Amino acid dipepetide frequency for Bacillus phage Kamfam

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.145AlaAla: 4.145 ± 0.448
0.485AlaCys: 0.485 ± 0.086
3.458AlaAsp: 3.458 ± 0.248
3.66AlaGlu: 3.66 ± 0.253
2.649AlaPhe: 2.649 ± 0.22
3.721AlaGly: 3.721 ± 0.379
1.173AlaHis: 1.173 ± 0.143
3.68AlaIle: 3.68 ± 0.281
4.833AlaLys: 4.833 ± 0.226
5.419AlaLeu: 5.419 ± 0.304
1.8AlaMet: 1.8 ± 0.23
3.235AlaAsn: 3.235 ± 0.292
2.325AlaPro: 2.325 ± 0.241
2.325AlaGln: 2.325 ± 0.203
2.892AlaArg: 2.892 ± 0.278
3.518AlaSer: 3.518 ± 0.29
3.599AlaThr: 3.599 ± 0.33
3.68AlaVal: 3.68 ± 0.276
0.708AlaTrp: 0.708 ± 0.112
2.77AlaTyr: 2.77 ± 0.209
0.0AlaXaa: 0.0 ± 0.0
Cys
0.324CysAla: 0.324 ± 0.069
0.121CysCys: 0.121 ± 0.046
0.89CysAsp: 0.89 ± 0.148
0.485CysGlu: 0.485 ± 0.088
0.283CysPhe: 0.283 ± 0.092
0.586CysGly: 0.586 ± 0.123
0.243CysHis: 0.243 ± 0.066
0.647CysIle: 0.647 ± 0.131
0.708CysLys: 0.708 ± 0.136
0.566CysLeu: 0.566 ± 0.107
0.344CysMet: 0.344 ± 0.08
0.384CysAsn: 0.384 ± 0.095
0.324CysPro: 0.324 ± 0.096
0.162CysGln: 0.162 ± 0.062
0.222CysArg: 0.222 ± 0.06
0.506CysSer: 0.506 ± 0.103
0.586CysThr: 0.586 ± 0.097
0.627CysVal: 0.627 ± 0.114
0.121CysTrp: 0.121 ± 0.05
0.526CysTyr: 0.526 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
4.064AspAla: 4.064 ± 0.28
0.586AspCys: 0.586 ± 0.11
4.064AspAsp: 4.064 ± 0.335
6.005AspGlu: 6.005 ± 0.42
3.114AspPhe: 3.114 ± 0.211
4.125AspGly: 4.125 ± 0.335
0.647AspHis: 0.647 ± 0.107
5.46AspIle: 5.46 ± 0.348
6.107AspLys: 6.107 ± 0.387
5.055AspLeu: 5.055 ± 0.3
2.022AspMet: 2.022 ± 0.227
3.842AspAsn: 3.842 ± 0.285
1.395AspPro: 1.395 ± 0.173
0.971AspGln: 0.971 ± 0.129
3.033AspArg: 3.033 ± 0.237
3.377AspSer: 3.377 ± 0.313
4.085AspThr: 4.085 ± 0.368
4.489AspVal: 4.489 ± 0.347
0.971AspTrp: 0.971 ± 0.148
3.276AspTyr: 3.276 ± 0.239
0.0AspXaa: 0.0 ± 0.0
Glu
4.954GluAla: 4.954 ± 0.364
0.667GluCys: 0.667 ± 0.121
5.702GluAsp: 5.702 ± 0.451
8.594GluGlu: 8.594 ± 0.733
3.478GluPhe: 3.478 ± 0.27
4.61GluGly: 4.61 ± 0.259
1.638GluHis: 1.638 ± 0.173
5.581GluIle: 5.581 ± 0.387
6.046GluLys: 6.046 ± 0.446
8.331GluLeu: 8.331 ± 0.612
2.993GluMet: 2.993 ± 0.26
3.296GluAsn: 3.296 ± 0.245
1.779GluPro: 1.779 ± 0.195
3.235GluGln: 3.235 ± 0.252
3.579GluArg: 3.579 ± 0.312
3.923GluSer: 3.923 ± 0.291
3.417GluThr: 3.417 ± 0.235
6.046GluVal: 6.046 ± 0.424
1.153GluTrp: 1.153 ± 0.162
3.417GluTyr: 3.417 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.447PheAla: 2.447 ± 0.207
0.546PheCys: 0.546 ± 0.103
3.154PheAsp: 3.154 ± 0.231
2.851PheGlu: 2.851 ± 0.218
1.233PhePhe: 1.233 ± 0.179
1.901PheGly: 1.901 ± 0.164
0.93PheHis: 0.93 ± 0.139
2.629PheIle: 2.629 ± 0.23
3.094PheLys: 3.094 ± 0.273
3.357PheLeu: 3.357 ± 0.264
1.092PheMet: 1.092 ± 0.128
2.588PheAsn: 2.588 ± 0.207
1.031PhePro: 1.031 ± 0.138
1.051PheGln: 1.051 ± 0.144
1.557PheArg: 1.557 ± 0.16
2.507PheSer: 2.507 ± 0.231
3.094PheThr: 3.094 ± 0.242
3.134PheVal: 3.134 ± 0.241
0.384PheTrp: 0.384 ± 0.088
1.719PheTyr: 1.719 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
3.68GlyAla: 3.68 ± 0.472
0.546GlyCys: 0.546 ± 0.11
4.165GlyAsp: 4.165 ± 0.315
4.63GlyGlu: 4.63 ± 0.285
2.993GlyPhe: 2.993 ± 0.255
5.015GlyGly: 5.015 ± 0.627
0.95GlyHis: 0.95 ± 0.152
4.368GlyIle: 4.368 ± 0.334
4.732GlyLys: 4.732 ± 0.311
4.165GlyLeu: 4.165 ± 0.357
2.143GlyMet: 2.143 ± 0.218
3.316GlyAsn: 3.316 ± 0.315
0.0GlyPro: 0.0 ± 0.0
1.88GlyGln: 1.88 ± 0.185
2.689GlyArg: 2.689 ± 0.228
3.498GlySer: 3.498 ± 0.367
3.822GlyThr: 3.822 ± 0.3
4.954GlyVal: 4.954 ± 0.323
0.95GlyTrp: 0.95 ± 0.126
3.235GlyTyr: 3.235 ± 0.235
0.0GlyXaa: 0.0 ± 0.0
His
0.93HisAla: 0.93 ± 0.128
0.182HisCys: 0.182 ± 0.067
1.132HisAsp: 1.132 ± 0.154
1.557HisGlu: 1.557 ± 0.18
0.89HisPhe: 0.89 ± 0.161
1.051HisGly: 1.051 ± 0.128
0.404HisHis: 0.404 ± 0.1
1.517HisIle: 1.517 ± 0.169
1.537HisLys: 1.537 ± 0.18
1.213HisLeu: 1.213 ± 0.162
0.647HisMet: 0.647 ± 0.146
1.294HisAsn: 1.294 ± 0.176
0.708HisPro: 0.708 ± 0.121
0.506HisGln: 0.506 ± 0.1
0.708HisArg: 0.708 ± 0.116
0.991HisSer: 0.991 ± 0.147
1.051HisThr: 1.051 ± 0.161
1.375HisVal: 1.375 ± 0.172
0.243HisTrp: 0.243 ± 0.066
0.991HisTyr: 0.991 ± 0.14
0.0HisXaa: 0.0 ± 0.0
Ile
4.226IleAla: 4.226 ± 0.291
0.546IleCys: 0.546 ± 0.116
5.156IleAsp: 5.156 ± 0.278
5.844IleGlu: 5.844 ± 0.413
1.921IlePhe: 1.921 ± 0.2
4.044IleGly: 4.044 ± 0.339
1.132IleHis: 1.132 ± 0.155
4.489IleIle: 4.489 ± 0.375
6.208IleLys: 6.208 ± 0.338
4.833IleLeu: 4.833 ± 0.349
1.86IleMet: 1.86 ± 0.197
3.619IleAsn: 3.619 ± 0.292
2.265IlePro: 2.265 ± 0.206
2.123IleGln: 2.123 ± 0.188
3.175IleArg: 3.175 ± 0.23
3.862IleSer: 3.862 ± 0.234
4.347IleThr: 4.347 ± 0.317
4.59IleVal: 4.59 ± 0.295
0.404IleTrp: 0.404 ± 0.085
2.629IleTyr: 2.629 ± 0.208
0.0IleXaa: 0.0 ± 0.0
Lys
4.651LysAla: 4.651 ± 0.263
0.546LysCys: 0.546 ± 0.119
5.561LysAsp: 5.561 ± 0.329
8.351LysGlu: 8.351 ± 0.466
2.528LysPhe: 2.528 ± 0.205
5.52LysGly: 5.52 ± 0.31
1.517LysHis: 1.517 ± 0.193
4.691LysIle: 4.691 ± 0.295
5.945LysLys: 5.945 ± 0.429
6.551LysLeu: 6.551 ± 0.438
2.588LysMet: 2.588 ± 0.247
3.761LysAsn: 3.761 ± 0.306
2.164LysPro: 2.164 ± 0.229
2.811LysGln: 2.811 ± 0.264
3.741LysArg: 3.741 ± 0.31
3.66LysSer: 3.66 ± 0.327
3.68LysThr: 3.68 ± 0.278
5.965LysVal: 5.965 ± 0.379
0.91LysTrp: 0.91 ± 0.141
3.437LysTyr: 3.437 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
5.379LeuAla: 5.379 ± 0.334
0.829LeuCys: 0.829 ± 0.124
6.187LeuAsp: 6.187 ± 0.356
7.623LeuGlu: 7.623 ± 0.466
3.417LeuPhe: 3.417 ± 0.269
5.116LeuGly: 5.116 ± 0.31
1.84LeuHis: 1.84 ± 0.186
4.833LeuIle: 4.833 ± 0.357
6.551LeuLys: 6.551 ± 0.386
6.309LeuLeu: 6.309 ± 0.409
2.204LeuMet: 2.204 ± 0.236
3.842LeuAsn: 3.842 ± 0.272
2.71LeuPro: 2.71 ± 0.227
2.932LeuGln: 2.932 ± 0.238
3.579LeuArg: 3.579 ± 0.28
4.974LeuSer: 4.974 ± 0.389
5.136LeuThr: 5.136 ± 0.291
5.682LeuVal: 5.682 ± 0.368
0.708LeuTrp: 0.708 ± 0.117
2.71LeuTyr: 2.71 ± 0.232
0.0LeuXaa: 0.0 ± 0.0
Met
2.123MetAla: 2.123 ± 0.192
0.263MetCys: 0.263 ± 0.078
1.577MetAsp: 1.577 ± 0.204
2.265MetGlu: 2.265 ± 0.227
1.092MetPhe: 1.092 ± 0.147
1.537MetGly: 1.537 ± 0.223
0.546MetHis: 0.546 ± 0.113
2.164MetIle: 2.164 ± 0.218
2.507MetLys: 2.507 ± 0.229
2.548MetLeu: 2.548 ± 0.263
0.566MetMet: 0.566 ± 0.119
1.779MetAsn: 1.779 ± 0.192
0.748MetPro: 0.748 ± 0.121
0.91MetGln: 0.91 ± 0.123
1.476MetArg: 1.476 ± 0.169
2.164MetSer: 2.164 ± 0.214
1.88MetThr: 1.88 ± 0.247
1.719MetVal: 1.719 ± 0.172
0.445MetTrp: 0.445 ± 0.121
1.213MetTyr: 1.213 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
2.811AsnAla: 2.811 ± 0.275
0.404AsnCys: 0.404 ± 0.096
3.195AsnAsp: 3.195 ± 0.232
3.397AsnGlu: 3.397 ± 0.23
2.062AsnPhe: 2.062 ± 0.2
4.368AsnGly: 4.368 ± 0.375
1.193AsnHis: 1.193 ± 0.132
3.397AsnIle: 3.397 ± 0.299
4.267AsnLys: 4.267 ± 0.259
4.489AsnLeu: 4.489 ± 0.278
1.84AsnMet: 1.84 ± 0.199
3.377AsnAsn: 3.377 ± 0.335
2.285AsnPro: 2.285 ± 0.246
1.658AsnGln: 1.658 ± 0.214
2.669AsnArg: 2.669 ± 0.256
2.851AsnSer: 2.851 ± 0.263
3.094AsnThr: 3.094 ± 0.3
3.64AsnVal: 3.64 ± 0.256
0.647AsnTrp: 0.647 ± 0.122
2.79AsnTyr: 2.79 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
1.739ProAla: 1.739 ± 0.188
0.142ProCys: 0.142 ± 0.056
2.305ProAsp: 2.305 ± 0.237
2.507ProGlu: 2.507 ± 0.298
1.132ProPhe: 1.132 ± 0.182
0.04ProGly: 0.04 ± 0.027
0.627ProHis: 0.627 ± 0.106
1.82ProIle: 1.82 ± 0.191
2.285ProLys: 2.285 ± 0.227
2.426ProLeu: 2.426 ± 0.24
0.809ProMet: 0.809 ± 0.145
2.083ProAsn: 2.083 ± 0.23
0.728ProPro: 0.728 ± 0.1
0.89ProGln: 0.89 ± 0.145
1.233ProArg: 1.233 ± 0.154
1.961ProSer: 1.961 ± 0.232
1.982ProThr: 1.982 ± 0.243
1.82ProVal: 1.82 ± 0.205
0.263ProTrp: 0.263 ± 0.075
1.395ProTyr: 1.395 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
2.062GlnAla: 2.062 ± 0.244
0.182GlnCys: 0.182 ± 0.056
1.84GlnAsp: 1.84 ± 0.164
2.689GlnGlu: 2.689 ± 0.237
1.112GlnPhe: 1.112 ± 0.16
2.042GlnGly: 2.042 ± 0.26
0.586GlnHis: 0.586 ± 0.107
1.86GlnIle: 1.86 ± 0.225
2.204GlnLys: 2.204 ± 0.175
2.79GlnLeu: 2.79 ± 0.286
1.112GlnMet: 1.112 ± 0.142
1.719GlnAsn: 1.719 ± 0.219
1.335GlnPro: 1.335 ± 0.207
1.739GlnGln: 1.739 ± 0.39
1.456GlnArg: 1.456 ± 0.202
1.84GlnSer: 1.84 ± 0.244
1.88GlnThr: 1.88 ± 0.184
2.224GlnVal: 2.224 ± 0.198
0.425GlnTrp: 0.425 ± 0.099
1.537GlnTyr: 1.537 ± 0.159
0.0GlnXaa: 0.0 ± 0.0
Arg
2.608ArgAla: 2.608 ± 0.214
0.465ArgCys: 0.465 ± 0.089
3.094ArgAsp: 3.094 ± 0.283
3.579ArgGlu: 3.579 ± 0.363
2.022ArgPhe: 2.022 ± 0.2
2.932ArgGly: 2.932 ± 0.242
0.708ArgHis: 0.708 ± 0.128
3.195ArgIle: 3.195 ± 0.259
3.761ArgLys: 3.761 ± 0.331
4.226ArgLeu: 4.226 ± 0.283
1.213ArgMet: 1.213 ± 0.153
2.265ArgAsn: 2.265 ± 0.233
0.89ArgPro: 0.89 ± 0.137
1.415ArgGln: 1.415 ± 0.188
1.8ArgArg: 1.8 ± 0.175
1.86ArgSer: 1.86 ± 0.22
2.406ArgThr: 2.406 ± 0.239
2.952ArgVal: 2.952 ± 0.223
0.445ArgTrp: 0.445 ± 0.099
2.022ArgTyr: 2.022 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
3.255SerAla: 3.255 ± 0.293
0.283SerCys: 0.283 ± 0.069
2.952SerAsp: 2.952 ± 0.254
3.983SerGlu: 3.983 ± 0.37
2.71SerPhe: 2.71 ± 0.209
4.044SerGly: 4.044 ± 0.288
1.092SerHis: 1.092 ± 0.196
4.408SerIle: 4.408 ± 0.28
3.923SerLys: 3.923 ± 0.301
4.812SerLeu: 4.812 ± 0.29
1.699SerMet: 1.699 ± 0.171
2.75SerAsn: 2.75 ± 0.303
1.678SerPro: 1.678 ± 0.194
1.779SerGln: 1.779 ± 0.209
2.143SerArg: 2.143 ± 0.197
3.195SerSer: 3.195 ± 0.308
3.377SerThr: 3.377 ± 0.287
3.963SerVal: 3.963 ± 0.247
0.971SerTrp: 0.971 ± 0.151
2.811SerTyr: 2.811 ± 0.235
0.0SerXaa: 0.0 ± 0.0
Thr
3.458ThrAla: 3.458 ± 0.373
0.546ThrCys: 0.546 ± 0.11
3.559ThrAsp: 3.559 ± 0.288
3.7ThrGlu: 3.7 ± 0.301
2.467ThrPhe: 2.467 ± 0.258
3.983ThrGly: 3.983 ± 0.332
0.971ThrHis: 0.971 ± 0.156
4.145ThrIle: 4.145 ± 0.302
4.165ThrLys: 4.165 ± 0.314
5.197ThrLeu: 5.197 ± 0.289
1.233ThrMet: 1.233 ± 0.159
3.255ThrAsn: 3.255 ± 0.282
2.265ThrPro: 2.265 ± 0.233
1.759ThrGln: 1.759 ± 0.209
2.447ThrArg: 2.447 ± 0.217
3.579ThrSer: 3.579 ± 0.317
3.801ThrThr: 3.801 ± 0.316
4.893ThrVal: 4.893 ± 0.35
0.708ThrTrp: 0.708 ± 0.129
3.417ThrTyr: 3.417 ± 0.263
0.0ThrXaa: 0.0 ± 0.0
Val
4.287ValAla: 4.287 ± 0.288
0.647ValCys: 0.647 ± 0.133
4.873ValAsp: 4.873 ± 0.22
5.702ValGlu: 5.702 ± 0.328
3.033ValPhe: 3.033 ± 0.242
4.064ValGly: 4.064 ± 0.297
1.456ValHis: 1.456 ± 0.18
4.226ValIle: 4.226 ± 0.312
5.581ValLys: 5.581 ± 0.334
5.682ValLeu: 5.682 ± 0.36
1.699ValMet: 1.699 ± 0.171
3.842ValAsn: 3.842 ± 0.296
2.042ValPro: 2.042 ± 0.216
2.386ValGln: 2.386 ± 0.221
2.669ValArg: 2.669 ± 0.238
4.024ValSer: 4.024 ± 0.257
4.893ValThr: 4.893 ± 0.304
5.237ValVal: 5.237 ± 0.347
0.687ValTrp: 0.687 ± 0.118
3.68ValTyr: 3.68 ± 0.291
0.0ValXaa: 0.0 ± 0.0
Trp
0.667TrpAla: 0.667 ± 0.117
0.162TrpCys: 0.162 ± 0.05
0.869TrpAsp: 0.869 ± 0.131
1.193TrpGlu: 1.193 ± 0.141
0.627TrpPhe: 0.627 ± 0.138
0.485TrpGly: 0.485 ± 0.088
0.344TrpHis: 0.344 ± 0.085
0.829TrpIle: 0.829 ± 0.142
0.546TrpLys: 0.546 ± 0.107
1.112TrpLeu: 1.112 ± 0.163
0.222TrpMet: 0.222 ± 0.062
0.809TrpAsn: 0.809 ± 0.141
0.0TrpPro: 0.0 ± 0.0
0.425TrpGln: 0.425 ± 0.088
0.586TrpArg: 0.586 ± 0.109
0.89TrpSer: 0.89 ± 0.146
0.627TrpThr: 0.627 ± 0.108
0.789TrpVal: 0.789 ± 0.119
0.182TrpTrp: 0.182 ± 0.055
0.526TrpTyr: 0.526 ± 0.088
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.265TyrAla: 2.265 ± 0.216
0.506TyrCys: 0.506 ± 0.112
2.972TyrAsp: 2.972 ± 0.222
3.822TyrGlu: 3.822 ± 0.316
1.537TyrPhe: 1.537 ± 0.161
2.548TyrGly: 2.548 ± 0.254
0.93TyrHis: 0.93 ± 0.125
3.336TyrIle: 3.336 ± 0.283
3.579TyrLys: 3.579 ± 0.281
3.478TyrLeu: 3.478 ± 0.298
1.415TyrMet: 1.415 ± 0.15
3.377TyrAsn: 3.377 ± 0.26
1.517TyrPro: 1.517 ± 0.153
1.678TyrGln: 1.678 ± 0.179
2.204TyrArg: 2.204 ± 0.193
2.649TyrSer: 2.649 ± 0.244
2.79TyrThr: 2.79 ± 0.239
2.932TyrVal: 2.932 ± 0.225
0.566TyrTrp: 0.566 ± 0.094
2.062TyrTyr: 2.062 ± 0.247
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 291 proteins (49456 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski