Amino acid dipepetide frequency for Mycobacterium phage Doug

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.992AlaAla: 13.992 ± 1.888
0.972AlaCys: 0.972 ± 0.285
7.347AlaAsp: 7.347 ± 0.577
6.807AlaGlu: 6.807 ± 0.657
2.701AlaPhe: 2.701 ± 0.443
9.346AlaGly: 9.346 ± 1.37
2.431AlaHis: 2.431 ± 0.35
4.538AlaIle: 4.538 ± 0.513
4.808AlaLys: 4.808 ± 0.443
7.887AlaLeu: 7.887 ± 0.771
2.269AlaMet: 2.269 ± 0.42
2.647AlaAsn: 2.647 ± 0.331
5.186AlaPro: 5.186 ± 0.499
3.133AlaGln: 3.133 ± 0.43
6.915AlaArg: 6.915 ± 0.65
5.132AlaSer: 5.132 ± 0.437
5.888AlaThr: 5.888 ± 0.56
6.483AlaVal: 6.483 ± 0.535
2.593AlaTrp: 2.593 ± 0.407
2.593AlaTyr: 2.593 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
0.918CysAla: 0.918 ± 0.283
0.108CysCys: 0.108 ± 0.081
1.08CysAsp: 1.08 ± 0.298
0.81CysGlu: 0.81 ± 0.208
0.162CysPhe: 0.162 ± 0.09
1.513CysGly: 1.513 ± 0.346
0.324CysHis: 0.324 ± 0.123
0.27CysIle: 0.27 ± 0.129
0.486CysLys: 0.486 ± 0.171
0.864CysLeu: 0.864 ± 0.239
0.054CysMet: 0.054 ± 0.052
0.378CysAsn: 0.378 ± 0.153
1.026CysPro: 1.026 ± 0.258
0.216CysGln: 0.216 ± 0.106
0.756CysArg: 0.756 ± 0.28
0.702CysSer: 0.702 ± 0.234
0.54CysThr: 0.54 ± 0.187
0.864CysVal: 0.864 ± 0.195
0.378CysTrp: 0.378 ± 0.122
0.162CysTyr: 0.162 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
7.131AspAla: 7.131 ± 0.686
0.54AspCys: 0.54 ± 0.136
4.214AspAsp: 4.214 ± 0.509
3.782AspGlu: 3.782 ± 0.559
1.567AspPhe: 1.567 ± 0.223
6.159AspGly: 6.159 ± 0.543
1.243AspHis: 1.243 ± 0.213
2.107AspIle: 2.107 ± 0.339
1.621AspLys: 1.621 ± 0.295
6.213AspLeu: 6.213 ± 0.45
0.864AspMet: 0.864 ± 0.217
1.783AspAsn: 1.783 ± 0.395
5.078AspPro: 5.078 ± 0.623
2.539AspGln: 2.539 ± 0.304
5.024AspArg: 5.024 ± 0.63
3.565AspSer: 3.565 ± 0.489
3.998AspThr: 3.998 ± 0.408
4.7AspVal: 4.7 ± 0.571
1.405AspTrp: 1.405 ± 0.285
1.999AspTyr: 1.999 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
5.996GluAla: 5.996 ± 0.564
0.918GluCys: 0.918 ± 0.278
3.079GluAsp: 3.079 ± 0.307
3.187GluGlu: 3.187 ± 0.499
2.161GluPhe: 2.161 ± 0.317
3.295GluGly: 3.295 ± 0.418
1.513GluHis: 1.513 ± 0.33
2.431GluIle: 2.431 ± 0.344
2.215GluLys: 2.215 ± 0.294
5.618GluLeu: 5.618 ± 0.669
1.729GluMet: 1.729 ± 0.358
1.783GluAsn: 1.783 ± 0.247
2.809GluPro: 2.809 ± 0.39
2.809GluGln: 2.809 ± 0.339
5.402GluArg: 5.402 ± 0.613
3.025GluSer: 3.025 ± 0.415
3.998GluThr: 3.998 ± 0.611
4.592GluVal: 4.592 ± 0.559
1.675GluTrp: 1.675 ± 0.291
2.107GluTyr: 2.107 ± 0.331
0.0GluXaa: 0.0 ± 0.0
Phe
3.025PheAla: 3.025 ± 0.404
0.216PheCys: 0.216 ± 0.113
2.539PheAsp: 2.539 ± 0.448
1.459PheGlu: 1.459 ± 0.299
0.972PhePhe: 0.972 ± 0.297
3.079PheGly: 3.079 ± 0.651
0.756PheHis: 0.756 ± 0.232
1.297PheIle: 1.297 ± 0.334
1.08PheLys: 1.08 ± 0.249
1.675PheLeu: 1.675 ± 0.26
0.756PheMet: 0.756 ± 0.217
1.405PheAsn: 1.405 ± 0.351
1.459PhePro: 1.459 ± 0.309
1.243PheGln: 1.243 ± 0.263
1.459PheArg: 1.459 ± 0.249
1.567PheSer: 1.567 ± 0.352
2.431PheThr: 2.431 ± 0.319
2.107PheVal: 2.107 ± 0.306
0.702PheTrp: 0.702 ± 0.177
0.864PheTyr: 0.864 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
9.022GlyAla: 9.022 ± 1.122
1.243GlyCys: 1.243 ± 0.279
5.726GlyAsp: 5.726 ± 0.592
4.106GlyGlu: 4.106 ± 0.479
2.539GlyPhe: 2.539 ± 0.464
10.966GlyGly: 10.966 ± 2.111
1.891GlyHis: 1.891 ± 0.342
3.836GlyIle: 3.836 ± 0.642
2.593GlyLys: 2.593 ± 0.409
6.05GlyLeu: 6.05 ± 0.514
2.161GlyMet: 2.161 ± 0.409
3.187GlyAsn: 3.187 ± 0.435
4.106GlyPro: 4.106 ± 0.65
2.431GlyGln: 2.431 ± 0.563
5.672GlyArg: 5.672 ± 0.759
6.267GlySer: 6.267 ± 0.848
6.159GlyThr: 6.159 ± 0.843
5.726GlyVal: 5.726 ± 0.576
2.323GlyTrp: 2.323 ± 0.371
2.431GlyTyr: 2.431 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.729HisAla: 1.729 ± 0.336
0.27HisCys: 0.27 ± 0.145
1.08HisAsp: 1.08 ± 0.211
1.459HisGlu: 1.459 ± 0.302
0.486HisPhe: 0.486 ± 0.142
1.999HisGly: 1.999 ± 0.357
0.972HisHis: 0.972 ± 0.31
1.513HisIle: 1.513 ± 0.307
1.026HisLys: 1.026 ± 0.318
1.405HisLeu: 1.405 ± 0.279
0.486HisMet: 0.486 ± 0.124
0.972HisAsn: 0.972 ± 0.254
1.243HisPro: 1.243 ± 0.22
0.756HisGln: 0.756 ± 0.229
1.891HisArg: 1.891 ± 0.332
0.918HisSer: 0.918 ± 0.217
1.459HisThr: 1.459 ± 0.371
1.351HisVal: 1.351 ± 0.299
0.486HisTrp: 0.486 ± 0.169
0.918HisTyr: 0.918 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.484IleAla: 4.484 ± 0.542
0.81IleCys: 0.81 ± 0.249
3.782IleAsp: 3.782 ± 0.482
3.673IleGlu: 3.673 ± 0.339
0.756IlePhe: 0.756 ± 0.209
3.998IleGly: 3.998 ± 0.426
1.513IleHis: 1.513 ± 0.31
1.297IleIle: 1.297 ± 0.259
1.188IleLys: 1.188 ± 0.269
2.107IleLeu: 2.107 ± 0.417
0.324IleMet: 0.324 ± 0.144
1.567IleAsn: 1.567 ± 0.272
3.079IlePro: 3.079 ± 0.324
1.567IleGln: 1.567 ± 0.27
2.269IleArg: 2.269 ± 0.381
2.161IleSer: 2.161 ± 0.494
3.565IleThr: 3.565 ± 0.414
3.403IleVal: 3.403 ± 0.435
1.08IleTrp: 1.08 ± 0.217
0.972IleTyr: 0.972 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
3.782LysAla: 3.782 ± 0.426
0.432LysCys: 0.432 ± 0.132
2.053LysAsp: 2.053 ± 0.316
1.188LysGlu: 1.188 ± 0.214
1.351LysPhe: 1.351 ± 0.238
2.323LysGly: 2.323 ± 0.355
1.08LysHis: 1.08 ± 0.288
0.81LysIle: 0.81 ± 0.239
1.675LysLys: 1.675 ± 0.421
2.863LysLeu: 2.863 ± 0.565
0.756LysMet: 0.756 ± 0.196
1.08LysAsn: 1.08 ± 0.225
2.701LysPro: 2.701 ± 0.447
1.459LysGln: 1.459 ± 0.228
2.593LysArg: 2.593 ± 0.378
2.053LysSer: 2.053 ± 0.313
2.053LysThr: 2.053 ± 0.402
2.161LysVal: 2.161 ± 0.394
0.864LysTrp: 0.864 ± 0.249
0.972LysTyr: 0.972 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
7.725LeuAla: 7.725 ± 0.768
0.594LeuCys: 0.594 ± 0.226
4.646LeuAsp: 4.646 ± 0.565
4.322LeuGlu: 4.322 ± 0.552
2.647LeuPhe: 2.647 ± 0.331
5.402LeuGly: 5.402 ± 0.561
0.864LeuHis: 0.864 ± 0.247
3.133LeuIle: 3.133 ± 0.407
2.215LeuLys: 2.215 ± 0.355
4.808LeuLeu: 4.808 ± 0.57
1.783LeuMet: 1.783 ± 0.311
2.647LeuAsn: 2.647 ± 0.379
5.726LeuPro: 5.726 ± 0.653
3.295LeuGln: 3.295 ± 0.431
5.186LeuArg: 5.186 ± 0.622
4.7LeuSer: 4.7 ± 0.462
5.78LeuThr: 5.78 ± 0.531
5.24LeuVal: 5.24 ± 0.544
1.351LeuTrp: 1.351 ± 0.286
2.377LeuTyr: 2.377 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
1.999MetAla: 1.999 ± 0.337
0.324MetCys: 0.324 ± 0.185
1.405MetAsp: 1.405 ± 0.299
0.864MetGlu: 0.864 ± 0.209
0.648MetPhe: 0.648 ± 0.197
1.729MetGly: 1.729 ± 0.288
0.162MetHis: 0.162 ± 0.096
0.918MetIle: 0.918 ± 0.251
0.648MetLys: 0.648 ± 0.168
2.053MetLeu: 2.053 ± 0.293
0.486MetMet: 0.486 ± 0.176
0.918MetAsn: 0.918 ± 0.213
1.243MetPro: 1.243 ± 0.253
0.486MetGln: 0.486 ± 0.134
1.675MetArg: 1.675 ± 0.308
2.647MetSer: 2.647 ± 0.417
1.945MetThr: 1.945 ± 0.272
1.08MetVal: 1.08 ± 0.323
0.27MetTrp: 0.27 ± 0.127
0.324MetTyr: 0.324 ± 0.107
0.0MetXaa: 0.0 ± 0.0
Asn
3.619AsnAla: 3.619 ± 0.408
0.324AsnCys: 0.324 ± 0.122
1.675AsnAsp: 1.675 ± 0.262
1.729AsnGlu: 1.729 ± 0.326
0.702AsnPhe: 0.702 ± 0.262
4.16AsnGly: 4.16 ± 0.544
1.08AsnHis: 1.08 ± 0.238
1.675AsnIle: 1.675 ± 0.501
0.864AsnLys: 0.864 ± 0.259
2.755AsnLeu: 2.755 ± 0.413
0.648AsnMet: 0.648 ± 0.198
1.999AsnAsn: 1.999 ± 0.359
2.809AsnPro: 2.809 ± 0.347
1.026AsnGln: 1.026 ± 0.323
2.431AsnArg: 2.431 ± 0.448
1.621AsnSer: 1.621 ± 0.272
2.215AsnThr: 2.215 ± 0.27
1.891AsnVal: 1.891 ± 0.291
0.648AsnTrp: 0.648 ± 0.165
0.702AsnTyr: 0.702 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
5.402ProAla: 5.402 ± 0.592
0.702ProCys: 0.702 ± 0.228
4.268ProAsp: 4.268 ± 0.458
4.754ProGlu: 4.754 ± 0.51
1.675ProPhe: 1.675 ± 0.275
6.429ProGly: 6.429 ± 0.802
1.513ProHis: 1.513 ± 0.303
2.161ProIle: 2.161 ± 0.318
2.161ProLys: 2.161 ± 0.411
4.646ProLeu: 4.646 ± 0.555
1.459ProMet: 1.459 ± 0.343
1.945ProAsn: 1.945 ± 0.325
4.538ProPro: 4.538 ± 0.665
2.377ProGln: 2.377 ± 0.391
3.511ProArg: 3.511 ± 0.688
3.457ProSer: 3.457 ± 0.468
3.133ProThr: 3.133 ± 0.484
4.808ProVal: 4.808 ± 0.517
1.134ProTrp: 1.134 ± 0.256
1.567ProTyr: 1.567 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
4.268GlnAla: 4.268 ± 0.567
0.27GlnCys: 0.27 ± 0.144
1.513GlnAsp: 1.513 ± 0.273
1.783GlnGlu: 1.783 ± 0.322
1.134GlnPhe: 1.134 ± 0.23
2.593GlnGly: 2.593 ± 0.475
1.134GlnHis: 1.134 ± 0.245
1.945GlnIle: 1.945 ± 0.319
1.08GlnLys: 1.08 ± 0.218
2.755GlnLeu: 2.755 ± 0.443
0.864GlnMet: 0.864 ± 0.209
0.972GlnAsn: 0.972 ± 0.236
2.593GlnPro: 2.593 ± 0.399
1.026GlnGln: 1.026 ± 0.198
2.539GlnArg: 2.539 ± 0.416
2.215GlnSer: 2.215 ± 0.34
1.675GlnThr: 1.675 ± 0.362
2.215GlnVal: 2.215 ± 0.328
0.54GlnTrp: 0.54 ± 0.156
0.81GlnTyr: 0.81 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
6.591ArgAla: 6.591 ± 0.711
1.243ArgCys: 1.243 ± 0.382
4.484ArgAsp: 4.484 ± 0.542
5.24ArgGlu: 5.24 ± 0.642
2.593ArgPhe: 2.593 ± 0.446
4.322ArgGly: 4.322 ± 0.463
0.81ArgHis: 0.81 ± 0.226
4.7ArgIle: 4.7 ± 0.59
2.215ArgLys: 2.215 ± 0.391
4.916ArgLeu: 4.916 ± 0.556
2.377ArgMet: 2.377 ± 0.412
2.323ArgAsn: 2.323 ± 0.443
4.43ArgPro: 4.43 ± 0.57
1.621ArgGln: 1.621 ± 0.318
5.942ArgArg: 5.942 ± 0.836
3.998ArgSer: 3.998 ± 0.415
2.863ArgThr: 2.863 ± 0.473
5.132ArgVal: 5.132 ± 0.61
2.161ArgTrp: 2.161 ± 0.417
2.107ArgTyr: 2.107 ± 0.382
0.0ArgXaa: 0.0 ± 0.0
Ser
5.186SerAla: 5.186 ± 0.829
0.324SerCys: 0.324 ± 0.155
3.782SerAsp: 3.782 ± 0.509
2.917SerGlu: 2.917 ± 0.415
2.323SerPhe: 2.323 ± 0.401
6.159SerGly: 6.159 ± 0.892
1.188SerHis: 1.188 ± 0.26
2.971SerIle: 2.971 ± 0.465
2.431SerLys: 2.431 ± 0.342
4.214SerLeu: 4.214 ± 0.439
1.08SerMet: 1.08 ± 0.253
2.485SerAsn: 2.485 ± 0.435
3.349SerPro: 3.349 ± 0.294
1.405SerGln: 1.405 ± 0.229
3.457SerArg: 3.457 ± 0.493
3.511SerSer: 3.511 ± 0.664
3.295SerThr: 3.295 ± 0.472
4.754SerVal: 4.754 ± 0.592
1.134SerTrp: 1.134 ± 0.236
1.675SerTyr: 1.675 ± 0.285
0.0SerXaa: 0.0 ± 0.0
Thr
6.483ThrAla: 6.483 ± 0.595
0.486ThrCys: 0.486 ± 0.174
3.998ThrAsp: 3.998 ± 0.572
3.89ThrGlu: 3.89 ± 0.433
1.783ThrPhe: 1.783 ± 0.29
6.375ThrGly: 6.375 ± 0.766
1.675ThrHis: 1.675 ± 0.296
3.187ThrIle: 3.187 ± 0.459
1.945ThrLys: 1.945 ± 0.331
4.214ThrLeu: 4.214 ± 0.5
1.134ThrMet: 1.134 ± 0.238
2.269ThrAsn: 2.269 ± 0.406
3.89ThrPro: 3.89 ± 0.415
1.999ThrGln: 1.999 ± 0.376
4.106ThrArg: 4.106 ± 0.456
3.511ThrSer: 3.511 ± 0.501
4.16ThrThr: 4.16 ± 0.517
5.78ThrVal: 5.78 ± 0.575
1.134ThrTrp: 1.134 ± 0.274
1.621ThrTyr: 1.621 ± 0.302
0.0ThrXaa: 0.0 ± 0.0
Val
7.293ValAla: 7.293 ± 0.592
1.026ValCys: 1.026 ± 0.233
5.726ValAsp: 5.726 ± 0.607
5.024ValGlu: 5.024 ± 0.654
2.323ValPhe: 2.323 ± 0.413
5.402ValGly: 5.402 ± 0.658
1.513ValHis: 1.513 ± 0.322
2.539ValIle: 2.539 ± 0.411
2.539ValLys: 2.539 ± 0.349
5.132ValLeu: 5.132 ± 0.576
1.351ValMet: 1.351 ± 0.239
2.647ValAsn: 2.647 ± 0.4
3.619ValPro: 3.619 ± 0.448
2.701ValGln: 2.701 ± 0.342
4.754ValArg: 4.754 ± 0.639
4.43ValSer: 4.43 ± 0.634
5.186ValThr: 5.186 ± 0.597
5.888ValVal: 5.888 ± 0.673
1.621ValTrp: 1.621 ± 0.316
1.513ValTyr: 1.513 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
2.107TrpAla: 2.107 ± 0.289
0.432TrpCys: 0.432 ± 0.139
1.243TrpAsp: 1.243 ± 0.219
1.405TrpGlu: 1.405 ± 0.332
0.81TrpPhe: 0.81 ± 0.179
0.972TrpGly: 0.972 ± 0.221
0.486TrpHis: 0.486 ± 0.156
1.08TrpIle: 1.08 ± 0.25
0.702TrpLys: 0.702 ± 0.159
1.891TrpLeu: 1.891 ± 0.325
1.026TrpMet: 1.026 ± 0.27
0.594TrpAsn: 0.594 ± 0.222
1.243TrpPro: 1.243 ± 0.323
0.918TrpGln: 0.918 ± 0.282
2.323TrpArg: 2.323 ± 0.451
1.188TrpSer: 1.188 ± 0.236
1.459TrpThr: 1.459 ± 0.215
1.783TrpVal: 1.783 ± 0.419
1.026TrpTrp: 1.026 ± 0.205
0.486TrpTyr: 0.486 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.917TyrAla: 2.917 ± 0.43
0.378TyrCys: 0.378 ± 0.143
1.891TyrAsp: 1.891 ± 0.371
1.945TyrGlu: 1.945 ± 0.314
0.756TyrPhe: 0.756 ± 0.19
2.215TyrGly: 2.215 ± 0.378
0.27TyrHis: 0.27 ± 0.101
1.243TyrIle: 1.243 ± 0.243
0.756TyrLys: 0.756 ± 0.205
2.377TyrLeu: 2.377 ± 0.367
0.162TyrMet: 0.162 ± 0.071
0.972TyrAsn: 0.972 ± 0.223
1.513TyrPro: 1.513 ± 0.237
0.918TyrGln: 0.918 ± 0.226
2.161TyrArg: 2.161 ± 0.412
0.972TyrSer: 0.972 ± 0.195
1.837TyrThr: 1.837 ± 0.322
2.269TyrVal: 2.269 ± 0.358
0.648TyrTrp: 0.648 ± 0.166
0.702TyrTyr: 0.702 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (18512 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski