Amino acid dipepetide frequency for Gordonia phage Trine

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.074AlaAla: 17.074 ± 1.333
0.429AlaCys: 0.429 ± 0.164
7.358AlaAsp: 7.358 ± 1.011
10.43AlaGlu: 10.43 ± 1.159
2.643AlaPhe: 2.643 ± 0.411
9.501AlaGly: 9.501 ± 1.154
1.5AlaHis: 1.5 ± 0.453
4.501AlaIle: 4.501 ± 0.701
5.644AlaLys: 5.644 ± 0.758
9.287AlaLeu: 9.287 ± 0.693
3.215AlaMet: 3.215 ± 0.463
3.286AlaAsn: 3.286 ± 0.447
5.429AlaPro: 5.429 ± 0.733
4.001AlaGln: 4.001 ± 0.461
9.358AlaArg: 9.358 ± 1.068
6.858AlaSer: 6.858 ± 0.739
7.858AlaThr: 7.858 ± 0.842
8.073AlaVal: 8.073 ± 0.833
3.072AlaTrp: 3.072 ± 0.426
2.643AlaTyr: 2.643 ± 0.466
0.0AlaXaa: 0.0 ± 0.0
Cys
0.286CysAla: 0.286 ± 0.149
0.0CysCys: 0.0 ± 0.0
0.5CysAsp: 0.5 ± 0.183
0.286CysGlu: 0.286 ± 0.143
0.214CysPhe: 0.214 ± 0.109
0.786CysGly: 0.786 ± 0.29
0.071CysHis: 0.071 ± 0.076
0.143CysIle: 0.143 ± 0.103
0.0CysLys: 0.0 ± 0.0
0.572CysLeu: 0.572 ± 0.18
0.0CysMet: 0.0 ± 0.0
0.5CysAsn: 0.5 ± 0.203
0.429CysPro: 0.429 ± 0.169
0.357CysGln: 0.357 ± 0.171
0.572CysArg: 0.572 ± 0.208
0.5CysSer: 0.5 ± 0.206
0.214CysThr: 0.214 ± 0.108
0.071CysVal: 0.071 ± 0.073
0.071CysTrp: 0.071 ± 0.08
0.071CysTyr: 0.071 ± 0.06
0.0CysXaa: 0.0 ± 0.0
Asp
7.001AspAla: 7.001 ± 0.644
0.071AspCys: 0.071 ± 0.075
3.929AspAsp: 3.929 ± 0.826
2.715AspGlu: 2.715 ± 0.498
0.929AspPhe: 0.929 ± 0.281
5.572AspGly: 5.572 ± 0.541
1.643AspHis: 1.643 ± 0.351
2.143AspIle: 2.143 ± 0.433
1.5AspLys: 1.5 ± 0.359
6.215AspLeu: 6.215 ± 0.833
1.929AspMet: 1.929 ± 0.313
1.715AspAsn: 1.715 ± 0.361
5.644AspPro: 5.644 ± 0.631
1.929AspGln: 1.929 ± 0.386
5.072AspArg: 5.072 ± 0.77
2.429AspSer: 2.429 ± 0.386
5.644AspThr: 5.644 ± 0.854
4.215AspVal: 4.215 ± 0.526
1.786AspTrp: 1.786 ± 0.364
1.786AspTyr: 1.786 ± 0.402
0.0AspXaa: 0.0 ± 0.0
Glu
8.787GluAla: 8.787 ± 0.878
0.357GluCys: 0.357 ± 0.193
4.929GluAsp: 4.929 ± 0.707
4.858GluGlu: 4.858 ± 0.658
1.5GluPhe: 1.5 ± 0.441
5.286GluGly: 5.286 ± 0.566
0.786GluHis: 0.786 ± 0.196
3.929GluIle: 3.929 ± 0.544
1.286GluLys: 1.286 ± 0.303
7.287GluLeu: 7.287 ± 0.67
1.0GluMet: 1.0 ± 0.36
1.357GluAsn: 1.357 ± 0.353
3.143GluPro: 3.143 ± 0.623
1.715GluGln: 1.715 ± 0.397
4.001GluArg: 4.001 ± 0.538
3.215GluSer: 3.215 ± 0.508
3.643GluThr: 3.643 ± 0.533
4.072GluVal: 4.072 ± 0.629
1.429GluTrp: 1.429 ± 0.379
1.857GluTyr: 1.857 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
2.929PheAla: 2.929 ± 0.413
0.143PheCys: 0.143 ± 0.097
2.143PheAsp: 2.143 ± 0.348
0.929PheGlu: 0.929 ± 0.268
0.286PhePhe: 0.286 ± 0.134
1.715PheGly: 1.715 ± 0.319
0.643PheHis: 0.643 ± 0.244
0.786PheIle: 0.786 ± 0.191
0.786PheLys: 0.786 ± 0.194
1.786PheLeu: 1.786 ± 0.394
0.5PheMet: 0.5 ± 0.185
0.572PheAsn: 0.572 ± 0.235
1.643PhePro: 1.643 ± 0.371
1.286PheGln: 1.286 ± 0.428
1.5PheArg: 1.5 ± 0.328
1.572PheSer: 1.572 ± 0.393
2.286PheThr: 2.286 ± 0.411
1.143PheVal: 1.143 ± 0.326
0.143PheTrp: 0.143 ± 0.091
0.643PheTyr: 0.643 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
7.215GlyAla: 7.215 ± 0.857
0.429GlyCys: 0.429 ± 0.185
5.072GlyAsp: 5.072 ± 0.554
5.001GlyGlu: 5.001 ± 0.682
2.643GlyPhe: 2.643 ± 0.368
6.715GlyGly: 6.715 ± 0.901
1.5GlyHis: 1.5 ± 0.334
4.286GlyIle: 4.286 ± 0.921
3.501GlyLys: 3.501 ± 0.567
7.215GlyLeu: 7.215 ± 0.665
1.715GlyMet: 1.715 ± 0.4
2.143GlyAsn: 2.143 ± 0.439
4.429GlyPro: 4.429 ± 0.705
3.501GlyGln: 3.501 ± 0.422
5.572GlyArg: 5.572 ± 0.717
6.072GlySer: 6.072 ± 0.911
6.215GlyThr: 6.215 ± 0.651
6.715GlyVal: 6.715 ± 0.899
1.786GlyTrp: 1.786 ± 0.266
2.215GlyTyr: 2.215 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.715HisAla: 1.715 ± 0.422
0.071HisCys: 0.071 ± 0.067
1.5HisAsp: 1.5 ± 0.36
0.429HisGlu: 0.429 ± 0.168
0.643HisPhe: 0.643 ± 0.214
1.929HisGly: 1.929 ± 0.473
0.786HisHis: 0.786 ± 0.242
0.714HisIle: 0.714 ± 0.226
0.5HisLys: 0.5 ± 0.149
1.715HisLeu: 1.715 ± 0.401
0.429HisMet: 0.429 ± 0.169
0.572HisAsn: 0.572 ± 0.246
2.0HisPro: 2.0 ± 0.55
1.0HisGln: 1.0 ± 0.309
1.572HisArg: 1.572 ± 0.381
0.429HisSer: 0.429 ± 0.159
0.857HisThr: 0.857 ± 0.24
1.0HisVal: 1.0 ± 0.341
0.286HisTrp: 0.286 ± 0.154
0.143HisTyr: 0.143 ± 0.107
0.0HisXaa: 0.0 ± 0.0
Ile
3.572IleAla: 3.572 ± 0.534
0.071IleCys: 0.071 ± 0.088
1.929IleAsp: 1.929 ± 0.383
2.715IleGlu: 2.715 ± 0.361
0.929IlePhe: 0.929 ± 0.257
3.572IleGly: 3.572 ± 0.706
1.214IleHis: 1.214 ± 0.271
1.214IleIle: 1.214 ± 0.241
1.857IleLys: 1.857 ± 0.426
3.143IleLeu: 3.143 ± 0.375
0.929IleMet: 0.929 ± 0.338
1.143IleAsn: 1.143 ± 0.288
3.143IlePro: 3.143 ± 0.5
2.215IleGln: 2.215 ± 0.316
3.429IleArg: 3.429 ± 0.375
2.643IleSer: 2.643 ± 0.444
3.143IleThr: 3.143 ± 0.478
2.143IleVal: 2.143 ± 0.312
0.857IleTrp: 0.857 ± 0.248
0.5IleTyr: 0.5 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
5.787LysAla: 5.787 ± 0.931
0.214LysCys: 0.214 ± 0.121
2.143LysAsp: 2.143 ± 0.38
2.357LysGlu: 2.357 ± 0.381
0.5LysPhe: 0.5 ± 0.203
2.143LysGly: 2.143 ± 0.467
0.357LysHis: 0.357 ± 0.199
2.072LysIle: 2.072 ± 0.382
0.286LysLys: 0.286 ± 0.15
3.786LysLeu: 3.786 ± 0.616
0.429LysMet: 0.429 ± 0.14
0.643LysAsn: 0.643 ± 0.217
1.072LysPro: 1.072 ± 0.287
0.5LysGln: 0.5 ± 0.235
1.929LysArg: 1.929 ± 0.384
2.786LysSer: 2.786 ± 0.425
2.858LysThr: 2.858 ± 0.568
2.572LysVal: 2.572 ± 0.494
0.857LysTrp: 0.857 ± 0.222
0.786LysTyr: 0.786 ± 0.24
0.0LysXaa: 0.0 ± 0.0
Leu
11.144LeuAla: 11.144 ± 1.009
0.714LeuCys: 0.714 ± 0.287
5.929LeuAsp: 5.929 ± 0.639
5.429LeuGlu: 5.429 ± 0.499
1.429LeuPhe: 1.429 ± 0.293
8.93LeuGly: 8.93 ± 0.603
1.429LeuHis: 1.429 ± 0.31
3.0LeuIle: 3.0 ± 0.416
3.572LeuLys: 3.572 ± 0.485
6.072LeuLeu: 6.072 ± 0.586
1.5LeuMet: 1.5 ± 0.347
1.429LeuAsn: 1.429 ± 0.343
4.929LeuPro: 4.929 ± 0.627
2.715LeuGln: 2.715 ± 0.462
6.858LeuArg: 6.858 ± 0.836
4.143LeuSer: 4.143 ± 0.465
7.001LeuThr: 7.001 ± 0.767
4.572LeuVal: 4.572 ± 0.543
1.5LeuTrp: 1.5 ± 0.339
2.072LeuTyr: 2.072 ± 0.337
0.0LeuXaa: 0.0 ± 0.0
Met
4.358MetAla: 4.358 ± 0.644
0.071MetCys: 0.071 ± 0.079
1.429MetAsp: 1.429 ± 0.328
1.286MetGlu: 1.286 ± 0.249
0.5MetPhe: 0.5 ± 0.203
1.072MetGly: 1.072 ± 0.272
0.286MetHis: 0.286 ± 0.125
0.857MetIle: 0.857 ± 0.235
0.714MetLys: 0.714 ± 0.193
1.143MetLeu: 1.143 ± 0.264
0.214MetMet: 0.214 ± 0.127
1.0MetAsn: 1.0 ± 0.324
1.214MetPro: 1.214 ± 0.378
0.429MetGln: 0.429 ± 0.175
0.929MetArg: 0.929 ± 0.235
1.715MetSer: 1.715 ± 0.299
3.0MetThr: 3.0 ± 0.545
1.857MetVal: 1.857 ± 0.299
0.0MetTrp: 0.0 ± 0.0
0.357MetTyr: 0.357 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
4.143AsnAla: 4.143 ± 0.55
0.071AsnCys: 0.071 ± 0.07
0.714AsnAsp: 0.714 ± 0.212
1.214AsnGlu: 1.214 ± 0.3
0.857AsnPhe: 0.857 ± 0.27
2.572AsnGly: 2.572 ± 0.557
0.286AsnHis: 0.286 ± 0.152
1.0AsnIle: 1.0 ± 0.293
0.714AsnLys: 0.714 ± 0.229
2.0AsnLeu: 2.0 ± 0.43
0.357AsnMet: 0.357 ± 0.172
0.929AsnAsn: 0.929 ± 0.259
2.286AsnPro: 2.286 ± 0.332
0.5AsnGln: 0.5 ± 0.19
1.429AsnArg: 1.429 ± 0.295
1.214AsnSer: 1.214 ± 0.278
1.857AsnThr: 1.857 ± 0.479
2.643AsnVal: 2.643 ± 0.458
0.643AsnTrp: 0.643 ± 0.19
0.643AsnTyr: 0.643 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
7.215ProAla: 7.215 ± 0.69
0.357ProCys: 0.357 ± 0.198
4.715ProAsp: 4.715 ± 0.519
5.501ProGlu: 5.501 ± 0.657
1.5ProPhe: 1.5 ± 0.26
5.501ProGly: 5.501 ± 0.766
0.929ProHis: 0.929 ± 0.246
2.786ProIle: 2.786 ± 0.382
1.429ProLys: 1.429 ± 0.373
3.572ProLeu: 3.572 ± 0.565
1.214ProMet: 1.214 ± 0.303
1.286ProAsn: 1.286 ± 0.28
2.357ProPro: 2.357 ± 0.535
1.072ProGln: 1.072 ± 0.285
3.143ProArg: 3.143 ± 0.625
4.429ProSer: 4.429 ± 0.73
4.501ProThr: 4.501 ± 0.747
5.929ProVal: 5.929 ± 0.851
1.143ProTrp: 1.143 ± 0.311
0.929ProTyr: 0.929 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
4.572GlnAla: 4.572 ± 0.794
0.143GlnCys: 0.143 ± 0.1
1.929GlnAsp: 1.929 ± 0.356
2.143GlnGlu: 2.143 ± 0.366
1.214GlnPhe: 1.214 ± 0.319
2.429GlnGly: 2.429 ± 0.326
0.857GlnHis: 0.857 ± 0.241
1.572GlnIle: 1.572 ± 0.311
1.0GlnLys: 1.0 ± 0.255
3.429GlnLeu: 3.429 ± 0.552
0.857GlnMet: 0.857 ± 0.278
1.143GlnAsn: 1.143 ± 0.324
1.429GlnPro: 1.429 ± 0.272
0.643GlnGln: 0.643 ± 0.255
3.215GlnArg: 3.215 ± 0.427
1.214GlnSer: 1.214 ± 0.307
1.929GlnThr: 1.929 ± 0.352
3.143GlnVal: 3.143 ± 0.444
0.714GlnTrp: 0.714 ± 0.244
0.5GlnTyr: 0.5 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
8.93ArgAla: 8.93 ± 1.046
0.643ArgCys: 0.643 ± 0.234
4.715ArgAsp: 4.715 ± 0.646
3.715ArgGlu: 3.715 ± 0.485
1.643ArgPhe: 1.643 ± 0.425
6.358ArgGly: 6.358 ± 0.884
1.643ArgHis: 1.643 ± 0.432
2.0ArgIle: 2.0 ± 0.407
2.0ArgLys: 2.0 ± 0.439
5.144ArgLeu: 5.144 ± 0.737
2.429ArgMet: 2.429 ± 0.45
2.215ArgAsn: 2.215 ± 0.372
3.501ArgPro: 3.501 ± 0.625
3.429ArgGln: 3.429 ± 0.447
5.429ArgArg: 5.429 ± 1.131
4.786ArgSer: 4.786 ± 0.537
4.286ArgThr: 4.286 ± 0.539
5.286ArgVal: 5.286 ± 0.741
1.643ArgTrp: 1.643 ± 0.43
1.143ArgTyr: 1.143 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
6.787SerAla: 6.787 ± 0.696
0.214SerCys: 0.214 ± 0.118
4.001SerAsp: 4.001 ± 0.598
2.715SerGlu: 2.715 ± 0.452
1.143SerPhe: 1.143 ± 0.23
5.358SerGly: 5.358 ± 0.665
1.357SerHis: 1.357 ± 0.357
1.857SerIle: 1.857 ± 0.363
1.715SerLys: 1.715 ± 0.388
4.715SerLeu: 4.715 ± 0.645
1.214SerMet: 1.214 ± 0.211
1.357SerAsn: 1.357 ± 0.215
4.715SerPro: 4.715 ± 0.637
3.072SerGln: 3.072 ± 0.45
4.001SerArg: 4.001 ± 0.508
3.215SerSer: 3.215 ± 0.578
3.929SerThr: 3.929 ± 0.585
4.143SerVal: 4.143 ± 0.467
1.143SerTrp: 1.143 ± 0.285
1.572SerTyr: 1.572 ± 0.374
0.0SerXaa: 0.0 ± 0.0
Thr
8.93ThrAla: 8.93 ± 0.771
0.714ThrCys: 0.714 ± 0.167
4.001ThrAsp: 4.001 ± 0.493
5.501ThrGlu: 5.501 ± 0.631
1.857ThrPhe: 1.857 ± 0.338
6.429ThrGly: 6.429 ± 0.73
1.072ThrHis: 1.072 ± 0.314
2.786ThrIle: 2.786 ± 0.531
3.715ThrLys: 3.715 ± 0.522
6.501ThrLeu: 6.501 ± 0.576
1.857ThrMet: 1.857 ± 0.365
1.929ThrAsn: 1.929 ± 0.426
4.858ThrPro: 4.858 ± 0.776
1.5ThrGln: 1.5 ± 0.32
3.286ThrArg: 3.286 ± 0.45
3.786ThrSer: 3.786 ± 0.585
4.786ThrThr: 4.786 ± 0.761
6.501ThrVal: 6.501 ± 0.646
1.286ThrTrp: 1.286 ± 0.347
1.357ThrTyr: 1.357 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
7.573ValAla: 7.573 ± 0.819
0.714ValCys: 0.714 ± 0.229
4.429ValAsp: 4.429 ± 0.508
4.644ValGlu: 4.644 ± 0.586
2.0ValPhe: 2.0 ± 0.326
4.929ValGly: 4.929 ± 0.55
1.143ValHis: 1.143 ± 0.306
3.358ValIle: 3.358 ± 0.465
2.786ValLys: 2.786 ± 0.55
6.215ValLeu: 6.215 ± 0.702
1.715ValMet: 1.715 ± 0.361
1.857ValAsn: 1.857 ± 0.34
4.143ValPro: 4.143 ± 0.68
2.429ValGln: 2.429 ± 0.44
6.001ValArg: 6.001 ± 0.677
4.929ValSer: 4.929 ± 0.67
5.858ValThr: 5.858 ± 0.596
6.215ValVal: 6.215 ± 0.752
0.857ValTrp: 0.857 ± 0.24
2.072ValTyr: 2.072 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
1.857TrpAla: 1.857 ± 0.331
0.143TrpCys: 0.143 ± 0.119
1.357TrpAsp: 1.357 ± 0.287
1.643TrpGlu: 1.643 ± 0.346
0.572TrpPhe: 0.572 ± 0.248
1.143TrpGly: 1.143 ± 0.285
0.572TrpHis: 0.572 ± 0.243
0.714TrpIle: 0.714 ± 0.265
0.572TrpLys: 0.572 ± 0.161
2.215TrpLeu: 2.215 ± 0.433
0.429TrpMet: 0.429 ± 0.248
0.357TrpAsn: 0.357 ± 0.155
1.143TrpPro: 1.143 ± 0.282
0.643TrpGln: 0.643 ± 0.228
1.715TrpArg: 1.715 ± 0.388
1.0TrpSer: 1.0 ± 0.318
1.643TrpThr: 1.643 ± 0.297
1.357TrpVal: 1.357 ± 0.366
0.214TrpTrp: 0.214 ± 0.128
0.357TrpTyr: 0.357 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.286TyrAla: 2.286 ± 0.52
0.071TyrCys: 0.071 ± 0.074
1.143TyrAsp: 1.143 ± 0.31
0.929TyrGlu: 0.929 ± 0.273
0.429TyrPhe: 0.429 ± 0.242
2.0TyrGly: 2.0 ± 0.439
0.286TyrHis: 0.286 ± 0.121
0.643TyrIle: 0.643 ± 0.231
0.5TyrLys: 0.5 ± 0.258
2.357TyrLeu: 2.357 ± 0.42
0.429TyrMet: 0.429 ± 0.211
0.643TyrAsn: 0.643 ± 0.199
2.0TyrPro: 2.0 ± 0.405
1.143TyrGln: 1.143 ± 0.31
1.929TyrArg: 1.929 ± 0.339
1.286TyrSer: 1.286 ± 0.33
1.143TyrThr: 1.143 ± 0.328
2.215TyrVal: 2.215 ± 0.359
0.214TyrTrp: 0.214 ± 0.114
0.357TyrTyr: 0.357 ± 0.148
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (13999 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski