Tacheng Tick Virus 4
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5KT91|A0A0B5KT91_9VIRU GDP polyribonucleotidyltransferase OS=Tacheng Tick Virus 4 OX=1608086 GN=L PE=4 SV=1
MM1 pKa = 7.47 TEE3 pKa = 3.35 QAAIDD8 pKa = 4.2 LRR10 pKa = 11.84 DD11 pKa = 3.64 HH12 pKa = 6.47 PSVYY16 pKa = 10.07 RR17 pKa = 11.84 RR18 pKa = 11.84 TIIVGAEE25 pKa = 3.55 EE26 pKa = 3.7 RR27 pKa = 11.84 AVRR30 pKa = 11.84 RR31 pKa = 11.84 FATQNPAPGSAPSPQQQYY49 pKa = 11.22 KK50 pKa = 10.11 DD51 pKa = 3.7 LHH53 pKa = 7.86 SGDD56 pKa = 4.66 LVNADD61 pKa = 3.65 TVTRR65 pKa = 11.84 LYY67 pKa = 10.03 VTDD70 pKa = 3.35 TDD72 pKa = 3.57 NPEE75 pKa = 3.83 RR76 pKa = 11.84 NKK78 pKa = 9.94 TIRR81 pKa = 11.84 VGPMMFEE88 pKa = 4.13 RR89 pKa = 11.84 NIEE92 pKa = 4.06 HH93 pKa = 7.1 AFDD96 pKa = 5.05 FYY98 pKa = 11.65 SEE100 pKa = 4.06 GMKK103 pKa = 10.33 FAMPRR108 pKa = 11.84 ASYY111 pKa = 9.9 PPYY114 pKa = 10.79 DD115 pKa = 5.0 RR116 pKa = 11.84 ISSQHH121 pKa = 7.27 LEE123 pKa = 4.38 IVWAAAVAANMGQEE137 pKa = 4.16 VPDD140 pKa = 4.25 DD141 pKa = 3.59 QFNWMVVGQAFAVGLPDD158 pKa = 3.29 HH159 pKa = 6.73 VFYY162 pKa = 9.84 EE163 pKa = 4.74 TIRR166 pKa = 11.84 ANEE169 pKa = 3.78 IVLAGARR176 pKa = 11.84 TMLQKK181 pKa = 9.72 MQEE184 pKa = 4.12 PARR187 pKa = 11.84 TWLLRR192 pKa = 11.84 RR193 pKa = 11.84 SGNADD198 pKa = 2.96 WAVLNSQVEE207 pKa = 4.3 MPPEE211 pKa = 5.05 GIPMVLEE218 pKa = 4.2 ANALHH223 pKa = 6.66 LSLGGRR229 pKa = 11.84 ARR231 pKa = 11.84 MMEE234 pKa = 4.11 AYY236 pKa = 9.39 MGIFLAYY243 pKa = 7.52 TQQGNITVQKK253 pKa = 9.34 LDD255 pKa = 4.37 KK256 pKa = 10.26 IIKK259 pKa = 9.8 QIAEE263 pKa = 3.97 VQINVALTPANINTLWAKK281 pKa = 10.02 FGPFFNEE288 pKa = 3.09 EE289 pKa = 3.24 AAAYY293 pKa = 7.37 FFPRR297 pKa = 11.84 WADD300 pKa = 3.57 LFADD304 pKa = 3.74 RR305 pKa = 11.84 HH306 pKa = 5.11 IRR308 pKa = 11.84 FRR310 pKa = 11.84 LIFVQAANAGLTGLTLVRR328 pKa = 11.84 DD329 pKa = 4.02 MLALYY334 pKa = 9.33 PKK336 pKa = 10.17 FPWHH340 pKa = 6.66 KK341 pKa = 10.35 LAAQYY346 pKa = 9.24 PAEE349 pKa = 3.9 WQAFLKK355 pKa = 10.77 ADD357 pKa = 3.57 ATVGTNVYY365 pKa = 10.07 YY366 pKa = 10.83 GYY368 pKa = 9.07 TRR370 pKa = 11.84 DD371 pKa = 3.55 LGIVSVRR378 pKa = 11.84 NYY380 pKa = 10.57 VPIVYY385 pKa = 9.49 CAKK388 pKa = 9.4 QLRR391 pKa = 11.84 EE392 pKa = 4.21 KK393 pKa = 11.07 VHH395 pKa = 6.84 GDD397 pKa = 3.15 QNMQRR402 pKa = 11.84 LMTISTFTVQHH413 pKa = 6.39 APVIDD418 pKa = 3.74 NLVDD422 pKa = 3.31 DD423 pKa = 5.19 FRR425 pKa = 11.84 RR426 pKa = 11.84 HH427 pKa = 3.55 ITTRR431 pKa = 11.84 IGPGDD436 pKa = 3.74 RR437 pKa = 11.84 EE438 pKa = 4.31 LACQYY443 pKa = 7.51 YY444 pKa = 6.24 TQKK447 pKa = 11.24 GEE449 pKa = 4.51 AIPEE453 pKa = 4.27 GFAMDD458 pKa = 4.88 PPAPQPGAGEE468 pKa = 4.01 ADD470 pKa = 3.2 MVQRR474 pKa = 11.84 LVEE477 pKa = 4.06 AMRR480 pKa = 11.84 GVPQQQ485 pKa = 3.11
Molecular weight: 54.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.014
IPC2_protein 5.931
IPC_protein 6.02
Toseland 6.504
ProMoST 6.351
Dawson 6.3
Bjellqvist 6.287
Wikipedia 6.313
Rodwell 6.287
Grimsley 6.693
Solomon 6.3
Lehninger 6.3
Nozaki 6.561
DTASelect 6.766
Thurlkill 6.781
EMBOSS 6.751
Sillero 6.678
Patrickios 4.253
IPC_peptide 6.313
IPC2_peptide 6.634
IPC2.peptide.svr19 6.61
Protein with the highest isoelectric point:
>tr|A0A0B5KRA3|A0A0B5KRA3_9VIRU Putative nucleoprotein OS=Tacheng Tick Virus 4 OX=1608086 GN=N PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 ALCGVGGVLILVVQSIMTAPNTTTRR27 pKa = 11.84 GSAVVTAFDD36 pKa = 4.65 CNQTTLDD43 pKa = 3.63 TVTYY47 pKa = 10.22 DD48 pKa = 3.27 LTRR51 pKa = 11.84 VAEE54 pKa = 4.52 CPDD57 pKa = 2.91 ISSRR61 pKa = 11.84 DD62 pKa = 3.31 ISEE65 pKa = 4.15 TTRR68 pKa = 11.84 LVQVIQQKK76 pKa = 9.76 EE77 pKa = 4.13 YY78 pKa = 10.64 GVLRR82 pKa = 11.84 VYY84 pKa = 10.37 RR85 pKa = 11.84 CHH87 pKa = 5.83 VTVTPQIWHH96 pKa = 6.96 CGMHH100 pKa = 5.46 SHH102 pKa = 6.54 TSIVSAPFSAQPLDD116 pKa = 3.77 VSEE119 pKa = 4.26 QEE121 pKa = 4.18 CRR123 pKa = 11.84 LMQMDD128 pKa = 4.97 GVYY131 pKa = 8.72 KK132 pKa = 9.6 TATGAIVRR140 pKa = 11.84 LKK142 pKa = 10.88 RR143 pKa = 11.84 NTTSLHH149 pKa = 5.23 TVDD152 pKa = 3.89 VAGKK156 pKa = 7.29 VHH158 pKa = 7.57 PDD160 pKa = 3.55 GTCVGGSSHH169 pKa = 7.36 INGQPYY175 pKa = 8.44 YY176 pKa = 10.61 SVVHH180 pKa = 6.39 RR181 pKa = 11.84 EE182 pKa = 3.96 AIQIRR187 pKa = 11.84 LEE189 pKa = 4.42 DD190 pKa = 3.69 KK191 pKa = 10.51 LVHH194 pKa = 5.8 YY195 pKa = 10.18 KK196 pKa = 10.23 IGDD199 pKa = 3.86 TIVVGFGGVTCRR211 pKa = 11.84 LADD214 pKa = 3.84 ISCHH218 pKa = 6.14 DD219 pKa = 3.83 FDD221 pKa = 4.48 VGRR224 pKa = 11.84 MYY226 pKa = 8.22 WTLDD230 pKa = 3.18 AAGCLDD236 pKa = 3.47 GKK238 pKa = 11.21 YY239 pKa = 10.42 DD240 pKa = 3.77 VLYY243 pKa = 10.16 TGDD246 pKa = 3.33 AAIIHH251 pKa = 5.84 TAHH254 pKa = 7.39 LGPNKK259 pKa = 10.29 EE260 pKa = 3.96 YY261 pKa = 10.26 MRR263 pKa = 11.84 VLRR266 pKa = 11.84 RR267 pKa = 11.84 ASTPFQIFTPLLGDD281 pKa = 4.14 DD282 pKa = 4.03 SACGVEE288 pKa = 5.2 VRR290 pKa = 11.84 TTPYY294 pKa = 10.49 AGLYY298 pKa = 8.09 VALYY302 pKa = 9.8 QSSPPFPIGPITYY315 pKa = 10.38 SNVDD319 pKa = 3.23 VVKK322 pKa = 10.44 YY323 pKa = 10.37 LEE325 pKa = 4.19 AKK327 pKa = 9.22 MVTFAYY333 pKa = 10.2 GSALTARR340 pKa = 11.84 EE341 pKa = 3.78 MVVNLEE347 pKa = 4.02 RR348 pKa = 11.84 QQCSLHH354 pKa = 7.19 RR355 pKa = 11.84 EE356 pKa = 3.91 TLLNRR361 pKa = 11.84 LHH363 pKa = 6.68 IVASTPSSATLLLDD377 pKa = 3.54 NEE379 pKa = 4.54 PGVFGRR385 pKa = 11.84 VAGEE389 pKa = 4.04 VVHH392 pKa = 6.86 LLRR395 pKa = 11.84 CPAVEE400 pKa = 4.15 VTLRR404 pKa = 11.84 RR405 pKa = 11.84 ADD407 pKa = 3.46 HH408 pKa = 6.94 CSLEE412 pKa = 3.8 IPIRR416 pKa = 11.84 HH417 pKa = 6.91 LGRR420 pKa = 11.84 DD421 pKa = 3.29 VYY423 pKa = 10.35 MEE425 pKa = 4.06 PRR427 pKa = 11.84 TRR429 pKa = 11.84 IITTEE434 pKa = 3.89 YY435 pKa = 8.59 THH437 pKa = 7.04 RR438 pKa = 11.84 EE439 pKa = 4.22 CGPLVPVMFRR449 pKa = 11.84 HH450 pKa = 6.18 GNQWVQFSPQYY461 pKa = 11.03 SPAPEE466 pKa = 4.1 PKK468 pKa = 9.36 KK469 pKa = 9.75 IHH471 pKa = 6.27 PMSPTLPSFEE481 pKa = 4.45 RR482 pKa = 11.84 LVAYY486 pKa = 10.12 ARR488 pKa = 11.84 GGIYY492 pKa = 10.05 DD493 pKa = 4.11 PRR495 pKa = 11.84 DD496 pKa = 3.33 LQQLRR501 pKa = 11.84 DD502 pKa = 4.71 FILFSHH508 pKa = 6.32 KK509 pKa = 9.97 RR510 pKa = 11.84 DD511 pKa = 4.01 AIEE514 pKa = 4.01 TNIVAGLSGQSAPKK528 pKa = 10.15 YY529 pKa = 10.68 DD530 pKa = 3.66 LTQVLNSGGWEE541 pKa = 3.67 EE542 pKa = 4.05 LARR545 pKa = 11.84 DD546 pKa = 4.04 RR547 pKa = 11.84 LQQIWGKK554 pKa = 9.21 FQGLGKK560 pKa = 9.91 IVNGLVGIYY569 pKa = 9.55 VCFVIVRR576 pKa = 11.84 ITFGLILRR584 pKa = 11.84 TFTIYY589 pKa = 8.91 NTHH592 pKa = 6.13 GCTKK596 pKa = 10.65 LMLGVWSSALTHH608 pKa = 6.4 FLLSLAGRR616 pKa = 11.84 EE617 pKa = 3.91 RR618 pKa = 11.84 QEE620 pKa = 3.86 PRR622 pKa = 11.84 EE623 pKa = 4.03 EE624 pKa = 4.12 SAGRR628 pKa = 11.84 YY629 pKa = 9.65 APVDD633 pKa = 3.53 QPRR636 pKa = 11.84 PPPEE640 pKa = 3.82 ATEE643 pKa = 4.66 FPSSGQGSIYY653 pKa = 10.09 PPIGLL658 pKa = 4.23
Molecular weight: 72.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.732
IPC2_protein 6.825
IPC_protein 6.795
Toseland 6.751
ProMoST 7.337
Dawson 7.366
Bjellqvist 7.585
Wikipedia 7.322
Rodwell 7.38
Grimsley 6.854
Solomon 7.395
Lehninger 7.41
Nozaki 7.863
DTASelect 7.644
Thurlkill 7.688
EMBOSS 7.673
Sillero 7.878
Patrickios 3.872
IPC_peptide 7.395
IPC2_peptide 7.395
IPC2.peptide.svr19 7.065
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3303
485
2160
1101.0
124.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.084 ± 0.922
1.605 ± 0.364
5.147 ± 0.141
5.601 ± 0.348
3.724 ± 0.252
5.298 ± 1.014
3.542 ± 0.35
6.418 ± 0.432
3.542 ± 0.443
10.173 ± 1.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.361 ± 0.436
3.149 ± 0.372
5.631 ± 0.306
4.057 ± 0.604
6.903 ± 0.051
5.359 ± 0.657
6.721 ± 0.398
6.842 ± 0.938
1.332 ± 0.138
4.511 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here