Allochromatium warmingii (Chromatium warmingii)
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2689 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3EAA2|A0A1H3EAA2_ALLWA Multifunctional CCA protein OS=Allochromatium warmingii OX=61595 GN=cca PE=3 SV=1
MM1 pKa = 7.17 TVASDD6 pKa = 3.72 LLEE9 pKa = 4.87 GYY11 pKa = 10.32 EE12 pKa = 4.55 SIPVEE17 pKa = 4.18 NRR19 pKa = 11.84 NEE21 pKa = 3.78 ATQQFIQILNEE32 pKa = 3.95 MPEE35 pKa = 4.1 DD36 pKa = 3.81 ADD38 pKa = 3.9 FVEE41 pKa = 6.07 GPTNSQPGEE50 pKa = 3.98 TSYY53 pKa = 12.06 SLLPPPAQGEE63 pKa = 4.36 TGQAEE68 pKa = 4.4 GGDD71 pKa = 4.17 GYY73 pKa = 11.14 DD74 pKa = 5.14 IIITDD79 pKa = 4.87 LPPDD83 pKa = 3.85 TEE85 pKa = 4.77 SDD87 pKa = 3.55 FTVANNVEE95 pKa = 3.97 QFIILGSGSANLQQEE110 pKa = 4.49 TPPPGNQQPTQFFTGNGDD128 pKa = 3.6 DD129 pKa = 3.96 SLLTGGAGSQGVFGGGDD146 pKa = 3.53 DD147 pKa = 3.88 TVTSATAVTGQNPTMSQMTLINGTAAAKK175 pKa = 8.78 WWDD178 pKa = 3.51 EE179 pKa = 3.76 ADD181 pKa = 3.72 YY182 pKa = 10.95 LANNPDD188 pKa = 2.97 VAQAVQNGALVSGYY202 pKa = 7.02 QHH204 pKa = 6.84 YY205 pKa = 10.7 ILFGAAEE212 pKa = 4.25 NRR214 pKa = 11.84 DD215 pKa = 3.85 STVSSWWNEE224 pKa = 3.37 GYY226 pKa = 11.13 YY227 pKa = 10.44 LDD229 pKa = 4.86 NNPDD233 pKa = 3.07 VAQAIQAGILNSGYY247 pKa = 7.71 QHH249 pKa = 6.65 FALYY253 pKa = 9.96 GAAEE257 pKa = 4.19 NRR259 pKa = 11.84 VSTVSTWWNEE269 pKa = 3.26 TDD271 pKa = 3.76 YY272 pKa = 11.68 LNNNPDD278 pKa = 2.84 VAQAVQNGLLASGYY292 pKa = 6.97 QHH294 pKa = 6.88 FAMFGASEE302 pKa = 4.07 NRR304 pKa = 11.84 NLIRR308 pKa = 11.84 SWWDD312 pKa = 2.84 EE313 pKa = 3.65 EE314 pKa = 5.52 LYY316 pKa = 10.98 LKK318 pKa = 10.81 EE319 pKa = 4.17 NTDD322 pKa = 3.32 VAQAVADD329 pKa = 4.2 GVFASGYY336 pKa = 9.59 HH337 pKa = 5.55 HH338 pKa = 6.96 FAMYY342 pKa = 10.05 GAAEE346 pKa = 3.97 QRR348 pKa = 11.84 DD349 pKa = 3.54 PNAYY353 pKa = 10.12 FNAADD358 pKa = 3.7 YY359 pKa = 10.67 LAANGDD365 pKa = 3.12 VDD367 pKa = 3.48 IAVQIGIFKK376 pKa = 10.72 SAFEE380 pKa = 4.7 HH381 pKa = 5.04 YY382 pKa = 9.54 QKK384 pKa = 11.13 YY385 pKa = 9.12 GAAEE389 pKa = 4.1 GRR391 pKa = 11.84 TPSDD395 pKa = 3.3 DD396 pKa = 4.16 FNAEE400 pKa = 3.87 NYY402 pKa = 9.87 LDD404 pKa = 3.52 RR405 pKa = 11.84 WQDD408 pKa = 3.23 VEE410 pKa = 4.85 DD411 pKa = 3.91 AVNNGSLNAGQHH423 pKa = 4.89 AVFYY427 pKa = 9.89 GATEE431 pKa = 4.12 GRR433 pKa = 11.84 VSNDD437 pKa = 2.25 VDD439 pKa = 2.9 IWEE442 pKa = 4.9 GGAGNDD448 pKa = 4.1 TLDD451 pKa = 4.47 GGLGDD456 pKa = 5.69 DD457 pKa = 5.11 DD458 pKa = 4.25 LTGGTGSDD466 pKa = 3.19 TFVFRR471 pKa = 11.84 ANSDD475 pKa = 2.95 EE476 pKa = 5.16 DD477 pKa = 3.68 IIRR480 pKa = 11.84 DD481 pKa = 3.71 FDD483 pKa = 4.38 FKK485 pKa = 10.98 TSATDD490 pKa = 3.69 LEE492 pKa = 4.85 KK493 pKa = 10.83 DD494 pKa = 3.69 TIQTIGLNVTAEE506 pKa = 4.16 SLVAGVTEE514 pKa = 4.54 DD515 pKa = 3.84 ANGHH519 pKa = 5.52 AVVTLSDD526 pKa = 3.84 GNTITLIGVKK536 pKa = 10.09 AADD539 pKa = 3.52 VTADD543 pKa = 3.61 MFDD546 pKa = 3.33 IQPP549 pKa = 3.51
Molecular weight: 58.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.478
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.389
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.05
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.821
Patrickios 1.113
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A1H3BYQ7|A0A1H3BYQ7_ALLWA HD-like signal output (HDOD) domain no enzymatic activity OS=Allochromatium warmingii OX=61595 GN=SAMN05421644_10457 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LKK11 pKa = 10.48 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 SATRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 9.31 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.62 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LIPP44 pKa = 3.91
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2689
0
2689
889806
24
4262
330.9
36.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.627 ± 0.061
1.063 ± 0.019
5.646 ± 0.042
6.018 ± 0.041
3.405 ± 0.031
7.145 ± 0.048
2.468 ± 0.024
5.271 ± 0.036
2.748 ± 0.044
11.77 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.019 ± 0.025
2.78 ± 0.036
5.161 ± 0.042
4.398 ± 0.037
7.43 ± 0.05
5.027 ± 0.033
5.51 ± 0.044
6.702 ± 0.041
1.384 ± 0.022
2.427 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here