Aspergillus pseudotamarii
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13426 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6SZI1|A0A5N6SZI1_ASPPS Uncharacterized protein OS=Aspergillus pseudotamarii OX=132259 GN=BDV38DRAFT_244018 PE=4 SV=1
MM1 pKa = 7.31 VNWTLAGITIQGDD14 pKa = 3.67 VLITSVIIHH23 pKa = 6.32 TLLTLLKK30 pKa = 10.56 LYY32 pKa = 10.58 GLYY35 pKa = 10.76 VLGVLADD42 pKa = 4.66 FAANFQADD50 pKa = 4.01 EE51 pKa = 4.36 LEE53 pKa = 4.17 EE54 pKa = 4.01 WLEE57 pKa = 3.83 NARR60 pKa = 11.84 ARR62 pKa = 11.84 RR63 pKa = 11.84 DD64 pKa = 3.15 NAYY67 pKa = 9.98 AYY69 pKa = 10.9 SSLTDD74 pKa = 3.39 SDD76 pKa = 5.37 LADD79 pKa = 3.63 TDD81 pKa = 3.99 ITDD84 pKa = 4.07 SDD86 pKa = 4.37 YY87 pKa = 11.56 TDD89 pKa = 3.53 SDD91 pKa = 3.61
Molecular weight: 10.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.439
ProMoST 3.783
Dawson 3.681
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.35
Solomon 3.656
Lehninger 3.605
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.783
Patrickios 1.875
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A5N6SRP6|A0A5N6SRP6_ASPPS Peptide-methionine (R)-S-oxide reductase OS=Aspergillus pseudotamarii OX=132259 GN=BDV38DRAFT_284515 PE=3 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.54 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.51 TRR48 pKa = 11.84 LGLL51 pKa = 4.09
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13426
0
13426
6204971
49
9286
462.2
51.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.194 ± 0.018
1.367 ± 0.008
5.538 ± 0.014
5.977 ± 0.02
3.867 ± 0.011
6.819 ± 0.018
2.461 ± 0.009
5.195 ± 0.013
4.533 ± 0.018
9.244 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.193 ± 0.007
3.722 ± 0.01
5.855 ± 0.021
4.011 ± 0.012
5.988 ± 0.017
8.27 ± 0.019
5.96 ± 0.014
6.289 ± 0.014
1.542 ± 0.008
2.976 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here