Bacillus phage Kamfam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bastillevirus; unclassified Bastillevirus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 291 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385E5I5|A0A385E5I5_9CAUD Uncharacterized protein OS=Bacillus phage Kamfam OX=2301684 GN=284 PE=4 SV=1
MM1 pKa = 7.14TKK3 pKa = 10.36KK4 pKa = 10.85LEE6 pKa = 4.12LAQYY10 pKa = 10.85DD11 pKa = 4.36YY12 pKa = 11.57IEE14 pKa = 4.18VLEE17 pKa = 4.65DD18 pKa = 4.15EE19 pKa = 4.6IDD21 pKa = 3.51VLEE24 pKa = 4.96RR25 pKa = 11.84FNEE28 pKa = 4.23YY29 pKa = 10.54DD30 pKa = 3.66GSTHH34 pKa = 6.12ICDD37 pKa = 5.5AITEE41 pKa = 4.22IADD44 pKa = 3.67AAVSIYY50 pKa = 10.3PADD53 pKa = 3.45LWKK56 pKa = 10.58NAYY59 pKa = 8.22EE60 pKa = 3.83MKK62 pKa = 10.35EE63 pKa = 4.08YY64 pKa = 10.29IEE66 pKa = 4.33EE67 pKa = 4.19AMSNGLCEE75 pKa = 4.43TPHH78 pKa = 6.04GQQPDD83 pKa = 3.46LDD85 pKa = 4.57KK86 pKa = 11.21IFAAGYY92 pKa = 8.43YY93 pKa = 9.71EE94 pKa = 5.04YY95 pKa = 11.19YY96 pKa = 10.58SQLLYY101 pKa = 11.19NNEE104 pKa = 3.58SEE106 pKa = 4.23MYY108 pKa = 9.68YY109 pKa = 10.82NYY111 pKa = 9.98IANIVNKK118 pKa = 9.69WIEE121 pKa = 4.05TLNYY125 pKa = 8.73EE126 pKa = 4.36QLEE129 pKa = 4.17KK130 pKa = 11.04LDD132 pKa = 4.12IDD134 pKa = 4.03NLDD137 pKa = 3.3EE138 pKa = 5.26RR139 pKa = 11.84IEE141 pKa = 4.05YY142 pKa = 10.31EE143 pKa = 5.71KK144 pKa = 11.44GDD146 pKa = 3.74IDD148 pKa = 3.92NNSYY152 pKa = 9.98MEE154 pKa = 4.63DD155 pKa = 3.8LEE157 pKa = 6.25DD158 pKa = 3.21IAKK161 pKa = 10.47RR162 pKa = 11.84IIAEE166 pKa = 4.2FKK168 pKa = 11.13GKK170 pKa = 9.73VNKK173 pKa = 9.88NDD175 pKa = 2.66AWEE178 pKa = 3.93NHH180 pKa = 5.76GDD182 pKa = 4.36VDD184 pKa = 5.23ALDD187 pKa = 3.3QGGIFIRR194 pKa = 11.84PDD196 pKa = 2.83EE197 pKa = 4.37EE198 pKa = 4.14QRR200 pKa = 11.84HH201 pKa = 5.9CYY203 pKa = 9.94YY204 pKa = 10.52VVKK207 pKa = 10.51VDD209 pKa = 3.38NLEE212 pKa = 4.14YY213 pKa = 10.0VTNEE217 pKa = 3.49DD218 pKa = 4.25KK219 pKa = 11.04IRR221 pKa = 11.84ITDD224 pKa = 3.65MYY226 pKa = 11.47VDD228 pKa = 5.4ISDD231 pKa = 5.52DD232 pKa = 3.26WFNWAGIEE240 pKa = 4.23AYY242 pKa = 10.45SGTEE246 pKa = 3.74EE247 pKa = 4.96SDD249 pKa = 3.62DD250 pKa = 5.27DD251 pKa = 4.0IEE253 pKa = 4.95KK254 pKa = 10.53VLAVIDD260 pKa = 3.47YY261 pKa = 10.76HH262 pKa = 7.24GAEE265 pKa = 4.58NFNPDD270 pKa = 3.76IEE272 pKa = 4.7DD273 pKa = 4.2FEE275 pKa = 5.75DD276 pKa = 3.59KK277 pKa = 11.12ADD279 pKa = 5.02AIDD282 pKa = 4.09FLADD286 pKa = 3.03KK287 pKa = 10.57GIAIEE292 pKa = 4.28EE293 pKa = 4.36VV294 pKa = 3.21

Molecular weight: 34.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385E602|A0A385E602_9CAUD Holliday junction resolvase OS=Bacillus phage Kamfam OX=2301684 GN=135 PE=4 SV=1
MM1 pKa = 7.44AKK3 pKa = 9.74KK4 pKa = 10.02RR5 pKa = 11.84PKK7 pKa = 10.07IFQSEE12 pKa = 4.23ANMKK16 pKa = 10.32KK17 pKa = 10.72AMNNLGNTLVNNALDD32 pKa = 3.81EE33 pKa = 4.46TAKK36 pKa = 10.84SLARR40 pKa = 11.84NLPDD44 pKa = 3.81GMEE47 pKa = 4.8LKK49 pKa = 10.56RR50 pKa = 11.84KK51 pKa = 9.02PKK53 pKa = 10.18YY54 pKa = 10.51LEE56 pKa = 3.82LTEE59 pKa = 4.68NRR61 pKa = 11.84LNSLGVIDD69 pKa = 5.17LKK71 pKa = 11.17PYY73 pKa = 9.87FARR76 pKa = 11.84SSKK79 pKa = 10.28RR80 pKa = 11.84KK81 pKa = 8.3NRR83 pKa = 11.84KK84 pKa = 8.52GGGWYY89 pKa = 8.56LTVPIRR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84ARR99 pKa = 11.84GMSRR103 pKa = 11.84RR104 pKa = 11.84MYY106 pKa = 9.26EE107 pKa = 3.53QLRR110 pKa = 11.84AIDD113 pKa = 3.65IAPSQRR119 pKa = 11.84KK120 pKa = 6.25TVISDD125 pKa = 2.95YY126 pKa = 10.63MYY128 pKa = 11.18DD129 pKa = 3.39NRR131 pKa = 11.84KK132 pKa = 7.74TSDD135 pKa = 3.12ATMLNYY141 pKa = 9.76TPRR144 pKa = 11.84SNNITKK150 pKa = 9.76IRR152 pKa = 11.84SGTNRR157 pKa = 11.84HH158 pKa = 6.43DD159 pKa = 4.16YY160 pKa = 10.48IAFRR164 pKa = 11.84TVSDD168 pKa = 3.95KK169 pKa = 11.56SPANSWIINRR179 pKa = 11.84DD180 pKa = 3.06RR181 pKa = 11.84VTVSNTSKK189 pKa = 9.82TFVANVNRR197 pKa = 11.84LMKK200 pKa = 10.92YY201 pKa = 9.54NMKK204 pKa = 10.23NQQ206 pKa = 3.17

Molecular weight: 23.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

291

0

291

49456

21

2031

170.0

19.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.007 ± 0.2

0.865 ± 0.062

6.671 ± 0.139

8.244 ± 0.286

4.03 ± 0.101

6.173 ± 0.246

1.893 ± 0.11

6.727 ± 0.164

7.857 ± 0.194

8.256 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.952 ± 0.096

5.443 ± 0.154

2.819 ± 0.127

3.264 ± 0.121

4.386 ± 0.133

5.583 ± 0.15

5.955 ± 0.201

7.071 ± 0.15

1.189 ± 0.059

4.616 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski