White clover cryptic virus 1 (isolate Boccardo/2004) (WCCV-1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus; White clover cryptic virus 1

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q64FP0|RDRP_WCCVB RNA-directed RNA polymerase OS=White clover cryptic virus 1 (isolate Boccardo/2004) OX=654934 PE=4 SV=1
MM1 pKa = 7.12NQDD4 pKa = 3.04TPLANLNGPEE14 pKa = 4.17VPSGNVPPANPPGRR28 pKa = 11.84TNVAPPAQGAVQQPPAPAARR48 pKa = 11.84RR49 pKa = 11.84ARR51 pKa = 11.84NPHH54 pKa = 6.1GPVPPAGPSRR64 pKa = 11.84SAGAPAMLEE73 pKa = 3.92LSAAYY78 pKa = 9.4PMYY81 pKa = 9.88TEE83 pKa = 4.08QRR85 pKa = 11.84RR86 pKa = 11.84APNYY90 pKa = 8.66YY91 pKa = 10.39VPDD94 pKa = 3.96AQLLFHH100 pKa = 6.59TLGVCDD106 pKa = 4.23QLMTTTDD113 pKa = 3.01RR114 pKa = 11.84FLRR117 pKa = 11.84SMPSWLPIVSQLYY130 pKa = 10.48VSVLWNVMILKK141 pKa = 10.19VYY143 pKa = 10.81VNTGYY148 pKa = 10.8GAAYY152 pKa = 10.27AHH154 pKa = 7.04DD155 pKa = 5.25LDD157 pKa = 4.43VLLNHH162 pKa = 6.57LQINEE167 pKa = 3.97CMIPGPLVPFFQSLAAVNGPFDD189 pKa = 4.6WIGDD193 pKa = 4.56IIPAMPSFTEE203 pKa = 3.98LWTDD207 pKa = 3.29EE208 pKa = 4.49FAPHH212 pKa = 6.54AAYY215 pKa = 10.1ARR217 pKa = 11.84QIPIPAILLDD227 pKa = 3.38QLYY230 pKa = 10.04RR231 pKa = 11.84FATLAFDD238 pKa = 4.78AQLQTNYY245 pKa = 9.32ATFEE249 pKa = 3.98WYY251 pKa = 10.88SNIFNQDD258 pKa = 3.01VNTHH262 pKa = 5.36NARR265 pKa = 11.84LRR267 pKa = 11.84LGPQLCGSLFTTQAQCDD284 pKa = 3.79SARR287 pKa = 11.84AFWNPAFANGFTRR300 pKa = 11.84IDD302 pKa = 3.56AANGPLMAFPQLLGFISQDD321 pKa = 3.29GALQSNWFMHH331 pKa = 6.72ISLIMHH337 pKa = 7.35KK338 pKa = 10.0YY339 pKa = 8.1AQYY342 pKa = 11.07FNGSVPLKK350 pKa = 10.23SISPVGIGASVIYY363 pKa = 7.38GTPLEE368 pKa = 4.45DD369 pKa = 3.29TNVRR373 pKa = 11.84DD374 pKa = 3.6WLYY377 pKa = 10.25PAAAAIAPFRR387 pKa = 11.84STRR390 pKa = 11.84FLPRR394 pKa = 11.84RR395 pKa = 11.84EE396 pKa = 4.25LPATLAVRR404 pKa = 11.84FAHH407 pKa = 6.83ADD409 pKa = 3.46HH410 pKa = 6.93EE411 pKa = 4.45IEE413 pKa = 4.27EE414 pKa = 4.22QAEE417 pKa = 4.28QYY419 pKa = 11.08SILCHH424 pKa = 6.29TNMKK428 pKa = 9.62WYY430 pKa = 10.66VNNATQNNHH439 pKa = 4.48TAIEE443 pKa = 4.25GNYY446 pKa = 8.51IHH448 pKa = 6.78QGEE451 pKa = 4.68YY452 pKa = 9.67WNFTPFRR459 pKa = 11.84YY460 pKa = 9.92SPPVSLKK467 pKa = 8.41TQFAQVIASRR477 pKa = 11.84YY478 pKa = 7.5HH479 pKa = 4.14QQAANRR485 pKa = 11.84AEE487 pKa = 3.91

Molecular weight: 54.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q64FP0|RDRP_WCCVB RNA-directed RNA polymerase OS=White clover cryptic virus 1 (isolate Boccardo/2004) OX=654934 PE=4 SV=1
MM1 pKa = 8.36DD2 pKa = 4.02YY3 pKa = 11.07LITAFNRR10 pKa = 11.84ITHH13 pKa = 6.73WFLTPTNLEE22 pKa = 4.34YY23 pKa = 10.35IGSYY27 pKa = 10.09SLPPGLLRR35 pKa = 11.84VNDD38 pKa = 3.61VAVANHH44 pKa = 6.96KK45 pKa = 9.18ATLDD49 pKa = 3.52RR50 pKa = 11.84SFDD53 pKa = 3.3KK54 pKa = 11.0YY55 pKa = 11.08LYY57 pKa = 8.91EE58 pKa = 5.05HH59 pKa = 7.76EE60 pKa = 4.75INLITKK66 pKa = 7.7EE67 pKa = 4.0YY68 pKa = 9.45RR69 pKa = 11.84RR70 pKa = 11.84SPIDD74 pKa = 3.37EE75 pKa = 4.61DD76 pKa = 4.94SILEE80 pKa = 4.22DD81 pKa = 4.41FFSGDD86 pKa = 3.14LPYY89 pKa = 10.76FEE91 pKa = 5.74IPFDD95 pKa = 3.56EE96 pKa = 4.49HH97 pKa = 7.54VEE99 pKa = 4.07RR100 pKa = 11.84GLEE103 pKa = 3.88CMAAAFRR110 pKa = 11.84PPRR113 pKa = 11.84PCRR116 pKa = 11.84PAHH119 pKa = 6.19ILDD122 pKa = 3.78VKK124 pKa = 10.61HH125 pKa = 6.44GYY127 pKa = 8.11PYY129 pKa = 10.43KK130 pKa = 10.06WNVNAEE136 pKa = 4.36PPFSTDD142 pKa = 3.4EE143 pKa = 4.16YY144 pKa = 10.97FLSQRR149 pKa = 11.84KK150 pKa = 6.69TFGEE154 pKa = 5.0FIRR157 pKa = 11.84MHH159 pKa = 6.57EE160 pKa = 4.18YY161 pKa = 10.58EE162 pKa = 5.75HH163 pKa = 7.05IDD165 pKa = 3.17KK166 pKa = 10.9EE167 pKa = 4.62DD168 pKa = 3.31FFRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 5.9PNIEE177 pKa = 3.71SHH179 pKa = 7.13DD180 pKa = 3.9FLRR183 pKa = 11.84TVVPPKK189 pKa = 10.59FGFLKK194 pKa = 11.0SMIFSWTRR202 pKa = 11.84RR203 pKa = 11.84WHH205 pKa = 6.51HH206 pKa = 6.39IIKK209 pKa = 10.57SGFQDD214 pKa = 3.54STDD217 pKa = 3.88LEE219 pKa = 4.27QTGYY223 pKa = 10.41FFNRR227 pKa = 11.84FIFPMLLHH235 pKa = 6.66TKK237 pKa = 8.3TAIVKK242 pKa = 10.57KK243 pKa = 10.26NDD245 pKa = 3.58PNKK248 pKa = 9.91MRR250 pKa = 11.84TIWGASKK257 pKa = 9.78PWIIAEE263 pKa = 4.1TMFYY267 pKa = 10.21WEE269 pKa = 3.66YY270 pKa = 10.78LAWIKK275 pKa = 10.55HH276 pKa = 5.35NPGATPMLWGYY287 pKa = 7.52EE288 pKa = 4.17TFTGGWFRR296 pKa = 11.84LNHH299 pKa = 6.01EE300 pKa = 4.88LFCGLIQRR308 pKa = 11.84SFLTLDD314 pKa = 3.43WSRR317 pKa = 11.84FDD319 pKa = 3.15KK320 pKa = 10.78RR321 pKa = 11.84AYY323 pKa = 10.14FPLLRR328 pKa = 11.84RR329 pKa = 11.84ILYY332 pKa = 7.1TVKK335 pKa = 9.88TFLTFEE341 pKa = 4.03EE342 pKa = 6.24GYY344 pKa = 10.49VPTHH348 pKa = 6.99AAPTHH353 pKa = 5.51PQWSQEE359 pKa = 4.05NIDD362 pKa = 3.57RR363 pKa = 11.84LEE365 pKa = 4.18RR366 pKa = 11.84LWLWTLEE373 pKa = 4.18NLFEE377 pKa = 4.94APIILPDD384 pKa = 2.89GRR386 pKa = 11.84MYY388 pKa = 10.65RR389 pKa = 11.84RR390 pKa = 11.84HH391 pKa = 5.78FAGIPSGLFITQLLDD406 pKa = 2.7SWYY409 pKa = 9.85NYY411 pKa = 9.3TMLATILSALHH422 pKa = 6.9FDD424 pKa = 4.25PLHH427 pKa = 6.76CIIKK431 pKa = 9.93VQGDD435 pKa = 3.68DD436 pKa = 3.67SILRR440 pKa = 11.84LTTLIPVDD448 pKa = 3.23QHH450 pKa = 5.67TNFMDD455 pKa = 4.66HH456 pKa = 6.98IVRR459 pKa = 11.84LADD462 pKa = 3.4TYY464 pKa = 11.33FNSIVNVKK472 pKa = 10.09KK473 pKa = 10.93SEE475 pKa = 3.85VRR477 pKa = 11.84NSLNGCEE484 pKa = 3.92VLSYY488 pKa = 10.62RR489 pKa = 11.84NHH491 pKa = 6.48NGLPHH496 pKa = 6.86RR497 pKa = 11.84DD498 pKa = 4.22EE499 pKa = 4.25ITMLAQFYY507 pKa = 7.85HH508 pKa = 6.12TKK510 pKa = 10.61ARR512 pKa = 11.84DD513 pKa = 3.58PTPEE517 pKa = 3.33ITMAQAIGFAYY528 pKa = 10.24ASCANHH534 pKa = 6.33NRR536 pKa = 11.84VLWVLEE542 pKa = 3.86DD543 pKa = 3.44VYY545 pKa = 11.58NYY547 pKa = 10.55YY548 pKa = 10.35RR549 pKa = 11.84DD550 pKa = 3.28LGYY553 pKa = 10.61RR554 pKa = 11.84PNRR557 pKa = 11.84AGLTLTFGDD566 pKa = 4.55SPDD569 pKa = 3.29LTMPEE574 pKa = 4.14MPLDD578 pKa = 3.84HH579 pKa = 7.26FPTKK583 pKa = 10.6SEE585 pKa = 2.97IRR587 pKa = 11.84RR588 pKa = 11.84YY589 pKa = 8.33HH590 pKa = 6.22TEE592 pKa = 3.07THH594 pKa = 5.74YY595 pKa = 11.25QNEE598 pKa = 4.3AQNARR603 pKa = 11.84TWPRR607 pKa = 11.84TLFINAPGEE616 pKa = 4.0

Molecular weight: 72.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1103

487

616

551.5

63.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.522 ± 2.233

0.997 ± 0.016

4.805 ± 0.619

4.896 ± 0.898

6.165 ± 0.575

4.714 ± 0.234

3.898 ± 0.571

5.802 ± 0.488

2.539 ± 0.843

9.157 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.009

5.621 ± 0.53

7.797 ± 0.92

3.898 ± 1.261

6.074 ± 0.639

4.805 ± 0.183

6.437 ± 0.612

4.17 ± 0.537

2.448 ± 0.22

4.805 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski