Torque teno virus 13
Average proteome isoelectric point is 7.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q99AQ6|Q99AQ6_9VIRU Orf2 OS=Torque teno virus 13 OX=687352 PE=4 SV=1
MM1 pKa = 7.49 FLGKK5 pKa = 10.46 LYY7 pKa = 10.43 RR8 pKa = 11.84 KK9 pKa = 9.31 KK10 pKa = 10.7 RR11 pKa = 11.84 KK12 pKa = 9.27 LLLQAVRR19 pKa = 11.84 APQAPSSMSSSWRR32 pKa = 11.84 VPRR35 pKa = 11.84 GDD37 pKa = 2.97 VSARR41 pKa = 11.84 EE42 pKa = 4.17 LCWYY46 pKa = 9.96 RR47 pKa = 11.84 SVRR50 pKa = 11.84 EE51 pKa = 4.01 SHH53 pKa = 7.55 DD54 pKa = 3.66 AFCGCRR60 pKa = 11.84 DD61 pKa = 3.67 PVFHH65 pKa = 7.45 LSRR68 pKa = 11.84 LAARR72 pKa = 11.84 SNHH75 pKa = 5.74 QGPPTPPTDD84 pKa = 3.69 EE85 pKa = 4.76 RR86 pKa = 11.84 PSASTPVRR94 pKa = 11.84 RR95 pKa = 11.84 LLPLPSYY102 pKa = 10.18 PGEE105 pKa = 4.49 GPQARR110 pKa = 11.84 WPGGDD115 pKa = 3.48 GEE117 pKa = 4.77 GAGDD121 pKa = 3.72 ARR123 pKa = 11.84 GGAGDD128 pKa = 3.74 GGARR132 pKa = 11.84 AGEE135 pKa = 4.12 EE136 pKa = 4.22 EE137 pKa = 4.56 YY138 pKa = 10.81 RR139 pKa = 11.84 PEE141 pKa = 5.46 DD142 pKa = 3.65 LDD144 pKa = 3.82 EE145 pKa = 5.2 LFGATEE151 pKa = 4.01 QEE153 pKa = 4.2 QQ154 pKa = 3.33
Molecular weight: 16.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.878
IPC2_protein 6.942
IPC_protein 7.263
Toseland 6.927
ProMoST 7.366
Dawson 7.805
Bjellqvist 7.761
Wikipedia 7.732
Rodwell 7.775
Grimsley 6.956
Solomon 7.98
Lehninger 7.995
Nozaki 8.097
DTASelect 7.907
Thurlkill 7.936
EMBOSS 8.068
Sillero 8.229
Patrickios 4.622
IPC_peptide 7.98
IPC2_peptide 7.556
IPC2.peptide.svr19 7.539
Protein with the highest isoelectric point:
>tr|Q99AQ6|Q99AQ6_9VIRU Orf2 OS=Torque teno virus 13 OX=687352 PE=4 SV=1
MM1 pKa = 7.07 PVIWAGMGTGGQNYY15 pKa = 9.48 AVRR18 pKa = 11.84 SDD20 pKa = 4.01 DD21 pKa = 3.71 FVVDD25 pKa = 3.65 KK26 pKa = 11.52 GFGGSFATEE35 pKa = 4.08 TFSLRR40 pKa = 11.84 VLYY43 pKa = 9.88 DD44 pKa = 2.59 QHH46 pKa = 7.53 QRR48 pKa = 11.84 GFNRR52 pKa = 11.84 WSHH55 pKa = 5.12 TNEE58 pKa = 4.02 DD59 pKa = 3.74 LDD61 pKa = 3.8 LARR64 pKa = 11.84 YY65 pKa = 9.11 RR66 pKa = 11.84 GCKK69 pKa = 4.79 WTFYY73 pKa = 10.25 RR74 pKa = 11.84 HH75 pKa = 6.93 PDD77 pKa = 2.97 TDD79 pKa = 4.08 FIVYY83 pKa = 7.46 FTNNPPMKK91 pKa = 8.68 TNQYY95 pKa = 7.19 TAPLTTPGMLMRR107 pKa = 11.84 SKK109 pKa = 10.99 YY110 pKa = 10.26 KK111 pKa = 10.39 ILIPSFKK118 pKa = 10.0 TKK120 pKa = 10.41 PKK122 pKa = 9.67 GKK124 pKa = 8.58 KK125 pKa = 8.16 TISFRR130 pKa = 11.84 ARR132 pKa = 11.84 PPKK135 pKa = 10.3 LFQDD139 pKa = 3.03 KK140 pKa = 9.91 WYY142 pKa = 8.12 TQQDD146 pKa = 4.26 LCPVPLIQLNLTAADD161 pKa = 3.93 FTHH164 pKa = 7.36 PFGLPLTDD172 pKa = 3.72 SPCVRR177 pKa = 11.84 FQVLGDD183 pKa = 4.26 LYY185 pKa = 11.29 NNCLNIDD192 pKa = 4.43 LPQFDD197 pKa = 5.0 DD198 pKa = 4.48 KK199 pKa = 11.54 GTISDD204 pKa = 3.53 ASSYY208 pKa = 11.45 SRR210 pKa = 11.84 DD211 pKa = 3.06 NKK213 pKa = 9.92 QQLEE217 pKa = 4.28 EE218 pKa = 4.28 LYY220 pKa = 9.13 KK221 pKa = 10.4 TLFVKK226 pKa = 10.49 KK227 pKa = 10.38 GCGHH231 pKa = 6.32 YY232 pKa = 9.46 WQTFMTNSMVKK243 pKa = 10.08 AHH245 pKa = 7.05 IDD247 pKa = 3.0 AAQAQNHH254 pKa = 4.92 QQDD257 pKa = 3.43 TSGPQSAKK265 pKa = 10.94 DD266 pKa = 3.69 PFPTKK271 pKa = 9.64 PDD273 pKa = 3.49 RR274 pKa = 11.84 NQFEE278 pKa = 3.85 QWKK281 pKa = 10.5 NKK283 pKa = 8.23 FTDD286 pKa = 3.56 PRR288 pKa = 11.84 DD289 pKa = 3.91 SNFLFATYY297 pKa = 9.82 HH298 pKa = 6.63 PEE300 pKa = 5.06 NITQTIKK307 pKa = 10.13 TMRR310 pKa = 11.84 DD311 pKa = 2.8 NNFALEE317 pKa = 4.1 TGKK320 pKa = 10.31 NDD322 pKa = 4.71 LYY324 pKa = 11.55 GDD326 pKa = 3.64 YY327 pKa = 10.19 QAQYY331 pKa = 9.38 TRR333 pKa = 11.84 NTHH336 pKa = 6.52 LLDD339 pKa = 3.92 YY340 pKa = 10.74 YY341 pKa = 11.08 LGFYY345 pKa = 10.79 SPIFLSSGRR354 pKa = 11.84 SNTEE358 pKa = 3.05 FFTAYY363 pKa = 10.31 RR364 pKa = 11.84 DD365 pKa = 3.62 IIYY368 pKa = 10.85 NPLLDD373 pKa = 4.44 KK374 pKa = 10.82 GTGNMIWFQYY384 pKa = 7.77 HH385 pKa = 5.84 TKK387 pKa = 9.48 TDD389 pKa = 3.86 NIFKK393 pKa = 10.47 KK394 pKa = 10.22 PEE396 pKa = 4.1 CHH398 pKa = 6.57 WEE400 pKa = 3.99 ILDD403 pKa = 3.64 MPLWALCNGYY413 pKa = 9.82 KK414 pKa = 10.14 EE415 pKa = 4.23 YY416 pKa = 11.25 LEE418 pKa = 4.45 SQIKK422 pKa = 10.37 YY423 pKa = 10.75 GDD425 pKa = 3.41 ILVEE429 pKa = 4.09 GKK431 pKa = 10.24 VLIRR435 pKa = 11.84 CPYY438 pKa = 8.16 TKK440 pKa = 10.34 PPLADD445 pKa = 4.19 PNNSLAGYY453 pKa = 8.99 VVYY456 pKa = 9.02 NTNFGQGKK464 pKa = 8.47 WIDD467 pKa = 3.59 GKK469 pKa = 11.15 GYY471 pKa = 9.7 IPLRR475 pKa = 11.84 HH476 pKa = 5.78 RR477 pKa = 11.84 SKK479 pKa = 9.84 WYY481 pKa = 10.47 VMLMYY486 pKa = 9.71 QTDD489 pKa = 3.79 VLHH492 pKa = 7.41 DD493 pKa = 4.63 LVTCGPWQYY502 pKa = 11.46 RR503 pKa = 11.84 DD504 pKa = 3.79 DD505 pKa = 4.26 NKK507 pKa = 10.86 NSQLIAKK514 pKa = 9.29 YY515 pKa = 10.25 RR516 pKa = 11.84 FTFYY520 pKa = 10.02 WGGNMVHH527 pKa = 6.07 SQVIRR532 pKa = 11.84 NPCKK536 pKa = 9.04 DD537 pKa = 3.59 TQVSGPRR544 pKa = 11.84 RR545 pKa = 11.84 QPRR548 pKa = 11.84 EE549 pKa = 3.74 IQVVDD554 pKa = 3.98 PQLITPPWVLHH565 pKa = 6.59 SFDD568 pKa = 3.34 QRR570 pKa = 11.84 RR571 pKa = 11.84 GMFTEE576 pKa = 4.12 TAIRR580 pKa = 11.84 RR581 pKa = 11.84 LLRR584 pKa = 11.84 QPLPGEE590 pKa = 4.13 YY591 pKa = 10.04 APPALRR597 pKa = 11.84 VPLLFPSSEE606 pKa = 3.95 FQRR609 pKa = 11.84 EE610 pKa = 4.41 GEE612 pKa = 4.23 GAEE615 pKa = 4.45 SDD617 pKa = 3.59 LSSPAKK623 pKa = 10.17 RR624 pKa = 11.84 PRR626 pKa = 11.84 LWQEE630 pKa = 3.72 EE631 pKa = 4.08 DD632 pKa = 4.7 SEE634 pKa = 4.73 TQTQSSEE641 pKa = 4.2 GPAEE645 pKa = 4.14 TTRR648 pKa = 11.84 EE649 pKa = 3.86 LLEE652 pKa = 4.27 RR653 pKa = 11.84 KK654 pKa = 9.48 LRR656 pKa = 11.84 EE657 pKa = 3.84 QRR659 pKa = 11.84 VLNLQLQQFAVQLAKK674 pKa = 9.61 TQANLHH680 pKa = 6.26 INPLLYY686 pKa = 10.55 SQQQ689 pKa = 3.22
Molecular weight: 80.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.736
IPC2_protein 7.995
IPC_protein 7.849
Toseland 7.761
ProMoST 8.361
Dawson 8.609
Bjellqvist 8.77
Wikipedia 8.624
Rodwell 8.653
Grimsley 7.819
Solomon 8.668
Lehninger 8.653
Nozaki 8.829
DTASelect 8.58
Thurlkill 8.639
EMBOSS 8.77
Sillero 8.887
Patrickios 4.19
IPC_peptide 8.668
IPC2_peptide 7.6
IPC2.peptide.svr19 7.846
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
843
154
689
421.5
48.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.457 ± 2.025
1.542 ± 0.192
6.287 ± 0.209
4.745 ± 1.44
4.864 ± 1.071
6.88 ± 2.273
2.254 ± 0.145
3.321 ± 1.57
5.338 ± 1.295
9.015 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.898 ± 0.283
4.508 ± 1.824
7.592 ± 1.322
6.406 ± 1.186
6.88 ± 2.273
5.813 ± 1.243
6.406 ± 1.8
3.915 ± 0.009
2.135 ± 0.088
4.745 ± 1.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here