Streptococcus phage Javan628

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BDH1|A0A4D6BDH1_9CAUD Integrase OS=Streptococcus phage Javan628 OX=2548287 GN=Javan628_0055 PE=3 SV=1
MM1 pKa = 7.27EE2 pKa = 4.97EE3 pKa = 4.78KK4 pKa = 10.44YY5 pKa = 10.86LEE7 pKa = 4.19YY8 pKa = 10.76ALEE11 pKa = 4.22HH12 pKa = 6.75LEE14 pKa = 4.31RR15 pKa = 11.84EE16 pKa = 4.4LDD18 pKa = 4.03IIDD21 pKa = 3.72NPYY24 pKa = 10.02VYY26 pKa = 10.14EE27 pKa = 4.06YY28 pKa = 11.32DD29 pKa = 3.46EE30 pKa = 5.08DD31 pKa = 4.95KK32 pKa = 11.5DD33 pKa = 3.7MEE35 pKa = 4.27VRR37 pKa = 11.84KK38 pKa = 10.44DD39 pKa = 3.17NPYY42 pKa = 10.75YY43 pKa = 10.73VDD45 pKa = 4.05GVHH48 pKa = 7.4DD49 pKa = 3.89SPYY52 pKa = 9.92YY53 pKa = 9.78RR54 pKa = 11.84SEE56 pKa = 3.88IAKK59 pKa = 9.79DD60 pKa = 3.28LHH62 pKa = 7.17DD63 pKa = 4.32IKK65 pKa = 11.41QMLGGG70 pKa = 3.79

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BGF6|A0A4D6BGF6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan628 OX=2548287 GN=Javan628_0033 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.19RR3 pKa = 11.84LKK5 pKa = 10.36VLKK8 pKa = 10.27LYY10 pKa = 10.68LKK12 pKa = 9.59QQIRR16 pKa = 11.84HH17 pKa = 5.89SPSQFAIHH25 pKa = 6.57GKK27 pKa = 9.79ISLKK31 pKa = 10.7NVIQGRR37 pKa = 11.84TQADD41 pKa = 3.5DD42 pKa = 4.33FKK44 pKa = 11.12EE45 pKa = 3.97KK46 pKa = 9.72MYY48 pKa = 10.92KK49 pKa = 9.97AVHH52 pKa = 6.25KK53 pKa = 9.22KK54 pKa = 7.76TLYY57 pKa = 9.54VWW59 pKa = 3.68

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11399

45

1126

207.3

23.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.422 ± 0.778

0.553 ± 0.108

6.658 ± 0.471

7.659 ± 0.68

4.149 ± 0.238

6.194 ± 0.52

1.439 ± 0.142

7.325 ± 0.273

8.229 ± 0.479

8.308 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.781 ± 0.261

5.351 ± 0.278

2.667 ± 0.181

3.834 ± 0.255

4.053 ± 0.316

6.123 ± 0.529

5.834 ± 0.402

6.264 ± 0.245

1.053 ± 0.122

4.106 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski