Granulicatella sp. zg-ZJ

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Granulicatella; unclassified Granulicatella

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0L5T9|A0A6M0L5T9_9LACT UPF0342 protein GMA11_05885 OS=Granulicatella sp. zg-ZJ OX=2678504 GN=GMA11_05885 PE=3 SV=1
MM1 pKa = 7.16VSALVVFASLTGSTEE16 pKa = 3.57EE17 pKa = 4.46CADD20 pKa = 3.22IVAEE24 pKa = 4.07SLEE27 pKa = 4.05ALGAQVDD34 pKa = 4.39VVEE37 pKa = 5.07CSQASASDD45 pKa = 3.54FQKK48 pKa = 11.19YY49 pKa = 9.61DD50 pKa = 3.17LCLVGSYY57 pKa = 9.91TYY59 pKa = 11.43GDD61 pKa = 3.76GEE63 pKa = 4.5LPDD66 pKa = 5.09EE67 pKa = 4.52IVDD70 pKa = 4.38LYY72 pKa = 11.48EE73 pKa = 4.15EE74 pKa = 4.3LAEE77 pKa = 4.46EE78 pKa = 4.37DD79 pKa = 3.41LSGKK83 pKa = 9.84VFGVFGSGDD92 pKa = 3.41TYY94 pKa = 11.32YY95 pKa = 11.26DD96 pKa = 3.89HH97 pKa = 7.16YY98 pKa = 9.73CQAVILFEE106 pKa = 4.14EE107 pKa = 4.65QFGKK111 pKa = 9.05TGALKK116 pKa = 10.44GAEE119 pKa = 4.12SVKK122 pKa = 10.35IEE124 pKa = 4.28LNAEE128 pKa = 3.66EE129 pKa = 5.64DD130 pKa = 4.63DD131 pKa = 4.92IEE133 pKa = 4.2NLKK136 pKa = 10.5NLSKK140 pKa = 10.85QLITSFEE147 pKa = 4.08NKK149 pKa = 9.72

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0L9H6|A0A6M0L9H6_9LACT Methionine import ATP-binding protein MetN OS=Granulicatella sp. zg-ZJ OX=2678504 GN=metN PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1680

0

1680

538985

37

2743

320.8

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.398 ± 0.066

0.794 ± 0.021

5.671 ± 0.093

6.943 ± 0.063

4.269 ± 0.053

6.078 ± 0.075

2.248 ± 0.031

7.779 ± 0.077

7.39 ± 0.059

9.608 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.033

4.589 ± 0.05

3.172 ± 0.058

4.153 ± 0.046

3.699 ± 0.044

6.002 ± 0.102

6.464 ± 0.073

7.375 ± 0.061

0.768 ± 0.018

4.017 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski