Methanomethylophilus alvus (strain Mx1201)

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; Candidatus Methanomethylophilaceae; Candidatus Methanomethylophilus; Candidatus Methanomethylophilus alvus

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9SGW0|M9SGW0_METAX Branched-chain-amino-acid aminotransferase OS=Methanomethylophilus alvus (strain Mx1201) OX=1236689 GN=ilvE PE=3 SV=1
MM1 pKa = 6.93KK2 pKa = 9.2TVSGAFVIVATLAISALVLTGGAYY26 pKa = 10.67SLTSSVDD33 pKa = 2.92ISGNQMLSDD42 pKa = 4.52GYY44 pKa = 11.62VLDD47 pKa = 4.64IYY49 pKa = 11.18SDD51 pKa = 3.91TEE53 pKa = 4.08GTTLSDD59 pKa = 3.43GKK61 pKa = 8.91TLFADD66 pKa = 3.22ANIYY70 pKa = 9.62YY71 pKa = 8.4RR72 pKa = 11.84TVGDD76 pKa = 3.8TVNVSSEE83 pKa = 3.94KK84 pKa = 10.96GNVGSDD90 pKa = 3.65PNHH93 pKa = 6.27SLSAVKK99 pKa = 10.04EE100 pKa = 3.88DD101 pKa = 3.9RR102 pKa = 11.84YY103 pKa = 11.64LMISSSGSDD112 pKa = 3.73GISVEE117 pKa = 4.92CRR119 pKa = 11.84MKK121 pKa = 11.02DD122 pKa = 3.42PGSGEE127 pKa = 4.2YY128 pKa = 10.63VSLGLSDD135 pKa = 4.13IPPFISSLEE144 pKa = 4.08VVLTNGTDD152 pKa = 3.34SYY154 pKa = 11.88SVTLYY159 pKa = 10.76DD160 pKa = 4.49LEE162 pKa = 4.47TSVVSGKK169 pKa = 10.74ASFEE173 pKa = 4.07GLSMVPGEE181 pKa = 4.34YY182 pKa = 10.25YY183 pKa = 10.6AISVDD188 pKa = 3.16ITLKK192 pKa = 10.61DD193 pKa = 3.71VPVSSDD199 pKa = 4.19DD200 pKa = 3.33SWKK203 pKa = 10.98DD204 pKa = 3.36SAPSFSFIFAAEE216 pKa = 3.87AGAA219 pKa = 4.2

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9SJD7|M9SJD7_METAX Uncharacterized protein OS=Methanomethylophilus alvus (strain Mx1201) OX=1236689 GN=MMALV_14850 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 9.88PKK4 pKa = 10.03KK5 pKa = 10.17QYY7 pKa = 10.21GRR9 pKa = 11.84QVGCDD14 pKa = 2.55RR15 pKa = 11.84CGRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84GIIRR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 10.09GMHH29 pKa = 7.66LCRR32 pKa = 11.84QCFRR36 pKa = 11.84DD37 pKa = 3.69MAPEE41 pKa = 4.41LGFKK45 pKa = 10.27KK46 pKa = 10.81YY47 pKa = 10.16SS48 pKa = 3.24

Molecular weight: 5.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1643

0

1643

490742

37

2373

298.7

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.106 ± 0.072

1.692 ± 0.03

7.014 ± 0.05

6.835 ± 0.067

3.737 ± 0.04

8.117 ± 0.07

1.65 ± 0.025

6.638 ± 0.059

5.851 ± 0.07

7.943 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.564 ± 0.035

3.372 ± 0.042

3.885 ± 0.039

2.158 ± 0.035

5.496 ± 0.069

6.412 ± 0.064

5.187 ± 0.053

7.863 ± 0.06

0.903 ± 0.02

3.572 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski