 pKa = 4.39HH33 pKa = 7.24ADD35 pKa = 3.61PGEE38 pKa = 4.35AVPHH42 pKa = 5.7VGAANTIDD50 pKa = 3.46PFFYY54 pKa = 10.46EE55 pKa = 4.02QFVAQTNFTWTSSDD69 pKa = 3.47NPGKK73 pKa = 10.39LLYY76 pKa = 8.82TIPIHH81 pKa = 6.5PVFANIYY88 pKa = 9.38LAYY91 pKa = 10.36LVQLYY96 pKa = 7.27NTWVGGLDD104 pKa = 3.6FKK106 pKa = 11.66VKK108 pKa = 9.85VAGTGFHH115 pKa = 6.94AGALILARR123 pKa = 11.84IPPNIDD129 pKa = 3.2PNSLTTTSAITAFPCTFIDD148 pKa = 4.45PKK150 pKa = 10.31TLTAASQSIMDD161 pKa = 3.87QRR163 pKa = 11.84QLMYY167 pKa = 10.36HH168 pKa = 4.75YY169 pKa = 9.5TNYY172 pKa = 10.92DD173 pKa = 3.44SADD176 pKa = 3.32STTIGGHH183 pKa = 4.62FAIYY187 pKa = 10.2VLQSLNTSASGTNQIDD203 pKa = 3.58VQVFCKK209 pKa = 10.53ASADD213 pKa = 3.9FEE215 pKa = 4.52LLQIKK220 pKa = 9.32PPNLTLNPPTPTYY233 pKa = 11.69ANFTKK238 pKa = 10.75LFDD241 pKa = 3.96RR242 pKa = 11.84LAGQTTPYY250 pKa = 10.98AFGSNCIEE258 pKa = 4.8FIVAPTTGPIYY269 pKa = 10.69AGLEE273 pKa = 4.02GARR276 pKa = 11.84QLDD279 pKa = 3.93GSLLGTYY286 pKa = 7.36TTFDD290 pKa = 3.58EE291 pKa = 4.5QVTAQRR297 pKa = 11.84FSGISGVAGTNAGGKK312 pKa = 9.58LVIHH316 pKa = 6.93RR317 pKa = 11.84NAAAPYY323 pKa = 7.91TFQVIQFYY331 pKa = 11.04QNNCKK336 pKa = 8.5VTGVAMHH343 pKa = 7.38AIGDD347 pKa = 3.88EE348 pKa = 4.31STPASYY354 pKa = 10.94VSSVDD359 pKa = 3.11VDD361 pKa = 3.79FTLPYY366 pKa = 9.73NASSTAALGYY376 pKa = 9.19MDD378 pKa = 4.99EE379 pKa = 4.53SSATFVLTDD388 pKa = 4.49GINPVVIPTQGEE400 pKa = 4.42SLVGFGFYY408 pKa = 9.56ATRR411 pKa = 11.84SIAGSIYY418 pKa = 9.24YY419 pKa = 7.91TAQSTQMALGLRR431 pKa = 11.84DD432 pKa = 3.6KK433 pKa = 11.34VFGEE437 pKa = 4.38VPPDD441 pKa = 3.4SALLFILIDD450 pKa = 3.82TVLSIPVNYY459 pKa = 10.27LKK461 pKa = 10.73LYY463 pKa = 8.05PQGFFTSSHH472 pKa = 5.27VDD474 pKa = 3.16QPIVYY479 pKa = 9.97DD480 pKa = 3.36SRR482 pKa = 11.84RR483 pKa = 11.84YY484 pKa = 8.42RR485 pKa = 11.84LEE487 pKa = 3.64YY488 pKa = 10.35VGLIEE493 pKa = 4.41VTTTVPTKK501 pKa = 10.45IEE503 pKa = 4.18FEE505 pKa = 4.24THH507 pKa = 5.43KK508 pKa = 10.79LAMSRR513 pKa = 11.84SFDD516 pKa = 3.53HH517 pKa = 6.3KK518 pKa = 10.46KK519 pKa = 10.12KK520 pKa = 10.67RR521 pKa = 11.84SGFLSAAYY529 pKa = 9.71HH530 pKa = 6.2GEE532 pKa = 4.15SAAA535 pKa = 4.04

Molecular weight: 57.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KM75|A0A1L3KM75_9VIRU Calici_coat domain-containing protein OS=Wuhan arthropod virus 3 OX=1923692 PE=4 SV=1
MM1 pKa = 7.43GVLSLATKK9 pKa = 9.32VGKK12 pKa = 9.9VAAKK16 pKa = 10.27GATTVNRR23 pKa = 11.84VVVNNGLPNTEE34 pKa = 3.52KK35 pKa = 10.64RR36 pKa = 11.84ISRR39 pKa = 11.84RR40 pKa = 11.84AARR43 pKa = 11.84YY44 pKa = 7.17EE45 pKa = 3.65RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 10.67AKK50 pKa = 10.58GKK52 pKa = 8.55FTTNLGAKK60 pKa = 8.53SAHH63 pKa = 5.62YY64 pKa = 10.45KK65 pKa = 10.31EE66 pKa = 5.55GIDD69 pKa = 3.25IAKK72 pKa = 8.94TKK74 pKa = 11.0GEE76 pKa = 3.99ASVFSQKK83 pKa = 10.22YY84 pKa = 9.3KK85 pKa = 10.41KK86 pKa = 9.99AAPKK90 pKa = 9.85KK91 pKa = 10.17APNKK95 pKa = 10.24HH96 pKa = 5.09LVKK99 pKa = 9.61TKK101 pKa = 10.61KK102 pKa = 9.6VVKK105 pKa = 10.0PITKK109 pKa = 8.25VTNPKK114 pKa = 10.38SLLKK118 pKa = 9.63PGNMGVAKK126 pKa = 10.38AITKK130 pKa = 8.91AVPVKK135 pKa = 10.47SVAKK139 pKa = 9.05VAPVKK144 pKa = 10.39PLTKK148 pKa = 10.06VASSTTKK155 pKa = 10.28KK156 pKa = 10.61SVVKK160 pKa = 10.73APIQSNSKK168 pKa = 10.38FDD170 pKa = 4.42DD171 pKa = 4.01PLKK174 pKa = 10.9KK175 pKa = 10.46GFPSNSTGAAGVKK188 pKa = 9.75PPPPKK193 pKa = 10.46DD194 pKa = 3.26LGLVKK199 pKa = 8.92PTPAKK204 pKa = 10.38VLSSATKK211 pKa = 9.97EE212 pKa = 3.98KK213 pKa = 9.97FTIPRR218 pKa = 11.84PTLVTKK224 pKa = 10.29AKK226 pKa = 10.04PVAKK230 pKa = 8.51PTAVVPPTKK239 pKa = 10.22VVKK242 pKa = 10.14PPAKK246 pKa = 10.15AGTPTTPAKK255 pKa = 10.97VNTPVKK261 pKa = 10.26KK262 pKa = 9.4ITDD265 pKa = 3.66VVPSKK270 pKa = 10.58GTVGKK275 pKa = 10.14IDD277 pKa = 4.88DD278 pKa = 4.49LTPKK282 pKa = 10.34PKK284 pKa = 10.55LKK286 pKa = 10.66GMAKK290 pKa = 9.76YY291 pKa = 9.46QALAKK296 pKa = 8.82KK297 pKa = 9.56HH298 pKa = 4.9KK299 pKa = 9.13WKK301 pKa = 9.98IAKK304 pKa = 9.74AVGGGTLSAVGATAMGLLGYY324 pKa = 7.56QAKK327 pKa = 9.71VAAANIAKK335 pKa = 10.13NSNRR339 pKa = 11.84EE340 pKa = 3.91TLIYY344 pKa = 10.68DD345 pKa = 3.54ATKK348 pKa = 10.83SSAKK352 pKa = 9.98YY353 pKa = 10.14AINEE357 pKa = 4.06KK358 pKa = 10.85EE359 pKa = 4.08EE360 pKa = 3.96AQNAAKK366 pKa = 10.17AQKK369 pKa = 9.72QRR371 pKa = 11.84EE372 pKa = 3.94EE373 pKa = 4.37DD374 pKa = 3.56LANTATTNKK383 pKa = 9.73HH384 pKa = 5.75LEE386 pKa = 4.51AIANKK391 pKa = 7.8PTLASMLTTKK401 pKa = 10.15RR402 pKa = 11.84QKK404 pKa = 11.16SNIRR408 pKa = 11.84YY409 pKa = 8.68QVGNNQVSRR418 pKa = 11.84NPLRR422 pKa = 11.84KK423 pKa = 8.48VTNSRR428 pKa = 11.84PQIDD432 pKa = 3.7KK433 pKa = 10.65LAPEE437 pKa = 4.41PSFLDD442 pKa = 3.36SLRR445 pKa = 11.84FKK447 pKa = 11.53SNMVV451 pKa = 3.13

Molecular weight: 48.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3229

451

2243

1076.3

119.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.773 ± 1.931

1.61 ± 0.661

6.039 ± 1.27

5.048 ± 1.019

4.614 ± 0.813

5.915 ± 0.329

2.137 ± 0.364

5.141 ± 0.424

7.371 ± 2.516

8.455 ± 0.829

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.449

4.645 ± 0.255

6.38 ± 0.505

3.314 ± 0.331

3.716 ± 0.427

6.906 ± 0.358

6.689 ± 1.441

6.999 ± 0.69

1.022 ± 0.383

3.964 ± 0.581
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski