Anopheles darlingi (Mosquito)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicomorpha;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5JVS8|W5JVS8_ANODA Uncharacterized protein OS=Anopheles darlingi OX=43151 GN=AND_001243 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 5.28NFSNPLVVTTIPALWRR18 pKa = 11.84LVWTLWSKK26 pKa = 11.05QFMSINDD33 pKa = 3.77YY34 pKa = 11.19SSSCYY39 pKa = 9.84QCWSVCRR46 pKa = 11.84YY47 pKa = 9.59CGGWHH52 pKa = 5.8LTFPYY57 pKa = 10.16VALGVALILWPHH69 pKa = 6.52RR70 pKa = 11.84LWLSHH75 pKa = 5.52VAGGLLIGLGMLRR88 pKa = 11.84LLTVCKK94 pKa = 10.28SRR96 pKa = 11.84PHH98 pKa = 6.51SKK100 pKa = 10.66DD101 pKa = 4.11DD102 pKa = 3.44EE103 pKa = 4.54LLNHH107 pKa = 6.85FDD109 pKa = 3.99EE110 pKa = 5.32QVDD113 pKa = 4.21RR114 pKa = 11.84YY115 pKa = 11.5DD116 pKa = 3.52NLSDD120 pKa = 3.82VLVDD124 pKa = 4.84DD125 pKa = 5.14SMPEE129 pKa = 3.98PGQSLEE135 pKa = 5.54DD136 pKa = 5.2DD137 pKa = 5.11DD138 pKa = 7.07DD139 pKa = 6.08PDD141 pKa = 6.67DD142 pKa = 5.77DD143 pKa = 5.93VNDD146 pKa = 4.85DD147 pKa = 4.69DD148 pKa = 6.54DD149 pKa = 6.28VDD151 pKa = 4.82VDD153 pKa = 6.11DD154 pKa = 7.02DD155 pKa = 6.27DD156 pKa = 7.81DD157 pKa = 7.42DD158 pKa = 7.16DD159 pKa = 7.43DD160 pKa = 6.28DD161 pKa = 4.51VALHH165 pKa = 6.26TGGII169 pKa = 3.97

Molecular weight: 19.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5JTN6|W5JTN6_ANODA Serine-type enodpeptidase OS=Anopheles darlingi OX=43151 GN=AND_000482 PE=3 SV=1
MM1 pKa = 7.11YY2 pKa = 9.64PGVSRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 10.11DD10 pKa = 3.23EE11 pKa = 4.48FGGSEE16 pKa = 4.09GSKK19 pKa = 10.4DD20 pKa = 3.69RR21 pKa = 11.84RR22 pKa = 11.84PTFLNSLRR30 pKa = 11.84QSIDD34 pKa = 3.01HH35 pKa = 6.44PTQDD39 pKa = 3.62RR40 pKa = 11.84QASAAGGEE48 pKa = 3.83IGAISFEE55 pKa = 4.14RR56 pKa = 11.84SACQLEE62 pKa = 4.41GVYY65 pKa = 9.8IASHH69 pKa = 6.71RR70 pKa = 11.84ISRR73 pKa = 11.84SRR75 pKa = 11.84DD76 pKa = 2.67RR77 pKa = 11.84RR78 pKa = 11.84AFKK81 pKa = 9.97TGKK84 pKa = 9.75LKK86 pKa = 9.85CTSGMIEE93 pKa = 5.45FIGHH97 pKa = 5.72WFRR100 pKa = 11.84SVKK103 pKa = 10.24EE104 pKa = 3.79RR105 pKa = 11.84VDD107 pKa = 3.63RR108 pKa = 11.84LKK110 pKa = 11.08LKK112 pKa = 10.05PDD114 pKa = 3.33YY115 pKa = 10.54RR116 pKa = 11.84GSSTSNRR123 pKa = 11.84SRR125 pKa = 11.84YY126 pKa = 9.36IPLEE130 pKa = 4.03AEE132 pKa = 4.86AISSLRR138 pKa = 11.84RR139 pKa = 11.84GSRR142 pKa = 11.84WLSLRR147 pKa = 11.84CGVSASDD154 pKa = 3.93EE155 pKa = 4.29RR156 pKa = 11.84QLSAGCARR164 pKa = 4.2

Molecular weight: 18.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10447

0

10447

6035843

33

8216

577.8

64.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.502 ± 0.024

1.826 ± 0.021

5.309 ± 0.019

6.274 ± 0.03

3.412 ± 0.019

6.774 ± 0.036

2.71 ± 0.016

4.938 ± 0.02

5.137 ± 0.025

8.881 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.011

4.42 ± 0.017

5.356 ± 0.025

4.873 ± 0.033

5.748 ± 0.021

8.299 ± 0.039

6.134 ± 0.02

6.218 ± 0.02

0.98 ± 0.007

2.946 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski