Gordonia phage Trine

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Trinevirus; Gordonia virus Trine

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q906|A0A2Z4Q906_9CAUD Membrane protein OS=Gordonia phage Trine OX=2201431 GN=29 PE=4 SV=1
MM1 pKa = 7.43TEE3 pKa = 3.73IRR5 pKa = 11.84CEE7 pKa = 4.11CGHH10 pKa = 7.17PEE12 pKa = 4.11STHH15 pKa = 5.34PTDD18 pKa = 3.39PVEE21 pKa = 4.81NVDD24 pKa = 5.96AMGPWWRR31 pKa = 11.84YY32 pKa = 8.56CLGVHH37 pKa = 6.51GFFVDD42 pKa = 4.7SDD44 pKa = 4.16PSPCRR49 pKa = 11.84CTGFTEE55 pKa = 4.39PTAEE59 pKa = 4.1PVFLVPDD66 pKa = 4.21DD67 pKa = 4.82EE68 pKa = 5.81LPEE71 pKa = 4.31LPGGEE76 pKa = 3.94ITLTDD81 pKa = 4.16PEE83 pKa = 4.45DD84 pKa = 3.56KK85 pKa = 11.04ARR87 pKa = 4.02

Molecular weight: 9.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q916|A0A2Z4Q916_9CAUD Terminase large subunit OS=Gordonia phage Trine OX=2201431 GN=2 PE=4 SV=1
MM1 pKa = 6.9TQHH4 pKa = 7.33DD5 pKa = 4.51DD6 pKa = 3.82EE7 pKa = 5.2PRR9 pKa = 11.84EE10 pKa = 4.14TPSYY14 pKa = 10.4VPFMPSAPYY23 pKa = 9.97RR24 pKa = 11.84PPRR27 pKa = 11.84SQRR30 pKa = 11.84RR31 pKa = 11.84ARR33 pKa = 11.84AQLRR37 pKa = 11.84GRR39 pKa = 11.84AQRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84QRR46 pKa = 11.84RR47 pKa = 11.84ATALVKK53 pKa = 10.39EE54 pKa = 4.84LGLDD58 pKa = 3.81QPWKK62 pKa = 10.3RR63 pKa = 11.84PQLADD68 pKa = 3.59DD69 pKa = 5.0EE70 pKa = 5.58GPGSQDD76 pKa = 2.66

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13999

39

1412

212.1

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.215 ± 0.458

0.564 ± 0.097

6.386 ± 0.279

6.443 ± 0.296

2.343 ± 0.143

8.393 ± 0.395

1.836 ± 0.191

4.036 ± 0.195

3.407 ± 0.233

8.436 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.132

2.607 ± 0.209

5.95 ± 0.306

3.407 ± 0.168

7.093 ± 0.458

5.822 ± 0.33

7.265 ± 0.27

7.386 ± 0.241

1.964 ± 0.154

2.2 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski