Mycobacterium phage Doug

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T3P3|A0A5J6T3P3_9CAUD Uncharacterized protein OS=Mycobacterium phage Doug OX=2592659 GN=87 PE=4 SV=1
MM1 pKa = 7.49SHH3 pKa = 5.99EE4 pKa = 4.68HH5 pKa = 7.45DD6 pKa = 4.82EE7 pKa = 4.39IDD9 pKa = 5.25PISQLEE15 pKa = 4.11LDD17 pKa = 3.99VLEE20 pKa = 5.24SGDD23 pKa = 4.97LEE25 pKa = 4.79LATQVGEE32 pKa = 4.3VARR35 pKa = 11.84WAYY38 pKa = 8.26ATTVEE43 pKa = 4.56SLCALLCLPSEE54 pKa = 4.6VAEE57 pKa = 3.99QMVNEE62 pKa = 4.22WFLRR66 pKa = 3.83

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T6H6|A0A5J6T6H6_9CAUD Uncharacterized protein OS=Mycobacterium phage Doug OX=2592659 GN=86 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.68GDD7 pKa = 3.63PTPEE11 pKa = 3.56PRR13 pKa = 11.84VFVRR17 pKa = 11.84PRR19 pKa = 11.84VNALLVRR26 pKa = 11.84CSWCKK31 pKa = 10.07AGVGSRR37 pKa = 11.84CVVAGTDD44 pKa = 3.39VVLRR48 pKa = 11.84RR49 pKa = 11.84SSFHH53 pKa = 6.57DD54 pKa = 3.25VRR56 pKa = 11.84VRR58 pKa = 11.84DD59 pKa = 3.96AEE61 pKa = 4.18LAATGALARR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 3.0

Molecular weight: 8.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

18512

30

1175

187.0

20.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.366 ± 0.481

1.161 ± 0.164

6.553 ± 0.232

6.034 ± 0.302

3.009 ± 0.232

8.843 ± 0.51

2.242 ± 0.203

4.365 ± 0.181

3.425 ± 0.176

7.444 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.121

3.457 ± 0.164

6.164 ± 0.218

3.392 ± 0.217

7.006 ± 0.39

5.764 ± 0.284

6.363 ± 0.236

7.222 ± 0.28

2.317 ± 0.14

2.69 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski