Planctomycetes bacterium FF011L

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Roseimaritima

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517MKB1|A0A517MKB1_9BACT Zinc ribbon domain protein OS=Planctomycetes bacterium FF011L OX=1930274 GN=FF011L_41200 PE=4 SV=1
MM1 pKa = 6.96NASRR5 pKa = 11.84FLLIALATTVLPLSASAATMISFDD29 pKa = 4.37EE30 pKa = 4.9LSLSPNSYY38 pKa = 10.76FDD40 pKa = 3.22GHH42 pKa = 6.29GAGASSGTWTSQGATFNTGTYY63 pKa = 9.71GPGWSYY69 pKa = 12.06SNVSDD74 pKa = 3.58NTTAGHH80 pKa = 5.66TNQWSAVPGTDD91 pKa = 3.4ASGSGNYY98 pKa = 9.98VIGTTFSPNGAYY110 pKa = 10.37FNLPGSEE117 pKa = 4.49KK118 pKa = 9.93ISSIAVTNATYY129 pKa = 10.31AYY131 pKa = 10.75LSMLNGDD138 pKa = 4.21AFAKK142 pKa = 10.39EE143 pKa = 4.41FGGTTGDD150 pKa = 4.63DD151 pKa = 3.18EE152 pKa = 6.71DD153 pKa = 4.64FFQVTFTGFDD163 pKa = 3.15SSNAGGSSLGSVDD176 pKa = 6.22FFLADD181 pKa = 3.56YY182 pKa = 10.75RR183 pKa = 11.84FADD186 pKa = 3.59NADD189 pKa = 3.91DD190 pKa = 4.46YY191 pKa = 11.43IIDD194 pKa = 3.4SWQTVDD200 pKa = 5.95LSSLGNARR208 pKa = 11.84SIGISLASSDD218 pKa = 3.26VGMFGMNTPAYY229 pKa = 9.99VAIDD233 pKa = 3.82NIAFQAAAVPEE244 pKa = 4.42PTSLLCIGALVLATSARR261 pKa = 11.84RR262 pKa = 11.84IRR264 pKa = 11.84NRR266 pKa = 11.84FASLASS272 pKa = 3.57

Molecular weight: 28.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517MKP1|A0A517MKP1_9BACT Flagellar basal body rod protein FlgB OS=Planctomycetes bacterium FF011L OX=1930274 GN=FF011L_42610 PE=3 SV=1
MM1 pKa = 7.53AKK3 pKa = 9.9KK4 pKa = 10.07KK5 pKa = 10.23RR6 pKa = 11.84AAKK9 pKa = 8.73TAAKK13 pKa = 9.89KK14 pKa = 9.34VAKK17 pKa = 8.59KK18 pKa = 8.42TAVKK22 pKa = 9.26RR23 pKa = 11.84TAAKK27 pKa = 8.54KK28 pKa = 7.8TAAKK32 pKa = 8.45KK33 pKa = 7.64TAAKK37 pKa = 10.14KK38 pKa = 9.99RR39 pKa = 11.84VAKK42 pKa = 10.12KK43 pKa = 9.63RR44 pKa = 11.84AVKK47 pKa = 10.38KK48 pKa = 9.66VVKK51 pKa = 10.17KK52 pKa = 9.94AAKK55 pKa = 8.46RR56 pKa = 11.84TATKK60 pKa = 10.37KK61 pKa = 9.01VAKK64 pKa = 9.66KK65 pKa = 10.19KK66 pKa = 9.63AVKK69 pKa = 9.11RR70 pKa = 11.84TATKK74 pKa = 10.35KK75 pKa = 9.26VAKK78 pKa = 8.89KK79 pKa = 7.41TAKK82 pKa = 9.78KK83 pKa = 9.53RR84 pKa = 11.84AAKK87 pKa = 10.18KK88 pKa = 9.65RR89 pKa = 11.84VSKK92 pKa = 10.51AAPRR96 pKa = 11.84KK97 pKa = 8.53KK98 pKa = 8.74TATRR102 pKa = 11.84RR103 pKa = 11.84KK104 pKa = 9.55

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5455

0

5455

2043855

30

6821

374.7

41.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.816 ± 0.034

1.098 ± 0.015

6.029 ± 0.039

6.106 ± 0.036

3.685 ± 0.018

7.672 ± 0.049

2.145 ± 0.019

5.106 ± 0.024

3.683 ± 0.036

9.849 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.019

3.265 ± 0.031

5.399 ± 0.027

4.405 ± 0.023

6.417 ± 0.035

6.596 ± 0.026

5.565 ± 0.036

7.024 ± 0.028

1.521 ± 0.018

2.379 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski